臺灣博碩士論文加值系統

治療惡性腫瘤最常見的方法為放射性療法、化學療法和手術治療，雖然三種治療方式發展，在提高患者生存率上有一定的進步，但是仍不能取得突破性的進展，並且對人體正常組織產生較大的副作用。積極探索更有效的治療手段是目前腫瘤的治療方向。已有動物實驗和臨床觀察研究證明，磁場能誘導腫瘤細胞的抑制或殺滅腫瘤組織，使得磁場有望成為腫瘤治療的一種新手段，磁場結合藥物，成為磁場和磁性材料應用於腫瘤治療的一種新的發展趨勢
本論文是利用亥姆霍茲線圈產生之不同脈衝電磁場以及抗癌藥物牛樟芝，並利用生物學上常用的細胞存活指標MTT assay，藉以觀測不同的低頻脈衝電磁場對細胞生長的影響，以及牛樟芝結合脈衝電磁場是否增加了抑制細胞的時間，實驗藉由將此脈衝電磁場放進一個長時間培養細胞的環境，並將溫度以及氣體的變異排除，才能真正觀察出脈衝電磁場對細胞的影響。在暴露於0.55mT、1.1mT、2.2mT磁場下，癌細胞均有延遲生長的反應，但是不同的細胞對於磁場有不同的適應力，黑色素瘤細胞在1.1mT和牛樟芝作用下生長量為控制組的48%，而子宮頸癌細胞在0.55mT和牛樟芝的結合下生長量為控制組的49%。

In this study, Helmholtz coils utilized to generate pulse electromagnetic fields combine with anti-cancer drugs Antrodia camphorata, and using biological index MTT assay commonly used in determination of cell survival rate. The purpose is to observe the influence of low frequency pulse electromagnetic fields on tumor cell growth, as well as the combination of PEMF and Antrodia camphorata increase the retardation time of cell growth or not. This experiment put pulse electromagnetic fields system into a cell culture environment that the temperature and the gas variation are excluded. Therefore, we can really observe the effect of pulse electromagnetic fields on cells. After exposure to 0.55mT, 1.1mT, 2.2mT magnetic fields, the growth of cancer cells have retardation effect, but the different magnetic fields have dissimilar reaction. Melanoma cells under 1.1mT and Antrodia camphorata only have 48% cell growth rate of the control group. Hela cells under 0.55mT and Antrodia camphorata only have 49% cell growth of the control group.

中文摘要 I
ABSTRACT II
ACKNOWLEDGMENT III
CONTENTS IV
LIST OF FIGURES VI
CHAPTER 1 INTRODUCTION 1
11 Background...........................1
12 Theory of magnetic stimulation .................1
13 Biological Effects of Electromagnetic Fields..............2
14 Reference about cell with PEMF stimulation ..............4
15 Motivation............................5
CHAPTER 2 MATERIAL AND METHOD 6
21 Introduction............................6
211 Cell Line 6
212 Taiwanofungus camphoratus 6
213 Helmholtz coil 7
22 PEMF Design...........................8
23 MTT assay...........................10
24 Cell Analysis...........................11
25 Optical density value.......................12
CHAPTER 3 EXPERIMENTAL SETUP 14
31 Experimental setup........................14
32 Exciting condition.........................15
321 The Pulse Electromagnetic Fields on growth of tumor cells 15
322 The Antrodia camphorata on growth of tumor cells 15
323 The Antrodia camphorata with PEMFs on growth of tumor cells 15
CHAPTER 4 EXPERIMENTAL RESULTS 17
41 The results of 055 mT on HeLa cell.................17
42 The results of 11 mT on HeLa cell..................18
43 The results of 22mT on HeLa cell..................19
44 The results of 055 mT on Melanoma cell...............20
45 The results of 11 mT on Melanoma cell................21
46 The results of 22 mT on Melanoma cell................22
CHAPTER 5 DISCUSSION 24
CHAPTER 6 CONCLUSION 38
References....................39

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