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研究生:鄭淑蓉
研究生(外文):Shu-Rung Cheng
論文名稱:青花菜蔬菜幼苗在不同光照時間及不同光質
論文名稱(外文):The Studies of Antioxidant Activity on Different
指導教授:古明萱教授
指導教授(外文):Dr. Ming-Shaiun Guu
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:農園生產系所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:123
中文關鍵詞:十字花科蔬菜抗氧化能力酚類物質花青素黑芥子酶
外文關鍵詞:BrassicaceaeVegetableAntioxidant capacityPhenolic compoundsAnthocyaninsMyrosinase
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青花菜為本土特有的十字花科植物,在臺灣中部地區廣為栽種,它除了熱量低,纖維多,含有豐富的維生素C之外,近年來許多研究顯示,十字花科蔬菜幼苗具有相當良好的抗氧化能力,極具營養價值。
本研究主要目的在探討十字花科蔬菜幼苗的抗氧化能力,透過不同品種、不同光照及不同光質進行試驗。試驗分成三個部分:(一) 不同生長日數十字花科蔬菜幼苗之抗氧化能力。(二) 不同品種青花菜幼苗在不同光照時間之抗氧化能力。(三) 不同光質及光照時間十字花科蔬菜幼苗之抗氧化能力。
從不同品種及不同生長日數十字花科蔬菜幼苗的抗氧化能力試驗結果發現,十字花科蔬菜幼苗的總酚含量、清除DPPH自由基能力、清除超氧陰離子能力及總還原能力都不因生長日數不同而有所差異。而螫合亞鐵離子能力、總抗氧化能力都在生長日數14天時達到最高。至於類黃酮含量由高至低分別是綠王、青江菜、永翠及油菜;花青素含量則以綠王及油菜較高;維生素C含量以綠王及青江菜較高;螫合亞鐵離子能力、總還原能力及總抗氧化能力,由高至低分別是綠王、青江菜、油菜及永翠。綜上,青花菜中的綠王及十字花科青江菜明顯呈現較高的抗氧化能力。
不同品種青花菜幼苗(綠王及永翠)在不同光照時間的抗氧化能力試驗發現,綠王的總酚含量明顯比永翠高;綠王與永翠都在光照18hr展現最高的總酚含量、維生素C含量、清除DPPH自由基能力、螫合亞鐵離子能力及總還原能力。綠王與永翠在不同光照時間之類黃酮含量、花青素含量、維生素C含量、清除DPPH自由基能力、螫合亞鐵離子能力、清除超氧陰離子能力、總還原能力及總抗氧化能力均無顯著差異。綜上,除綠王的總酚含量明顯比永翠高之外,不同光照時間綠王與永翠的抗氧化能力沒有顯著差異,而且都在光照18hr展現較高的抗氧化能力。
不同光照時間十字花科蔬菜幼苗總酚含量、類黃酮含量、維生素C含量、清除DPPH自由基能力、總還原能力、總抗氧化能力、苯丙氨酸解酶活性及黑芥子酶活性皆有顯著差異,試驗數值也都呈現在光照18hr較高的現象,亦即隨著光照時間越長十字花科蔬菜幼苗的抗氧化能力越高。從不同光質十字花科蔬菜幼苗的抗氧化能力試驗發現,在白光下成長的十字花科蔬菜幼苗類黃酮含量明顯較高;在紅光下成長的十字花科蔬菜幼苗總抗氧化能力及黑芥子酶活性明顯較高;黃光下成長的十字花科蔬菜幼苗苯丙氨酸解酶活性及黑芥子酶活性明顯較高。不同光質及光照時間十字花科蔬菜幼苗之抗氧化能力試驗發現,綠王的總酚含量明顯較高;油菜的維生素C含量及清除DPPH自由基能力明顯較高;青江菜的螫合亞鐵離子能力、總抗氧化能力、苯丙氨酸解酶活性明顯較高。
總之,青花菜中的綠王及十字花科青江菜具有較高的抗氧化能力;光照時間越長十字花科蔬菜幼苗的抗氧化能力越高;不同光質對十字花科蔬菜幼苗的抗氧化能力有不同的影響。整體觀察,青江菜價格較低,又展現頗佳的抗氧化能力,值得推介。

Brassica oleracea is a Cruciferous herb unique to native land and is widely planted in the central part of Taiwan. In addition to low calories, more fiber and rich vitamin C, its anti-cancer effect is highly commendable. In recent years, many studies have shown that Cruciferous vegetable seedlings have quite a good antioxidant activity and great nutritional value.
This study aims to explore the antioxidant activity on Cruciferous vegetable seedlings by conducting tests on different kinds, different lights and different light qualities. Tests are divided into three parts: (a) the antioxidant activity on Cruciferous vegetable seedlings under different growth days; (b) the antioxidant activity on different kinds of B. oleracea seedlings under different hours of light; (c) the antioxidant activity on Cruciferous vegetable seedling under different light qualities and hours of light.
It’s found from the test results of the antioxidant activity on Cruciferous vegetable seedlings under different kinds and different growth days that Cruciferous vegetable seedlings do not vary in total phenolic content, DPPH radical scavenging capacity, superoxide anion scavenging capacity and total reducing capacity due to different growth days. The chelate achieves the highest antioxidant activity on the 14th growth day. The vegetables with the flavonoids content from high to low are Green King Broccoli, B. campestris L., Wing Chui Broccoli and B. campestris L. var. amplexicaulis Makino; the anthocyanin content of Green King Broccoli and B. campestris L. var. amplexicaulis Makino is higher; the vitamin C content of Green King Broccoli and B. campestris L. is higher; ferrous ion chelating capacity, total reducing capacity and total antioxidant activity from high to low are Green King Broccoli, B. campestris L., B. campestris L. var. amplexicaulis Makino and Wing Chui Broccoli. In summary, Green King Broccoli and B. campestris L. obviously show higher antioxidant activity.
It’s found from the test results of the antioxidant activity on different kinds of B. oleracea seedlings (Green King Broccoli and Wing Chui Broccoli) under different hours of light that the total phenolic content of Green King Broccoli is significantly higher than that of Wing Chui Broccoli; both Green King Broccoli and Wing Chui Broccoli show the highest total phenolic content, vitamin C content, DPPH radical scavenging capacity, ferrous ion chelating capacity and total reducing capacity with 18 hours of light. The flavonoids content, the anthocyanin content, vitamin C content, DPPH radical scavenging capacity, ferrous ion chelating capacity, superoxide anion scavenging capacity, total reducing capacity and total antioxidant activity of Green King Broccoli and Wing Chui Broccoli under different hours of light have no significant differences. In summary, besides that the total phenolic content of Green King Broccoli is significantly higher than that of Wing Chui Broccoli, Green King Broccoli and Wing Chui Broccoli under different hours of light have no significant differences in antioxidant activity and show higher antioxidant activity with 18 hr of light.
The total phenolic content, the flavonoids content, vitamin C content, DPPH radical scavenging capacity, total reducing capacity, total antioxidant activity, Phenyl alanineammonialyase activity and myrosinase activity of Cruciferous vegetable seedlings under different hours of light have significant differences; the test value also shows higher with 18 hours of light, i.e. the antioxidant activity on Cruciferous vegetable seedlings becomes higher with longer hours of light. It’s found from the test results of the antioxidant activity on Cruciferous vegetable seedlings under different light qualities that Cruciferous vegetable seedlings growing up in white light have significantly higher flavonoid content; Cruciferous vegetable seedlings growing up in yellow light have significantly higher Phenyl alanineammonialyase activity and myrosinase activity. It’s found from the test results of the antioxidant activity on Cruciferous vegetable seedlings under different light qualities and hours of light that the total phenolic content of Green King Broccoli is significantly higher; the vitamin C content and DPPH radical scavenging capacity of B. campestris L. var. amplexicaulis Makino are significantly higher; ferrous ion chelating capacity, total reducing capacity and Phenyl alanineammonialyase activity of B. campestris L. are significantly higher.
In short, Green King Broccoli and B. campestris L. have higher antioxidant activity; Cruciferous vegetable seedlings with longer hours of light have higher antioxidant activity; different light qualities have different effects on the antioxidant activity on Cruciferous vegetable seedlings. Overall, the price of B. campestris L. is lower, but it has quite good antioxidant activity, worth recommending.

中文摘要………………………………………………………………...Ⅰ
英文摘要………………………………………………………………...Ⅲ
目錄…………………………………………………………………..…VI
圖表目錄………………………………………………………….….....VII

壹、前言………………………………………………………………....... 1
貳、前人研究…………………………………………………………....... 4
一、試驗用十字花科蔬菜之介紹………………………………...... 4
二、自由基與活性氧.………………………………………….......... 5
三、自由基與抗氧化防禦系統……………………………………... 7
四、天然抗氧化物作用機制…………………………………......…. 8
五、影響植物抗氧化能力之因素……………………………....... 14
參、材料與方法……………………………………………………….. .22
一、不同生長日數青花菜幼苗之抗氧化能力………………....… 22
二、不同品種青花菜幼苗在不同光照時間之抗氧化能力…......... 27
三、不同光質及光照時間對青花菜蔬菜幼苗抗氧化能力影響…. 30
肆、結果…………………………………………….……………….......35
一、不同生長日數十字花科蔬菜幼苗之抗氧化能力…………......35
二、不同品種青花菜幼苗在不同光照時間之抗氧化能力……......50
三、不同光質及光照時間十字花科蔬菜幼苗之抗氧化能力……..62
伍、討論 …………………………………………………………….......88
一、不同生長日數十字花科蔬菜幼苗之抗氧化能力...……….....88
二、不同品種青花菜幼苗在不同光照時間之抗氧化能力..…........93
三、不同光質及光照時間十字花科蔬菜幼苗之抗氧化能力....….95
陸、結論….…………………………………………………………...…103
參考文獻…………………………………………………………….….104

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