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研究生(外文):Novia Nur Rosyida
論文名稱(外文):Studies of Biological Properties of Major Bioactive Compounds from the Stems of Crossostephium chinense (L.) Makino
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蘄艾又稱海芙蓉,生長於台灣與東南亞沿海的石灰岩及珊瑚沉積地區,被當作中草藥使用。這株植物傳統醫學用於治療感冒、麻疹、風濕與支氣管炎。研究指出蘄艾全草萃取物具有抗增生、保肝及抗發炎活性,但是僅有少數莖部具有抗菌、抗氧化與降血壓活性。蘄艾是一種祝明的中草藥,但其在野外並不常見。因此,本研究將對其主要化合物進行分析。有甲醇萃取物中共純化出十個化合物,經由儀器鑑定確定為:lupeol、β-sitostenone、octadecyl (E)-p-coumarate、cycloeucalenol、oleanolic acid、ursolic acid、stigmastan-3-one、betulin、ergone與24-ethyl-5α-cholest-7-en-3β-ol。此外,在生物活性部分則利用抗菌抑菌圈試驗;DPPH抗氧化試驗;ACE抑制試驗;以及MTT及t-BHP細胞試驗。
Abstract (Chinese) i
Abstract (English) ii
Acknowledgement iv
Table of Contents v
List of Tables viii
List of Figures ix

1.1 Background 1
1.2 Research Motivation 2
1.3 Future Impact 2

2.1 Herbal Medicine 3
2.1.1 Crossostephium chinense (L.) Makino 4
2.2 Purification and Identification Methods 6
2.2.1 Maceration 6
2.2.2 Partition 7
2.2.3 Thin Layer Chromatography (TLC) 7
2.2.4 High Performance Liquid Chromatography (HPLC) 9
2.2.5 Nuclear Magnetic Resonance (NMR) 9
2.3 Biological Assays 10
2.3.1 Antimicrobial Assay 10
2.3.2 Antioxidant Assay 13 Antihypertension as an Oxidative Stress Relative Disease 13 DPPH Assay 14 FTC Assay 15 TBA Assay 15
2.3.3 Antihypertensive in vitro Assay 15
2.3.4 Cytotoxic Assay 16
2.3.5 Hepatoprotective Assay 17

3.1 Time and Place 18
3.2 First Research Stage 18
3.2.1 Parts of Plant were Used 19
3.2.2 Sample Preparation 19
3.2.3 Extraction 19
3.2.4 Partition 19
3.2.5 Isolation using Column Chromatography 20
3.2.6 Confirmation by Thin Layer Chromatography (TLC) 22
3.2.7 Separation using High Performance Liquid Chromatography (HPLC) 22
3.2.8 Identification by Nuclear Magentic Resonance (NMR) 22
3.3 Preliminary Assays and Second Research Stage 23
3.3.1 DPPH Assay 23
3.3.2 FTC Assay 23
3.3.3 TBA Assay 24
3.4 Analysis of Data 24
3.5 Research Framework 24
3.6 Biological Assays 25
3.6.1 Antimicrobial Assay 25
3.6.2 Antihypertensive Assay 26
3.6.3 Cytotoxic Assay 26
3.6.4 Hepatoprotective Assay 27

4.1 Preliminary Assays Results 28
4.1.1. DPPH Assay 28
4.1.2. FTC and TBA Assays 28
4.2 First Stage Experiment Result 31
4.2.1 Extraction, Partition, and Isolation 31
4.2.2 1H NMR Results 33
4.3 Second Stage Experimental Result 44
4.3.1 Antimicrobial Activity 45
4.3.2 Antioxidant Activity 45
4.3.3 Antihypertensive Activity 46
4.3.4 Cytotoxic Activity 48
4.3.5 Hepatoprotective Assay 49

5.1 Discussion of Preliminary Assay Results 50
5.2 Discussion of First Stage Experiment Results 51
5.3 Discussion of Second Stage Experiment Result 56
5.3.1 Antimicrobial Activity 56
5.3.2 Antioxidant Activity 58
5.3.3 Antihypertesive Activity 59
5.3.4 Cytotoxic Activity 60
5.3.5 Hepatoprotective Activity 61



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