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研究生:林欣嵐
研究生(外文):Sin-Lan Lin
論文名稱:靈芝蛋白LZ-8促進傷口癒合研究
論文名稱(外文):Accelerated skin wound healing with fungal immunomodulatory protein, Ling Zhi-8
指導教授:張榮善
指導教授(外文):Jungshan Chang
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:醫學科學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:英文
論文頁數:45
中文關鍵詞:靈芝蛋白 傷口癒合
外文關鍵詞:LZ-8 wound healing skin
相關次數:
  • 被引用被引用:0
  • 點閱點閱:251
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  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:1
ABSTRACT 1
摘要 3
ABBREVIATIONS 4
INTRODUCTION 5
MATERIALS AND METHODS 14
Treatment of LZ-8 14
Animal model 14
Cell culture 15
Cell count 15
Wound healing assay 16
Cell proliferation assay 17
Histologic analysis 18
ToF-SIMS analysis 18
Statistical analysis 19
RESULTS 20
DISCUSSION 32
CONCLUSIONS 35
ACKNOWLEDGMENTS 36
REFERENCES 37

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2.van der Hem, L.G., van der Vliet, J.A., Kino, K., Hoitsma, A.J. & Tax, W.J. Ling-Zhi-8: a fungal protein with immunomodulatory effects. Transplant Proc 28, 958-959 (1996).

3.Tanaka, S., et al. Complete amino acid sequence of an immunomodulatory protein, ling zhi-8 (LZ-8). An immunomodulator from a fungus, Ganoderma lucidium, having similarity to immunoglobulin variable regions. J Biol Chem 264, 16372-16377 (1989).

4.Hsu, H.Y., et al. Reishi immuno-modulation protein induces interleukin-2 expression via protein kinase-dependent signaling pathways within human T cells. J Cell Physiol 215, 15-26 (2008).

5.Lin, Y.L., et al. An immunomodulatory protein, Ling Zhi-8, induced activation and maturation of human monocyte-derived dendritic cells by the NF-kappaB and MAPK pathways. J Leukoc Biol 86, 877-889 (2009).

6.Yeh, C.H., Chen, H.C., Yang, J.J., Chuang, W.I. & Sheu, F. Polysaccharides PS-G and protein LZ-8 from Reishi (Ganoderma lucidum) exhibit diverse functions in regulating murine macrophages and T lymphocytes. J Agric Food Chem 58, 8535-8544 (2010).

7.Haak-Frendscho, M., Kino, K., Sone, T. & Jardieu, P. Ling Zhi-8: a novel T cell mitogen induces cytokine production and upregulation of ICAM-1 expression. Cell Immunol 150, 101-113 (1993).


8.Miyasaka, N., et al. An immunomodulatory protein, Ling Zhi-8, facilitates cellular interaction through modulation of adhesion molecules. Biochem Biophys Res Commun 186, 385-390 (1992).

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10.Kino, K., et al. An immunomodulating protein, Ling Zhi-8 (LZ-8) prevents insulitis in non-obese diabetic mice. Diabetologia 33, 713-718 (1990).

11.Wu, C.T., et al. Ling Zhi-8 mediates p53-dependent growth arrest of lung cancer cells proliferation via the ribosomal protein S7-MDM2-p53 pathway. Carcinogenesis 32, 1890-1896 (2011).

12.Lin, C.C., et al. A novel adjuvant Ling Zhi-8 enhances the efficacy of DNA cancer vaccine by activating dendritic cells. Cancer Immunol Immunother 60, 1019-1027 (2011).

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30.Theilgaard-Monch, K., Knudsen, S., Follin, P. & Borregaard, N. The transcriptional activation program of human neutrophils in skin lesions supports their important role in wound healing. J Immunol 172, 7684-7693 (2004).

31.Canturk, N.Z., et al. The relationship between neutrophils and incisional wound healing. Skin Pharmacol Appl Skin Physiol 14, 108-116 (2001).

32.Dovi, J.V., He, L.K. & DiPietro, L.A. Accelerated wound closure in neutrophil-depleted mice. J Leukoc Biol 73, 448-455 (2003).

33.Dovi, J.V., Szpaderska, A.M. & DiPietro, L.A. Neutrophil function in the healing wound: adding insult to injury? Thromb Haemost 92, 275-280 (2004).

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35.Resing, K.A., al-Alawi, N., Blomquist, C., Fleckman, P. & Dale, B.A. Independent regulation of two cytoplasmic processing stages of the intermediate filament-associated protein filaggrin and role of Ca2+ in the second stage. J Biol Chem 268, 25139-25145 (1993).

36.Grzesiak, J.J. & Pierschbacher, M.D. Shifts in the concentrations of magnesium and calcium in early porcine and rat wound fluids activate the cell migratory response. J Clin Invest 95, 227-233 (1995).


37.Thiel, V. & Sjovall, P. Using time-of-flight secondary ion mass spectrometry to study biomarkers. Vol. 39 125-156 (2011).

38.Yeh, C.M., Yeh, C.K., Hsu, X.Y., Luo, Q.M. & Lin, M.Y. Extracellular expression of a functional recombinant Ganoderma lucidium immunomodulatory protein by Bacillus subtilis and Lactococcus lactis. Appl Environ Microbiol 74, 1039-1049 (2008).

39.Lin, Q., et al. Impaired wound healing with defective expression of chemokines and recruitment of myeloid cells in TLR3-deficient mice. J Immunol 186, 3710-3717 (2011).

40.Larson, K., Ho, H.H., Anumolu, P.L. & Chen, T.M. Hematoxylin and eosin tissue stain in Mohs micrographic surgery: a review. Dermatol Surg 37, 1089-1099 (2011).

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46.Straino, S., et al. High-mobility group box 1 protein in human and murine skin: involvement in wound healing. J Invest Dermatol 128, 1545-1553 (2008).

47.Ohgoda, O., et al. Fibroblast-migration in a wound model of ascorbic acid-supplemented three-dimensional culture system: the effects of cytokines and malotilate, a new wound healing stimulant, on cell-migration. J Dermatol Sci 17, 123-131 (1998).

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