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研究生:陳博淵
研究生(外文):Po-YungChen
論文名稱:雌二醇衍生物探針之製備及其與雌激素受體鍵結分析
論文名稱(外文):Fabrication of Estradiol Chemical Probes and the Study of Their Estrogen Receptor Binding
指導教授:陳淑慧陳淑慧引用關係
指導教授(外文):Shu-Hui Chen
學位類別:碩士
校院名稱:國立成功大學
系所名稱:化學系碩博士班
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:76
中文關鍵詞:雌激素雌激素受體鍵結
外文關鍵詞:EstrogenEstrogen receptorBinding
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雌激素為人體內的一種荷爾蒙,男性女性均會分泌,會影響人體的生長,例如女性進入青春期或是懷孕期,近年來研究發現雌激素會促進乳癌細胞MCF-7的表現,表現過度會產生癌化現象,目前許多科學家朝向雌激素與雌激素受體之間的鍵結親和力或是其他也可能與雌激素有親和力的蛋白質做研究。
本論文使用化學合成的方式合成雌激素衍生物,之後對合成出的雌激素衍生物進行相對鍵結親和力測試,再選用與雌激素受體有高度親和力的衍生物搭配蛋白質體學的研究方式,使用金奈米粒子進行免疫沉澱方法,以此方法成功捕捉及純化目標蛋白質,例如雌激素受體等,日後可應用至找尋雌激素受體如何誘發乳癌的途徑。
Estrogens are kinds of hormones secreted by both male and female. For example, estrogens would influence the growth of the human body during the period of puberty and pregnancy. Recently, many studies showed that estrogens would promote the expression of breast cancer cell line MCF-7. When it over express that would produce cancerous phenomenon. For this reason, many scientists studied the binding affinity between estrogen and estrogen receptors or other proteins which would also influence the affinity of estrogens.
This work aims to synthesize estrogen derivatives by chemical synthesis. For the analysis of relative binding affinity of the estrogen derivatives, we utilized the ligand binding assay to study. According to the result of the relative binding affinity data, we choose the highly relative binding affinity estrogen derivatives. We attempted the strategy of immunoprecipitation with gold nanoparticles in order to successfully catch and purify targeted protein such as estrogen receptor alpha ( ERα ). Further, this method will probably find application in identifying the pathway of breast cancer with ERα expression.

摘要                       i
Abstract ii
謝誌 iii
目錄 iv
圖目錄 vi
表目錄 vii
流程目錄 vii
英文縮寫對照表 viii
第一章:序論 1
第一節、乳癌、雌激素與雌激素受體 1
第二節、螢光共振能量轉移與配位基競爭反應 13
第三節、蛋白質體學與質譜 18
第四節、質譜學 21
第五節、蛋白質體學與質譜 29
第六節、生物醫學以及奈米科技 31
第二章:結果與討論 33
第一節、雌二醇的衍生化合成 33
第二節、衍生物與受體的相對鍵結親和力分析 37
第三節、雌激素衍生物在免疫沉澱的應用 44
第四節、結論 51
第三章:實驗部分與參考資料 53
第一節、一般實驗方法 53
第二節、化合物合成實驗方法及光譜資料 54
第三節、配位基競爭反應實驗方法 57
第四節、免疫沉澱實驗方法 60
參考文獻 63
附錄 67

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