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研究生:張馨芳
研究生(外文):Hsin-Fang Chang
論文名稱:以原子力顯微鏡觀測白蛋白吸附層之微結構
論文名稱(外文):The Microstructure of Adsorbed Albumin Investigated by Atomic Force Microscopy
指導教授:楊屹沛楊屹沛引用關係
口試委員:蔡瑞瑩蔡孟利楊屹沛
口試日期:2013-07-23
學位類別:碩士
校院名稱:國立宜蘭大學
系所名稱:生物機電工程學系碩士班
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:62
中文關鍵詞:白蛋白原子力顯微術吸附
外文關鍵詞:AlbuminAtomic Force MicroscopyAdsorption
相關次數:
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  • 下載下載:6
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本研究目的是針對白蛋白於不同基材上之吸附現象進行探討。使用原子力顯微鏡觀測白蛋白吸附於雲母片(Mica)、聚甲基丙烯酸甲酯(PMMA)、聚苯乙烯(PS)之表面微結構形態,改變吸附時間及蛋白質溶液濃度,觀察其吸附微結構形態之變化。結果顯示,白蛋白吸附於親、疏水性基材上,會快速吸附至表面上並呈現飽和的穩態單層吸附,且此單層結構相當穩定,不受蛋白質溶液濃度增加、吸附時間延長的影響。在達到飽和吸附前,疏水性表面吸附白蛋白速率比親水性表面快;蛋白質溶液濃度增加也可增加白蛋白吸附速率。三種基材實驗中都出現了共通的不穩定現象,即被擠壓出表面而形成的突出顆粒,這些突出顆粒可能是白蛋白在吸附過程中所受小擾動而產生的一種暫穩結構。
The microstructure of albumin adsorbed on solid substrates has been investigated by atomic force microscopy. In this work, we utilized different substrates such as mica, PMMA, and PS as the supporting surface that the albumin molecules adsorption onto this surface was characterized. We also exploited the morphology of the adsorption albumin varied by the concentrations of albumin solutions and the adsorption time. These results indicate that albumin adsorbed rapidly onto the surface of both hydrophilic and hydrophobic substrates. Within a few minutes, the adsorption albumin molecules formed a saturated stable monolayer which appeared in all observations of the various concentrations and adsorption time. However, albumin adsorbed faster on the hydrophobic surface than on the hydrophilic surface before the saturation time of adsorption. The more concentrations of protein solutions also shortened the saturation time. For a saturation adsorbed albumin monolayer, the surface film consists of granular convex structure. Adsorption time experiments indicate that the formation of the granular convex domain is a metastable structure.
摘要 I
ABSTRACT II
目錄 III
圖目錄 V
表目錄 VI
第一章 緒論 1
第一節 白蛋白介紹 2
第二節 文獻探討 4
第三節 研究目的 8
第二章 材料與方法 9
第一節 實驗藥品 9
第二節 實驗器材 11
第三節 實驗儀器 13
第四節 實驗方法 15
2-4-1 配製磷酸緩衝溶液 15
2-4-2 配製 PMMA、PS 溶液 15
2-4-3 製備 PMMA 及 PS 高分子薄膜基材 16
2-4-4 白蛋白溶液配製 17
2-4-5 製備不同濃度、不同吸附時間之白蛋白吸附層樣品 17
2-4-6 原子力顯微鏡尺度校正 18
2-4-7 利用原子力顯微鏡觀測高分子薄膜基材微結構 18
2-4-8 利用原子力顯微鏡觀測白蛋白表面微結構 19
2-4-9 檢測溶液 pH 值 19
第三章 結果與討論 20
第一節 基材表面微結構之觀測 20
第二節 不同濃度蛋白質溶液對白蛋白吸附微結構之影響 25
第三節 基材對白蛋白吸附微結構之影響 30
第四節 吸附時間對白蛋白吸附微結構之影響 35
3-4-1 白蛋白吸附於 Mica 隨著時間變化之比較 35
3-4-2 白蛋白吸附於 PMMA 隨著時間變化之比較 39
3-4-3 白蛋白吸附在 PS 隨著時間變化之比較 43
3-4-4 白蛋白吸附於疏水基材上突出顆粒之比較 47
第四章 結論 48
第五章 參考文獻 49


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