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研究生:鍾智堯
研究生(外文):Chih-Yao Chung
論文名稱:可食性菊科植物防治球蟲症的新穎機制
論文名稱(外文):A novel anticoccidial mechanism of edible Asteraceae herbs in chickens
指導教授:張力天
指導教授(外文):Lee-Tian Chang
口試委員:楊文欽張銘煌
口試委員(外文):Wen-Chin YangMing-Huang Chang
口試日期:2013-06-24
學位類別:碩士
校院名稱:國立中興大學
系所名稱:獸醫學系所
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:58
中文關鍵詞:雞球蟲症艾美球蟲屬家禽飼料添加物
外文關鍵詞:coccidiaEimeriapoultryfeed additive
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雞球蟲症是家禽產業最重要的疾病,使用化學藥物防治球蟲症仍是主要策略但衍生的抗藥性與藥物殘留問題,使歐盟原預計於西元2013 年禁止使用球蟲藥作為飼料添加物。研究和發展新型且安全的抗球蟲添加劑,同時避免藥物殘留問題益顯重要。我們實驗室先前已經研究並證明,利用一菊科植物於飼料添加能改善雞隻死亡,體重流失與病變,減少球蟲卵囊排放;我們進而想要了解此菊科植物如何產生抗球蟲效果。在體外實驗觀察芽孢化,在活體內觀察其生活史與型態的變化,以及對宿主腸道型態及免疫之影響。結果顯示此菊科植物能抑制球蟲卵囊芽孢化,餵食此菊科植物飼料能縮短球蟲生活史,促進世代轉換,改善黏膜完整程度與減少炎症反應。利用致病力與免疫保護力試驗,進一步觀察此菊科植物飼料其對球蟲族群的影響,發現其增加早熟株的數量於雞隻排放之球蟲卵囊。據我們所知,這是嶄新的抗球蟲機制,而這樣的結果揭示了此菊科植物飼料在現場應用的極高潛力。
Coccidiosis caused by Eimeria spp. is the parasitic disease that has the greatest economic impact on the poultry industry; prophylactic chemotherapy is dominated strategies to control the disease. However, with the increasing concerns about drug resistance and residue, anticoccidial drugs as feed additives were prohibited in the EU on 2013. Thus, to develop alternatives capable of maintaining animal health without negative effects mentioned above is necessary. The anticoccidial effect of an Asteraceae plant contained feed (AP) has been demonstrated in our laboratory. We further investigated the anticoccidial mechanism(s) of the AP. The sporulation rate, life cycle and morphology of E. tenella; and the mucosal morphology and inflammatory response of chickens were observed. Consequently, the AP aqueous solution inhibits the sporulation of E. tenella; and the AP shortens the life cycle and accelerates the endogenous development of E. tenella. The mucosal morphology and inflammatory response of chickens with AP were ameliorated. Furthermore, the pathogenicity and immunogenicity assay display the AP increases the populations of precocious line. To our knowledge, it is a novel mechanism that of anti-coccidia. The results also indicated that AP has a great potential on field application.
中文摘要 ........................................................................................................................... I
ABSTRACT ....................................................................................................................II
目次................................................................................................................................ III
圖目次..............................................................................................................................V
表目次............................................................................................................................ VI
第一章 緒言...................................................................................................................1
第二章 文獻探討...........................................................................................................2
第一節 雞球蟲症........................................................................................................2
一、 雞球蟲症之重要性.......................................................................................2
二、 雞Eimeria 屬球蟲之分類與致病性............................................................2
三、 雞Eimeria 屬球蟲之生活史........................................................................3
四、 雞Eimeria 屬球蟲與宿主免疫反應............................................................4
第二節 雞球蟲症之防治............................................................................................5
一、 球蟲疫苗.......................................................................................................5
二、 抗球蟲藥物與其機制...................................................................................6
三、 菊科植物之應用...........................................................................................7
第三章 材料與方法.......................................................................................................9
第一節 球蟲來源、繼代與鑑定................................................................................9
一、 球蟲來源.......................................................................................................9
二、 繼代與收集...................................................................................................9
三、 球蟲鑑定.......................................................................................................9
第二節 雞隻感染試驗..............................................................................................10
一、 Prepatent time 與生活史觀察.....................................................................11
二、 Pathogenicity ...............................................................................................11
三、 Immunogenicity...........................................................................................12
第三節 球蟲卵囊芽孢化試驗..................................................................................12
第四節 觀察項目與方法..........................................................................................13
一、 增重率與飼料換肉率.................................................................................13
二、 每公克糞便之卵囊數.................................................................................13
三、 盲腸肉眼病變分數.....................................................................................13
四、 組織病理學檢查.........................................................................................14
第五節 統計分析......................................................................................................14
第四章 結果.................................................................................................................16
第一節 球蟲型態與PCR 鑑定................................................................................16
第二節 AP 水溶液抑制球蟲卵囊芽孢化................................................................16
第三節 球蟲生活史觀察..........................................................................................16
一、 Prepatent time 與O.P.G. .............................................................................16
二、 組織病理學檢查.........................................................................................17
第四節 PATHOGENICITY.............................................................................................18
第五節 IMMUNOGENICITY .........................................................................................18
第六節 腸道黏膜型態與炎症反應..........................................................................18
第五章 討論.................................................................................................................33
參考文獻.........................................................................................................................37
附圖.................................................................................................................................43
附表.................................................................................................................................52
Allen, P.C., 2003. Dietary supplementation with Echinacea and development of
immunity to challenge infection with coccidia. Parasitology Research 91, 74-78.
Allen, P.C., Fetterer, R.H., 2002. Recent Advances in Biology and Immunobiology of
Eimeria Species and in Diagnosis and Control of Infection with These Coccidian
Parasites of Poultry. Clinical Microbiology Reviews 15, 58-65.
Allen, P.C., Lydon, J., Danforth, H.D., 1997. Effects of components of Artemisia annua
on coccidia infections in chickens. Poultry Science 76, 1156-1163.
Asea, A., Kraeft, S.-K., Kurt-Jones, E.A., Stevenson, M.A., Chen, L.B., Finberg, R.W.,
Koo, G.C., Calderwood, S.K., 2000. HSP70 stimulates cytokine production
through a CD14-dependant pathway, demonstrating its dual role as a chaperone
and cytokine. Nature Medicine 6, 435-442.
Augustine, P.C., McNaughton, J.L., Virtanen, E., Rosi, L., 1997. Effect of betaine on the
growth performance of chicks inoculated with mixed cultures of avian Eimeria
species and on invasion and development of Eimeria tenella and Eimeria
acervulina in vitro and in vivo. Poultry Science 76, 802-809.
Baba, E., Wakeshima, H., Fukui, K., Fukata, T., Arakawa, A., 1992. Adhesion of
bacteria to the cecal mucosal surface of conventional and germ-free chickens
infected with Eimeria tenella. American Journal of Veterinary Research 53,
194-197.
Blake, D.P., Qin, Z., Cai, J., Smith, A.L., 2008. Development and validation of real-time
polymerase chain reaction assays specific to four species of Eimeria. Avian
Pathology 37, 89-94.
Castanon, C.A.B., Fraga, J.S., Fernandez, S., Gruber, A., da F. Costa, L., 2007.
Biological shape characterization for automatic image recognition and diagnosis
of protozoan parasites of the genus Eimeria. Pattern Recognition 40, 1899-1910.
Chapman, H.D., 1997. Biochemical, genetic and applied aspects of drug resistance in
Eimeria parasites of the fowl. Avian Pathology 26, 221-244.
Chapman, H.D., 1998. Evaluation of the efficacy of anticoccidial drugs against Eimeria
species in the fowl. International Journal for Parasitology 28, 1141-1144.
Collier, C.T., Hofacre, C.L., Payne, A.M., Anderson, D.B., Kaiser, P., Mackie, R.I.,
Gaskins, H.R., 2008. Coccidia-induced mucogenesis promotes the onset of
necrotic enteritis by supporting Clostridium perfringens growth. Veterinary
Immunology and Immunopathology 122, 104-115.
Constantinoiu, C.C., Molloy, J.B., Jorgensen, W.K., Coleman, G.T., 2011.
Characterization of the antibody response in birds following infection with
wild-type and attenuated strains of Eimeria tenella and Eimeria necatrix.
Veterinary Parasitology 175, 47-51.
Conway, D.P., McKenzie, M.E., 2007. Poultry coccidiosis: Diagnostic and testing
procedures. Blackwell Pub.
Cooper, H.S., Murthy, S.N., Shah, R.S., Sedergran, D.J., 1993. Clinicopathologic study
of dextran sulfate sodium experimental murine colitis. Laboratory Investigation;
a Journal of Technical Methods and Pathology 69, 238-249.
Dalloul, R.A., Lillehoj, H.S., 2006. Poultry coccidiosis: recent advancements in control
measures and vaccine development. Expert Review of Vaccines 5, 143-163.
Daszak, P., 1999. Secretion of multilamellar whorls by Eimeria tenella zoites. The
Journal of Parasitology, 742-746.
del Cacho, E., Gallego, M., Francesch, M., Quilez, J., Sanchez-Acedo, C., 2010. Effect
of artemisinin on oocyst wall formation and sporulation during Eimeria tenella
infection. Parasitology International 59, 506-511.
del Cacho, E., Gallego, M., Lopez-Bernad, F., Quilez, J., Sanchez-Acedo, C., 2005.
Differences in hsp70 expression in the sporozoites of the original strain and
precocious lines of Eimeria tenella. Journal of Parasitology 91, 1127-1131.
del Cacho, E., Gallego, M., Pages, M., Monteagudo, L., Sanchez-Acedo, C., 2006.
Effect of the quinolone coccidiostat decoquinate on the rearrangement of
chromosomes of Eimeria tenella. International Journal for Parasitology 36,
1515-1520.
del Cacho, E., Gallego, M., Pages, M., Monteagudo, L., Sanchez-Acedo, C., 2008.
HSP70 is part of the synaptonemal complex in Eimeria tenella. Parasitology
International 57, 454-459.
Dobbin, C.A., Smith, N.C., Johnson, A.M., 2002. Heat shock protein 70 is a potential
virulence factor in murine Toxoplasma infection via immunomodulation of host
NF-κB and nitric oxide. The Journal of Immunology 169, 958-965.
Dong, H., Lin, J., Han, H., Jiang, L., Zhao, Q., Zhu, S., Huang, B., 2011. Analysis of
differentially expressed genes in the precocious line of Eimeria maxima and its
parent strain using suppression subtractive hybridization and cDNA microarrays.
Parasitology Research 108, 1033-1040.
Dong, H., Suo, X., Wang, M., Teng, K., 2006. Characteristics of a line of eimeria
necatrix after 16 successive passages of oocysts collected after peak oocyst
production. Journal of Parasitology 92, 1229-1234.
Elmusharaf, M., Bautista, V., Nollet, L., Beynen, A., 2006. Effect of a
mannanoligosaccharide preparation on Eimeria tenella infection in broiler
chickens. International Journal of Poultry Science 5, 583-588.
Georgieva, N., Koinarski, V., Gadjeva, V., 2006. Antioxidant status during the course of
Eimeria tenella infection in broiler chickens. The Veterinary Journal 172,
488-492.
Guo, F.C., Suo, X., Zhang, G.Z., Shen, J.Z., 2007. Efficacy of decoquinate against drug
sensitive laboratory strains of Eimeria tenella and field isolates of Eimeria spp.
in broiler chickens in China. Veterinary Parasitology 147, 239-245.
Haug, A., Williams, R.B., Larsen, S., 2006. Counting coccidial oocysts in chicken
faeces: a comparative study of a standard McMaster technique and a new rapid
method. Veterinary Parasitology 136, 233-242.
Hirpara, K.V., Aggarwal, P., Mukherjee, A.J., Joshi, N., Burman, A.C., 2009. Quercetin
and its derivatives: synthesis, pharmacological uses with special emphasis on
anti-tumor properties and prodrug with enhanced bio-availability. Anti-Cancer
Agents in Medicinal Chemistry 9, 138-161.
Hodgson, J.N., 1970. Coccidiosis: oocyst counting technique for coccidiostat evaluation.
Experimental Parasitology 28, 99-102.
Hume, M.E., Barbosa, N.A., Dowd, S.E., Sakomura, N.K., Nalian, A.G.,
Martynova-Van Kley, A., Oviedo-Rondon, E.O., 2011. Use of pyrosequencing
and denaturing gradient gel electrophoresis to examine the effects of probiotics
and essential oil blends on digestive microflora in broilers under mixed Eimeria
infection. Foodborne Pathogens and Disease 8, 1159-1167.
Hwang, I.-Y., Quan, J.H., Ahn, M.-H., Ahmed, H.A.H., Cha, G.-H., Shin, D.-W., Lee,
Y.-H., 2010. Toxoplasma gondii infection inhibits the mitochondrial apoptosis
through induction of Bcl-2 and HSP70. Parasitology Research 107, 1313-1321.
Jeffers, T.K., 1975. Attenuation of Eimeria tenella through selection for precociousness.
The Journal of Parasitology 61, 1083-1090.
Jeurissen, S.H.M., Janse, E.M., Vermeulen, A.N., Vervelde, L., 1996. Eimeria tenella
infections in chickens: aspects of host-parasite: interaction. Veterinary
Immunology and Immunopathology 54, 231-238.
Johnson, J.K., Long, P.L., McKenzie, M.E., 1986b. The pathogenicity, immunogenicity
and endogenous development of a precocious line of Eimeria brunetti. Avian
Pathology 15, 697-704.
Kawazoe, U., Bordin, E.L., de Lima, C.A., Dias, L.A., 2005a. Characterisation and
histopathological observations of a selected Brazilian precocious line of Eimeria
acervulina. Veterinary Parasitology 131, 5-14.
Kaya, G., Dale, C., Maudlin, I., Morgan, K., 2007. A novel procedure for total nucleic
acid extraction from small numbers of Eimeria species oocysts. Turkiye
parazitolojii dergisi / Turkiye Parazitoloji Dernegi = Acta parasitologica Turcica
/ Turkish Society for Parasitology 31, 180-183.
Kennedy, D.G., Blanchflower, W.J., O'Dornan, B.C., 1997. Development of an ELISA
for maduramicin and determination of the depletion kinetics of maduramicin
residues in poultry. Food Additives; Contaminants 14, 27-33.
Kitandu, A., Juranova, R., 2006. Progress in control measures for chicken coccidiosis.
Acta Veterinaria BRNO 75, 265-276.
Lee, J.T., Eckert, N.H., Ameiss, K.A., Stevens, S.M., Anderson, P.N., Anderson, S.M.,
Barri, A., McElroy, A.P., Danforth, H.D., Caldwell, D.J., 2011. The effect of
dietary protein level on performance characteristics of coccidiosis vaccinated
and nonvaccinated broilers following mixed-species Eimeria challenge. Poultry
Science 90, 1916-1925.
Leiriao, P., Mota, M.M., Rodriguez, A., 2005. Apoptotic Plasmodium-infected
hepatocytes provide antigens to liver dendritic cells. Journal of Infectious
Diseases 191, 1576-1581.
Levine, N.D., 1985. Species of the Coccidian Genus Alveocystis1. Journal of
Eukaryotic Microbiology 32, 368-369.
Li, G.Q., Kanu, S., Xiang, F.Y., Xiao, S.M., Zhang, L., Chen, H.W., Ye, H.J., 2004.
Isolation and selection of ionophore-tolerant Eimeria precocious lines: E. tenella,
E. maxima and E. acervulina. Veterinary parasitology 119, 261-276.
Li, J., Xing, T., Wang, L., Tao, J., Liu, Z., 2010. Inhibitory effect of
S-nitroso-glutathione on Eimeria tenella oocysts was mainly limited to the early
stages of sporogony. Veterinary Parasitology 173, 64-69.
Lillehoj, H., Min, W., Dalloul, R., 2004. Recent progress on the cytokine regulation of
intestinal immune responses to Eimeria. Poultry Science 83, 611-623.
Lillehoj, H.S., 1998. Role of T lymphocytes and cytokines in coccidiosis. International
Journal for Parasitology 28, 1071-1081.
Lillehoj, H.S., Trout, J.M., 1996. Avian gut-associated lymphoid tissues and intestinal
immune responses to Eimeria parasites. Clinical Microbiology Reviews 9,
349-360.
Long, P.L., 1970. Anticoccidial drugs: factors affecting pathogenicity of avian coccidia.
Experimental parasitology 28, 4-10.
Martynova-VanKley, A., Syvyk, A., Teplova, I., Hume, M., Nalian, A., 2008. Rapid
Detection of Avian Eimeria Species Using Denaturing Gradient Gel
Electrophoresis. Poultry Science 87, 1707-1713.
McDonald, V., Ballingall, S., 1983a. Attenuation of Eimeria mivati (= mitis) by
selection for precocious development. Parasitology 86, 371-379.
McDonald, V., Shirley, M., 1984. Eimeria mitis: a comparison of the endogenous
developmental stages of a line selected for early maturation and the parent strain.
Parasitology 88, 37-44.
McDonald, V., Shirley, M.W., 2009. Past and future: vaccination against Eimeria.
Parasitology 136, 1477-1489.
McDougald, L., 1998. Intestinal protozoa important to poultry. Poultry Science 77,
1156-1158.
Michels, M.G., Bertolini, L.C., Esteves, A.F., Moreira, P., Franca, S.C., 2011.
Anticoccidial effects of coumestans from Eclipta alba for sustainable control of
Eimeria tenella parasitosis in poultry production. Veterinary Parasitology 177,
55-60.
Molan, A.L., ZhuoJian, L., ShamPa, D., 2009. Effect of pine bark (Pinus radiata)
extracts on sporulation of coccidian oocysts. Folia Parasitologica 56, 1-5.
Montes, C., Rojo, F., Hidalgo, R., Ferre, I., Badiola, C., 1998. Selection and
development of a Spanish precocious strain of Eimeria necatrix. Veterinary
Parasitology 78, 169-183.
Motard, A., Marussig, M., Renia, L., Baccam, D., Landau, I., Mattei, D., Targett, G.,
Mazier, D., 1995. Immunization with the malaria heat shock like protein hsp70-1
enhances transmission to the mosquito. International Immunology 7, 147-150.
Mouafo, A., Weck-Heimann, A., Dubremetz, J.-F., Entzeroth, R., 2002. Monoclonal
antibodies specific for the two types of wall-forming bodies of Eimeria tenella
macrogametes (Coccidia, Apicomplexa). Parasitology Research 88, 217-224.
Mun, H.-S., Aosai, F., Norose, K., Chen, M., Hata, H., Tagawa, Y.-i., Iwakura, Y., Byun,
D.-S., Yano, A., 2000. Toxoplasma gondii Hsp70 as a danger signal in
Toxoplasma gondii-infected mice. Cell stress & chaperones 5, 328.
Naidoo, V., McGaw, L.J., Bisschop, S.P., Duncan, N., Eloff, J., 2008. The value of plant
extracts with antioxidant activity in attenuating coccidiosis in broiler chickens.
Veterinary Parasitology 153, 214-219.
Nweze, N.E., Obiwulu, I.S., 2009. Anticoccidial effects of Ageratum conyzoides.
Journal of Ethnopharmacology 122, 6-9.
Oviedo-Rondon, E., Clemente-Hernandez, S., Salvador, F., Williams, P., Losa, R., 2006.
Essential oils on mixed coccidia vaccination and infection in broilers.
International Journal of Poultry Science 5, 723-730.
Ovington, K.S., Alleva, L.M., Kerr, E.A., 1995. Cytokines and immunological control
of Eimeria spp. International Journal for Parasitology 25, 1331-1351.
Pakandl, M., Jelinkova, A., 2006. The rabbit coccidium Eimeria piriformis: Selection of
a precocious line and life-cycle study. Veterinary Parasitology 137, 351-354.
Peek, H.W., Landman, W.J.M., 2006. Higher Incidence of Eimeria spp. Field Isolates
Sensitive for Diclazuril and Monensin Associated with the Use of Live
Coccidiosis Vaccination with Paracox™-5 in Broiler Farms. Avian Diseases 50,
434-439.
Peek, H.W., Landman, W.J.M., 2011. Coccidiosis in poultry: anticoccidial products,
vaccines and other prevention strategies. Veterinary Quarterly 31, 143-161.
Pinard-Van Der Laan, M.H., Monvoisin, J.L., Pery, P., Hamet, N., Thomas, M., 1998.
Comparison of outbred lines of chickens for resistance to experimental infection
with coccidiosis (Eimeria tenella). Poultry Science 77, 185-191.
Qin, Z.R., fukata, T., Baba, E., Arakawa, A., 1995. Effect of Eimeria tenella infection
on Salmonella enteritidis infection in chickens. Poultry Science 74, 1-7.
Sharma, N., Bhalla, A., Varma, S., Jain, S., Singh, S., 2005. Toxicity of maduramicin.
Emergency Medicine Journal 22, 880-882.
Sharman, P.A., Smith, N.C., Wallach, M.G., Katrib, M., 2010. Chasing the golden egg:
vaccination against poultry coccidiosis. Parasite Immunology 32, 590-598.
Shirley, M.W., Bedrnik, P., 1997. Live attenuated vaccines against avian coccidiosis:
Success with precocious and egg-adapted lines of Eimeria. Parasitology Today
13, 481-484.
Shirley, M.W., McDonald, V., Chapman, H.D., Millard, B.J., 1984. Eimeria praecox:
selection and characteristics of precocious lines. Avian Pathology 13, 669-682.
Shirley, M.W., Smith, A.L., Blake, D.P., 2007. Challenges in the successful control of
the avian coccidia. Vaccine 25, 5540-5547.
Silva, N.M., Gazzinelli, R.T., Silva, D.A.O., Ferro, E.A.V., Kasper, L.H., Mineo, J.R.,
1998. Expression of Toxoplasma gondii-specific heat shock protein 70 during in
vivo conversion of bradyzoites to tachyzoites. Infection and Immunity 66,
3959-3963.
Tomley, F., 1997. Techniques for isolation and characterization of apical organelles from
Eimeria tenella sporozoites. Methods (San Diego, Calif.) 13, 171-176.
Truman, A.W., Kristjansdottir, K., Wolfgeher, D., Hasin, N., Polier, S., Zhang, H.,
Perrett, S., Prodromou, C., Jones, G.W., Kron, S.J., 2012. CDK-dependent
Hsp70 phosphorylation controls G1 cyclin abundance and cell-cycle progression.
Cell 151, 1308-1318.
Wallach, M., 2010. Role of antibody in immunity and control of chicken coccidiosis.
Trends in Parasitology 26, 382-387.
Wallach, M.G., Ashash, U., Michael, A., Smith, N.C., 2008. Field application of a
subunit vaccine against an enteric protozoan disease. PloS one 3, e3948.
Weiss, L.M., Ma, Y.F., Takvorian, P.M., Tanowitz, H.B., Wittner, M., 1998. Bradyzoite
Development in Toxoplasma gondii and the hsp70 Stress Response. Infection
and Immunity 66, 3295-3302.
White, N.J., 2004. Antimalarial drug resistance. Journal of Clinical Investigation 113,
1084-1092.
Willcox, M.L., Bodeker, G., 2004. Traditional herbal medicines for malaria. BMJ:
British Medical Journal 329, 1156.
Williams, R.B., 1999. A compartmentalised model for the estimation of the cost of
coccidiosis to the world's chicken production industry. International Journal for
Parasitology 29, 1209-1229.
Williams, R.B., 2002. Anticoccidial vaccines for broiler chickens: Pathways to success.
Avian Pathology 31, 317-353.
Williams, R.B., 2006. Tracing the emergence of drug-resistance in coccidia (Eimeria
spp.) of commercial broiler flocks medicated with decoquinate for the first time
in the United Kingdom. Veterinary Parasitology 135, 1-14.
Williams, R.B., 2007. “Serotinous”, a descriptor proposed for lines of Eimeria species
(Apicomplexa: Eimeriidae) selected for delayed development: an antonym for
“precocious”. Veterinary Parasitology 145, 388-389.
Youn, H.J., Noh, J.W., 2001. Screening of the anticoccidial effects of herb extracts
against Eimeria tenella. Veterinary Parasitology 96, 257-263.
Yun, C.H., Lillehoj, H.S., Lillehoj, E.P., 2000. Intestinal immune responses to
coccidiosis. Developmental & Comparative Immunology 24, 303-324.
Zhang, L., Ma, L., Liu, R., Zhang, Y., Zhang, S., Hu, C., Song, M., Cai, J., Wang, M.,
2012. Eimeria tenella heat shock protein 70 enhances protection of recombinant
microneme protein MIC2 subunit antigen vaccination against E. tenella
challenge. Veterinary Parasitology.
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