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研究生:何堃志
研究生(外文):KUN-CHIH HO
論文名稱:以靜電紡絲方法製備人工皮膚的生物相容性研究
論文名稱(外文):The Study in Biocompatibility of Gelatin Base Artificial Skin Fabricated with Electrospinning
指導教授:徐中華
指導教授(外文):Cheung-Hwa Hsu
口試委員:張朝城、鄭耀昌
口試日期:2013-07-01
學位類別:碩士
校院名稱:國立高雄應用科技大學
系所名稱:模具系碩士在職專班
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:53
中文關鍵詞:靜電紡絲人造的聚乳酸甘醇酸
外文關鍵詞:electrospinningartificialPLGA
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  • 下載下載:66
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為解決植皮手術供皮來源之問題,因而有以組織工程方式製作出人工皮膚替代物之開發與上市,現有市售人工皮膚替代物主要是利用膠原蛋白作為細胞基質,結合成纖維母細胞取代真皮層,再加上角質細胞取代表皮層。然而現有人工皮膚替代物面臨幾個問題,包括(1)基質強度不足導致移植物容易脆化被降解,(2)微血管化延遲造成局部缺血而導致移植物活性受損,並造成移植效率低落。本研究希望透過靜電紡絲的方法,利用明膠(gelatin)與聚乳酸甘醇酸(PLGA)高分子組構適合皮膚細胞生長的人工皮膚替代物,以克服市售人工皮膚替代物面臨之問題。由靜電紡絲技術製造3D結構的網狀支架,並且進行體外細胞毒性測試以及生物基質機械強度測試架構出次微米立體結構化的人工皮膚替代物。
In order to resolve aforementioned problems encountered during grafting, cultured skin substitute has been developed. The existing cultured skin substitutes found in the market use gelatin as a matrix for cell attachment and growth. In principle, gelatin matrix is inoculated with fibroblast as dermis substitute and inoculated with keratinocyte as epidermis substitute. However the existing cultured skin substitutes have two drawbacks including (1) lack of mechanical strength resulted in easy degradation and shrinkage; (2) delayed vascularization due to ischemia that reduces graft bioactivity, leading to poor grafting efficacy.The present thesis introduced a cultured skin substitute with gelatin based biomaterials fabricated with electrospinning which can overcome the questions associated with current culture skin substitutes. With the advancement of electrospinning technology, gelatin and PLGA matrix formation technique, skin cell separation and the mass cultured technology and endothelial cell isolation technique have successfully constructed.
摘 要 I
英文摘要 II
誌 謝 III
目錄 IV
表目錄 VI
圖目錄 VII
第一章 研究背景 1
1.1 前言 1
1.2皮膚的構造及功能 3
1.3 研究目的 7
第二章 文獻回顧 8
2.1 傷口敷料的發展演進 12
2.2 人工皮膚的相關研究 15
2.3 人工皮膚市售商品 17
2.4 靜電紡絲法 21
2.5 生物材料機械性質檢測方法 24
2.6 人工皮膚上市流程 26
第三章 材料與方法 28
3.1明膠材料 28
3.2聚乳酸/甘醇酸材料 29
3.3 市售品(TEGADERM FILM, TDF) 30
3.4細胞支架材料設計原則及人工皮膚所需之條件 31
3.5檢測方法建立 32
3.5.1觀察支架型態 32
3.5.2細胞活性測試 32
3.5.3機械性質測試 34
3.5研究架構 35
第四章 結果與討論 36
4.1 GELATIN/PLGA及巿售人工皮膚表面形態觀察 36
4.2 生物相容性測試 40
4.3機械性質測試 42
第五章 結論與未來展望 49
5.1結論 49
5.2未來展望 51
參考文獻 52

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