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研究生:黃欣珮
研究生(外文):Hsin-Pei Huang
論文名稱:利用大豆豆渣液態培養Aureobasidium pullulans NCH-218生產聚甘露糖酶及酵素特性分析
論文名稱(外文):Production of Mannanase from Aureobasidium pullulans NCH-218 in Submerged Cultivation on Soybean Okara and Characterization of Mannanase
指導教授:陳錦樹陳錦樹引用關係
口試委員:黃至盛林澤群
口試日期:2013-07-31
學位類別:碩士
校院名稱:國立中興大學
系所名稱:食品暨應用生物科技學系所
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:98
中文關鍵詞:半纖維素酶Aureobasidium pullulans聚甘露糖酶大豆豆渣酵素純化甘露寡糖
外文關鍵詞:soybean okaraAureobasidium pullulanshemicellulasemannanaseenzyme purificationmanno-oligosaccharides
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本研究使用一株具有生產半纖維素酶能力之分離株,Aureobasidium pullulans NCH-218,以大豆豆渣為主要基質進行聚甘露糖酶生產、培養條件、純化及酵素特性分析。首先以搖瓶培養方式(載液量 50 mL,於 250 mL 之平底三角瓶)探討 A. pullulans NCH-218 生產聚甘露糖酶較適液態培養基組成與培養條件。實驗結果顯示,以大豆豆渣濃度 3%(w/v)為基礎,添加 1%(w/v)刺槐豆膠作為生產酵素誘導物,0.4 % (NH4)2SO4 為額外氮源,培養溫度30℃,起始 pH 5.4-5.5,菌液接種量 1%(v/v)(1.0 × 105 cell/mL),振盪速率 130 rpm,收集培養三天後之酵素液,其聚甘露糖酶活性為 3.55 U/mL。A. pullulans NCH-218 聚甘露糖酶經超過濾濃縮、硫酸銨(60-80 %)及DEAE-Sepharose Fast Flow 離子交換層析等步驟純化後,進行純化聚甘露糖酶性質分析。由 SDS-PAGE 結果推論此酵素分子量約 34 kDa,最適反應 pH 為 3.0,在 pH 3.0-6.0 間活性仍高且穩定性良好,最適反應溫度為 60℃,溫度低於 40℃ 熱穩定性較佳。對聚甘露糖類具專一性,以刺槐豆膠作為酵素基質之水解產物為甘露寡糖,以甘露二糖及甘露三糖為主。

A hemicellulase-producing fungal isolate, Aureobasidium pullulans NCH-218, isolated from our lab(Huang, 2012) was used in the study. The purpose of the study was to produce, purify and characterize the mannanase from A. pullulans NCH-218. The optimal medium and cultivation conditions for A. pullulans NCH-218 was determined in shaking flask(capacity 50mL/250 mL). The results showed that 3% soybean okara was used as basal medium with 1% locust bean gum as additional carbon source, 0.4% (NH4)2SO4 as additional nitrogen source. Incubation temperature at 30℃, initial pH at 5.4-5.5, inoculum size 1%(v/v)(1.0 × 105 cell/mL), and shaking rate at 130 rpm gave the best result for the enzyme production. The maximum activity approximately 3.55 U/mL in the culture broth was obtained after 3 days under the above conditions. The broth filtrate from A. pullulans NCH-218 was subsequently purified by ultrafiltration, ammonium sulfate precipitation(60-80%) and DEAE-Sepharose Fast Flow ion exchange chromatography. The nearly purified mannanase had molecular weight around 34 kDa. The optimum pH was pH 3.0, while the enzyme remained quite stable in pH ranging pH from 3.0 to 6.0. The optimum temperature was 60℃, and the enzyme was stable at temperature under 40℃. The purified mannanase demonstrate a specificity towards mannans-based substrates. The hydrolysis products from locust
bean gum was manno-oligosaccharides, mainly mannobiose and mannotriose.


摘要 ii
Abstract iii
目次 iv
圖目次 vii
表目次 ix
第一章 前言 1
第二章 文獻回顧 2
一、 大豆 2
(一) 大豆之簡介 2
(二) 大豆之機能性 2
二、 大豆豆渣 5
(一) 大豆豆渣之組成 5
(二) 大豆豆渣之應用與相關研究 5
三、 半纖維素之簡介 8
四、 聚甘露糖之介紹 9
(一) 聚甘露糖簡介 9
(二) 聚甘露糖結構與種類 9
五、 聚甘露糖酶之介紹 14
(一) 聚甘露糖酶來源 14
(二) 聚甘露糖酶種類 14
(三) 聚甘露糖酶之應用 18
六、 Aureobasidium pullulans之介紹 21
(一) Aureobasidium pullulans 之生長與分布 21
(二) Aureobasidium pullulans 之應用 22
第三章 材料與方法 26
一、 實驗材料 26
(一) 實驗原料 26
(二) 實驗菌株 26
(三) 培養基 26
(四) 化學藥劑 27
二、 儀器設備 28
三、 實驗架構 30
第四章 結果與討論 39
一、 大豆豆渣基本成分分析 39
二、 A. pullulans NCH-218 之生長曲線 39
三、 生產聚甘露糖酶較適培養條件之探討 42
(一) 豆渣濃度 42
(二) 碳源種類 44
(三) 碳源濃度 45
(四) 氮源種類 48
(五) 起始 pH 50
(六) 培養溫度 52
(七) 菌液接種量 53
(八) 振盪速率 56
(九) 較適培養條件下時間對聚甘露糖酶活性影響 56
四、 聚甘露糖酶粗酵素液性質分析 59
(一) 粗酵素液製備 59
(二) 最適反應 pH 59
(三) pH 穩定性 59
(四) 最適反應溫度 62
(五) 熱穩定性 62
五、 酵素純化 66
(一) 超過濾濃縮 66
(二) 硫酸銨沉澱劃分 66
(三) DEAE-Sepharose FF 離子交換層析 66
六、 純化後聚甘露糖酶性質分析 69
(一) 酵素純度鑑定 69
(二) 原態電泳(native-PAGE) 69
(三) 最適反應 pH 73
(四) pH 穩定性 73
(五) 最適反應溫度 76
(六) 熱穩定性 76
(七) 基質特異性 76
(八) 水解產物分析 79
第五章 結論 82
第六章 未來展望 83
第七章 參考文獻 84


皮雄娥、費笛波、王龍英、袁超。2005。大豆黄酮及其生理功能的研究進展。飼料工業。26(4):11-14.
吳昭慧、連大進。1998。毛豆之營養與效用。台南區農業專訊 26:19-21。
梁文薔。2002。健身防疾保平安發揚國粹吃大豆。健康世界 200:89-95。
陳世爵、陳潤卿。1981。黄豆油與黄豆食品手册。台灣。
陳暐楨。2006。豆渣堆肥過程中化學及微生物相變化,國立高雄第一科技大學環境與安全衛生工程系。碩士論文。
黃詩淳。2012。半纖維素酶生產菌株之篩選、培養條件與 Aureobasidium pullulans NCH-218 聚木糖酶酵素特性探討,中興大學食品暨應用生物科技學系。碩士論文。
曾富生、吳詩都。1996。農藝。東大圖書出版社。台北市。
Ademark, P., Varga, A., Medve, J., Harjunpaa, V., Drakenberg, T., Tjerneld, F. and Stalbrand, H. 1998. Softwood hemicellulose-degrading enzymes from Aspergillus nige: Purification and properties of a β-mannanase. Journal of Biotechnology. 63(3):199-210.
Alemdar, A., and Sain, M. 2008. Isolation and characterization of nanofibers from agricultural residues–Wheat straw and soy hulls. Bioresource Technology. 99(6):1664-1671.
Araujo, A., and Ward, O. P. 1990. Extracellular mannanases and galactanases from selected fungi. Journal of industrial Microbiology. 6(3):171-178.
Arditi, T., and Meredith, T. 1999. Soy isoflavones and saponins: A natural soy solution to age-old problem. Agro Food Industry Hi Tech. 12-14.
Arisan-Atac, I., Hodits, R., Kristufek, D., and Kubicek, C. P. 1993. Purification, and characterization of a β-mannanase of Trichoderma reesei C-30. Applied Microbiology and Biotechnology. 39(1):58-62.
Aspinall, G. O. 1959. Structural chemistry of the hemicelluloses. Advances in Carbohydrate Chemistry. 14:429-468.
Aspinall, G. O., Hunt, K., and Morrison, I. M. 1966. Polysaccharides of soy-beans. Part II. Fractionation of hull cell-wall polysaccharides and the structure of a xylan. Journal of Chemistry Society C. 1945-1949.
Babjeva, I., and Reshetova, I. 1998. Yeast resources in natural habitats at polar circle latitude. Food Technology and Biotechnology. 36(1):1-5.
Biely, P. 1985. Microbial xylanolytic systems. Trends in Biotechnology. 3(11):286-290.
Blibech, M., Ghorbel, R. E., Fakhfakh, I., Ntarima, P., Piens, K., Bacha, A. B., and Chaabouni, S. E. 2010. Purification and characterization of a low molecular weight of β-mannanase from Penicillium occitanis Pol6. Applied Biochemistry and Biotechnology. 160(4):1227-1240.
Bourne, M. C., Clemente, M. G., and Banzon, J. 1976. Survey of the suitability of thirty cultivars of soybeans for soymilk manufacture. Journal of Food Science. 41(5):1204-1208.
Bradbury, A. G., and Halliday, D. J. 1990. Chemical structures of green coffee bean polysaccharides. Journal of Agricultural and Food Chemistry. 38(2):389-392.
Bresolin, T. M., Sandler, P. C., Reicher, F., Sierakowski, M. R., Rinaudo, M., and Ganter, J. L. M. 1997. Viscometric studies on xanthan and galactomannan systems. Carbohydrate Polymers. 33(2-3):131-138.
Buckeridge, M. S., Pessoa dos Santos, H., and Tine, M. A. S. 2000. Mobilisation of storage cell wall polysaccharides in seeds. Plant Physiology and Biochemistry. 38(1), 141-156.
Charrier, M., and Rouland, C. 2001. Mannan-degrading enzymes purified from the crop of the brown garden snail Helix aspersa Muller
(Gastropoda Pulmonata). Journal of Experimental Zoology. 290(2):125-135.
Chen, B. K., and Diosady, L. L. 2003. Enzymatic aqueous processing of coconuts. International Journal of Applied Science, Engineering and Technology. 1:55-61.
Chi, Z., Wang, F., Chi, Z., Yue, L. Liu, G., and Zhang, T. 2009. Bioproducts from Aureobasidium pullulans, a biotechnologically important yeast. Applied Microbiology and Biotechnology. 82(5):793–804.
Clifford, M. N. 1985. Chemical and physical aspects of green coffee and coffee products. Coffee. 305-374.
Comfort, D. A., Chhabra, S. R., Conners, S. B., Chou, C. J., Epting, K. L., Johnson, M. R., Jones, K. L., Sehgal, A. C., and Kelly, R. M. 2004. Strategic biocatalysis with hyperthermophilic enzymes. Green Chemistry. 6(9):459-465.
Cui, F., Shi, J., and Lu, Z. 1999. Production of neutral beta-mannanase by Bacillus subtilis and its properties. Wei sheng wu xue bao= Acta microbiologica Sinica. 39(1):60.
Considine, P. J., and Coughlan, M. P. 1989. Production of carbohydrate-hydrolysing enzyme blends by solid-state fermentation. Enzyme System for Lignocellulose Degradation. Elsevier Applied Science, London. 273-281.
Coughlan, M. P., Tuohy, M. G., Filho, E. X. F., Puls, J., Claeyssens, M., Vrsanska, M., and Hughes, M. M. 1993. Enzymological aspects of microbial hemicellulases with emphasis on fungal systems. Hemicellulose and Hemicellulases. Portland Press, London:53-84.
Cuevas, W. A., Kantelinen, A., Tanner, P., Bodie, B., and Leskinen, S. 1996. Purification and characterization of novel mannanases used in pulp bleaching. Biotechnology in the Pulp and Paper Industry. Facultas-Universitatsverlag. Vienna. 123-126.
De Hoog, G. S. 1993. Evolution of black yeasts: possible adaptation to the human host. Antonie van Leeuwenhoek. 63(2):105-109.
Dea, I. C. M. and Morrison, A. 1975. Chemistry and interactions of seed galactomannans. Advances in Carbohydrate Chemistry and Biochemistry. 31:241-312.
Dekker, R. F. 1985. Biodegradation of the hemicelluloses. Biosynthesis and Biodegradation of Wood Components. 505-532.
Dekker, R. F., and Richards, G. N. 1976. Hemicellulases: their occurrence, purification, properties and mode of action. Advances in Carbohydrate Chemistry and Biochemistry. 32: 277-352.
Deshpande, M. S., Rale, V. B., and Lynch, J. M. 1992. Aureobasidium pullulans in applied microbiology: a status report. Enzyme and Microbial Technology. 14(7):514–527.
Dey, P. M. 1978. Biochemistry of plant galactomannans. Advances in Carbohydrate Chemistry and Biochemistry. 35:341-376.
Dhawan, S., and Kaur, J. 2007. Microbial mannanases: an overview of production and applications. Critical Reviews in Biotechnology. 27(4):197-216.
Duffaud, G. D., McCutchen, C. M., Leduc, P., Parker, K. N., and Kelly, R. M. 1997. Purification and characterization of extremely thermostable beta-mannanase, beta-mannosidase, and alpha-galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana 5068. Applied and Environmental Microbiology. 63(1):169-177.
El-Helow, E. R., and Khattab, A. A. 1996. The development of a Bacillus subtilis 168 culture condition for enhanced and accelerated beta-mannanase production. Acta Microbiologica et Immunologica Hungarica. 43(4):289.
El-Helow, E. R., Sabry, S. A., and Khattab, A. A. 1997. Production of α-mannanase by B. subtilis from agro-industrial by-products: screening and optimization. Antonie van Leeuwenhoek. 71(3):189-193.
Eneyskaya, E. V., Sundqvist, G., Golubev, A. M., Ibatullin, F. M., Ivanen, D. R., Shabalin, K. A., and Kulminskaya, A. A. 2009. Transglycosylating and hydrolytic activities of the β-mannosidase from Trichoderma reesei. Biochimie. 91(5):632-638.
Eriksson, K. E. L., Blanchette, R. A., and Ander, P. 1990. Biodegradation of hemicelluloses. In Microbial and Enzymatic Degradation of Wood and Wood Components (pp. 181-224). Springer Berlin Heidelberg.
Fernandes, P. B. 1995. Influence of galactomannan on the structure and thermal behaviour of xanthan/galactomannan mixtures. Journal of Food Engineering. 24(2):269-283.
Forage, R. G., Harrison, D. E. F., and Pitt, D. E. 1985. Effect of environment on microbial activity.
Friedman, M., and Brandon, D. L. 2001. Nutritional and health benefits of soy proteins. Journal of Agricultural and Food Chemistry. 49(3):1069-1086.
Galbe, M., and Zacchi, G. 2002. A review of the production of ethanol from softwood. Applied Microbiology and Biotechnology. 59(6):618-628.
Gerber, P. J., Heitmann, J. A., Joyce, T. W., Buchert, J., and Siika-Aho, M. 1999. Adsorption of hemicellulases onto bleached kraft fibers. Journal of Biotechnology. 67(1):67-75.
Gibbs, P. A., and Seviour, R. J. 1996. Does the agitation rate and/or oxygen saturation influence exopolysaccharide production by Aureobasidium pullulans in batch culture?. Applied Microbiology and Biotechnology. 46(5-6):503-510.
Gibbs, P. A., Seviour, R. J., and Schmid, F. 2000. Growth of filamentous fungi in submerged culture: problems and possible solutions. Critical Reviews in Biotechnology. 20(1):17-48.
Gomes, J., Terler, K., Kratzer, R., Kainz, E., and Steiner, W. 2007. Production of thermostable β-mannosidase by a strain of Thermoascus aurantiacus: Isolation, partial purification and characterization of the enzyme. Enzyme and Microbial Technology. 40(4):969-975.
Goyal, M., Kalra, K. L., Sareen, V. K., and Soni, G. 2008. Xylanase production with xylan rich lignocellulosic wastes by a local soil isolate of Trichoderma viride. Brazilian Journal of Microbiology. 39(3):535-541.
Grant Reid, J. S., Davies, C., and Meier, H. 1977. Endo-β-mannanase, the leguminous aleurone layer and the storage galactomannan in germinating seeds of Trigonella foenum-graecum L. Planta. 133(3):219-222.
Groot, S. P., Kieliszewska-Rokicka, B., Vermeer, E., and Karssen, C. M. 1988. Gibberellin-induced hydrolysis of endosperm cell walls in gibberellin-deficient tomato seeds prior to radicle protrusion. Planta. 174(4):500-504.
Gubitz, G. M., Hayn, M., Urbanz, G., and Steiner, W. 1996. Purification and properties of an acidic α-mannanase from Sclerotium rolfsii. Journal of Biotechnology. 45(2):165-172.
Guermani, L., Villaume, C., Bau, H. W., Chandrasiri, V., Nicolas, J. P., and Mejean, L. 1992. Composition and nutritional value of okara fermented by Rhizopus oligosporus. Sciences des Aliments. 12(3):441-451.
Gunde-Cimerman, N., Zalar, P., de Hoog, S., and Plemenitaš, A. 2000. Hypersaline waters in salterns – natural ecological niches for halophilic black yeasts. FEMS Microbiology Ecology. 32(3):235-240.
Gupta, R., Gigras, P., Mohapatra, H., Goswami, V. K., and Chauhan B. 2003. Microbial α-amylases: a biotechnological perspective. Process Biochemistry. 38(11):1599–616.
Halmer, P., and Bewley, J. D. 1979. Mannanase production by the lettuce endosperm: control by the embryo. Planta. 144(4):333-340.
Hatada, Y., Takeda, N., Hirasawa, K., Ohta, Y., Usami, R., Yoshida, Y., and Horikoshi, K. 2005. Sequence of the gene for a high-alkaline mannanase from an alkaliphilic Bacillus sp. strain JAMB-750, its expression in Bacillus subtilis and characterization of the recombinant enzyme. Extremophiles. 9(6):497-500.
Harjanti, D. W., Sugawara, Y., Al-Mamun, M. and Sano, H. 2012. Effects of replacing concentrate with soybean curd residue silage on ruminal characteristics, plasma leucine and glucose turnover rates in sheep. Journal of Animal Science Advances. 2(4):361-374.
Hasegawa, Y. 1998. Manufacture of high-strength ceramics by utilizing pozzolanic reaction and recycling of fly ashes and okara. Chemistry Abstract. 128, 131458.
He, X., Liu, N., Li, W., Zhang, Z., Zhang, B., and Ma, Y. 2008. Inducible and constitutive expression of a novel thermostable alkaline β-mannanase from alkaliphilic Bacillu sp. N16-5 in Pichia pastoris and characterization of the recombinant enzyme. Enzyme and Microbial Technology. 43(1):13-18.
Hiji, Y. 1986. Method for inhibiting increase in blood sugar content. US Patent Office, Pat. No. 4 629 725.
Hsiao, H. Y., Anderson, D. M., and Dale, N. M. 2006. Levels of β-mannan in soybean meal. Poultry Science. 85(8):1430-1432.
Hongpattarakere, T. 2002. Hyperthermostable cellulolytic and hemicellulolytic enzymes and their biotechnological applications. Songklanakarin Journal of Science and Technology. 24(3):481-491.
Howard, R. L., Abotsi, E., Van Rensburg, E. J., and, S. 2004. Lignocellulose biotechnology: issues of bioconversion and enzyme production. African Journal of Biotechnology. 2(12):602-619.
Ishurd, O., Kermagi, A., Elghazoun, M., and Kennedy J. F. 2006. Structural of a glucomannan from Lupinus varius seed. Carbohydrate Polymers. 65(4):410-413.
Jackson, M. E., Geronian, K., Knox, A., McNab, J., and McCartney, E. 2004. A dose-response study with the feed enzyme beta-mannanase in broilers provided with corn-soybean meal based diets in the absence of antibiotic growth promoters. Poultry Science. 83(12):1992-1996.
Jacobsen, J. V., and Pressman, E. 1979. A structural study of germination in celery (Apium graveolens L.) seed with emphasis on endosperm breakdown. Planta 144(3):241-248.
Jeffries, T. W. and Kurtzman C. P.. 1994. Strain selection, taxonomy, and genetics of xylose-fermenting yeasts. Enzyme and Microbial Technology. 16(11):922 –932.
Jiang, Z., Wei, Y., Li, D., Li, L., Chai, P., and Kusakabe, I. 2006. High-level production, purification and characterization of a thermostable β-mannanase from the newly isolated Bacillus subtilis WY34. Carbohydrate Polymers. 66(1): 88-96.
Kansoh, A. L., and Nagieb, Z. A. 2004. Xylanase and mannanase enzymes from Streptomyces galbus NR and their use in biobleaching of softwood kraft pulp. Antonie Van Leeuwenhoek. 85(2):103-114.
Kataoka, N., and Tokiwa, Y. 1998. Isolation and characterization of an active mannanase-producing anaerobic bacterium, Clostridium tertium KT-5A, from lotus soil. Journal of Applied Microbiology. 84(3):357-367.
Khare, S. K,. Jha, K., and Gandhi, A. P. 1993. Physicochemical and functional properties of okara protein isolate. Journal of Dairying Foods and Home Science. 12(3-4):132-136.
Khare, S. K., Jha, K.; and Sinha, L. K. 1995. Preparation and nutritional evaluation of okara fortified biscuits. Journal of Dairying, Foods and Home Sciences. 14(1-2): 91-94.
Kim, D. Y., Ham, S. J., Lee, H. J., Kim, Y. J., Shin, D. H., Rhee, Y. H., and Park, H. Y. 2011. A highly active endo-β-1, 4-mannanase produced by Cellulosimicrobiumsp. strain HY-13, a hemicellulolytic bacterium in the gut of Eisenia fetida. Enzyme and Microbial Technology. 48(4):365-370.
Kobayashi, T. Fat- and oil-coated okara for cakes and its manufacture. 1997. Chemistry Abstract. 127:358274.
Kobayashi, Y., Echigen, R., Mada, M., and Mutai, M. 1987. Effect of hydrolyzate of konjac mannan and soyabean oligisaccharides on intestinal flora in man and rats. Intestinal Flora and Food Factors. Gakkai Shuppan Centre, Tokyo. 79–97.
Kojima, M., Tachikake, N., Kyotani, Y., Konno, K., Maruo, S., Yamamoto, M., and Ezure, Y. 1995. Effect of dissolved oxygen and pH on moranoline (1-deoxynojirimycin) fermentation by Streptomyces lavendulae. Journal of Fermentation and Bioengineering. 79(4):391-394.
Kote, N. V., Patil, A. G. G., and Mulimani, V. H. 2009. Optimization of the production of thermostable endo-β-1, 4 mannanases from a newly isolated Aspergillus niger gr and Aspergillus flavus gr. Applied Biochemistry and Biotechnology. 152(2):213-223.
Kremnicky, L., and Biely, P. 1997. β-Mannanolytic system of Aureobasidium pullulans. Archives of Microbiology. 167(6):350-355.
Kremnicky, L., Slavikova, E., Mislovičova, D., and Biely, P. 1996. Production of extracellular β-mannanases by yeasts and yeast-like microorganisms. Folia Microbiologica. 41(1):43-47.
Kronenberg, H. J., and Hang, Y. D. 1984. Biochemical changes in okara during meitauza fermentation. Nutrition Reports International. 30:439-443.
Kudanga, T., and Mwenje, E. 2005. Extracellular cellulase production by tropical isolates of Aureobasidium pullulans. Canadian Journal of Microbiology. 51(9):773–776.
Kumar, C. G., and Takagi, H. 1999. Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnology Advances. 17(7):561-594.
Laere, K. M., Hartemink, J. V., Beldman, R., Pitson, G., Dijkema, S., and Schols, C. 1999. Transglycosidase activity of Bifidiobacterium adolescentis DSM 20083 galactosidase. Applied Microbiology and Biotechnology. 52(5):681-688.
Lattaud, C., Locati, S., Mora, P., Rouland, C., and Lavelle, P. 1998. The diversity of digestive systems in tropical geophagous earthworms. Applied Soil Ecology. 9(1-3):189-195.
Lee, R. L., Charles, E. W., and Tillman, U. G. 1999. Biocommodity engineering. Biotechnology Progress. 15:777-793.
Li, C. H., Song, L. S., Zhao, J. M., Zhu, L., Zou, H. B., Zhang, H., Wang, Z. H., and Cai, Z. H. 2007. Preliminary study on a potential antibacterial peptide derived from histone H2A in hemocytes of scallop Chlamys farreri. Fish and Shellfish Immunology. 22(6):663–672.
Liepman, A. H., Nairn, C. J., Willats, W. G. T., Sorensen, I., Roberts A. W., and Keegstra, K. 2007. Functional genomic analysis supports conservation of function among cellulose synthase-like a gene family members and suggests diverse roles of mannans in plants. Plant Physiology. 143(4):1991-1893.
Lin, T. C. 2003. Production, purification and characterization of mannanase from Aspergillus niger NCH-189 and its application on the preparation of mannooligosaccharides.
Lin, T. C., & Chen, C. 2004. Enhanced mannanase production by submerged culture of Aspergillus niger NCH-189 using defatted copra based media. Process Biochemistry. 39(9):1103-1109.
Liu, K. 1999. Soybeans: chemistry, technology, and utilization. Chapman and Hall. 25-411.
Liu, Z. Q., Li, X. Y., Chi, Z. M., Wang, L., Li, J., and Wang, X. H. 2008. Cloning, characterization and expression of the extracellular lipase gene from Aureobasidium pullulans HN2-3 isolated from sea saltern. Antonie van Leeuwenhoek. 94(2):245–255.
Ma, C. L., Ni, X. M., Chi, Z. M., Ma, L. Y., and Gao, L. M. 2007. Purification and characterization of an alkaline protease from the marine yeast Aureobasidium pullulans for bioactive peptide production from different sources. Marine Biotechnology. 9(3):343–351.
Ma, C. Y., Liu, W. S., Kwok, K. C., and Kwok, F. 1996. Isolation and characterization of proteins from soymilk residue (okara). Food Research International. 29(8):799-805.
Ma, Y., Xue, Y., Dou, Y., Xu, Z., Tao, W., and Zhou, P. 2004. Characterization and gene cloning of a novel β-mannanase from alkaliphilic Bacillus sp. N16-5. Extremophiles. 8(6):447-454.
Mabrouk, M. E., and El Ahwany, A. M. 2008. Production of 946-mannanase by Bacillus amylolequifaciens 10A1 cultured on potato peels. African Journal of Biotechnology. 7(8).
MaCleary, B.V., and Matheson, N. K. 1975. Galactomannan structure and β-mannanase and β-mannosidase activity in germinating legume seeds. Phytochemistry. 14 (5):1187-1194.
Maijala, P., Raudaskoski, M., and Viikari, L. 1995. Hemicellulolytic enzymes in P-and S-strains of Heterobasidion annosum. Microbiology. 141(3):743-750.
Matsuo, M. 1989a. Morphological and physicochemical properties and composition of “okara” fermented with Rhizopus oligosporus. Japan Society of Nutrition and Food Science. 42(2):173-178.
Matsuo, M. 1989b. Morphological, compositional and physicochemical properties of “okara” fermented by Aspergillus oryzae. Journal of the Agricultural Chemical Society of Japan. 63(11):1765-1770.
Matsuo, M. 1990. Effects of rice bran on properties of “okara tempe”. Nippon Nogeikagaku Kaishi. 64 (7):1237-1240.
McCutchen, C. M., Duffaud, G. D., Leduc, P., Petersen, A. R., Tayal, A., Khan, S. A., and Kelly, R. M. 1996. Characterization of Extremely Thermostable Enzymatic Breakers (α-1, 6-Galactosidase and β-1, 4= Mannanase) from the hyperthermophilic bacterium Thermotoga neapolitana 5068 for hydrolysis of guar gum. Biotechnology and Bioengineering. 52:332-339.
Meier, H., and Reid, J. S. G. 1982. Reserve Polysaccharides other than starch in higher plants. In Plant Carbohydrates I. (pp. 418-471). Springer Berlin Heidelberg.
Michael, J. C., and Watkinson, S. C. 1997. Fungal cell and vegetative growth. In The Fungi, Acdemic Press: U. S. A.
Moreira, L. R. S. 2008. An overview of mannan structure and mannan-degrading enzyme systems. Applied Microbiology and Biotechnology. 79(2):165-178.
Mou, H., Zhou, F., Jiang, X., & Liu, Z. 2011. Production, purification and properties of β-mannanase from soil bacterium Bacillus circulans M-21.. Journal of Food Biochemistry. 35(5):1451-1460.
Mounir, R., Durieux, A., Bodo, C., Allard, C., Simon, J. P., Achbani, E. H., El-Jaafari, S., Douira, A., and Jijakli, M. H. 2007. Production, formulation and antagonistic activity of the biocontrol like-yeast Aureobasidium pullulans against Penicillium expansum. Biotechnology Letters. 29(4):553-559.
Mudau, M. M., and Setati, M. E. 2008. Partial purification and characterization of endo-β-1, 4-mannanases from Scopulariopsis candida strains isolated from solar salterns. African Journal of Biotechnology. 7(13).
Nakajima, N., and Matsuura, Y. 1997. Purification and characterization of konjac glucomannan degrading enzyme from anaerobic human intestinal bacterium, Clostridium butyricum-Clostridium beijerinckii group. Bioscience, Biotechnology, and Biochemistry. 61(10):1739-1742.
Naoi, S., Hatakeyama, T., and Hatakeyama, H. 2002. Phase transition of locust bean gum-, tara gum-and guar gum-water systems. Journal of Thermal Analysis and Calorimetry. 70(3):841-852.
Nicolas, P., Raetz, E., Reymond, S., and Sauvegeat, J. L. 1998. Hydrolysis of the galactomannans of coffee extract with immobilized β-mannanase. Patent US 5714183.
Noguchi, A. 1987. Method for the preparation of textured soybean draff. U.S. Patent. NO. 4 ,642,241.
Nolan, A. L. 1983. Tradition combines with technology: Flavored tofu. Food Engineering. 55(7):45.
Norita, S., Rosfarizan, M., and Ariff, A. B. 2010. Evaluation of the activities of concentrated crude mannan-degrading enzymes produced by Aspergillus niger. Malaysian Journal of Microbiology. 6(2):171-180.
Northcote, D. H. 1972. Chemistry of the plant cell wall. Annual Review of Plant Physiology. 23(1):113–132.
Nunes, F. M., Reis, A., Domingues, M. R., and Coimbra, M. A. 2006. Characterization of galactomannan derivatives in roasted coffee beverages. Journal of Agricultural and Food Chemistry. 54(9):3428–3439.
Odetallah, N. H., Ferket, P. R., Grimes, J. L., and McNaughton, J. L. 2002. Effect of mannan-endo-1, 4-beta-mannosidase on the growth performance of turkeys fed diets containing 44 and 48% crude protein soybean meal. Poultry Science. 81(9):1322-1331.
Ohno, A., Ano, T. and Shoda, M. 1993. Production of the antifungal peptide antibiotic, iturin by Bacillus subtilis NB22 in solid- state fermentation. Journal of Fermentation and Bioengineering. 75(1):23-27.
Olsson, L., Christensen, T. M., Hansen, K. P., and Palmqvist, E. A. 2003. Influence of the carbon source on production of cellulases, hemicellulases and pectinases by Trichoderma reesei Rut C-30. Enzyme and Microbial Technology. 33(5):612-619.
Ootsuka, S., Saga, N., Suzuki, K. I., Inoue, A., and Ojima, T. 2006. Isolation and cloning of an endo-β-1, 4-mannanase from Pacific abalone Haliotis discus hannai. Journal of Biotechnology. 125(2):269-280.
O’Toole, D. K. 1999. Characteristics and use of okara, the soybean residue from soy milk production – a review. Journal of Agricultural Food Chemistry. 47(2):363-371.
Perret, S., Belaich, A., Fierobe, H. P., Belaich, J. P., and Tardif, C. 2004. Towards designer cellulosomes in Clostridia: mannanase enrichment of the cellulosomes produced by Clostridium cellulolyticum. Journal of bacteriology. 186(19):6544-6552.
Petkowicza, C. L de O., Reicher, F., Chanzy, H., Taravel, F. R., and Vuong, R. 2001. Linear mannan in the endosperm of Schizolobium amazonicum. Carbohydrate Polymers. 44(2):107-112.
Pettey, L. A., Carter, S. D., Senne, B. W., and iver, J. A. 2002. Effects of beta-mannanase addition to corn-soybean meal diets on growth performance, carcass traits, and nutrient digestibility of weanling and growing-finishing pigs. Journal of Animal Science. 80(4):1012-1019.
Politz, O., Krah, M., Thomsen, K. K., and Borriss, R. 2000. A highly thermostable endo-(1, 4)-β-mannanase from the marine bacterium Rhodothermus marinus. Applied Microbiology and Biotechnology. 53(6):715-721.
Popa, V. I. and Spiridon, J. 1998. Hemicelluloses: structure and properties. In: Dumitriu S (ed) Polysaccharides: structural diversity and functional versatility. Marcel Dekker, New York, pp 297–311
Pucharta, V., Vršanskaa, M., Svobodab, P., Pohlb, J., Ogelc Z. B., and Bielya, P. 2004. Purification and characterization of two forms of endo-β-1,4-mannanase from a thermotolerant fungus, Aspergillus fumigatus IMI 385708 (formerly Thermomyces lanuginosus IMI 158749). Biochimica et Biophysica Acta (BBA) - General Subjects. 1674 (3):239-250.
Puls, J., and Schuseil, J. 1993. Chemistry of hemicelluloses : relationship between hemicellulose structure and enzymes required for hydrolysis. Hemicellulose and Hemicellulases. 8:1-27
Ramos, S., and Garcia Acha, I. 1975. A vegetative cycle of Pullularia pullulans. Transactions of the British Mycological Society. 64(1):129-135.
Regalado, C., Garcia‐Almendarez, B. E., Venegas‐Barrera, L. M., Tellez‐Jurado, A., Rodriguez‐Serrano, G., Huerta‐Ochoa, S., and Whitaker, J. R. 2000. Production, partial purification and properties of β‐mannanases obtained by solid substrate fermentation of spent soluble coffee wastes and copra paste using Aspergillus oryzae and Aspergillus niger. Journal of the Science of Food and Agriculture. 80(9):1343-1350.
Rho, D., Mulchandani, A., Luong, J. H., and LeDuy, A. 1988. Oxygen requirement in pullulan fermentation. Applied Microbiology and Biotechnology. 28(4-5):361-366.
Rinaldi, V. E. A., Ng, P. K. W., and Bennink, M. R. 2000. Effects of extrusion on dietary fibre and isoflavone contents of wheat extrudates enriched with wet okara. Cereal Chemistry. 77(2):237–240.
Sachslehner, A., Nidetzky, B., Kulbe, K. D. and Haltrich, D. 1998. Induction of mannanase, xylanase, and endoglucanase activities in Sclerotium rolfsii. Applied and Environmental Microbiology.64(2):594-600.
Sachslehner, A., Foidl, G., Foidl, N., Gubitz, G., and Haltrich, D. 2000. Hydrolysis of isolated coffee mannan and coffee extract by mannanases of Sclerotium rolfsii. Journal of Biotechnology. 80(2):127-134.
Saittagaroon, S., Kawakishi, S., and Namiki, M. 1983. Characterisation of polysaccharides of copra meal. Journal of the Science of Food and Agriculture. 34(8):855-860.
Scott, W. O., and Aldrich, S. R. 1983. Modern Soybean Production. S & A Publication. Champaign. IL. P 209 .
Shallom, D., and Shoham, Y. 2003. Microbial hemicellulases. Current Opinion in Microbiology. 6(3):219-228.
Shimizu, H. I. R. O. S. H. I. 2002. Manufacture of water-soluble soybean polysaccharide from soybean food by-product (okara). Foods and Food Ingredients Journal of Japan. 58-63.
Shingel, K. I. 2004. Current knowledge on biosynthesis, biological activity, and chemical modification of the exopolysaccharide pullulan. Carbohydrate Research. 339(3):447–460.
Singh, R. S., Saini, G. K. and Kennedy, J. F. 2008. Pullulan: microbial sources, production and applications. Carbohydrate Polymers. 73(4):515–531
Singleton, P., and Sainsburg, D. 1988. Dictionary of Microbology and Molecular Biology. 2nd ed. P. 413-504. John Wiley and Sons, Singapore.
Spilburg, C. A., Goldberg, A. C., McGill, J. B, Stenson, W. F., Racette, S. B., Bateman, J., McPherson, T. B., and Ostlund Jr, R. E. 2003. Fat-free foods supplemented with soy stanol-lecithin powder reduce cholesterol absorption and LDL cholesterol. Journal of the American Dietetic Association. 103(5):577-581
Sugiyama, N., Shimahara, H., Andoh, T., and Takamoto, M. 1973. Mannan and related compounds. II. Konjac-mannanase from the tubers of Amorphophallus konjac. Agricultural Biology and Chemistry. 1:9-17.
Sumida, M., Yuhki, T., Chen, R., Mori, H., Imamura, T. and Matsubara, F. 1995. Aseptic rearing of original silkworm strains on an artificial diet throughout the entire larval instars. Journal Sericult Science Japan. 64(1):35-38.
Sun, C. W., Chen, Z. W., He, Z. G., Zhou, P. J., and Liu, S. J. 2003. Purification and properties of the sulfur oxygenase/reductase from the acidothermophilic archaeon, Acidianus strain S5. Extremophiles. 7(2):131-134.
Sundu, B., Kumar, A., and Dingle, J. 2006. Response of broiler chicks fed increasing levels of copra meal and enzymes. International Journal of Poultry Science. 5(1):13-18.
Sutherland, I.W. 1998. Novel and established application of microbial polysaccharide. Trends in Biotechnologyl. 16(1):41-46 .
Tadano, T., Yokoya, K. and Hirokawa, R.1981. Studies on pneumatic drying of “okara”. Food Science and Technology. 83(5):478-482.
Talbot, G., and Sygusch, J. 1990. Purification and characterization of thermostable β-mannanase and α-galactosidase from Bacillus stearothermophilus. Applied and Environmental Microbiology. 56(11):3505-3510.
Timell, T. E. 1965. Wood Hemicelluloses: part II. Carbohydrate Chemistry. 20:409-483.
Titapoka, S., Keawsompong, S., Haltrich, D., and Nitisinprasert, S. 2008. Selection and characterization of mannanase-producing bacteria useful for the formation of prebiotic manno-oligosaccharides from copra meal. World Journal of Microbiology and Biotechnology. 24(8):1425-1433.
Toorop, P. E., Bewley, J. D., and Hilhorst, H. W. 1996. Endo-β-mannanase isoforms are present in the endosperm and embryo of tomato seeds, but are not essentially linked to the completion of germination. Planta. 200(2):153-158.
Tsukagoshi, N., Kobayashi, T., and Kato, M. 2001. Regulation of the amylolytic and (hemi-) cellulolytic genes in aspergilli. The Journal of General and Applied Microbiology. 47(1):1-19.
Turner, P., Mamo, G., and Karlsson, E. N. 2007. Potential and utilization of thermophiles and thermostable enzymes in biorefining. Microbial Cell Factories. 6(9): 1-23.
Tuohy, M. G., Buckley, R. J., Griffin, T. O., Connelly, I. C., Shanley Filho, N. A., EXF, H. M., and Coughlan, M. P. 1989. Enzyme production by solid-state cultures of aerobic fungi on lignocellulosic substrates. Enzyme systems for lignocellulose degradation. Elsevier, London. 293-312.
Urzi, C., De Leo, F., Lo, P. C., and Criseo, G. 1999. Intra-specific diversity of Aureobasidium pullulans strains isolated from rocks and other habitats assessed by physiological methods and by random amplified polymorphic DNA (RAPD). Journal of Microbiological Methods. 36(1-2):95-105.
Van der Riet, W. B., Wight, A. W., Cilliers, J. J. L., and Datel, J. M. 1989. Food chemical investigation of tofu and its byproduct okara. Food Chemistry. 34(3): 193-202.
Van Zyl, W. H., Rose, S. H., Trollope, K., and Gorgens, J. F. 2010. Fungal β-mannanases: mannan hydrolysis, heterologous production and biotechnological applications. Process Biochemistry. 45(8):1203-1213.
Wang, H. L., and Cavins, J. F. 1989. Yield and amino acid composition of fractions obtained during tofu production. Cereal Chemistry. 66(5):359-361.
Wang, L., Chi, Z. M., Wang, X. H., Liu, Z. Q., and Li, J. 2007. Diversity of lipase- producing yeasts from marine environments and oil hydrolysis by their crude enzymes. Annals of Microbiology. 57(4):495–501.
Watanabe, K., Kanoh, H., Yamanaka, S., Okiyama, A., and Kawanishi, T. 1997. Low calorie foodstuff, aqueous paste composition, as well as its production process thereof. U.S. Patent US 5690981, Chemistry Abstract. 128, 34079.
Watkins, J. T., Cantliffe, D. J., Huber, D. J., and Nell, T. A. 1985. Gibberellic acid stimulated degradation of endosperm in pepper. Journal of the American Society for Horticultural Science. 110:61-65.
Warren, R. A. J. 1996. Microbial hydrolysis of polysaccharides. Annual Reviews in Microbiology. 50(1):183-212.
Wolfrom, M. L., Laver, M. L., and Patin, D. L. 1961. Carbohydrates of the Coffee Bean. II. Isolation and Characterization of a Mannan. The Journal of Organic Chemistry. 26(11):4533-4535.
Wong, K. K., and Saddler, J. N. 1993. Applications of hemicellulases in the food, feed, and pulp and paper industries. Hemicellulose and Hemicellulases. 127-143.
Wong, K. K., Tan, L. U., and Saddler, J. N. 1988. Multiplicity of beta-1, 4-xylanase in microorganisms: functions and applications. Microbiological Reviews. 52(3), 305.
Woodward, J. 1984. Xylanases: functions, properties and applications. Topics in enzyme and fermentation biotechnology. 8:9-30.
Wozniewski, T., Blaschek, W., and Franz, G. 1992. Isolation and characterization of an endo-β-mannanase of Lilium testaceum bulbs. Phytochemistry. 31(10):3365-3370.
Wu, G., Bryant, M. M., Voitle, R. A., and Roland, D. A. 2005. Effects of β-Mannanase in corn-soy diets on commercial leghorns in second-cycle hens. Poultry Science. 84(6):894-897.
Xu, B., Hagglund, P., Stalbrand, H., and Janson, J. C. 2002. endo-β-1, 4-Mannanases from blue mussel, Mytilus edulis: purification, characterization, and mode of action. Journal of Biotechnology. 92(3):267-277.
Yoshii, H., Furuta, T., Maeda, H., and Mori, H. 1996. Hydrolysis kinetics of okara and characterization of its water-soluble polysaccharides. Bioscience, Biotechnology, and Biochemistry. 60(9):1406-1409.
Yuen, S. 1974. Pullulan and its applications. Process Biochemistry. 9:7–9.
Yurlova, N. A., and De Hoog, G. S. 1997. A new variety of Aureobasidium pullulans characterized by exopolysaccharide structure, nutritional physiology and molecular features. Antonie van leeuwenhoek, 72(2):141-147.
Zakaria, M. M., Ashiuchi, M., Yamamoto, S., and Yagi, T. 1998. Optimization for β-mannanase production of a psychrophilic bacterium, Flavobacterium sp. Bioscience, Biotechnology, and Biochemistry. 62(4):655-660.
Zhang, Q., Yan, X., Zhang, L., amd Tang, W. 2006. Cloning, sequence analysis, and heterologous expression of a β-mannanase gene from Bacillus subtilis Z-2. Molecular Biology. 40(3):368-374.
Zhang, M., Chen, X. L., Zhang, Z. H., Sun, C. Y., Chen, L. L., He, H. L., and Zhang, Y. Z. 2009. Purification and functional characterization of endo-β-mannanase MAN5 and its application in oligosaccharide production from konjac flour. Applied Microbiology and Biotechnology. 83(5):865-873.
Zhou, H., Zhao, W., and Wu, Y. 2008. Review About the Diversity of Mannanase. Biotechnology Bulletin. 2:017.


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