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研究生:劉倩伶
研究生(外文):LIU, CHIEN-LING
論文名稱:從傳統工藝到綠工藝:論台灣藍染的當代保存與復興
論文名稱(外文):De l’artisanat traditionnel à la technologie verte : la préservation contemporaine et la renaissance de la teinture à l’indigo de Taïwan
指導教授:賴宏亮賴宏亮引用關係
指導教授(外文):Horng-Liang Lay
口試日期:2020-07-20
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:農園生產系所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2020
畢業學年度:108
語文別:法文
論文頁數:165
中文關鍵詞:荖葉DPPH自由基清除能力彈性蛋白酶中性粒細胞
外文關鍵詞:Piper betle L.DPPH free radical scavenging abilityelastaseneutrophils
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荖葉及荖花(Piper betle L.)屬於胡椒科(Piperaceae)、胡椒屬(Piper)為嗜好類特用作物,亦具有消炎、止血及抗肝癌等效用,常被作為香料和止痛藥並用於治療脾胃病。本研究針對荖花地下莖進行成分分離與純化,並將所分離之化合物利用高效液相層析法(High-Performance Liquid Chromato graphy, HPLC)進行荖花不同部位成分之定量分析,並且進行生物活性測試,抗氧化及抗發炎之活性篩選研究。本次試驗管柱層析共分離出6個化合物,其分別依序為Aristololactam A II、Aristololactam B II、Hydroxy chavicol、Piperolactam A、Cepharadione A、2-(’-hydroxychavicol)- hydroxyl chavicol,而其中2-(’-hydroxychavicol)-hydroxychavicol為新化合物。並以Aristololactam A II、Aristololactam B II、Hydroxychavicol、Piperolactam A為指標成分進行探討荖花的地下莖、果序、葉部以及荖葉
含量之變化,結果顯示Hydroxychavicol含量最高,Aristololactam AII於荖花的地下莖和荖花葉部成分含量分別為824.0 ± 22.0、3006.0 ± 41.0 μg/g;另外,荖花果序及荖葉的部分之Piperolactam A其成分為593.0 ± 21.0、6977.0 ± 9.0 μg/g。在DPPH自由基清除能力以乙酸乙酯層可溶物有較好之自由基清除力,其荖花的地下莖IC50值為26.6 μg/mL;另外,酒精萃取物之荖花葉部IC50值為27.2 μg/mL。此外,6個純化合物,進行抗發炎活性試驗,對於嗜中性白血球所引起的超氧自由基生成與彈性蛋白酶釋放有良好的抑制效果,結果顯示,其成分Hydroxychavicol之IC50值為0.74 ±0.26 μg/mL與4.67±0.04 μg/mL,且抑制率分別為104.42±1.52 %與102.01±12.77 %;另外,其次成分為2-(’-hydroxychavicol)-hydroxyl chavicol其IC50值為8.59 ±2.30 μg/mL與13.14±7.05 μg/mL,抑制率分別為94.85±6.14 %與60.24 ±3.82 %。

Piper betle L. , in Piper genus of Piperaceae family, have been used as a traditional medicine. It demonstrated various therapeutic effects including anti-inflammatory, treating for liver cancer, and being used as an anodyne for the stomachache. In this study, the biological activities of rhizome from P. betle were evaluated and the components that brought about the activities were investigated. Six compounds including Aristolo lactam AII, Aristololactam BII, Hydroxychavicol, 2-(’-hydroxychavicol)- hydroxyl chavicol, Piperolactam A, and Cepharadione A were isolated. In which, 2-(’-hydroxylchavicol)-hydroxyl chavicol was a new compound. Contents of Aristololactam AII, Aristo lolactam BII, Hydroxychavicol, and Piperolactam A in different part of P. betle were tested. Hydroxychavicol had the highest content P. betle as compare to other compounds. The content of Aristololactam AII in rhizome from of P. betle and leaf from inflorescence of P. betle were of 824.0 ± 22.0 and 3006.0 ± 41.0 μg/g, respectively. The content of Piperolactam A in inflorescence from and leaf of P. betle were 593.0 ± 21.0 and 6977.0 ± 9.0 μg/g, respectively. The Ethyl acetate extraction of rhizome and ethanol extraction of leaf in P. betle revealed a high free radical (1-Diphenyl-2- Picrylhydrazyl, DPPH) scavenging ability and the IC50 were 26.6 and 27.2 μg/mL, respectively. The results also revealed that Hydroxychavicol and 2-(’-hydroxylchavicol)-hydroxyl chavicol inhibited on superoxide anion generation in FMLP / CB that induced human neutrophils and IC50 were 0.74 ±0.26 and 4.67±0.04 μg/ mL, respectively. Meanwhile, they inhibited on elastase releasing in FMLP / CB that induced human neutrophils and IC50 were 8.59±2.30 and 13.14±7.05 μg/ mL, respectively.
中文摘要....................................................I
英文摘要..................................................III
謝誌.......................................................V
目錄......................................................VI
圖表目錄.................................................VIII
壹、前言....................................................1
貳、前人研究.................................................3
一、植物簡介.................................................3
二、化學成分.................................................6
四、藥理活性.................................................8
參、材料與方法..............................................10
一、材料來源................................................10
二、試驗材料................................................10
(ㄧ)試藥與溶劑..............................................10
(二)層析材料...............................................10
(三)儀器設備...............................................11
三、試驗方法................................................12
(一)荖花的地下莖之化學成分分離與純化............................12
(二)荖花的地下莖、果序、葉部以及荖葉之成分定量分析.................15
(三)荖花的地下莖、果序、葉部以及荖葉之抗氧化活性試驗...............19
(四)純化分離6個化合物之抗發炎活性試驗...........................20
(五)統計分析...............................................20
肆、結果...................................................21
一、荖花的地下莖之成分分離與純化................................21
二、荖花的地下莖、果序、葉部以及荖葉之成分定量分析.................26
(一)HPLC成分分析...........................................26
(二)檢量線製作..............................................26
(三)分析方法之確效試驗.......................................27
(四)荖花的地下莖、果序、葉部以及荖葉之成分定量分析.................28
三、荖花的地下莖、果序、葉部以及荖葉之DPPH自由基清除能力試驗........34
四、純化分離6個化合物之抗發炎活性成分試驗........................38
伍、討論...................................................40
陸、結論...................................................44
參考文獻...................................................45
附錄......................................................50
作者簡介...................................................55

李啟賓(2008)檳榔萃取液對雄鼠內生性抗氧化作用及生殖效應之研究。大仁科技大學環境管理研究所碩士論文。

許育慈(2010)荖葉荖花病蟲害發生與防治。行政院農業委員會台東區改良
場技術專刊。40:1-15。

邱創慶(2012)荖葉扦插繁殖之研究。國立屏東科技大學熱帶農業暨國際合
作系碩士論文。

施惠瀅(2006)檳榔嚼塊組成物影響香菸中NNK代謝之體內研究。國立陽
明大學藥理學研究所碩士論文。

郭玫君、馮惠萍(2005)檳榔、荖花及荖葉抑菌作用之探討。嘉南學報。31:
48-54。

郭聲波(2007)蒟醬(簍葉)的歷史與開發。中國農史。1:1-11。

陳鵬(1996)東南亞的荖葉、檳榔。世界民族。1:66-69。

鄭武燦(2000)台灣植物圖鑑(下冊)。國立編譯館。p. 1038。

賴慧芬(2002)荖葉萃取液對黑色素生合成作用之研究。嘉南藥理科技大學
生物科技研究所碩士論文。

謝文全、林宜信、謝伯舟、張麗晴(2004)台灣常用藥用植物圖鑑第一冊。行政院衛生署中醫藥委員會。p. 105。

Adeltrudes, B. C. and O. O. Marina. 2010. Characterization and evaluation of antimicrobial activity of the essential oil from the leaves of Piper betle L. E-Int. Sci. Res. J. 1(2): 1749-2094.

Ali, I., F. G. Khan, K. A. Suri, B. D. Gupta, N. K. Satti, P. Dutt, F. Afrin, G. N. Qazi, and I. A. Khan. 2010. In vitro antifungal activity of hydroxyl chavicol isolated from Piper betle L. Ann. Clin. Microbiol. Antimicrob. 9: 7.

Anjali, P., B. Sarang, D. Prabhu, and A. S. Krishna. 2010. Modulation of Th1/Th2 cytokines and inflammatory mediators by hydroxychavicol in adjuvant induced arthritic tissues. Cytokine 49: 14-121.

Arambewela, L. S. R., L. D. A. Arawwawala, and W. D. atnasooriya. 2005.
Antidiabetic activities of aqueous and ethanolic extracts of Piper betle leaves in rats. J. Ethnopharmacol. 102: 239-245.

Benesch, L., B. Paul, G. Denis, H. Stephen, X. Wang and S. Victor. 1998. Directed ortho metalation cross coupling connections remote lateral metalation cyclization of 2-1mino-2'-MethyI Biaryls to 9-Amino phenanthrencs. A synthesis of the alkaloid Piperolactam C Tetrahedron Lett. 39: 961-964.

Chang, H. L., F. R. Chang, J. S. Chen, H. P. Wang, Y. H. Wu, C. C. Wang, Y. C. Wu, and T. L. Hwang. 2008. Inhibitory effects of 6-hydroxycleroda- 3,13(14)E-dien-15-oic acid on superoxide anion and elastase release in human neutrophils through multiple mechanisms. Eur. J. Pharmacol. 586: 332-339.

Chen, S. J., B. N. Wu, J. L. Yeh , Y. C. Lo, I. S. Chen , and I. J. Chen 1995. C-fiber-evoked autonomic cardiovascular effects after injection of Piper betle inflorescence extracts. J. Ethnopharmacol. 45: 183-188.

Ghosh, K. and T. K. Bhattacharya. 2005. Chemical constituents of Piper betle Linn. (Piperaceae) roots. Molecules 10: 798-802.

Josip, M., J. Igor, B. Ivica, P. B. Marija, B. Suzana, I. B. Ivana, S. Vilko, Z. Neven, B. K. Krunoslav, V. T. Drazen, and M. Norbert. 2008. Compara- tive study on the antioxidant and biological activities of carvacrol, thymol, and eugenol derivatives. J. Agric. Food. Chem. 56: 3989-3996.

Kumar, N., P. Misra, A. Dube, S. Bhattacharya, M. Dikshit, and S. Ranade. 2010. Piper betle Linn. a maligned Pan-Asiatic plant with an array of pharmacological activities and prospects for drug discovery. Corros. Sci.
99: 7.

Lakshmi, A., M. Arawwawala and D. Rajapaksa. 2006. Piper betle: a potential natural antioxidant. Int. J. Food Sci. Technol. 41: 10-14.

Lin, C. F., Y. L. Leu, S. A. Al-Suwayeh, M. C. Ku, T. L. Hwang, and J. Y. Fang. 2012. Anti-inflammatory activity and percutaneous absorption of quercetin and its polymethoxylated compound and glycosides: the relationships to chemical structures. Eur. J. Pharm. Sci. 47: 857-864.

Lo, W. L., F. R. Chang, and Y. C. Wu. 2000. Alkaloids from the Leaves of Fissistigma glaucescens. J. Chin. Chem. Sic. 7: 1251-1256.

Nalina, T. and Z. H. A. Rahim. 2007. The crude aqueous extract of Piper betle L. and its antibacterial effect towards Streptococcus mutans. Am. J. Biotechnol. Biochem. 3(1): 10-15.
Niranjan, R. Nivedita, R. Ritu, I. and S. Chandrasekaran 2002. Phenolic antibacterials from Piper betle in the prevention of halitosis. J. Ethnopharmacol. 83: 149-152.

Pouységu, L. Sylla, T. Garnier, T. Rojas, L. B. Charris, J. Deffieux, and D. S. Quideau. 2010. Hypervalent iodine-mediated oxygenative phenol dearomatization reactions. Tetrahedron 66: 5908-5917.

Prakash, B. Shukla, R. Singh, P. Kumar, A. Mishra, P. K., and N. K. Dubey. 2010. Efficacy of chemically characterized Piper betle L. essential oil against fungal and aflatoxin contamination of some edible commodities and its antioxidant activity. I. J. F. Food. microbiol. 142: 114-119.

Rao, K. V., and G. C. Reddy. Chemistry of Saururus cernuus, V. 1990. Chem-
stry of Saururus cernuus, V. sauristolactam and other nitrogenous constituents. J. Nat. Prod. 53: 309-312.

Row, L. C. and J. C. Ho. 2009. The antimicrobial activity, mosquito larvicidal activity, antioxidant property and tyrosinase inhibition of Piper betle. J. Chin. Chem. Sic. 56:653-658.

Sarkar, M., P. Gangopadhyay, B. Basak, K. Chakrabarty, J. Banerji, P. Adhi kary, and A. Chatterjee. 2000. The reversible antifertility effect of Piper betle Linn. on Swiss albino male mice. Contraception 62: 271-274.

Sugumaran, M., G. M. Suresh, K. Sankarnarayanan, M. Yokesh, M. Poornima, and R. R. Sree. 2011. Chemical composition and antimicrobial activity of vellaikodi variety of Piper betle Linn Leaf oil against dental pathogens. Inter. J. Pharm. Tech. Res. 3(4): 2135-2139.

Tang, D. W., K. W. Chang, C. W. Chi, and T. Y. Liu 2004. Hydroxy-chavicol modulates benzo[a]pyrene-induced genotoxicity through induction of dihydrodiol dehydrogenase. Toxicol. Lett. 152: 235-243.

Trakranrungsie, N., A. Chatchawanchonteera, and W. Khunkitti. 2008. Eth noveterinary study for antidermatophytic activity of Piper betle, Alpinia galanga and Allium ascalonicum extracts in vitro. Res. Vet. Sci. 84: 80-84.

Xiang, Z., H. Yan, Y. Jian, H. D. Hui, L. L. Yun, D. and B. Lian. 2010. Two new ceramides from the stems of Piper betle L. Chin. Chem. Lett. 21: 433-436.

Virinder, S. P., C. J. Subhash, G. Sangita, A. Sangeet, K. R. Vivek, K. Rajesh, A. Abul, M. Sanjay, K. Naresh, J. Rajni, K. S. Nawal, D. T. Om, J. L. Stephen, E. William, W. H. Oliwer, E. O. Carl, K. S. Sanjay, and W. Jesper. 1998. Polyphenols and alkaloids from piper species. Phytochemistry 38(3): 1069-1078.

Yadav, S. K., B. Adhikary, B. Maity, S. K. Bandyopadhyay, and S. Chattopad hyay. 2009. The gastric ulcer-healing action of allylpyrocatechol is mediated by modulation of arginase metabolism and shift of cytokine balance. Eur. J. Pharmacol. 614: 106-113.

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