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研究生:黃靖樺
研究生(外文):Hua-Ching Huang
論文名稱:探討細胞自噬作用在第二型和第三型小腦脊髓運動失調症中所扮演的角色
論文名稱(外文):The Role of Autophagy in Pathogenesis of Spinalcerebellar Ataxia Type 2 and Type 3
指導教授:蔡蕙芳蔡蕙芳引用關係
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:醫學檢驗暨生物技術學系碩士班
學門:醫藥衛生學門
學類:醫學技術及檢驗學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:65
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中文摘要 i
Abstrate iii
目錄 iv
圖表目錄 vi
壹、緒論 1
一、小腦脊髓運動失調症(Spinocerebellar Ataxia, SCA) 1
二、小腦脊髓運動失調症第三型(Spinocerebellar ataxia type 3;SCA3) 2
三、小腦脊髓運動失調症第二型(Spinocerebellar ataxia type 2;SCA2) 4
四、細胞自噬(Autophagy) 5
五、細胞自噬對神經退化性疾病的影響 7
六、氧化壓力對神經退化性疾病的影響 9
貳、材料與方法 11
一、細胞培養(Cell culture) 11
二、細胞生長速率分析(Cell viability) 11
三、細胞飢餓(Cell Starvation) 12
四、DNA純化(DNA purification) 12
五、聚合酶鏈鎖反應(Polymerase chain reaction; PCR) 13
六、蛋白純化(Protein purification) 13
七、西方點墨法(Western blot) 14
八、細胞週期(Cell Cycle) 15
九、酸性囊狀胞器分析(Acridine Orange stain) 15
十、細胞內粒線體膜電位(Mitochondrial Membrane Potential; MMP) 16
十一、細胞內活性氧化物(Reactive Oxygen Species; ROS) 16
十二、統計分析 17
參、結果 19
一、第三型小腦萎縮症MJD07、MJD08細胞株之異常CAG擴增 19
二、LCL、MJD07及MJD08細胞自噬差異性分析 19
三、分析LCL、MJD07及MJD08細胞株生長週期及粒線體膜電位 20
四、MJD07、MJD08細胞株有較強活性氧化物(ROS)表現 21
五、LCL、MJD07和MJD08細胞自噬機制誘導 21
六、Rapamycin誘導細胞自噬作用及細胞生長情形 22
七、Bafilomycin A1誘導細胞自噬作用及細胞生長情形 23
八、比較LCL、SCA2細胞株CAG擴增差異 24
九、分析LCL及SCA2細胞株生長情形及粒線體膜電位 24
十、分析LCL及SCA2細胞株於飢餓狀態下其細胞週期表現 24
十一、分析LCL及SCA2細胞株細胞自噬表現 25
十二、熱休克蛋白在正常細胞株及SCA2細胞株之表現情形 25
十三、分析LCL及SCA2經rapamycin誘導細胞自噬作用及細胞株生長 26
十四、觀察bafilomycin A1誘導細胞自噬作用及細胞株生長情形於LCL、SCA2 26
肆、討論 28
伍、參考文獻 33
陸、圖表與圖表說明 47
柒、附錄 64
附錄一、第三型小腦脊髓運動失調症細胞株之Ataxin-3蛋白表現情形 64
附錄二、細胞自噬機制表現圖 65



Aguiar, J., Fernandez J., et al. Ubiquitous expression of human SCA2 gene under the regulation of the SCA2 self promoter cause specific Purkinje cell degeneration in transgenic mice.Neurosci Lett 392 (3): 202-206 , (2006).

Alonso I., Costa C., et al. A novel H101Q mutation causes PKCgamma loss in spinocerebellar ataxia type 14. J Hum Genet 50 (10): 523-529, (2005).

Andres, A. M., Lao, O., et al. Dynamics of CAG repeat loci revealed by the analysis of their variability. Hum Mutat 21 (1): 61-70, (2003).

Andrew, S. E., Goldberg, Y. P., et al. The relationship between trinucleotide (CAG) repeat length and clinical features of Huntington''s disease.Nat Genet 4 (4): 398-403, (1993).

Araujo, J., P. Breuer, et al. FOXO4-dependent upregulation of superoxide dismutase-2 in response to oxidative stress is impaired in spinocerebellar ataxia type 3. Hum Mol Genet 20 (15): 2928-2941, (2011).

Arduino, D. M., Esteves, A. R., et al. Mitochondrial fusion/fission, transport and autophagy in Parkinson''s disease: when mitochondria get nasty. Parkinsons Dis 2011: 767230, (2011).

Arrasate, M., Mitra, S., et al. Inclusion body formation reduces levels of mutant huntingtin and the risk of neuronal death.Nature 431 (7010): 805-810, (2004).

Basso, M., Pozzi, S., et al. Mutant copper-zinc superoxide dismutase (SOD1) induces protein secretion pathway alterations and exosome release in astrocytes: implications for disease spreading and motor neuron pathology in amyotrophic lateral sclerosis. J Biol Chem 288 (22) : 15699- 711, (2013).

Bento, C. F., Puri, C., et al. The role of membrane-trafficking small GTPases in the regulation of autophagy. J Cell Sci 126 (Pt 5): 1059-1069, (2013).

Bettencourt, C. and Lima M. Machado-Joseph Disease: from first descriptions to new perspectives.Orphanet J Rare Dis (6): 35, (2011).

Boeddrich, A., Gaumer, S., et al. An arginine/lysine-rich motif is crucial for VCP/p97-mediated modulation of ataxin-3 fibrillogenesis. EMBO J 25 (7): 1547-1558, (2006).

Boje, K. M. and Arora, P. K. Microglial-produced nitric oxide and reactive nitrogen oxides mediate neuronal cell death. Brain Res 587 (2): 250-256, (1992).

Brusco, A., Gellera, C., et al. Molecular genetics of hereditary spinocerebellar ataxia: mutation analysis of spinocerebellar ataxia genes and CAG/CTG repeat expansion detection in 225 Italian families.Arch Neurol 61 (5): 727-733, (2004).

Cemal, C. K., Carroll, C. J., et al. YAC transgenic mice carrying pathological alleles of the MJD1 locus exhibit a mild and slowly progressive cerebellar deficit.Hum Mol Genet 11 (9) : 1075-1094, (2002).
Ciosk, R., DePalma, M., et al. ATX-2, the C. elegans ortholog of ataxin 2, functions in translational regulation in the germline.Development 131 (19): 4831-4841, (2004).

David, G., Abbas, N., et al. Cloning of the SCA7 gene reveals a highly unstable CAG repeat expansion.Nat Genet 17 (1): 65-70, (1997).

Ding, W. X., Manley, S., et al. The emerging role of autophagy in alcoholic liver disease.Exp Biol Med (Maywood) 236 (5) : 546-556, (2011).

Evers, M. M., Tran, H. D., et al. Ataxin-3 protein modification as a treatment strategy for Spinocerebellar Ataxia type 3: Removal of the CAG containing exon.Neurobiol Dis 58C:49-56, (2013).

Filomeni, G., Graziani, I., et al. Neuroprotection of kaempferol by autophagy in models of rotenone-mediated acute toxicity: possible implications for Parkinson''s disease. Neurobiol Aging 33 (4): 767-785, (2012).

Giuliani, C. M. and C. R. Dass Autophagy and cancer: taking the ''toxic'' out of cytotoxics.J Pharm Pharmacol 65 (6): 777-789, (2013).

Gotoh, H., Takahashi, T., et al. Dissection of the superior cerebellar artery: a report of two cases and review of the literature.J Clin Neurosci 11 (2): 196-199, (2004).

Holmes, S. E., O''Hearn, E. E., et al. Expansion of a novel CAG trinucleotide repeat in the 5'' region of PPP2R2B is associated with SCA12.Nat Genet 23 (4) : 391-392, (1999).

Huynh, D. P., Figueroa, K. et al. Nuclear localization or inclusion body formation of ataxin-2 are not necessary for SCA2 pathogenesis in mouse or human. Nat Genet 26 (1) : 44-50, (2000).

Ikeda, Y., Dick K. A., et al. Spectrin mutations cause spinocerebellar ataxia type 5.Nat Genet 38 (2) : 184-190, (2006).

Imbert, G., Saudou, F. et al. Cloning of the gene for spinocerebellar ataxia 2 reveals a locus with high sensitivity to expanded CAG/glutamine repeats. Nat Genet 14 (3) : 285-291, (1996).

Jaeger, P. A. and Wyss-Coray, T. All-you-can-eat: autophagy in neurodegeneration and neuroprotection. Mol Neurodegener 4: 16, (2009).

Jiang, H., Tang, B., et al. Spinocerebellar ataxia type 6 in Mainland China: molecular and clinical features in four families.J Neurol Sci 236(1-2): 25-29, (2005).

Kawaguchi, Y., Okamoto, T., et al. CAG expansions in a novel gene for Machado-Joseph disease at chromosome 14q32.1.Nat Genet 8 (3): 221-228, (1994).

Krick, R., Muhe, Y., et al. Piecemeal microautophagy of the nucleus: genetic and morphological traits. Autophagy 5 (2) : 270-272, (2009).

Kroemer, G. and White, E., et al. Autophagy for the avoidance of degenerative, inflammatory, infectious, and neoplastic disease.Curr Opin Cell Biol 22 (2) : 121-123, (2010).

Kvam, E. and Goldfarb D. S. Nucleus-vacuole junctions and piecemeal microautophagy of the nucleus in S. cerevisiae.Autophagy 3 (2) : 85-92, (2007).

Lastres-Becker, I., Rub, U., et al. Spinocerebellar ataxia 2 (SCA2). Cerebellum 7 (2) : 115-124, (2008).

Lebovitz, C. B., Bortnik, S. B., et al. Here, there be dragons: charting autophagy-related alterations in human tumors. Clin Cancer Res 18 (5) : 1214-1226, (2012).

Lu, J. H., Tan, J. Q., et al. Isorhynchophylline, a natural alkaloid, promotes the degradation of alpha-synuclein in neuronal cells via inducing autophagy. Autophagy 8 (1) : 98-108, (2012).

Luc, R. and Vergely, C. Forgotten radicals in biology. Int J Biomed Sci 4 (4) : 255-259, (2008).

Maciel, P., Gaspar, C., et al. Correlation between CAG repeat length and clinical features in Machado-Joseph disease. Am J Hum Genet 57 (1) : 54-61, (1995).

Maiuri, M. C., Zalckvar, E., et al. Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat Rev Mol Cell Biol 8 (9) : 741-752, (2007).
Majeski, A. E. and Dice, J. F. Mechanisms of chaperone-mediated autophagy. Int J Biochem Cell Biol 36 (12) : 2435-2444, (2004).

Mao, R., Aylsworth, A. S., et al. Childhood-onset ataxia: testing for large CAG-repeats in SCA2 and SCA7. Am J Med Genet 110 (4) : 338-345, (2002).

Markaki, M. and Tavernarakis, N. Metabolic Control by Target of Rapamycin and Autophagy during Ageing - A Mini-Review. Gerontology. 59 (4) : 340-348, (2013).

Maruyama, H., Nakamura, S., et al. Molecular features of the CAG repeats and clinical manifestation of Machado-Joseph disease. Hum Mol Genet 4 (5) : 807-812, (1995).

McCoy, M. K. and Cookson, M. R. Mitochondrial quality control and dynamics in Parkinson''s disease. Antioxid Redox Signal 16 (9) : 869-882, (2012).

Melendez, A. and Levine, B. Autophagy in C. elegans. WormBook: 1-26, (2009).

Menzies, F. M., Huebener, J., et al. Autophagy induction reduces mutant ataxin-3 levels and toxicity in a mouse model of spinocerebellar ataxia type 3. Brain 133 (Pt 1) : 93-104, (2010).

Mitsui, K., Nakayama, H., et al. Purification of polyglutamine aggregates and identification of elongation factor-1alpha and heat shock protein 84 as aggregate-interacting proteins. J Neurosci 22 (21) : 9267-9277, (2002).

Moseley, M. L., Benzow, K. A., et al. Incidence of dominant spinocerebellar and Friedreich triplet repeats among 361 ataxia families.
Neurology 51 (6) : 1666-1671, (1998).

Nakamura, K., Jeong, S. Y., et al. SCA17, a novel autosomal dominant cerebellar ataxia caused by an expanded polyglutamine in TATA-binding protein. Hum Mol Genet 10 (14) : 1441-1448, (2001).

Nascimento-Ferreira, I., Santos-Ferreira, T., et al. Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease. Brain 134 (Pt 5) : 1400-1415, (2011).

Ng, H., Pulst, S. M., et al. Ataxin-2 mediated cell death is dependent on domains downstream of the polyQ repeat. Exp Neurol 208 (2) : 207-215, (2007).

Nikali, K., Isosomppi, J., et al. Toward cloning of a novel ataxia gene: refined assignment and physical map of the IOSCA locus (SCA8) on 10q24. Genomics 39 (2) : 185-191, (1997).

Nobrega, C., Nascimento-Ferreira, I., et al. Overexpression of Mutant Ataxin-3 in Mouse Cerebellum Induces Ataxia and Cerebellar Neuropathology. Cerebellum, (2012).

Nobrega, C., Nascimento-Ferreira, I., et al. Silencing mutant ataxin-3 rescues motor deficits and neuropathology in Machado-Joseph disease transgenic mice. PLoS One 8 (1) : e52396, (2013).

Perucho, J., Casarejos, M. J., et al. Trehalose protects from aggravation of amyloid pathology induced by isoflurane anesthesia in APP(swe) mutant mice. Curr Alzheimer Res 9 (3) : 334-343, (2012).

Pivtoraiko, V. N., Harrington, A., J. et al. Low-dose bafilomycin attenuates neuronal cell death associated with autophagy-lysosome pathway dysfunction. J Neurochem 114 (4) : 1193-1204, (2010).

Pulst, S. M., Nechiporuk, A., et al. Moderate expansion of a normally biallelic trinucleotide repeat in spinocerebellar ataxia type 2. Nat Genet 14 (3) : 269-276, (1996).

Ralser, M., Albrecht, M., et al. An integrative approach to gain insights into the cellular function of human ataxin-2. J Mol Biol 346 (1) : 203-214, (2005).

Ranum, L. P., Chung, M. Y., et al. Molecular and clinical correlations in spinocerebellar ataxia type I: evidence for familial effects on the age at onset. Am J Hum Genet 55 (2) : 244-252, (1994).

Riess, O., Rub, U., et al. SCA3: neurological features, pathogenesis and animal models. Cerebellum 7 (2) : 125-137, (2008).

Rodriguez-Enriquez, S., Kim, I., et al. Tracker dyes to probe mitochondrial autophagy (mitophagy) in rat hepatocytes. Autophagy 2 (1) : 39-46, (2006).

Rub, U., Burk, K., et al. Damage to the reticulotegmental nucleus of the pons in spinocerebellar ataxia type 1, 2, and 3. Neurology 63 (7) : 1258-1263, (2004).

Sachdev, H. S., Puri, M. P., et al. Acute cerebellar ataxia in typhoid fever. Indian Pediatr 19 (7) : 639-640, (1982).

Saleem, Q., Choudhry, S., et al. Molecular analysis of autosomal dominant hereditary ataxias in the Indian population: high frequency of SCA2 and evidence for a common founder mutation. Hum Genet 106 (2) : 179-187, (2000).

Sanpei, K., Takano, H., et al. Identification of the spinocerebellar ataxia type 2 gene using a direct identification of repeat expansion and cloning technique, DIRECT. Nat Genet 14 (3) : 277-284, (1996).

Satterfield, T. F., Jackson, S. M., et al. A Drosophila homolog of the polyglutamine disease gene SCA2 is a dosage-sensitive regulator of actin filament formation. Genetics 162 (4) : 1687-1702, (2002).

Schmidt, T., Lindenberg, K. S., et al. Protein surveillance machinery in brains with spinocerebellar ataxia type 3: redistribution and differential recruitment of 26S proteasome subunits and chaperones to neuronal intranuclear inclusions. Ann Neurol 51 (3) : 302-310, (2002).

Schols, L., Gispert, S., et al. Spinocerebellar ataxia type 2. Genotype and phenotype in German kindreds. Arch Neurol 54 (9) : 1073-1080, (1997).

Shacka, J. J., Klocke, B. J., et al. Bafilomycin A1 inhibits chloroquine-induced death of cerebellar granule neurons. Mol Pharmacol 69 (4) : 1125-1136, (2006).

Sorolla, M. A., Reverter-Branchat, G.,et al. Proteomic and oxidative stress analysis in human brain samples of Huntington disease. Free Radic Biol Med 45 (5) : 667-678, (2008).

Steele, J. W., Ju, S., et al. Latrepirdine stimulates autophagy and reduces accumulation of alpha-synuclein in cells and in mouse brain. Mol Psychiatry, (2012).

Storey, E., du Sart, D., et al. Frequency of spinocerebellar ataxia types 1, 2, 3, 6, and 7 in Australian patients with spinocerebellar ataxia. Am J Med Genet 95 (4) : 351-357, (2000).

Swisher, K. D. and Parker, R. Localization to, and effects of Pbp1, Pbp4, Lsm12, Dhh1, and Pab1 on stress granules in Saccharomyces cerevisiae. PLoS One 5 (4) : e10006, (2010).

Takiyama, Y., Igarashi, S., et al. Evidence for inter-generational instability in the CAG repeat in the MJD1 gene and for conserved haplotypes at flanking markers amongst Japanese and Caucasian subjects with Machado-Joseph disease. Hum Mol Genet 4 (7) : 1137-1146, (1995).

Takiyama, Y., Nishizawa, M., et al. The gene for Machado-Joseph disease maps to human chromosome 14q." Nat Genet 4 (3) : 300-304, (1993).

Teive, H. A. Spinocerebellar ataxias." Arq Neuropsiquiatr 67 (4) : 1133-1142, (2009).

Tharun, S. Roles of eukaryotic Lsm proteins in the regulation of mRNA function. Int Rev Cell Mol Biol 272: 149-189, (2009).

Trottier, Y., Lutz, Y., et al. Polyglutamine expansion as a pathological epitope in Huntington''s disease and four dominant cerebellar ataxias. Nature 378 (6555) : 403-406, (1995).

Trushina, E. and McMurray, C. T. Oxidative stress and mitochondrial dysfunction in neurodegenerative diseases. Neuroscience 145 (4) : 1233-1248, (2007).

Tsai, H. F., Liu, C. S., et al. Prenatal diagnosis of Machado-Joseph disease/Spinocerebellar Ataxia Type 3 in Taiwan: early detection of expanded ataxin-3. J Clin Lab Anal 17 (5) : 195-200, (2003).

van Duijn, de Knijff, C. M., P., et al. Apolipoprotein E4 allele in a population-based study of early-onset Alzheimer''s disease. Nat Genet 7 (1) : 74-78, (1994).

Velazquez-Perez, L., Rodriguez-Labrada, R., et al. A comprehensive review of spinocerebellar ataxia type 2 in Cuba. Cerebellum 10 (2) : 184-198, (2011).

Velazquez-Perez, L., Seifried, C., et al. Saccade velocity is controlled by polyglutamine size in spinocerebellar ataxia 2. Ann Neurol 56 (3) : 444-447, (2004).

Verkerk, A. J., Pieretti, M., et al. Identification of a gene (FMR-1) containing a CGG repeat coincident with a breakpoint cluster region exhibiting length variation in fragile X syndrome. Cell 65 (5) : 905-914, (1991).

Villace, P., M. Marion, R., et al. The composition of Staufen-containing RNA granules from human cells indicates their role in the regulated transport and translation of messenger RNAs. Nucleic Acids Res 32 (8) : 2411-2420, (2004).

Wada, Y., Sun-Wada, G. H., et al. Microautophagy in the visceral endoderm is essential for mouse early development. Autophagy 9 (2) : 252-254, (2013).

Wadia, N. H. and Swami, R. K. A new form of heredo-familial spinocerebellar degeneration with slow eye movements (nine families). Brain 94 (2) : 359-374, (1971).

Wen, F. C., Li, Y. H., et al. Down-regulation of heat shock protein 27 in neuronal cells and non-neuronal cells expressing mutant ataxin-3. FEBS Lett 546 (2-3) : 307-314, (2003).

Wullner, U. Genes implicated in the pathogenesis of spinocerebellar ataxias. Drugs Today (Barc) 39 (12) : 927-937, (2003).

Wyttenbach, A., Sauvageot, O., et al. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin. Hum Mol Genet 11 (9) : 1137-1151, (2002).

Wyttenbach, A., Swartz, J., et al. Polyglutamine expansions cause decreased CRE-mediated transcription and early gene expression changes prior to cell death in an inducible cell model of Huntington''s disease. Hum Mol Genet 10 (17) : 1829-1845, (2001).

Yabe, I., Sasaki, H., et al. Spinocerebellar ataxia type 14 caused by a mutation in protein kinase C gamma. Arch Neurol 60 (12) : 1749-1751, (2003).

Yu, Y. C., Kuo, C. L., et al. Decreased antioxidant enzyme activity and increased mitochondrial DNA damage in cellular models of Machado-Joseph disease. J Neurosci Res 87 (8) : 1884-1891, (2009).

Zhang, S. P., Niu, Y. N., et al. Role of autophagy in acute myeloid leukemia therapy. Chin J Cancer 32 (3) : 130-135, (2013).

Zhu, J. H., Horbinski, C., et al. Regulation of autophagy by extracellular signal-regulated protein kinases during 1-methyl -4-phenylpyridin ium–induced cell death. Am J Pathol 170 (1) : 75-86, (2007).

Zhuchenko, O., Bailey, J., et al. Autosomal dominant cerebellar ataxia (SCA6) associated with small polyglutamine expansions in the alpha 1A-voltage-dependent calcium channel. Nat Genet 15 (1) : 62-69, (1997).

Zortea, M., Armani, M., et al. Prevalence of inherited ataxias in the province of Padua, Italy. Neuroepidemiology 23 (6) : 275-280, (2004).

Zu, L., Figueroa, K. P., et al. Mapping of a new autosomal dominant spinocerebellar ataxia to chromosome 22. Am J Hum Genet 64 (2) : 594-599, (1999).


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1. 李選(1990)。音樂治療與應用。長庚護理,1(1),62-71。
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3. 吳國瑄(2005)。節奏律動引導幼兒肢體潛能開發。幼兒保育學刊,1,188-203。
4. 黃鳳怡(2001)。從生理特徵談唐氏症兒童的身體活動。學校體育雙月刊,11(2),88-92。
5. 黃榮真(2006c)。協同行動取向之國小特教班學生音樂教學活動方案實踐與反思。花蓮教育大學學報,23,209-236。
6. 黃靄雯、廖華芳(2004)。腦性麻痺兒以任務為導向之步行訓練成效---兩個個案報告。 物理治療 2004;29(4):254-262
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