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研究生:胡景皓
研究生(外文):Jing-Hao Hu
論文名稱:利用幾丁聚醣包覆磁性磷酸鈣微粒做為肺癌熱治療的熱種子
論文名稱(外文):Chitosan and Crossed Fe-DCPD Microparticles as Thermoseed for Lung Cancer Hyperthermia
指導教授:唐自標林峯輝許淙慶許淙慶引用關係
口試委員:曾靖孋張國基
口試日期:2012-07-06
學位類別:碩士
校院名稱:國立臺北科技大學
系所名稱:材料科學與工程研究所
學門:工程學門
學類:材料工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:58
中文關鍵詞:雙水磷酸氫鈣幾丁聚醣葉酸腫瘤熱治療磁滯損失霧化吸入
外文關鍵詞:DCPDChitosanFolic acidHyperthermiaHysteresis lossInhalation
相關次數:
  • 被引用被引用:2
  • 點閱點閱:138
  • 評分評分:
  • 下載下載:9
  • 收藏至我的研究室書目清單書目收藏:0
雙水磷酸氫鈣 ( CaHPO4‧2H2O ) 為無毒性、可生物降解,且具良好生物相容性之材料。本研究希望藉由摻雜二價鐵離子 ( Fe2+ ),取代雙水磷酸氫鈣中鈣離子 ( Ca2+ ) 的晶格點位置,使材料具有磁性。將此材料做為熱種子置於癌細胞表面或內部,再施予外加交流磁場,即可利用磁滯損失的現象來產生熱,使溫度上升至43~46℃,便可殺死癌細胞。
在本研究中,為了使雙水磷酸氫鈣能延長在血液中停留的時間,和方便後續接枝動作,我們利用天然高分子幾丁聚醣 ( Chitosan ) 進行表面改質,利用幾丁聚醣在中性溶液中會凝結 ( gelation ) 的特性來包覆含鐵之磁性雙水磷酸氫鈣,最後在材料表面接枝上葉酸 ( Folic Acid )。葉酸可以使磁性的雙水磷酸氫鈣更有效率的標靶到癌細胞,期望將來有可以利用霧化吸入的方式應用於肺癌熱療上。


Dicalcium phosphate dehydrate ( DCPD ) is the major component of hard tissues like bone and teeth, which is non-toxic, biocompatibility and biodegradable. In this study, the Fe-DCPD particles was formed by co-precipitation method. The iron ions can substitute calcium ions of DCPD and form a magnetic DCPD;by doing this it can release heat in alternating magnetic field because it has magnetism. As temperature rise up to 43~46℃, the heat treatment will cause cancer cells dead.
However, in order to extend the blood circulation time and convenient graft, the surface modification is necessary. Surface modification use the natural polymer chitosan. Last in modify folic acid (FA) on the magnetic particle surface, improve the targeting rate. It is believed that magnetic Fe-DCPD/Chitosan-FA is one of the most potential materials in lung cancer hyperthermia by using in halation in the future.


目錄
摘要 I
ABSTRACT II
目錄 IV
表目錄 VI
圖目錄 VII
第一章 緒論 1
1.1 前言 1
1.2 肺癌的治療方法 3
1.3 癌症熱療機制 5
1.4 熱療方式 7
1.5 磁性顆粒於癌症溫熱療法之應用 8
1.6 霧化吸入療法 10
1.6.1 利用霧化吸入療法治療肺癌的優勢 11
1.6.2 霧化吸入療法的原理 11
1.7 研究目的 13
第二章 理論基礎 14
2.1 磁性概述 14
2.1.1 磁性的來源 14
2.1.2 磁性物質分類 15
2.1.3 磁性材料的放熱機制 17
2.2 磷酸鈣陶瓷 18
2.3 雙水磷酸氫鈣 20
2.4 葉酸 ( Folic Acid, FA ) 21
2.5 幾丁聚醣 ( Chitosan ) 22
第三章 實驗材料與方法 23
3.1 實驗藥品 23
3.2 實驗儀器 24
3.3 材料製備 26
3.3.1 Fe-DCPD製備 27
3.3.2 Chitosan-FA製備 28
3.3.3 Chitosan-FA包覆Fe-DCPD 29
3.4 材料分析 30
3.4.1 X-ray繞射儀 ( XRD ) 30
3.4.2 能量散射光譜儀 ( EDS ) 30
3.4.3 傅立葉轉換紅外線光譜儀 ( FT-IR Spectrometer ) 31
3.4.4 熱分析 ( TGA/DSC ) 32
3.5 磁滯曲線與磁滯損失測量 32
3.5.1 振動樣品磁力計 ( VSM ) 32
3.5.2 超高週波加熱器 33
3.6 生物相容性測試 33
3.6.1 LDH細胞毒性測試 33
3.6.2 WST-1細胞增生測試 35
3.7 生物體外測試 ( In-Vitro ) 36
第四章 結果與討論 38
4.1 X-Ray特性分析 38
4.2 EDS成份分析 40
4.3 熱重分析 ( TGA ) 41
4.3.1 Chitosan包覆Fe-DCPD之熱重分析 41
4.3.2 Fe-DCPD/Chitosan- FA之熱重分析 42
4.4 FTIR特性分析 43
4.4.1 Chitosan包覆Fe-DCPD之IR圖譜分析 43
4.4.2 Fe-DCPD/Chitosan-FA之IR圖譜分析 45
4.5 材料磁滯損失VSM分析 46
4.6 超高週波加熱器 47
4.7 熱量吸收率 ( Spesific Power Absorption Rate, SAR ) 49
4.8 細胞毒性 LDH 測試 50
4.9 細胞增生 WST-1 測試 51
4.10 生物體外測試 In-Vitro 52
第五章 結論 55
參考文獻 56


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