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研究生:葉美君
研究生(外文):Mei-Chun Yeh
論文名稱:合成Pt-Fe-HAP磁性粒子作為癌症熱療雙重治療之研究
論文名稱(外文):To synthetize dual function Pt-Fe-HAP magnetic particles for cancer hyperthermia
指導教授:唐自標林峯輝
口試委員:張國基曾靖孋許淙慶
口試日期:2012-07-06
學位類別:碩士
校院名稱:國立臺北科技大學
系所名稱:材料及資源工程系研究所
學門:工程學門
學類:材料工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:75
中文關鍵詞:氫氧基磷灰石Cisplatin溫熱療法化學藥物療法磁性顆粒共沉法磁滯損失
外文關鍵詞:HAPCisplatinhyperthermiachemotherapymagnetic particleco-pricipitationhysteresis loss
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醫學技術日進千里,然截至2011年,癌症已經連續28年蟬聯國人十大死因之首,其死亡率更是逐年增加,因此,對於癌症治療的問題,一直都是生醫研究相當關心的方向,期望能夠降低副作用、減少病患的痛苦,以最溫和有效的方法來做治療。其中,溫熱療法是近年來相當熱門的一種治療方法,本研究藉由參雜二價鐵離子(Fe2+)與鉑離子(Pt2+),於氫氧基磷灰石(Ca10(PO4)6(OH)2)中取代鈣離子(Ca2+)的晶格點位置,藉此使材料具有磁性,並具有化療藥物Cisplatin的治療效果,以製備出複合功能之磁性氫氧基磷灰石,期能為腫瘤熱治療提供一個有益的方向。
本研究利用共沉法製備氫氧基磷灰石,於過程中添加FeCl2.4H2O和K2PtCl4以改變氫氧基磷灰石的原子組成,反應過程中溫度維持在80 °C、pH=9~9.5。接著,利用傅立葉轉換紅外線光譜儀(Fourier transform infrared rays spectrometer,FTIR)、X-ray繞射儀(X-ray diffraction,XRD)分析材料的結構與特性,以掃描式電子顯微鏡(scanning electron microscopy,SEM)、能量散射光譜儀(Energy dispersive spectrometer)、感應耦合電漿發射光譜儀(Inductively coupled plasma-optical emission spectrometer,ICP-OES)探究材料表面形貌和化學性質;最後,再以震動樣品磁度儀(Vibrating sample magnetometer,VSM)分析磁特性。
實驗結果發現,利用共沉法可成功製備出含鉑之磁性氫氧基磷灰石,其鉑含量為0.034,鐵為0.133。並且可由磁滯曲線分析圖中,證明該氫氧基磷灰石具有磁特性。爾後,以高週波電感應加熱機提供交流電感應磁場;此時,可測得材料因磁滯現象產生之熱能,顯示溫度可於10分鐘內達到理想溫度43~46°C。


Until 2011, cancer already is the top among the ten leading causes death over 28 years in Taiwan. The mortality from cancer is increasing every year. At Biomedicine field that focus on cancer therapies. Reducing side effects, decreasing pain and using effective therapies are the way that we hope for. “Thermotherapy” is the most popular one on cancer therapy. Synthesized with Fe2+ and Pt2+ and used Ca10(PO4)6(OH)2 to replace the Ca2+. By this way to let it got magnetic and have the same effect of Cisplatin. And then we got a Ca10(PO4)6(OH)2 that have composited effective.
The preparation for Ca10(PO4)6(OH)2 used Co-precipitation. During operated Co-precipitation that we add FeCl2.4H2O and K2PtCl4 to change compose of Ca10(PO4)6(OH)2 . Keeping temperature at 80 °C, and pH=9~9.5. And then used Fourier transform infrared rays spectrometer(FTIR) and X-ray diffraction(XRD) those two machine analyzed its’ composite and characteristic. Scanning electron microscopy (SEM), Energy dispersive spectrometer, and Inductively coupled plasma-optical emission spectrometer (ICP-OES) can helps us analyzed its’ surface and chemical property. Finally used Vibrating sample magnetometer (VSM) analyzed its’ magnetic characteristic.
Researching result show that it can prepare Ca10(PO4)6(OH)2 by using Co-precipitation. And the content of platinum is 0.034, and iron is 0.133. From the cart of Hystersis curve, it can prove the Ca10(PO4)6(OH)2 has magnetic. Commercial high frequency machine offered alternating current to find our magnetic. And then we can know the thermal energy from magnetic hysteresis. During 10 minutes can get the perfect temperature is 43~46°C.


摘要 I
ABSTRACT II
致謝 IV
目錄 V
表目錄 VIIII
圖目錄 IXI
第一章 緒論 1
1.1 前言 1
1.2 認識癌症 3
1.3 癌症治療方法 4
1.3.1 外科手術 5
1.3.2 放射線療法 6
1.3.3 化學藥物療法 7
1.3.4 免疫療法 8
1.3.5 溫熱療法 9
1.4 癌症之熱治療 10
1.4.1 熱療之機制 10
1.4.2 熱療之方法 12
1.5 磁性顆粒於腫瘤熱治療之應用 14
1.6 研究目的 17
第二章 理論基礎 18
2.1 磁性概述 18
2.1.1 磁性來源[31] 18
2.1.2 磁性的分類[32-34] 19
2.1.3 磁性材料的放熱機制 22
2.2 氫氧基磷灰石 24
2.2.1 氫氧基磷灰石簡介 24
2.2.2 氫氧基磷灰石之結構 26
2.2.3 氫氧基磷灰石之合成方法 27
2.2.4 元素添加對氫氧基磷灰石之影響 29
2.3 化療藥物Cisplatin 30
2.3.1 Cisplatin之簡介 31
2.3.2 Cisplatin之作用機制 32
第三章 實驗材料與方法 34
3.1 實驗藥品 34
3.2 實驗儀器 35
3.3 材料製備 37
3.4 材料分析 39
3.4.1 X-ray繞射儀(XRD) 39
3.4.2 傅立葉轉換紅外線光譜儀(FT-IR Spectrometer) 39
3.4.3 場發射掃描式電子顯微鏡及能量散佈光譜儀(SEM&EDS) 40
3.4.4 感應耦合電漿/原子發射光譜儀(ICP-AES) 40
3.5 磁滯曲線與磁滯損失之量測 41
3.5.1 振動樣品磁力計(VSM) 41
3.5.2 超高週波加熱器 41
3.6 生物相容性測試 42
3.6.1 LDH細胞毒性測試 42
3.6.2 WST-1細胞增生測試 44
3.7 生物體外測試(In-Vitro) 46
3.7.1 材料對細胞增生之影響 46
3.7.2 材料之磁性加熱測試 47
第四章 結果與討論 48
4.1 X-Ray特性分析 48
4.2 FTIR圖譜分析 50
4.3 顯微結構觀察與元素成份分析 53
4.4 ICP-AES元素成分分析 55
4.5 磁滯曲線之VSM分析 56
4.6 材料之磁滯損失升溫效果 59
4.6.1 超高週波加熱器 59
4.6.2 熱量吸收率(Spesific power absorption rate, SAR) 61
4.7 LDH細胞毒性測試 62
4.8 WST-1細胞增生測試 63
4.9 生物體外測試(In-Vitro) 64
4.9.1 Pt2+對細胞增生活性之影響 65
4.9.2 磁性加熱之測試結果 66
第五章 結論 70
參考文獻 71


1.Organization, W. H. (2005).
2.Department of Health, Executive Yuan, R.O.C. 2011 (TAIWAN).
3.R.A. Weinberg, The biology of cancer, New York: Garland Science, (2006), ISBN 0-8153-4076-1.
4.B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Molecular biology of the cell, New York: Garland Science, (2002), ISBN 0-8153-3218-1.
5.D.W. Kufe, R.E. Pollock, R.R. Weichselbaum, R.C. Bast Jr., T.S. Gansler, J.F. Holland, and E. Frei., Cancer Medicine 6, Hamilton (ON): BC Decker, (2003), ISBN 1-55009-213-8.
6.C.M. Haskell and J.S. Berek, Cancer Treatment, Philadelphia: WB Saunders, (1995), ISBN 0721649076.
7.三采文化編輯部/編,輕鬆防癌積極抗癌,台北:三采文化出版事業有限公司,(2001),第56-57頁。
8.黃世傑,癌,台北:正中書局,(1977),第50-55頁。
9.李門輝,癌的基礎科學,台北:合計圖書出版社,(1987),第 213-241頁。
10.三采文化編輯部/編,輕鬆防癌積極抗癌,台北:三采文化出版事業有限公司,(2001),第52-53頁。
11.C.M. Haskell, “Immunologic aspects of cancer chemotherapy”, Annual Review of Pharmacology and Toxicology, Vol. 17, (1977), pp. 179-195.
12.E.M. Hersh, J.U. Gutterman, and G.M. Mavligit, “Immunotherapy of human cancer”, Advances in Internal Medicine, Vol. 22, (1977), pp. 145-185.
13.G. Mathe, Cancer Active Immunotherapy, Berlin and New York: Springer-Verlag, Vol. 55, (1976), pp. 1-402.
14.李門輝,癌的基礎科學,台北:合計圖書出版社,(1987),第285-292頁。
15.S. Licht, Therapeutic heat and cold, Baltimore: Waverly Press, (1965), pp. 196.
16.J. Overgaard, History and heritage: an introduction, London: Taylor and Francis, Vol. 2, (1985), pp. 3-8.
17.F. Westermark, “Uber die behandlung des ulceration cervixcarcinoma mittels konstanter warme”, Zentralbl Gynaekol, Vol. 22, (1898), pp. 1335.
18.G. Crile, “Selective destruction of cancers after exposure to heat”, Annals of Surgery, Vol. 156, (1962), pp. 404-407, pp. 414-416.
19.C.W. Song, “Effect of local hyperthermia on blood flow and microenvironment”, Cancer Research (SUPPL.), Vol. 44, (1984), pp. 4721s-4730s.
20.王思婷,具核殼結構的磁性微脂粒之熱效應研究,碩士論文,國立成功大學化學工程學系,台南,(2008)。
21.A. Chicheł, J. Skowronek, M. Kubaszewska, M. Kanikowski, “Hyperthermia-description of a method and a review of clinical applications”, Reports of Practical Oncology & Radiotherapy, Vol. 12, Issue 5, (2007), pp. 267-275.
22.C.J. Diederich, K. Hynynen, “Ultrasound technology for hyperthermia”, Ultrasound in Medicine and Biology, Vol. 25, Issue 6, (1999), pp. 871-887.
23.M.H. Seegenschmiedt, P. Fessenden, C.C. Vernon, Thermoradiotherapy and Thermochemotherapy, Berlin: Springer, Vol. 1: Biology, Physiology, Physics, (1995), pp. 253-278.
24.W.L. Lin, Y.Y. Chen, J.Y. Yen, “Technical consideration for advanced ultrasound hyperthermia system development”, Japan Journal of Hyperthermic Oncology, Vol. 14, (1998), pp. S59-S61.
25.I. Manak, A. Lisenkova, A. Nikolaeva, “Laser hyperthermia: Problems and solutions”, Proceedings of SPIE, Vol. 5566, (2004), pp. 178-184.
26.S. Mornet, S, Vasseur, F. Grasset, E. Duguet, “Magnetic nanoparticle design for medical diagnosis and therapy”, Journal of Materials Chemistry, Issue 14, (2004), pp. 2161-2175.
27.P. Moroz, S.K. Jones, B.N. Gray, “Magnetically mediated hyperthermia: current status and future directions”, International Journal of Hyperthermia, Vol. 18, No. 4, (2002), pp. 267-284.
28.C. Plank, "Nanomagnetosols: magnetism opens up new perspectives for targeted aerosol delivery to the lung", Trends in Biotechnology, Vol. 26, Issue 2, (2008), pp. 59-63.
29.X. Wang, L. Yang, Z. Chen, D.M. Shin, “Application of Nanotechnology in Cancer Therapy and Imaging”, CA: A Cancer Journal for Clinicians, Vol. 58, Issue 2, (2008), pp. 97-110.
30.G. Kong, G. Anyarambhatla, W.P. Petros, R.D. Braun, O.M. Colvin, D. Needham, M.W. Dewhirst, “Efficacy of Liposome and Hyperthermia in a Human Tumor Xenograft Model: Importance of Triggered Drug Release”, Cancer Research, Vol. 60, (2000), pp. 6950-6957.
31.邱軍浩,Ti/C元素對PrFeB系複合奈米晶薄帶之磁性及交換藕合效應影響,博士論文,國立中正大學物理研究所,嘉義,(2006)。
32.張煦,李學養,磁性物理學,台北:聯經出版事業公司,(1982) ,第3-15頁。
33.陳繼仁,陶瓷技術手冊(上),新竹:中華民國粉末冶金學會,(1994),第561-564頁。
34.田民波,磁性材料,北京:清華大學出版社,(2000),第11-33頁。
35.江明彥,NiO/NiFe2O4奈米複合材料之合成與磁性之研究,碩士論文,南台科技大學機械工程研究所,台南,(2006)。
36.W. Andra, H. Nowak, Magnetism in Medicine: A Handbook, Berlin: Wiley-VCH, (1998), pp. 455.
37.Q.A. Pankhurst, J. Connolly, S.K. Jones, J. Dobson, “Applications of magnetic nanoparticles in biomedicine”, Journal of Physics D: Applaied Physics, Vol. 36, (2003), pp. R167-R181.
38.F. H. Albee, H. F. Morrison, "Studies in bone growth-Triple calcium phosphate as a stimulus to osteogenesis", Annals of Surgery, Vol. 71, Issue 1, (1920), pp. 32-39.
39.W.F. Dejong, “La Substance Minerale Dans les Os”, Recueil des Travaux Chimiques des Pays-Bas, Vol. 45, Issue 6, (1926), pp. 445-448.
40.C.S. Chai, B. Ben-Nissan, “Bioactive nanocrystalline sol-gel hydroxyapatite coating”, Journal of Materials Science: Materials in Medicine, Vol. 10, (1999), pp. 465-469.
41.S.D. Bruck, Properties of biomaterials in the physiological environment, Boca Raton, Florida: CRC Press, (1980), pp. 1.
42.L.L. Hench, “Bioceramics: from concept to clinic”, Journal of the American Ceramic Society, Vol. 74, Issue 7, (1991), pp. 1487-1510.
43.韓宗立,生醫用氫氧基磷灰石之研究,碩士論文,國立成功大學礦冶及材料科學研究所,台南,(1986)。
44.M.S.-A. Johnsson, G.H. Nanocollas, “The Role of Brushite and Octacalcium Phosphate in Apatite Formation”, Critical Reviews in Oral Biology and Medicine, Vol. 3, Issue 1/2, (1992), pp. 61-82.
45.J.D. Bronzino, The Biomedical Engineering Handbook, Boca Raton, Florida: CRC Press, (1995), pp. 562.
46.L. Bernard, M. Freche, J.L. Lacout, B. Biscans, “Preparation of hydroxyapatite by neutralization at low temperature—influence of purity of the raw material”, Powder Technology, Vol. 103, (1999), pp. 19-25.
47.C. Liu, Y. Huang, W. Shen, J. Cui, “Kinetics of hydroxyapatite precipitation at pH 10 to 11”, Biomaterials, Vol. 22, (2001), pp. 301-306.
48.張淑真,水熱法合成氫氧基磷灰石之研究,碩士論文,中原大學化學學系,桃園,(1993)。
49.張玉珍,製程因素對氫氧基磷灰石粉末在不同溶液析出及成長之影響,碩士論文,義守大學材料科學與工程學系,高雄,(2006)。
50.G. Willmann, “Medical grade hydroxyapatite: state of the art”, British Ceramic Transactions, Vol. 95, (1996), pp. 212-216.
51.B. Desoize, "Metals and metal compounds in cancer treatment", Anticancer Research, Vol. 24, (2004), pp. 1529-1544.
52.葉雅如,MCM-41之cisplatin釋放測試,碩士論文,國立台灣海洋大學生物科技研究所,基隆,(2006)。
53.R.T. Skeel, S. Khleif, Handbook of Cancer Chemotherapy, Taiwan: Lippincott Williams & Wilkins, (1999).
54.K. Stehlikova, H. Kostrhunova, J. Kasparkova, V. Brabec, “DNA bending and unwinding due to the major 1,2-GG intrastrand cross-link formed by antitumor cis-diamminedichloroplatinum(II) are flanking-base independent”, Nucleic Acids Research, Vol. 30, No. 13, (2002), pp. 2894-2898.
55.D.B. Zamble, S.J. Lippard, “Cisplatin and DNA Repair in Cancer Chemotherapy”, Trends in Biochemical Sciences, Vol. 20, Issue 10, (1995), pp. 435-439.
56.E.R. Jamieson, S.J. Lippard, “Structure, Recognition, and Processing of Cisplatin−DNA Adducts”, Chemical Reviews, Vol. 99, No. 9, (1999), pp. 2467-2498.
57.M. Kartalou, J.M. Essigmann, “Mechanisms of Resistance to Cisplatin”, Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 478, Issue 1-2, (2001), pp. 23-43.
58.D. Wang, S.J. Lippard, “Cellular Processing of Platinum Anticancer Drugs”, Nature Reviews Drug Discovery 4, Vol. 4, (2005), pp. 307-320.
59.Bio Vision, LDH-Cytotoxicity Assay Kit, Montain View: Bio Vision Research Products, Catalog#K311-400.
60.Takara, Premix WST-1 Cell Proliferation Assay System, Takara, Catalog ﹟MK400.
61.C.C. Silva, D. Thomazini, A.G. Pinheiro, F. Lanciotti Jr., J.M. Sasaki, J.C. Goes, A.S.B. Sombra, “Optical Properties of Hydroxyapatite Obtained by Mechanical Alloying”, Journal of Physics and Chemistry of Solids, Vol. 63, Issue 9, (2002), pp. 1745-1757.
62.K. Kandori, N. Horigami, A. Yasukawa, T. Ishikawa, “Texture and Formation Mechanism of Fibrous Calcium Hydroxyapatite Particles Prepared by Decomposition of Calcium–EDTA Chelates”, Journal of the American Ceramic Society, Vol. 80, Issue 5, (1997), pp. 1157-1164.
63.L.L. Hench, J. Wilson, An Introduction to Bioceramics, Singapore: World Scientific, (1993).


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12. 陳毓文、鄭玲惠(2000)。為什麼是我?胰島素依賴型糖尿病少年生活經驗之初探。中華心理衛生學刊,13(1),1-26。
13. 陳毓文、鄭玲惠(2000)。為什麼是我?胰島素依賴型糖尿病少年生活經驗之初探。中華心理衛生學刊,13(1),1-26。
14. 陳毓文、鄭玲惠(2000)。為什麼是我?胰島素依賴型糖尿病少年生活經驗之初探。中華心理衛生學刊,13(1),1-26。
15. 陳明蕾(1999)。一個新的學習典範:觀點轉化學習理論。成人教育學刊,3,1-32。