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研究生:乃柏齡
研究生(外文):Po-Ling Nai
論文名稱:CCAAT/ Enhancer Binding Protein β 訊息路徑參與Thrombin誘導肺部上皮細胞IL-8/CXCL8表現之探討
論文名稱(外文):Signal pathway of CCAAT/Enhancer Binding Protein β mediates Thrombin-Induced IL-8/CXCL8 Expression in Human Lung Epithelial Cells
指導教授:林建煌林建煌引用關係
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:醫學科學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:89
中文關鍵詞:c/EBP betaIL-8/CXCL8thrombin
外文關鍵詞:c/EBP betaIL-8/CXCL8thrombin
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壹、中文摘要 1
貳、英文摘要 4
参、緒論 7
1. 氣喘及呼吸道發炎反應 8
2. 肺部上皮細胞的功能 8
3. Thrombin 和肺部疾病的關係 9
4. IL-8/CXCL8功能及基因轉路調控機制 11
5. c/EBP家族及活化訊息傳遞路徑 12
6. MEKK及RSK的角色 14
肆、實驗材料與方法 17
一、實驗材料 18
二、方法 18
1.細胞培養 19
2.細胞蛋白質淬取 19
3.西方墨點法 20
4. IL-8/CXCL8 ELISA測定 21
5.轉染及報告基因試驗 21
6.染色質免疫沈澱試驗 22
7.統計方法 23
伍、結果 24
1. Thrombin經由c/EBPβ誘導肺部上皮細胞IL-8/CXCL8的表現及釋放 25
2. Thrombin誘導A549細胞c/EBPβ的活化 26
3. Thrombin經由活化RSK1誘導肺部上皮細胞IL-8/CXCL8的表現 27
4. Thrombin經由活化RSK1誘導肺部上皮細胞c/EBPβ的表現 27
5. Thrombin經由活化ERK誘導肺部上皮細胞IL-8/CXCL8的釋放 28
6. Thrombin經由活化ERK誘導肺部上皮細胞RSK1及c/EBPβ的活化 29
7. Thrombin經由MELK1誘導肺部上皮細胞IL-8/CXCL8的表現 30
8. Thrombin經由活化MEEK1誘導肺部上皮細胞RSK1,ERK及c/EBPβ的活化 31
9. Thrombin誘導c/EBPβ結合於 IL-8/CXCL8 promoter 32
陸、討論 34
未來展望 41
柒、參考文獻 42
捌、圖表 54
玖、附圖 74
附圖一 75
附圖二 76
附圖三 76
附圖四 77


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