跳到主要內容

臺灣博碩士論文加值系統

(44.192.22.242) 您好!臺灣時間:2021/08/03 20:22
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:許佑銓
研究生(外文):Hsu Yu-chain
論文名稱:去木質素條件與界面活性劑對蒸爆後狼尾草酵素水解之影響
論文名稱(外文):Effects of Delignification Conditions and Surfactants on The Enzymatic Hydrolysis of Steam-Exploded Napiergrass
指導教授:余琬琴
口試委員:李惠君蘇昭瑾
口試日期:2012-07-13
學位類別:碩士
校院名稱:國立臺北科技大學
系所名稱:有機高分子研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:124
中文關鍵詞:狼尾草蒸爆法去木質素界面活性劑酵素水解
外文關鍵詞:Napiergrasssteam explosiondelignificatinsurfactantenzymatic hydrolysis
相關次數:
  • 被引用被引用:1
  • 點閱點閱:259
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0

本研究所使用的狼尾草是一種深具潛力的能源作物,經由酵素水解後,再進行微生物醱酵,可將其轉換為乙醇、琥珀酸等平台化學品(Platform chemicals)。本實驗室先以蒸煮爆碎法處理後的狼尾草渣,再經由鹼性過氧化氫處理法處理,開發酵素水解及醱酵的製程。本研究的重點在於鹼性過氧化氫預處理法(Post-Treatment)的最適化,探討鹼性過氧化氫反應濃度、時間、固液比對於木質素去除率以及糖化率的影響。
研究結果顯示,兼顧醣類回收率、反應時間等因素,在雙氧水濃度2%(wt/wt),固液比1:20,反應時間30 分鐘下,木質素去除率71.76%,而葡萄糖回收率與糖化率則分別可達84.98%和90.64%
此外,界面活性劑能夠穩定酵素,增加纖維素水解成可醱酵糖的產率。因此本研究添加不同種類、不同劑量的介面活性劑於水解製程中,探討界面活性劑對於狼尾草水解之效益。界面活性劑為Tween 80時,且劑量為4.5 g/L時,葡萄糖濃度可以達最高。



Napiergrass (Pennisetum Purpureum) is regarded as apotential energy crop, the napiergrass via enzymatic hydrolysis, follow by fermentation can be converted to platform chemicals, such as ethanol, succinic acid, etc.
The residual solids after steam explosion were subjected to alkaline hydrogen peroxide treatment before enzymatic hydrolysis. The effects of alkaline hydrogen peroxide residence time and hydrogen peroxide concentration on lignin removal and enzymatic saccharification was studied.
When saccharides recovery are considered, a milder alkaline hydrogen peroxide condition should be used, The results indicate that the highest yield of saccharides was the hydrogen peroxide concentration is 2 %(wt/wt), Solid-liquid ratio is 1:20 and the residence time is 30 minute, the lignin removal and enzymatic saccharification were 71.76 % and 84.98 %.
In addition, surfactants could incrase enzyme stability and enhance the yield of cellulose to fermentationable sugars. Therefore, we add different types and different concentration of surfactants in the process of enzymatic hydrolysis to explore the effect of surfactant in the Napiergrass hydrolysis.
When the surfactant is Tween 80, and a dose of 4.5 g / L, glucose concentrations can be up to the highest.


目錄
摘 要 i
Abstract ii
第一章 緒論 1
1.1前言 1
1.2研究動機 2
1.3研究目的 3
第二章 文獻回顧 4
2.1 生質能的定義 4
2.2 生物精煉 6
2.3狼尾草 8
2.4狼尾草的組成 9
2.4.1木質纖維素 9
2.4.2 纖維素 12
2.4.3 半纖維素 14
2.4.4 木質素 15
2.5 木質纖維素前處理方法的介紹 17
2.5.1 物理性前處理 18
2.5.2 化學性前處理 18
2.5.2.1 酸水解法 18
2.5.2.2鹼處理法 19
2.5.2.3鹼性過氧化氫處理法 19
2.5.3 物理/化學性前處理 20
2.5.3.1 蒸煮爆碎法 20
2.5.3.2 濕氧蒸煮爆碎法 22
2.5.4小結 23
2.6酵素水解 24
2.6.1纖維素酵素 24
2.6.2纖維酵素水解纖維素的種類及作用方式 25
2.6.2.1內切型纖維酵素 25
2.6.2.2外切型纖維酵素 25
2.6.2.3纖維雙醣酵素 26
2.6.2.4纖維素酵素之作用方式 26
2.7影響纖維素酵素水解的因素 28
2.7.1酵素的劑量與基質的濃度對酵素水解的影響 28
2.7.2木質素對酵素水解的影響 28
2.7.3界面活性劑對酵素水解的影響 29
2.8界面活性劑 30
2.8.1界面活性劑的分類 30
2.8.2界面活性劑應用於酵素水解 31
第三章 材料與方法 33
3.1 實驗儀器 33
3.2 實驗藥品 37
3.3實驗流程 39
3.4 狼尾草來源及處理 40
3.5 標準曲線之建立 42
3.6 成分分析 42
3.6.1 乾重測試 42
3.6.2 碳水化合物分析 43
3.6.3酸不可溶木質素分析 46
3.6.4 酸可溶木質素分析 46
3.6.5 灰分分析 47
3.7溼氧蒸煮爆碎設備的操作 48
3.8 鹼浸泡處理 48
3.9鹼性過氧化氫處理 49
3.9.1 實驗設計 49
3.9.2 實驗操作 50
3.10 前處理後固體組成分析 50
3.10.1葡聚醣與木聚醣回收率 50
3.10.2木質素移除率 51
3.11 前處理後液體組成分析糖類測定(依照NREL LAP-014規範) 51
3.12酵素活性分析 52
3.12.1 cellulase活性分析 52
3.12.2 β-glucosidase活性分析 53
3.13醣化率 55
3.14 酵素水解 56
3.14.1實驗設計 56
3.14.2 酵素水解實驗步驟 57
3.15 高效能液相層析儀分析原理 58
第四章 結果與討論 60
4.1 狼尾草成分分析 60
4.2 濕氧蒸爆後水洗固體成分探討 62
4.3 鹼性過氧化氫條件對濕氧蒸爆後狼尾草渣組成之影響 63
4.3.1 鹼性過氧化氫處理後蒸爆後狼尾草渣的成份變化 64
4.3.2 鹼性過氧化氫條件對酸不可溶木質素移除率之影響 66
4.3.3 鹼性過氧化氫條件對葡聚醣回收率之影響 69
4.3.4 鹼性過氧化氫條件對糖化率之影響 72
4.3.4.1木質素移除率與糖化率的關係 74
4.3.4.2 反應時間與固液比對糖化率之影響 76
4.3.4.3過氧化氫濃度與固液比對糖化率之影響 79
4.3.5 質量平衡與葡萄糖產率之探討 82
4.4 界面活性劑對酵素水解的影響 96
4.4.1界面活性劑劑量與酵素劑量對酵素水解之影響 97
4.4.2界面活性劑種類對水解之影響 103
4.5蒸爆固體與界面活性劑對酵素水解之影響 105
4.5.1界面活性劑量與酵素劑量對酵素水解蒸爆固體之影響 106
4.5.2界面活性劑種類對酵素水解蒸爆固體之影響 112
4.5.3木質素含量對酵素水解之影響 113
第五章 結論 114
參考文獻 115



參考文獻
[1]陳凡,「二代生物質技術上路」,能源報導,第三期,2008。
[2]Gray K.A., Zhao L., Emptage M. “Bioethanol,” Current Opinion in Chemical Biology vol. 10, 2006, pp. 141-146.
[3]Mosier N., Wyman C., Dale B., Elander R., Lee Y. Y., Holtzapple, Ladisch M. M. “Features of promising technologies for pretreatment of lignocellulosic biomass,” Bioresource Technology, vol. 96, 2007, pp. 673–686.
[4]黃俊鴻,以兩階段濕氧蒸爆技術處理狼尾草同時生產木寡糖與高消化率芻料的研究。碩士論文,國立臺北科技大學有機高分子研究所。(2010)
[5]吳耿東,認識生質能源,物理雙月刊,(卅卷四期) ,2008 年八月。
[6]吳耿東,李宏台,「生質源能化腐朽為能源」,科學發展期刊,第三百八十三期,2004。
[7]泓能科技顧問(股)公司,http://blog.roodo.com/oilinsight
[8]Clarke K. G., Williams P. C., Smit M. S. and Harrison S. T. L., “Enhancement and repression of the volumetric oxygen transfer coefficient through hydrocarbon addition and its influence on oxygen transfer rate in stirred tank bioreactors,” Biochemical Engineering, vol. 28, 2006, pp. 237-242.
[9]Clark H., “The Biorefinery Concept–AnIntegrated Approach James H. Clark and Fabien E. I. Deswarte,” Green Chemistry Centre of Excellence, University of York, UK, 2007.
[10]古森本,生質能源作物之開發與潛力,農業生技產業技刊,13,pp. 46-53 (2008).
[11]成游貴,狼尾草育種與多元化利用,科學發展,407,pp. 24-29 (2006).
[12]行政院農業委員會畜產試驗所,www.tlri.gov.tw
[13]Subramaniyan S., Prema P. “Biotechnology of microbial xylanases: enzymology, molecular biology, and applicayion,” Crit. Rev. Biotechnology, vol.22, 2002, pp. 189-226.
[14]纖維乙醇之技術與文獻探討,分享‧跨越門檻與障礙,第12版 ,2007。
[15]Saha B. C., Iten L. B., Cotta M. A. and Wu Y. V., “Dilute Acid Pretreatment, Enzymatic Saccharification, and Fermentation of Rice Hulls to Ethanol,” Biotechnol. Prog., vol.21, 2005, pp. 816-822.
[16]Aiello C., Ferrer A., Ledesma A., “Effect of alkaline treatments at various temperatures on cellulase and biomass production using submerged sugarcane bagasse fermentation with Trichoderma reesei QM 9414,” Bioresour. Technol., vol.57, 1996, pp. 13-18.
[17]Sun Y. and Cheng J. Y., “Hydrolysis of lignocellulosic materials for ethanol production: a review,” Bioresour. Technol., vol.83, 2002, pp. 1-11.
[18]http://www.scidacreview.org/0905/html/biofuel.html
[19]陳文衡 郭家倫 黃文松 王嘉寶 纖維酒精技術之發展 農業生技產業季刊 2007.9.62.69
[20]Zhang Y. H. P., “Reviving the carbohydrate economy via multi-product
lignocellulose biorefineries,” Journal of Industrial Microbiology and
Biotechnology, vol.35, 2008, pp. 367-375.
[21]Swatloski R. P., Spear S. K., Holbery, J. D., Rogers R. D., “Disslution of cellulose with ionic liquids,” Journal of The American Chemical Society, vol. 124, 2002, pp. 4974-4975.
[22]Vazquez M. J., Alonso J. L., Dominguez H., Parajo J. C., “Xylooligosaccharides: manufacture and applications,” Trends in Food Science & Technology, vol.11, no.11, 2000, pp. 387-393.
[23]Cosgrove, D. J., “Cell Walls: Structures, Biogenesis, and Expansion. In: Plant Physiology,” In L.Taiz and E. Zeiger, eds. Sunderland: Sinauer Associates, Inc., 1998.
[24]Mosier N., Wyman C., Dale B., Elander R., Lee Y. Y., Holtzapple M. and Ladisch M., “Features of promising technologies for pretreatment of lignocellulosic biomass,” Bioresource Technology, vol.96, 2005, pp. 673-686.
[25]Hsu C. K., Liao J. W., Chung Y. C., Hsieh C. P. and ChanY. C., “Xylooligosaccharides and fructooligosaccharides affect the intestinal microbiota and precancerous colonic lesion development in rats, “ American Journal of Clinical Nutrition, vol.134, no.6, pp. 1523-1528.
[26]纖維乙醇之技術與文獻探討,分享‧跨越門檻與障礙,第12版 (2007)。
[27]陳嘉明,生物質木材膠合劑,台北:國立編譯館,1999。
[28]Hamelinck C. N., Van Hooijdonk G. and Faaij A. P. C., ”Ethanol from lignocellulosic biomass: Techno-economic performance in short-, middle- and long-term,” Biomass and Bioenergy, vol.28, 2005, pp. 384-410.
[29]王秀華,木材化學及其應用,台北:國立編譯館,2002。
[30]丁昭義,木材化學,台北:華香園出版社,1990
[31]Pereira Ramos L., “The chemistry involved in the steam treatment of lignocellulosic materials,” Quimica Nova, vol.26, 2003, pp. 863-871.
[32]Glazer A. W., Microbial biotechnology: fundamentals of applied microbiology, 1995.
[33]Lee R. L., Charles E. W., Tillman U. G., “Biocommodity engineering" Biotechnol. Prog.,” vol.15, 1999, pp. 777-793.


[34]Aiello C., Ferrer A., Ledesma A., “Effect of alkaline treatments at various temperatures on cellulase and biomass production using submerged sugarcane bagasse fermentation with Trichoderma reesei QM 9414,” Bioresour. Technol., vol.57, 1996, pp. 13-18.
[35]Moniruzzaman M., “Saccharification and alcohol fermentation of steam-exploded rice straw,” Bioresource Technology, vol. 55, 1996, pp.111-117.
[36]Wyman C. E., Dale B. E., Elander R. T., Holtzapple M., Ladisch M. R. and Lee Y. Y., “Comparative sugar recovery data from laboratory scale application of leading pretreatment technologies to corn stover,” Bioresource Technology, vol.96, 2005, pp. 2026-2032.
[37]李穩宏,吳大雄,「麥稈纖維酶解法產糖預處理過程工藝條件」,西北大學學報(自然科學版),1997。
[38]Esteghlalian A., Hashimoto A. G., Fenske J. J. and Penner M. H., “Modeling and optimization of the dilute-sulfuric-acid pretreatment of corn stover, poplar and switchgrass,” Bioresource Technology, vol.59, 1997, pp. 129-136.
[39]Gierer J., Holzforschung, vol.36, 1982, pp.43-51;55-64.
[40]Kratzl K., P. Claus, Lonsky W. and J. S. Gratzl, Wood Sci. Technol., vol.8, 1974, pp.35-49.
[41]Gierer, J. and F. Imsgard, Svensk Papperstid., vol.80, 1977, pp.510-518.
[42]Roberts, Jr. J.L., M.M. Morrison and D.T. Sawyer, J. Am. Chem. Soc., vol.100, 1978, pp.329-330.
[43]Spittler, T.D. and C.W. Dence, Svensk Papperstid., vol.80, 1977, pp.275-284.
[44]Kruger, H., Papier 33, No.10A, 1970, Ⅴ1-Ⅴ9.
[45]Cara C., Ruiz E., Ballesteros I., Negro M. J., Castro E., “Enhanced enzymatic hydrolysis of olive tree wood by steam explosion and alkaline peroxide delignification,” Process Biochemistry vol. 41, 2006, pp. 423–429.
[46]Cara C., Moya M., Ballesteros I., Negro M. J., Gonza′ lez A., Ruiz E., “Influence of solid loading on enzymatic hydrolysis of steam exploded or liquid hot water pretreated olive tree biomass,” Process Biochem vol. 42,2007, pp. 1003.
[47]McMillan J. D., “Pretreatment of lignocellulosic biomass,” ACS Symposium Series, vol.566, 1994, pp. 299-324.
[48]Chum H. L., Johnson D. K., Black S. K. and Overend R. P., “Pretreatment-catalyst effects and the combined severity parameter,” Applied Biochemistry and Biotechnology, vol.24-25, 1990, pp. 1-14.
[49]Mason W. H., “Process and apparatus for disintegration of wood and the like,” US Patent, vol.1, 1926, pp. 609.
[50]Mason W. H., “Apparatus for and process of explosion fibration of lignocellulose material,” US Patent, vol.1, 1928, pp. 665.
[51]Heitz M., Capek-Menard E., Keoberle P. G., Gagne J., Chornet E., Overend R. P., Taylor J. D. and Yu E., “Fractionation of Populus tremuloides at the pilot plant scale: Optimization of steam pretreatment conditions using the STAKE II technology,” Bioresource Technology, vol.35, 1991, pp. 23-32.
[52]Avellar B. K. and Glasser W. G., “Steam-assisted biomass fractionation. I. Process considerations and economic evaluation,” Biomass and Bioenergy, vol.14, 1998, pp. 205-218.
[53]Moniruzzaman M., “Saccharification and alcohol fermentation of steam-exploded rice straw,” Bioresource Technology, vol.55, 1996, pp.111-117.
[54]Sheldon J.B. Duff, Nichola Charles and Shawn D. Mansfield, “Effect of Oxygen Delignification Operating Parameters on Downstream Enzymatic Hydrolysis of Softwood Substrates,”Biotechnol. Prog., vol.19, 2003, pp.1606-1611.
[55]Carlos M., “Wet oxidation as a pretreatment method for enhancing the enzymatic convertibility of sugarcane bagasse,” Enzyme and Microbial Technology, vol.40, 2007, pp. 426-432.
[56]Fengel, D. and Wegener G., “Wood, Chemistry, Ultrastructure, Reactions. Watter de Gruyter, Berlin,” New York., 1984, pp.132-140.
[57]Duff S. J. B., Murray W. D.,“Bioconversion of forest products industry waste cellulosics to fuel ethanol:A review”, Bioresource Technology vol. 55, 1996, pp. 1.
[58]Uusitalo J. M., Nevalainen K. M. H., A. M. Harkki, J. K. C. Knowles, M. E. Penttila,“Enzyme production by recombinant Trichoderma reesei strains”, Journal of Biotechnology vol.17, 1991, pp. 35.
[59] Szengyel Z., Zacchi G., Varga A., RéczeyK.,“Cellulase production of Trichoderma reesei Rut C 30 using steam- pretreated spruce. Hydrolytic potential of cellulases on different substrates”, Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, vol. 679, 2000,pp. 84-86.
[60]齊倍慶,從堆肥中篩選纖維素分解酵素生產菌及其酵素性質研究,碩士論文,新竹國立清華大學生命科學研究所,2000。
[61]尤立智,嗜高溫纖維分解菌纖維分解酵素的探討,碩士論文,台灣大學生物
產業機電工程學研究所, 2003。
[62]戴上凱,熱穩定性纖維素分解細菌分離株之特性探討與親緣關係之研究。博士論文,國立中山大學生物科學研究所,2004。
[63]Bisaria V. S., Mishra S.,“Regulatory aspects of cellulase biosynthesis and secretion”, Critical reviews in biotechnology vol. 9, 1989, pp. 61.
[64]Bhat M. K. and S. Bhat, “Cellulose degrading enzymes and their potential industrial Applications,” Biotechnology Advances vol.15, 1997, pp. 583-620.
[65]Ghose T. K. “Cellulase biosynthesis and hydrolysis of cellulosic substances,” biochemical engineering, Vol. 6, 1976, pp.39-74.
[66]Bisaria V. S. and Ghose T. K., “Biodegradation of Cellulosic Materials - Substrates, Microorganisms, Enzymes and Products,” Enzyme and Microbial Technology, vol. 3, 1981, pp. 90-104.
[67]Béguin P, “Cloning of cellulase gene,” biotechnology vol. 6, 1987, pp. 129-162.
[68]Stenberg K., Tengborg C., Galbe M., Zacchi G., “Optimisation of steam pretreatment of SO2-impregnated mixed softwoods for ethanol production,” Chemical Technology &Biotechnology vol. 71, 1998, pp. 299-308.
[69]Cheung S. W. and Anderson B. C., “Laboratory investigation of ethanol production from municipal primary wastewater solids,” Bioresource Technology, vol. 59, 1997, pp. 81-96.
[70]Lu Y., Yang B., Gregg D., “Cellulase adsorption and an evaluation of enzyme recycle during hydrolysis of steam-exploded softwood residues,” Applied Biochemistry and Biotechnology,vol. 98-1, 2002, pp. 641-654.
[71]Berlin A., Gilkes N., Kurabi A., “Weak lignin-binding enzymes-A novel approach to improve activity of cellulases for hydrolysis of lignocellulosics,” Biochemistry and Biotechnology, vol. 121-124, 2005, pp. 163-170.

[72]Sewalt V. J. H., Glasser W. G., Beauchemin K. A., “Lignin impact on fiber degradation Reversal of inhibition of enzymatic hydrolysis by chemical modification of lignin and by additives,” Agricultural and Food Chemistry, vol. 45(5), 1997, pp. 1823-1828.
[73]Kim S., Holtzapple M.T., “Effect of structural features on enzyme digestibility of corn stover” Bioresource Technology, vol. 97(4), 2006, pp. 583-591.
[74] Eriksson T., Borjesson J., Tjerneld F., “Mechanism of surfactant effect in enzymatic hydrolysis of lignocelluloses,” Enzyme and Microbial Technology, vol. 31, 2002, pp. 353-364.
[75] Palonen H, “Role of lignin in the enzymatic hydrolysis of lignocelluloses,” Dissertation for the degree of Doctor, Finland Helsinki University of Technology, 2004.
[76]Eriksson T., Karlsson J., “A model explaining declining rate in hydrolysis of lignocellulose substrates with cellobiohydrolase I and endoglucanase I of Trichoderma reesei,” Biochemistry and Biotechnology, vol. 101(1), 2002, pp. 41-60
[77]Reese E. T., Manguire A., “Surfactants as stimulants of enzyme production by microorganisms,” Applied Microbiology, vol. 17(2), 1969, pp. 242-245.
[78]Pardo A. G., “Effect of surfactants on cellulase production by Nectria catalinensis,” Current Microbiol., vol. 33, 1996, pp.275-278.
[79]Helle S. S., Duff S. J. B., Cooper D. G. “Effect of surfactants on cellulose hydrolysis,” Biotechnol Bioeng., vol. 42, 1993, pp. 611-617.
[80]Eriksson T., Rjesson J., Tjerneld F., “Mechanism of surfactant effect in enzymatic hydrolysis of lignocelluloses,” Enzyme and Microbial Technology, vol. 31, 2002, pp.353-364.
[81]Toledano A., Serrano L., Garcia A., Mondragon I., Labidi J., “Comparative study of lignin fractionation by ultrafiltration and selective precipitation,” Chemical Engineering, vol. 157, 2010, pp. 93–99.
[82]Jonsson A. S., Nordin A.K., Wallberg O., “Concentration and purification of lignin in hardwood kraft pulping liquor by ultrafiltration and nanofiltration,” Chemical Engineering Research and Design, vol. 86, 2008, pp. 1271–1280.
[83]Mussatto S. I., Fernandes M., Roberto I. C., “Lignin recovery from brewer’s grain black liquor,” Carbohydr. Polymer, vol. 70, 2007, pp. 218–223.
[84]Garcia A., Toledano A., Serrano L., Egues I., Gonzalez M., Marin F., Labidi J., “Characterization of lignins obtained by selective precipitation, Sep,” Purif. Technol., vol.68, 2009, pp. 193–198.
[85]米孝夫原著,王鳳英編譯,界面活性劑的原理與應用,高立圖書,2007。
[86]林清安、丁幸一、林德培編著,界面活性化學,1972,pp. 7-12, 23-41。
[87]Kumar R., Wyman C. E. “Effect of Additives on the Digestibility of Corn Stover Solids Following Pretreatment by Leading Technologies,” Biotechnology and Bioengineering, vol.102(6), 2009, pp. 1544-1557.
[88]Ouyang J., Ma R., Huang W., Li X., Chen M., Yong Q., “ Enhanced saccharification of SO2 catalyzed steam-exploded corn stover by polyethylene glycol addition,” Biomass and Bioenergy vol. 35, 2011, pp. 2053-2058.
[89] Qiuzhuo Z., Weimin C., Juan W., “Stimulatory effects of biosurfactant produced by Pseudomonas aeruginosa BSZ-07 on rice straw decomposing,” Environmental Sciences vol. 20, 2008, pp. 975-980.
[90] Kristensen J. B., Rjesson J., Bruun M. H., Tjerneld F., “Use of surface active additives in enzymatic hydrolysis of wheat straw lignocelluloses,” Enzyme and Microbial Technology, vol. 40, 2007, pp. 888-895.
[91]Y. Zheng, Z. Pan, R. Zhang, D. Wang, B. Jenkins,“Non-ionic surfactants and non-catalytic protein treatment on enzymatic hydrolysis of pretreated creeping wild ryegrass”, Applied Biochemistry and Biotechnology, vol. 146, 2008, pp.231.
[92]Feng Y., Jiang J. X., Zhu L. W., Wu X. Y., “Study on the Enhancement of Cellulase Activities by Surfactant Tween 80 and Gleditsi Saponin, Chemistry and Industry of Forest Products, vol. 29, 2009, pp. 154-159.
[93]Zeng J., Ye Y., Gong D. C., Tian Y. H., Li D. Y. and Wang D., Effects of the Use of Surface Active Agents on the Hydrolysis of Cellulase, Liquor-Marking Science & Tech., Vol. 12, 2008, pp. 38-43.
[94]謝伯祥,以濕氧蒸煮爆碎前處理法提升稻殼糖化效率的研究,碩士論文,國立臺北科技大學有機高分子研究所,台北,2009。
[95]Helle S. S., Duff S. J. B., Cooper,“Effect of surfactants on cellulose hydrolysis,” Biotechnology and Bioengineering, vol. 42, 1993, pp. 611.


電子全文 電子全文(本篇電子全文限研究生所屬學校校內系統及IP範圍內開放)
連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top