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研究生:黃紋佩
研究生(外文):Wen-Pei huang
論文名稱:南瓜果實酒精抽取物抗疲勞功效評估
論文名稱(外文):Anti-fatigue effect of an ethanolic extract from Cucurbita moschata
指導教授:黃啟彰黃啟彰引用關係
指導教授(外文):Chi-Chang Huang
學位類別:碩士
校院名稱:國立體育大學
系所名稱:運動科學研究所
學門:民生學門
學類:運動科技學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:48
中文關鍵詞:南瓜抗疲勞運動表現肝醣乳酸血氨肌酸激酶
外文關鍵詞:Pumpkinanti-fatigueexercise performanceglycogenlactic acidammoniacreatine kinase
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南瓜果實酒精抽取物抗疲勞功效評估

摘要
南瓜(Cucurbita moschata)為世界著名且分佈相當普遍之食材,是夏秋季節瓜菜之一,作為菜餚、甜點等。南瓜成份含有豐富醣類與纖維質、少量蛋白質及脂質,及多種維生素等。本研究目的在探討補充南瓜果實酒精抽出物(C. moschata extract, CME)之抗疲勞功效。本實驗採用小鼠平台進行測試,將雄性ICR小鼠分作四組(每組8隻),透過經口投予方式餵食不同劑量之CME,劑量分別為:0、50、100和250 (毫克/公斤/天)。連續補充兩週後,分別針對運動生理表現和抗疲勞血液生化數據進行分析。測量項目包括:5%負重游泳力竭時間、前肢抓力以及無負重單次固定15分鐘游泳運動後的血乳酸、血氨、血糖濃度與肌酸激酶活性。此外,連續補充南瓜萃取物對於休息狀態下肌肉和肝臟組織部位的肝醣含量也同步作分析。本研究結果顯示,兩週補充CME具有提升前肢握力之效果。且補充CME劑量組在負重5%的游泳運動表現、血糖、肌肉和肝臟肝醣含量皆隨著補充劑量增加而具有顯著提升的效果。此外,補充CME組也具有明顯降低單次15分鐘游泳運動後的血乳酸與血氨濃度以及肌酸激酶活性。根據我們的實驗結果顯示,CME具有抗疲勞的效果可能與增加能量儲存(肝醣)和能量釋放(如血糖)、血漿中乳酸與血氨濃度減少以及肌酸激酶活性降低有關。故南瓜果實酒精抽取物極具開發成為抗疲勞食品與運動增補劑之應用潛力。

關鍵字:南瓜、抗疲勞、運動表現、肝醣、乳酸、血氨、肌酸激酶

Anti-fatigue effect of an ethanolic extract from Cucurbita moschata

Abstract
Pumpkin is one of the well-known ingredients in the world. It is a gourd-like squash of the genus Cucurbita and the family Cucurbitaceae by plant taxonomy, and is classified as one kind of the staple foods according to its nutritional value. Pumpkin is rich in carbohydrates, fiber and vitamins, and a little protein and lipid. The overall purpose of this study was to evaluate the effects of C. moschata extract (CME) on anti-fatigue and ergogenic functions following physiological challenges. Male ICR mice from four groups designated vehicle, CME-50, CME-100 and CME-250, respectively (n=8/group) were orally administered CME for 14 days at 0, 50, 100 and 250 mg/kg/day. The anti-fatigue activity and exercise performance were evaluated using exhaustive swimming time, forelimb grip strength, as well as levels of plasma lactate, ammonia, glucose, and creatine kinase after an acute swimming exercise. The resting muscular and hepatic glycogen was also analyzed after a 14-day supplementation with CME. Trend analysis revealed that CME treatments increased grip strength. CME dose-dependently increased 5% body weight loaded swimming time, blood glucose, and muscular and hepatic glycogen levels. CME dose-dependently decreased plasma lactate and ammonia levels and creatine kinase activity after a 15-min swimming test. The mechanism was relevant to the increase in energy storage (as glycogen) and release (as blood glucose), and the decrease of plasma levels of lactate, ammonia, and creatine kinase. Therefore, CME could be used for developing as an anti-fatigue agent or ergogenic aids.

Keywords: Pumpkin, anti-fatigue, exercise performance, glycogen, lactic acid, ammonia, creatine kinase

目錄
第壹章 緒論 1
第一節 研究背景 1
第二節 研究目的 2
第貳章 文獻探討 3
第一節 南瓜及其活性成分相關研究 3
第二節 運動與疲勞 6
第三節 相關營養增補劑研究 9
第四節 文獻小結 11
第參章 材料與方法 12
第一節 實驗測試樣品 12
第二節 實驗動物之飼養 13
第三節 實驗設計 14
第四節 血液與組織樣本之收集及前處理 20
第五節 統計分析 22
第肆章 結果 23
第一節 運動表現能力測試 23
第二節 血液生化指標乳酸、血氨,血糖濃度以及肌酸激酶活性之分析 25
第三節 肌肉與肝臟組織中肝醣含量之分析 27
第四節 組織切片之比較 28
第伍章 討論 29
第一節 補充南瓜果實酒精抽取物對運動表現的影響 29
第二節 補充南瓜果實酒精抽取物對血中乳酸、血氨、血糖的濃度以及 肌酸激酶活性的影響 30
第三節 補充南瓜果實酒精抽取物對肝臟與肌肉組織中肝醣含量的影響 32
第陸章 結論與建議 33
參考文獻 34


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