臺灣博碩士論文加值系統

本研究首先針對碳源(葡萄糖, glucose)的濃度(10-50 g/L)進行探討。研究結果指出，以50 g/L為碳源發酵時，可以得到0.43 g/L丁醇。
之後便並進一步探討不同碳源—甘油(glycerol)對於丁醇生產的效果，結果發現確實有提升丁醇產量之功用。
本研究進一步探討氮源(yeast extract, tryptone, peptone)對生產丁醇的影響，研究結果發現，培養基同時以添加三種氮源做為微生物培養基，其丁醇的產量較個別單一氮源的產量最高可達到1.5倍，顯示此三種氮源的添加有其必要性，對菌體的生長尤其重要。
除碳氮、源外，本研究同時針對trace elements(Fe、Mg)進行探討。研究結果發現，不同FeSO4．7(H2O)的濃度對微生物的生長並無影響，但很明顯的0.0025 g/L FeSO4．7(H2O)可產生0.7 g/L的丁醇；另外，發現不同濃度的MgSO4．7(H2O)對菌體是有影響的，從實驗結果來看，會發現菌體生長隨著MgSO4．7(H2O)濃度越高，在同個時間內菌體值越高，此外，亦發現丁醇濃度在0.05 g/L MgSO4．7(H2O)下可達到0.8 g/L，其餘丁醇濃度皆在0.4 g/L，由此實驗可知MgSO4．7(H2O)對丁醇產量有幫助，但是濃度不能過高(0.2 g/L)，也不能過低(0.0192 g/L)，否則會影響丁醇生產。

In these studies, we investigated the effect of carbon source (glucose) with different concentrations (10-50 g/L) associated with Clostridium acetobutylicum ATCC 824 growth and butanol production. These results suggest that it produced 0.43 g/L butanol in 60 h with 50 g/L glucose as carbon source. Using medium containing glycerol, instead of glucose as a carbohydrate source, we measured characteristics of the fermentation
including solvent production. With glycerol/glucose as substrate, the culture produced 1.6 g /L butanol.
When yeast extract, tryptone and peptone were introduced into the fermentation medium, growth and ABE production by C.acetobutylicum ATCC 824 increased significantly. Besides that, medium supplemented with cysteine-HCl promote bacterial growth, but it doesn't affect the butanol production and by adding glutathione to the growth medium bio-butanol production can be increased from 0.2 g/L to 0.4 g/L. Finally, the addition of 0.025 g/L traces elements (FeSO4．7(H2O)) can also improve the yield of bio-butanol to 0.7 g/L.

目錄
誌謝 vi
中文摘要 vii
英文摘要 ix
目錄 x
圖目錄 xiii
表目錄 xiv
第一章 緒論 1
1.1 簡介 1
1.2 研究動機 2
1.3 論文架構 3
第二章 文獻回顧 4
2.1 生質能源簡介 4
2.2 丁醇介紹 5
2.3 菌種介紹 6
2.4 微生物利用葡萄糖之代謝路徑 8
第三章 材料與方法
3.1 實驗材料 10
3.2 分析方法 11
3.3 實驗方法 18
3.4 實驗培養條件 20
第四章 結果與討論
4.1.1.1 本實驗探討不同葡萄糖濃度之影響 23
4.1.1.2 不同碳源對於菌體和丁醇之影響
28
4.1.1.3 不同氮源對生產丁醇之影響
33
4.1.1.4 微量元素對於產物丁醇之影響 38
4.1.1.5胺基酸對菌體之影響 47
4.1.2.1不同溫度對菌體之影響 51
4.1.2.2初始pH值的調控 55
4.1.3.1加入丁醇誘導產量提高 59
4.1.3.2發酵槽培養條件測試 63
第五章 結論與未來展望 65
參考文獻 67

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