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研究生:張偉倫
研究生(外文):Wei-Lun Zhang
論文名稱:頻閃式光學映射系統之研製
論文名稱(外文):Development of Strobscopic optical mapping system
指導教授:陳亮嘉
指導教授(外文):Liang-Chia Chan
口試委員:賴宇俊葉勝利
口試委員(外文):Yu-Jun LaiSheng-Lih Yeh
口試日期:2011-07-27
學位類別:碩士
校院名稱:國立臺北科技大學
系所名稱:自動化科技研究所
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:82
中文關鍵詞:光學映射電壓敏感性螢光染劑心臟電生理頻閃技術動作電位
外文關鍵詞:Optical mappingvoltage-sensitive fluorescent dyecardiac electrophysiologystroboscopic techniquesaction potentials
相關次數:
  • 被引用被引用:0
  • 點閱點閱:262
  • 評分評分:
  • 下載下載:5
  • 收藏至我的研究室書目清單書目收藏:1
光學映像系統,為研究心臟電生理之儀器,本研究將電壓敏感性染劑添加於心臟,在照射能量於心臟表面後激發螢光,心臟細胞離子濃度伴隨電壓變化使螢光光譜產生飄移,螢光透過光學系統後成像於相機,利用記錄心臟影像光強變化對映之細胞內離子流動。由於心臟離子流動及傳導非常快速,需要用高速擷取影相設備,方能記錄短時間內之變化,因此,在高速取像的限制條件下,傳統光學映像系統無法有細緻的空間解析度,且售價昂貴。本研究提出頻閃技術,利用心臟動作電位重複之特性,解決高速取像之使用限制,使系統擁有大範圍之時間與空間解析度,利於心臟動作電位傳導之研究。另一方面,心臟電生理的生理數據整理及統計上,相關程式設計及開發方面的研究依然十分缺乏,現有的系統在使用上,局限於其所開發的軟體,後端使用者無法根據其所需變化精進。因此,本研究開發一套光學式影像映像系統,目的於降低其系統造價,搭配頻閃技術調配控制影像擷取器取像,研發高時間與空間解析度及即時影像的影像映像光學硬體系統,並將我們所研發的光學影像映像系統用於觀察及研究小鼠心臟生理特性研究上。

Optical Mapping is one of instruments in the field of cardiac electrophysiolog. The measurement is performed by firstly injecting electro-fluorescent dye into the heart tissues. Surface of the heart tissues emit fluorescent light when there is a action potential change and the emitted light is then captured by camera through an imaging system. The distribution and flow of the action potential on the surface of the heart tissues are evaluated from the captured images by analyzing variation and direction of the intensity variation in time and spatial domain. The electric current flow inside the tissue is very fast, therefore high speed imaging system must be employed and the phenomenon can only be recorded in a short period of time. The drawback is the spatial resolution has to be sacrificed and the price of the optical mapping systems is very high. The objective of this research is to build an optical mapping system with reasonable price without having to sacrifice the time and spatial resolution. The developed optical mapping system is equipped with stroboscopic lighting which is synchronized with the image acquisition system and also a real-time image acquisition system. Stroboscopic lighting is employed to overcome the limitation of camera speed by recombining the captured periodic optical signal. Therefore, the developed system possesses high resolution in both time and space compared to the conventional optical mapping system. Heart tissue of an experimental rat is employed in the experiment to evaluate the feasibility of the proposed method.

摘 要 i
ABSTRACT ii
誌謝 iv
目錄 v
表目錄 viii
圖目錄 ix
第1章 緒論 1
1.1 研究背景 1
1.2 研究目的 2
1.3 研究之特點 3
1.4 論文架構 4
第2章 理論背景與文獻回顧 5
2.1 心臟 5
2.2 心肌細胞電生理特性 6
2.2.1 心肌細胞 6
2.2.2 細胞膜電位 6
2.2.3 靜止膜電位 8
2.2.4 動作電位 9
2.2.5 心肌細胞的電生理 12
2.3 量測心臟電特性的工具 14
2.3.1 心電圖 14
2.3.2 微電極量測 15
2.4 光學式膜電位量測方式 16
2.5 文獻回顧結論 19
第3章 頻閃式光學映射技術原理 20
3.1 光譜 20
3.2 螢光發光原理 21
3.3 電場敏感性染劑di-4-ANEPPS 23
3.4 光學映射原理 25
3.5 頻閃技術 26
3.6 影像訊號處理與分析 28
3.6.1 影像格式 28
3.6.2 訊號正規化 28
3.6.3 低通濾波器 29
3.6.4 高斯糢糊 30
3.6.5 中值濾波 30
3.6.6 傅立葉轉換 32
3.6.7 巴特沃斯濾波 33
第4章 量測系統架構 35
4.1 硬體架構及選用 35
4.1.1 影像擷取系統 36
4.1.2 光源系統 38
4.1.3 顯微鏡系統 39
4.1.4 硬體機構設計 40
4.1.5 外部觸發頻閃控制模組 41
4.1.6 電子刺極器 45
4.1.7 循環系統 46
4.1.8 心電圖 47
4.2 軟體系統介紹 48
4.2.1 取像軟體介紹 48
4.2.2 分析軟體 49
4.3 頻閃控制器製作 50
4.3.1 頻閃外部觸發器及動作程序說明 51
4.3.2 頻閃控制器測試實驗架設 52
第5章 實驗結果與分析 54
5.1 頻閃控制器之驗證 54
5.2 光學映射實驗 62
5.2.1 離體心臟準備流程 63
5.2.2 訊號濾波處理 65
5.2.3 即時光學映射量測 67
5.2.4 頻閃式光學映射量測 71
第6章 結論與未來展望 75
6.1 結論 75
6.1.1 頻閃式光學映射系統之特點 75
6.1.2 研究成果 78
6.2 未來展望 79
參考文獻 80


[1]行政院衛生署統計民國九十九年十大死因統計
[2]Aswini Kumar, “ECG in 100 steps” Life Hugger, 2009.
[3]M. Rivera-Ruiz, C. Cajavilca, J. Varon, “Einthoven''s String Galvanometer,” Tex Heart Inst J. Vol. 35, pp.174–178, 2008.
[4]Yu-Jun Lai et al. “On the mechanisms of arrhythmias in the myocardium of mxinα-deficient murine left atrial-pulmonary veins tissue,” Life Sci, Vol. 83, pp.272–283, 2008
[5]L. B. COHEN, R. D. KEYNES & BERTIL HILLE. “Light scattering and birefringence changes during nerve activity.” Nature, Vol. 218, pp.438-441, 1968.
[6]F. Qu,Crystal, M. Ripplinger, Vladimir P. Nikolski, “Three-dimensional panoramic imaging of cardiac arrhythmias in rabbit heart,” Journal of Biomedical Optics, Vol. 4 pp. 044019, 2007.
[7]David S. Rosenbaum, “Optical mapping of cardiac excitation and arrhythmias, “Wiley-Blackwell, 2001.
[8]S. Lin and J. P. Wikswo, “Panoramic optical imaging of electrical propagation in isolated heart,” Journal of biomedical optics, Vol. 4, pp. 200-207, 1999.
[9]M. Bray, “Three-dimensional surface reconstruction and fluorescent visualization of cardiac activation,” Biomedical Engineering, IEEE, Vol. 44, pp. 1382 – 1391, 2000.
[10]W. T. Baxtera, S. F. Mironova, A. V. Zaitseva, J. Jalife and A. M. Pertsova “Visualizing excitation waves inside cardiac muscle using transillumination” Biophysical Journal, Vol. 80, pp. 516-530, 2001.
[11]D. Y. Tanga and Y. Lia, “Preliminary study of a dual CCD based ratiometric optical mapping system” Proc. SPIE, Vol. 4958, pp. 1605-7522, 2003.
[12]M. W. Kay, P. M. Amison, and J. M. Rogers, “Three-dimensional surface reconstruction and panoramic optical mapping of large hearts” IEEE Trans Biomed Eng, Vol.51, No. 7, 2004.
[13]Inagaki M, Hidaka I, Aiba T, Tatewaki T, Sunagawa K, Sugimachi M. “High resolution optical mapping of cardiac action potentials in freely beating rabbit hearts,” Conf Proc IEEE Eng Med Biol Soc, Vol. 5, pp. 3578-3580, 2004.
[14]Takata Y, Sato S, Nashimoto S, Yamaguchi T, Arafune T, Sakuma I, Shibata N, Honjo H, Kodama I. “Development of optical mapping system with real-time feedback stimulation in the heart,” Conf Proc IEEE Eng Med Biol Soc, Vol. 2, pp.1618-21, 2005.
[15]Svrcek M, Rutherford S, Chen AY, Provaznik I, Smaill B. “Characteristics of motion artifacts in cardiac optical mapping studies,” Conf Proc IEEE Eng Med Biol Soc, Vol. 7, pp.3240-3423, 2009.
[16]Yuan X, Uyanik I, Situ N, Xi Y, Cheng J. “ COI-Wiz: an interactive computer wizard for analyzing cardiac optical signals,” Conf Proc IEEE Eng Med Biol Soc. pp.1828-31, 2009.
[17]M. Svrcek, S. Rutherford, A. Y. H. Chen, I. Provaznik, B. H. Smaill , “Using image registration to reconstruct spatiotemporal electrical activity in cardiac optical mapping studies” Computers in Cardiology, Vol. 36, pp. 521-524, 2009.
[18]Hua Xiao, Xizhao Lv , “the processing of cardiac image based on optical mapping,” Biomedical Engineering and Informatics, pp.628-633, 2010.
[19]B. Valeur and M. N. Berberan-Santos, “Brief history of fluorescence and phosphorescence before the emergence of quantum theory,” Journal of Chemical Education, Vol. 88, 2011, pp.731–738.
[20]Loew, L. M., L. B. Cohen, B. M. Salzberg, A. L. Obain, and F. Bezanilla. “Charge-shift probes of membrane potential: characterization ofaminostyrylpyridinium dyes on the squid giant axon.” Biophys. J, 1985, Vol. 47, pp.71–77.
[21]Loew, L. M., L. B. Cohen, J. Dix, E. N. Fluhler, V. Montana, G. Salama,and J. Y. Wu. “A naphthyl analog of the aminostyryl pyridiniumclass of potentiometric membrane dyes shows consistent sensitivity in avariety of tissue, cell, and model membrane preparations.” J. Membr,Vol. 130, 1992 , pp.1–10.
[22]電壓敏感性螢光染劑技術資料
[23]W. Y. Kao, C. E. Davis,Y. I. Kim and J. M. Beach, “Fluorescence emission spectral shift measurements of membrane potential in single cells,” Biophysical Journal, Vol. 81, 2001, pp.1163–1170.
[24]D. Y. Tanga, Yuhua Li, “Preliminary study of a dual CCD based ratiometric optical mapping system,” Proceedings of SPIE, Vol. 4958, 2003, 1605-7422.
[25]黃信興, “頻閃白光干渉儀應用在微元件之動態分析”國立臺北科技大學自動化科技研究所研究所碩士論文, 2009.
[26]Rafael C. Gonzalez, Richard Eugene Woods, “Digital image processing,” Prentice Hall, 2008.
[27]相機技術資料http://www.mikrotron.de/
[28]蔡朝洋, “單晶片微電腦8051/8951原理與應用” 全華書局, 2003


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