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研究生:陳盈如
研究生(外文):Ying-Ru Chen
論文名稱:含幾丁聚醣粒子溫感型共聚物
論文名稱(外文):Thermosensitive copolymer with chitosan particals
指導教授:鄭國忠鄭國忠引用關係
指導教授(外文):Kuo-Chung Cheng
口試委員:林昕怡陳明仁董崇明
口試委員(外文):Hsin-Yi LinChen, Ming-JenTrong-Ming Don
口試日期:2012-07-28
學位類別:碩士
校院名稱:國立臺北科技大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:73
中文關鍵詞:氮-異丙基丙烯醯胺聚乙二醇幾丁聚醣溫感型共聚物生物性密封劑
外文關鍵詞:NIPAAmPEGMEAchitosanbio-sealantthermo-sensitive copolymer
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本研究首先將溫感型氮-異丙基丙烯醯胺(NIPAAm)單體及聚乙烯醇甲基丙烯酸酯(PEGMEA)單體,以雙氧水-維他命C作氧化還原自由基起始,合成不同組成比之溫感型共聚物水膠。我們將合成之共聚物與幾丁聚醣粒子混合,並以磷酸緩衝溶液(PBS)中為溶劑,配置不同重量百分比濃度(w/w%)之樣品。藉由倒瓶測試、DSC、穿透率測試,觀察其流體性質以及霧點(cloud point)。發現合成之poly(NIPAAm)其相轉換溫度為27℃;共聚物Poly(NIPAAm-PEGMEA)中的PEGMEA增加,其相轉換溫度可提升至36.4℃。樣品中若混有幾丁聚醣微粒,所測得之相轉換溫度並無重大差異。poly(NIPAAm)在緩衝溶液37℃時,一小時後之厚度變化約40%而poly(NIPAAm-pegmea)上升至80%,但加入幾丁聚醣之樣品厚度變化只有55%。MTT assay細胞活性測試發現所合成之高分子材料,並未發現會降低細胞存活率,隨著幾丁聚醣微粒添加量的增加,細胞存活率有上升的趨勢。

Poly(NIPAAm-PEGMEA) copolymers were prepared via free radical polymerization of various mole ratios of monomers initialized by H2O2-vitamin C redox initiators. The synthesized copolymers were further mixed with chitosan particles in phosphate buffered saline (PBS). The temperature dependences of flow property and cloud point of the solution with chitosan particles were investigated by the tube-invert, DSC, and light transmission tests. It was found that the poly(NIPAAm) could flow at 25oC, and formed a gel at higher temperature. The cloud point of the Poly(NIPAAm-PEGMEA) could increased to about 36.4oC with increasing of PEGMEA unit, but there had almost no change with addition of chitosan particles. The thickness of the poly(NIPAAm), and poly(NIPAAm-PEGMEA) hydrogels could increased to 40% and 80%, respectively, higher than that before swelling in PBS at 37oC. The ratio became only 50% for the poly(NIPAAm-PEGMEA)/chitosan particle system. The MTT assay indicated the biocompatibility could be improved by adding the chitosan particles into the copolymer hydrogel.

摘 要 ........................................i
Abstract ........................................ii
目錄 ........................................iv
表目錄 ........................................vii
圖目錄 ........................................viii
附錄圖 ........................................x
第一章 緒論 ........................................1
1.1研究動機與目的 ........................................2
1.2研究方法 ........................................3
1.3預期目標 ........................................3
2.1 環境感應型分子........................................ 4
2.1.1 環境型感應型分子之介紹與分類........................................4
2.1.2 酸鹼值感應型分子........................................8
2.1.3溫度感應型分子(thermo-sensitive polymer)........................................11
2.1.4 溫度/酸鹼值感應型分子 ........................................14
2.1.5 異丙基丙烯醯胺之簡介 ........................................15
2.1.6 聚乙二醇甲基丙烯酸酯之簡介........................................16
2.1.7幾丁聚醣之簡介........................................17
3.1實驗材料 ........................................21
3.1.1實驗藥品 ........................................21
3.1.2 實驗儀器 ........................................24
3.2實驗流程與步驟 ........................................26
3.2.1實驗流程 ........................................26
3.2.2 NIPAAm之純化方法 ........................................27
3.2.3溫感型高分子之合成與純化 ........................................27
3.2.4 溫感型高分子之產率 ........................................29
3.2.5溫感型高分子之FT-IR分析 ........................................29
3.2.6微分掃描熱卡計(DSC) ........................................29
3.2.7配製磷酸鹽緩衝溶液(PBS) ........................................30
3.2.8合成之溫度感應型分子sol-gel 轉變測試 ........................................30
3.2.9穿透率測試(transmittance) ........................................30
3.2.10溫感型高分子之樣品製備 ........................................31
3.2.11膨潤率(swelling ratio) ........................................31
3.2.12細胞活性測試 ........................................32
3.2.13水解降解測試(hydrolysis) ........................................33
第四章 結果與討論 ........................................34
4.1合成溫感型高分子 ........................................34
4.2合成溫感型高分子之產率 ........................................37
4.3合成之溫感型高分子之FT-IR ........................................38
4.4溫感型高分子之倒瓶實驗 ........................................39
4.7合成之溫感型高分子之LCST以及穿透率測試 ........................................46
4.8溫感型高分子之膨潤度 ........................................48
4.9溫感型高分子之細胞活性分析........................................49
第五章 結論........................................60
未來工作 ........................................62
參考文獻 ........................................63
附錄 ........................................68




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