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研究生(外文):Hsien-Pin Hu
論文名稱(外文):Using dihydroxynaphthalene melanin biosynthesis genes in developing biochip for identification and phylogenetic study of Colletotrichum
外文關鍵詞:ColletotrichumDHN-melanin biosynthesis geneSytalone dehydratase138-trihydroxynaphthalene reductaseoligocleotide microarray.
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傳統上炭疽病菌屬(Colletotrichum)之鑑定主要依據植物寄主範圍及型態構造特徵,但常因重疊導致分類混淆和鑑定困難,甚至利用單一基因當為分子標記,用以診斷鑑定不同種的炭疽病菌,但因序列彼此相似度太高、解序力低而無法區別並釐清親緣關係。目前多基因座分析為探討炭疽病菌類緣及種間鑑定之主要方法,但需耗費較多人力、物力與時間。為克服此種障礙,遂進行探討與致病性相關之黑色素生合成基因,在炭疽病菌診斷鑑定及類緣關係應用之可能性。研究結果顯示炭疽病菌屬之黑色素生合成基因Scytalone dehydratase(SCD)在種間相似度為70~100%、1,3,8-trihydroxynaphthalene reductase(THR)則為78~100%,相似度不高有利於類緣分析及種間鑑定。此外,兩基因皆具有較長片段的內插子,也有利於設計寡核苷酸探針。分別應用SCD(651 bp)及THR(1,286 bp)基因片段,以RaxML之最大似然性分析,建構59種代表性炭疽病菌,皆可將其區分為9個支序群(clade),且類緣樹末端種間分支少有多支性,顯示此二基因皆可用以區辨不同種之炭疽病菌。利用SCD進一步探討感染草莓、紅棗之51株炭疽病菌分離株,47株屬於gloeosporioides clade,4株則屬boninense clade,其病原皆非以往所記載之C. gloeosporioides、C. acutatum、C. theobromicola (syn. C. fragariae),其中除C. asianum/C. fructicola、C. boninense外,位在gloeosporioides clade中出現頻率89%之菌株可能為一新種。另外ML443、ML463、ML348對草莓之病原性也經柯霍氏法則(Koch''s postulates)證明。此分離株可以感染草莓,並造成草莓冠部褐化、全株萎凋。此外,利用黑色素生合成基因SCD,所完成設計之寡核苷酸生物晶片,可鑑別28種涵蓋國內外嚴重危害具有經濟價值農園藝作物之重要炭疽病菌。也應用SCD基因當為炭疽病菌分子標記,試圖解決由食品工業研究所之生物資源保存及研究中心所保存之26株炭疽病菌身分及分類問題,並提出應修訂的菌株及可能為特有種之名單。未來將進一步蒐集文獻序列,分析更多炭疽病菌菌株,應用黑色素生合成基因或多基因座分析釐清此26株炭疽病菌株之屬性。本研究結果顯示黑色素生合成基因可資應用於炭疽病菌屬之類緣分析,而所完成設計之生物晶片,未來將有助於炭疽病菌之防檢疫、生態學、病害防治管理及流行病學等研究。

Traditionally, identification of the Colletotrichum is mainly based on the morphological characters and host specificity, but often confused due to the overlap of identification parameters. The same status also occurs to the ITS molecular markers, owing to the high similarity of gene sequence, resulting in the low resolution in taxa discrimination and phylogenetic analysis. Even though more recently the use of multilocus genes as a barcode for the study of Colletotrichum are widely accepted, however, their usage are limited by its high expenditure of resources. To circumvent these obstacles, attempt using the pathogenesis correlated dihydroxynaphthalene (DHN)-melanin in clarification of the taxonomy and phylogeny of Colletotrichum were initiated. The outcome revealed the similarity of DHN-melanin biosynthesis genes scytalone dehydratase (SCD) and 1,3,8-trihydroxynaphthalene reductase (THR) among Colletotrichum range from 70-100% and 78-100%, respectively. The low sequence similarity will facilitate taxonomic and phylogenetic study of Colletotrichum. In other aspects, the long length of introns of these genes also favor the relvant study. By using the SCD (651 bp) and THR (1,286 bp), the 59 representative Colletotrichum species can be unambiguously divided into nine clades without terminal polytomy in constructed tree inferred by the RaxML method. The results implicated the great potential of SCD and THR genes in fabricating biochip. Furthermore, investigation of the 51 Colletotrichum strains isolated from infested strawberry and red jujube seedlings using SCD as barcode, which clearly indicated the causal organisms were not C. gloeosporioides, C. acutatum, C. theobromicola (syn. C. fragariae), instead, except C. asianum/C. fructicola and C. boninense, one of the endemic strain located in gloeosporioides clade with 92% incidence might be the causal pathogen. Besides, the pathogenicity of the Colletotrichum ML348, ML443, ML463 towards strawberry was demonstrated via Koch’s postulates. These strains could infect strawberry and led to the root crown browning and seedling wilting. Additionally, by using DHN-melanin SCD gene, a biochip developed could simultaneously, rapidly and precisely identified 28 crucial Colletotrichum species, which could devastate the crucial agronomic and horticultural crops worldwide. Likewise, by SCD bacode, 26 endemic Colletotrichum species deposited in Bioresource Collection and Research Centre, FIRDI, their identity were clarified. To justify our sanction, additional references, sequence, and Colletotrichum holotype or epitype species will be collected, sequenced and analysed. In conclusion, the DHN- melanin biosynthesis genes can be used in phylogenetic study of Colletotrichum, and the biochip developed can be used in quarantine, ecology, disease control and management, and epidemiology study.

口試委員會審定書 #
誌謝 i
中文摘要 ii
Chapter 1 前人研究 10
1.1 真菌之分子標記 10
1.2 炭疽病菌(Colletotrichum spp.) 11
1.3 炭疽病菌之分子標記 14
1.4 炭疽病菌分類演變 15
1.5 黑色素為病原因子 16
1.6 草莓炭疽病 18
1.7 傳統鑑定診斷植物病原真菌 19
1.8 分子生物學檢測病原真菌 20
Chapter 2 材料與方法 25
2.1 菌株與培養 25
2.2 菌體核苷酸(DNA)之萃取 25
2.3 目標片段之增幅(PCR反應) 26
2.4 PCR產物電泳分析 26
2.5 PCR產物純化 27
2.6 基因片段之選殖、定序 27
2.7 探針設計 29
2.8 晶片製備 29
2.9 雜合反應 30
2.10 草莓炭疽病菌接種實驗 32
Chapter 3 結果 33
3.1 多基因座類緣分析 33
3.2 黑色素生合成相關基因選殖與分析 34
3.3 SCD類緣樹 35
3.4 THR類緣樹 36
3.5 草莓炭疽病族群分析 37
3.6 草莓炭疽病菌病原性柯霍式法則驗證 38
3.7 炭疽病菌屬晶片之研發 38
3.8 台灣本土炭疽病菌分析 39
Chapter 4 討論 42
Chapter 5 References 52
Chapter 6 Tables 60
Chapter 7 Figures 76
Chapter 8 附錄 101

中華民國植物病理學會。2002。台灣植物病害名彙 第四版。台灣
Adamczyk, J., M. Hesselsoe, N. Iversen, M. Horn, A. Lehner, P. H. Nielsen, M. Schloter, P. Roslev and M. Wagner. 2003. The isotope array, a new tool that employs substrate-mediated labeling of rRNA for determination of microbial community structure and function. Applied and Environmental Microbiology 69: 6875-6887.
Adaskaveg, J. and R. Hartin. 1997. Characterization of Colletotrichum acutatum isolates causing anthracnose of almond and peach in California. Phytopathology 87: 979-987.
Álvarez, E., A. Arenas and J. F. Mejía. 2006. Molecular and pathogenic characterization of isolates of Colletotrichum spp. associated with anthracnose of andean blackberry on accessions from Valle del Cauca. Annual Report 2006 Project IP-06: 17.
Asakura, M., K. Yoshino, A. M. Hill, Y. Kubo, Y. Sakai and Y. Takano. 2012. Primary and secondary metabolism regulates lipolysis in appressoria of Colletotrichum orbiculare. Fungal Genetics and Biology 49: 967-975.
Begerow, D., H. Nilsson, M. Unterseher and W. Maier. 2010. Current state and perspectives of fungal DNA barcoding and rapid identification procedures. Applied Microbiology and Biotechnology 87: 99-108.
Begerow, D., M. Stoll and R. Bauer. 2006. A phylogenetic hypothesis of Ustilaginomycotina based on multiple gene analyses and morphological data. Mycologia 98: 906-916.
Bodrossy, L. and A. Sessitsch. 2004. Oligonucleotide microarrays in microbial diagnostics. Current Opinion in Microbiology 7: 245-254.
Cai, L., K. Hyde, P. Taylor, B. Weir, J. Waller, M. Abang, J. Zhang, Y. Yang, S. Phoulivong and Z. Liu. 2009. A polyphasic approach for studying Colletotrichum. Fungal Diversity 39: 183-204.
Campa, D., A. Tavanti, F. Gemignani, C. S. Mogavero, I. Bellini, F. Bottari, R. Barale, S. Landi and S. Senesi. 2008. DNA microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses. Journal of Clinical Microbiology 46: 909-915.
Cannon, P. F., A. G. Buddie and P. D. Bridge. 2008. The typification of Colletotrichum gloeosporioides. Mycotaxon 104: 189-204.
Cannon, P. F., U. Damm, P. Johnston and B. Weir. 2012. Colletotrichum–current status and future directions. Studies in Mycology 73: 181-213.
Chang, C.-H., T.-S. Lin and W.-J. Yang. 2012. Invading time of Colletotrichum gloeosporioides affects fruit drop and infection rate in ‘Irwin’mango (Mangifera indica L.). Horticulture, Environment, and Biotechnology 53: 452-459.
Chen, W., Z. R. Djama, M. D. Coffey, F. N. Martin, G. J. Bilodeau, L. Radmer, G. Denton and C. A. Lévesque. 2013. Membrane-based oligonucleotide array developed from multiple markers for the detection of many Phytophthora species. Phytopathology 103: 43-54.
Chen, W., K. Seifert and C. Lévesque. 2009. A high density COX1 barcode oligonucleotide array for identification and detection of species of Penicillium subgenus Penicillium. Molecular Ecology Resources 9: 114-129.
Chen, Y., R. Conner, C. Gillard, D. McLaren, G. Boland, P. Balasubramanian, C. Stasolla, Q. Zhou, S. Hwang and K. Chang. 2012. A quantitative real‐time PCR assay for detection of Colletotrichum lindemuthianum in navy bean seeds. Plant Pathology 62: 900-907.
Cheney, S. A., N. J. Lafranchi‐Trisrem, D. Bourges and E. U. Canning. 2001. Relationships of microsporidian genera, with emphasis on the polysporous genera, revealed by sequences of the largest subunit of RNA polymerase II (RPB1). Journal of Eukaryotic Microbiology 48: 111-117.
Clewley, J. P. 2004. A role for arrays in clinical virology: fact or fiction? Journal of Clinical Virology 29: 2-12.
Crouch, J. A. and L. A. Beirn. 2009. Anthracnose of cereals and grasses. Fungal Diversity 39: 19-44.
Crouch, J. A., B. B. Clarke and B. I. Hillman. 2009. What is the value of ITS sequence data in Colletotrichum systematics and species diagnosis? A case study using the falcate-spored graminicolous Colletotrichum group. Mycologia 101: 648-656.
Crouch, J. A., B. B. Clarke, J. F. White Jr and B. I. Hillman. 2009. Systematic analysis of the falcate-spored graminicolous Colletotrichum and a description of six new species from warm-season grasses. Mycologia 101: 717-732.
Crouch, J. A., L. P. Tredway, B. B. Clarke and B. I. Hillman. 2009. Phylogenetic and population genetic divergence correspond with habitat for the pathogen Colletotrichum cereale and allied taxa across diverse grass communities. Molecular Ecology 18: 123-135.
Damm, U., R. Baroncelli, L. Cai, Y. Kubo, R. O''Connell, B. Weir, K. Yoshino and P. F. Cannon. 2010. Colletotrichum: species, ecology and interactions. IMA Fungus 1: 161-165.
Damm, U., P. Cannon, J. Woudenberg and P. Crous. 2012. The Colletotrichum acutatum species complex. Studies in Mycology 73: 37-113.
Damm, U., P. Cannon, J. Woudenberg, P. Johnston, B. Weir, Y. Tan, R. Shivas and P. Crous. 2012. The Colletotrichum boninense species complex. Studies in Mycology 73: 1-36.
Damm, U., J. Woudenberg, P. Cannon and P. Crous. 2009. Colletotrichum species with curved conidia from herbaceous hosts. Fungal Diversity 39: 45-87.
Dean, R., J. A. L. Van Kan, Z. A. Pretorius, K. E. Hammond‐Kosack, A. Di Pietro, P. D. Spanu, J. J. Rudd, M. Dickman, R. Kahmann and J. Ellis. 2012. The Top 10 fungal pathogens in molecular plant pathology. Molecular Plant Pathology 13: 414-430.
Doyle, V. P., P. V. Oudemans, S. A. Rehner and A. Litt. 2013. Habitat and host indicate lineage identity in Colletotrichum gloeosporioides sl from wild and agricultural landscapes in north America. PLoS ONE 8: e62394.
Duke, S. O., M. Gohbara, R. N. Paul and M. V. Duke. 1992. Colletotrichin causes rapid membrane damage to plant cells. Journal of Phytopathology 134: 289-305.
El Gueddari, N. E., U. Rauchhaus, B. M. Moerschbacher and H. B. Deising. 2002. Developmentally regulated conversion of surface‐exposed chitin to chitosan in cell walls of plant pathogenic fungi. New Phytologist 156: 103-112.
Freeman, S. and T. Katan. 1997. Identification of Colletotrichum species responsible for anthracnose and root necrosis of strawberry in Israel. Phytopathology 87: 516-521.
Frisvad, J. C. and R. A. Samson. 2004. Polyphasic taxonomy of Penicillium subgenus Penicillium. A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins. Studies in Mycology 49: C174.
Fujihara, N., A. Sakaguchi, S. Tanaka, S. Fujii, G. Tsuji, T. Shiraishi, R. O''Connell and Y. Kubo. 2010. Peroxisome biogenesis factor PEX13 is required for appressorium-mediated plant infection by the anthracnose fungus Colletotrichum orbiculare. Molecular Plant-Microbe Interactions 23: 436-445.
Gan, P., K. Ikeda, H. Irieda, M. Narusaka, R. J. O''Connell, Y. Narusaka, Y. Takano, Y. Kubo and K. Shirasu. 2013. Comparative genomic and transcriptomic analyses reveal the hemibiotrophic stage shift of Colletotrichum fungi. New Phytologist 197: 1236-1249.
Garnica, S., M. Weiß, B. Oertel, J. Ammirati and F. Oberwinkler. 2009. Phylogenetic relationships in Cortinarius, section Calochroi, inferred from nuclear DNA sequences. BMC Evolutionary Biology 9: 1-17.
Geiser, D., M. Klich, J. C. Frisvad, S. Peterson, J. Varga and R. A. Samson. 2007. The current status of species recognition and identification in Aspergillus. Studies in Mycology 59: 1-10.
Gilmore, S. R., T. Graefenhan, G. Louis‐Seize and K. A. Seifert. 2009. Multiple copies of cytochrome oxidase 1 in species of the fungal genus Fusarium. Molecular Ecology Resources 9: 90-98.
Guerber, J. C., B. Liu, J. C. Correll and P. R. Johnston. 2003. Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility. Mycologia 95: 872-895.
Hebert, P. D., A. Cywinska and S. L. Ball. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences 270: 313-321.
Howard, C. M., J. Maas, C. Chandler and E. Albregts. 1992. Anthracnose of strawberry caused by the Colletotrichum complex in Florida. Plant Disease 76: 976-981.
Hsiao, C. R., L. Huang, J.-P. Bouchara, R. Barton, H. C. Li and T. C. Chang. 2005. Identification of medically important molds by an oligonucleotide array. Journal of Clinical Microbiology 43: 3760-3768.
Hsu, Y. C., T. J. Yeh and Y. C. Chang. 2005. A new combination of RT-PCR and reverse dot blot hybridization for rapid detection and identification of potyviruses. Journal of Virological Methods 128: 54-60.
Huang, F., G. Chen, X. Hou, Y. Fu, L. Cai, K. Hyde and H. Li. 2013. Colletotrichum species associated with cultivated citrus in China. Fungal Diversity: 1-14.
Hyde, K., L. Cai, P. Cannon, J. Crouch, P. Crous, U. Damm, P. Goodwin, H. Chen, P. Johnston and E. Jones. 2009. Colletotrichum names in current use. Fungal Diversity 39: 147-182.
Hyde, K. D. and Y. Zhang. 2008. Epitypification: should we epitypify? Journal of Zhejiang University-Science B 9: 842-846.
Kubo, Y. 2012. Appressorium function in Colletotrichum orbiculare and prospect for genome based analysis. In Morphogenesis and Pathogenicity in Fungi, Topics in Current Genetics (J. Pe′rez-Martı′n and A. D. Pietro, eds) p.115-131 Berlin, Heidelberg : Springer
Kubo, Y., I. Furusawa and M. Yamamoto. 1984. Regulation of melanin biosynthesis during appressorium formation in Colletotrichum lagenarium. Experimental Mycology 8: 364-369.
Kubo, Y., K. Suzuki, I. Furusawa and M. Yamamoto. 1985. Melanin biosynthesis as a prerequisite for penetration by appressoria of Colletotrichum lagenarium: Site of inhibition by melanin-inhibiting fungicides and their action on appressoria. Pesticide Biochemistry and Physiology 23: 47-55.
Kubo, Y. and Y. Takano. 2013. Dynamics of infection-related morphogenesis and pathogenesis in Colletotrichum orbiculare. Journal of General Plant Pathology: 1-10.
Kubo, Y., Y. Takano, N. Endo, N. Yasuda, S. Tajima and I. Furusawa. 1996. Cloning and structural analysis of the melanin biosynthesis gene SCD1 encoding scytalone dehydratase in Colletotrichum lagenarium. Applied and Environmental Microbiology 62: 4340-4344.
Letowski, J., R. Brousseau and L. Masson. 2003. DNA microarray applications in environmental microbiology. Analytical Letters 36: 3165-3184.
Librado, P. and J. Rozas. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 1451-1452.
Lievens, B., M. Brouwer, A. C. Vanachter, C. A. Lévesque, B. Cammue and B. P. Thomma. 2003. Design and development of a DNA array for rapid detection and identification of multiple tomato vascular wilt pathogens. FEMS Microbiology Letters 223: 113-122.
Lievens, B., M. Brouwer, A. C. Vanachter, C. A. Lévesque, B. Cammue and B. P. Thomma. 2005. Quantitative assessment of phytopathogenic fungi in various substrates using a DNA macroarray. Environmental Microbiology 7: 1698-1710.
Lijuan, P., Y. Youlian, D. H. Kevin, A. H. Bahkali and L. Zuoyi. 2012. Colletotrichum species on Citrus leaves in Guizhou and Yunnan provinces, China. Cryptogamie, Mycologie 33: 267-283.
Lin, S. Y., S. Okuda, K. Ikeda, T. Okuno and Y. Takano. 2012. LAC2 encoding a secreted laccase is involved in appressorial melanization and conidial pigmentation in Colletotrichum orbiculare. Molecular Plant-Microbe Interactions 25: 1552-1561.
Liu, B., F. J. Louws, T. B. Sutton and J. C. Correll. 2012. A rapid qualitative molecular method for the identification of Colletotrichum acutatum and C. gloeosporioides. European Journal of Plant Pathology 132: 1-15.
MacKenzie, S., J. Mertely, T. Seijo and N. Peres. 2008. Colletotrichum fragariae is a pathogen on hosts other than strawberry. Plant Disease 92: 1432-1438.
MacKenzie, S., N. A. Peres, M. Barquero, L. Arauz and L. Timmer. 2009. Host range and genetic relatedness of Colletotrichum acutatum isolates from fruit crops and leatherleaf fern in Florida. Phytopathology 99: 620-631.
MacKenzie, S., T. Seijo, D. Legard, L. Timmer and N. Peres. 2007. Selection for pathogenicity to strawberry in populations of Colletotrichum gloeosporioides from native plants. Phytopathology 97: 1130-1140.
Maharaj, A. and S. N. Rampersad. 2012. Genetic differentiation of Colletotrichum gloeosporioides and C. truncatum associated with anthracnose disease of papaya (Carica papaya L.) and bell pepper (Capsium annuum L.) based on ITS PCR-RFLP fingerprinting. Molecular Biotechnology 50: 1-13.
Marshall, A. and J. Hodgson. 1998. DNA chips: an array of possibilities. Nature Biotechnology 16: 27-32.
Matheny, P. B., Y. J. Liu, J. F. Ammirati and B. D. Hall. 2002. Using RPB1 sequences to improve phylogenetic inference among mushrooms (Inocybe, Agaricales). American Journal of Botany 89: 688-698.
Miller, M. A., W. Pfeiffer and T. Schwartz. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In Proceedings of the Gateway Computing Environments Workshop (GCE) p.1-8 New Orleans. New York, USA, Institute of Electrical and Electronics Engineers (IEEE).
Mills, P. R., S. Sreenivasaprasad and A. E. Brown. 1992. Detection and differentiation of Colletotrichum gloeosporioides isolates using PCR. FEMS Microbiology letters 98: 137-143.
Moriwaki, J., T. Sato and T. Tsukiboshi. 2003. Morphological and molecular characterization of Colletotrichum boninense sp. nov. from Japan. Mycoscience 44: 47-53.
Moriwaki, J., T. Tsukiboshi and S. Toyozo. 2002. Grouping of Colletotrichum species in Japan based on rDNA sequences. Journal of General Plant Pathology 68: 307-320.
Munda, A., S. Radisek, J. u tar-Vozlic and B. Javornik. 2011. Genetic variability of Colletotrichum lindemuthianum isolates from Slovenia and resistance of local Phaseolus vulgaris germplasm. Journal for Plant Diseases and Plant Protection 1: 23-29.
Nakaune, R. and M. Nakano. 2007. Benomyl resistance of Colletotrichum acutatum is caused by enhanced expression of β-tubulin 1 gene regulated by putative leucine zipper protein CaBEN1. Fungal Genetics and Biology 44: 1324-1335.
Nithya, K., K. A. I. M. Bukhari, V. Valluvaparidasan, V. Paranidharan and R. Velazhahan. 2012. Molecular detection of Colletotrichum falcatum causing red rot disease of sugarcane (Saccharum officinarum) using a SCAR marker. Annals of Applied Biology 160: 168-173.
O''Connell, R. J., M. R. Thon, S. Hacquard, S. G. Amyotte, J. Kleemann, M. F. Torres, U. Damm, E. A. Buiate, L. Epstein and N. Alkan. 2012. Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses. Nature Genetics 44: 1060-1065.
O''Donnell, K., D. A. Sutton, M. G. Rinaldi, B. A. Sarver, S. A. Balajee, H.-J. Schroers, R. C. Summerbell, V. A. Robert, P. W. Crous and N. Zhang. 2010. Internet-accessible DNA sequence database for identifying fusaria from human and animal infections. Journal of Clinical Microbiology 48: 3708-3718.
O''Neill, N. R., P. van Berkum, J. J. Lin, J. Kuo, G. N. Ude, W. Kenworthy and J. A. Saunders. 1997. Application of amplified restriction fragment length polymorphism for genetic characterization of Colletotrichum pathogens of alfalfa. Phytopathology 87: 745-750.
O’Connell, R. J. and R. Panstruga. 2006. Tête à tête inside a plant cell: establishing compatibility between plants and biotrophic fungi and oomycetes. New Phytologist 171: 699-718.
Padder, B., P. Sharma, O. Sharma and V. Kapoor. 2007. Genetic diversity and gene flow estimates among five populations of Colletotrichum lindemuthianum across Himachal Pradesh. Physiological and Molecular Plant Pathology 70: 8-12.
Pain, N., R. O''connell, J. Bailey and J. Green. 1992. Monoclonal antibodies which show restricted binding to four Colletotrichum species: C. lindemuthianum, C. malvarum, C. orbiculare and C. trifolii. Physiological and Molecular Plant Pathology 41: 111-126.
Peng, L.-J., T. Sun, Y.-L. Yang, L. Cai, K. D. Hyde, A. H. Bahkali and Z.-Y. Liu. 2013. Colletotrichum species on grape in Guizhou and Yunnan provinces, China. Mycoscience 54: 29-41.
Perfect, S. E., H. B. Hughes, R. J. O''Connell and J. R. Green. 1999. Colletotrichum: A model genus for studies on pathology and fungal–plant Interactions. Fungal Genetics and Biology 27: 186-198.
Perpetua, N., Y. Kubo, N. Yasuda, Y. Takano and I. Furusawa. 1996. Cloning and characterization of a melanin biosynthetic THR1 reductase gene essential for appressorial penetration of Colletotrichum lagenarium. Molecular Plant-Microbe Interactions 9: 323-329.
Prihastuti, H., L. Cai, H. Chen, E. McKenzie and K. Hyde. 2009. Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Diversity 39: 89-109.
Prom, L., R. Perumal, S. Erattaimuthu, C. Little, E. No, J. Erpelding, W. Rooney, G. Odvody and C. Magill. 2012. Genetic diversity and pathotype determination of Colletotrichum sublineolum isolates causing anthracnose in sorghum. European Journal of Plant Pathology 133: 671-685.
Rakotoniriana, E. F., J. Scauflaire, C. Rabemanantsoa, S. Urveg-Ratsimamanga, A.-M. Corbisier, J. Quetin-Leclercq, S. Declerck and F. Munaut. 2013. Colletotrichum gigasporum sp. nov., a new species of Colletotrichum producing long straight conidia. Mycological Progress 12: 403-412.
Saiki, R. K., S. Scharf, F. Faloona, K. B. Mullis, G. T. Horn, H. A. Erlich and N. Arnheim. 1985. Enzymatic amplification of b-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230: 1350-1354.
Samuelian, S. K., L. A. Greer, S. Savocchia and C. C. Steel. 2011. Detection and monitoring of Greeneria uvicola and Colletotrichum acutatum development on grapevines by real-time PCR. Plant Disease 95: 298-303.
Schoch, C. L., K. A. Seifert, S. Huhndorf, V. Robert, J. L. Spouge, C. A. Levesque, W. Chen, E. Bolchacova, K. Voigt and P. W. Crous. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences 109: 6241-6246.
Schoch, C. L., G.-H. Sung, F. López-Giráldez, J. P. Townsend, J. Miadlikowska, V. Hofstetter, B. Robbertse, P. B. Matheny, F. Kauff and Z. Wang. 2009. The Ascomycota tree of life: a phylum-wide phylogeny clarifies the origin and evolution of fundamental reproductive and ecological traits. Systematic Biology 58: 224-239.
Seifert, K. A., R. A. Samson, J. R. DeWaard, J. Houbraken, C. A. Lévesque, J. M. Moncalvo, G. Louis-Seize and P. D. N. Hebert. 2007. Prospects for fungus identification using CO1 DNA barcodes, with Penicillium as a test case. Proceedings of the National Academy of Sciences 104: 3901-3906.
Shen, S., P. Goodwin and T. Hsiang. 2001. Infection of Nicotiana species by the anthracnose fungus, Colletotrichum orbiculare. European Journal of Plant Pathology 107: 767-773.
Sherriff, C., M. J. Whelan, G. M. Arnold, J.-F. Lafay, Y. Brygoo and J. A. Bailey. 1994. Ribosomal DNA sequence analysis reveals new species groupings in the genus Colletotrichum. Experimental Mycology 18: 121-138.
Shimizu, K., C. Tanaka, Y. L. Peng and M. Tsuda. 1998. Phylogeny of Bipolaris inferred from nucleotide sequences of Brn1, a reductase gene involved in melanin biosynthesis. The Journal of General and Applied Microbiology 44: 251-258.
Silva-Mann, R., M. G. G. C. Vieira, J. C. Machado, J. R. Bernardino Filho, K. C. C. Salgado and M. R. Stevens. 2005. AFLP markers differentiate isolates of Colletotrichum gossypii from C. gossypii var. cephalosporioides. Fitopatologia Brasileira 30: 169-172.
Silva, D. N., P. Talhinhas, V. Várzea, L. Cai, O. S. Paulo and D. Batista. 2012. Application of the Apn2/MAT locus to improve the systematics of the Colletotrichum gloeosporioides complex: an example from coffee (Coffea spp.) hosts. Mycologia 104: 396-409.
Sreenivasaprasad, S., A. E. Brown and P. Mills. 1992. DNA sequence variation and interrelationships among Colletotrichum species causing strawberry anthracnose. Physiological and Molecular Plant Pathology 41: 265-281.
Sreenivasaprasad, S., B. Meehan, P. Mills and A. Brown. 1996. Phylogeny and systematics of 18 Colletotrichum species based on ribosomal DNA spacer sequences. Genome 39: 499-512.
Sreenivasaprasad, S., K. Sharada, A. Brown and P. Mills. 1996. PCR‐based detection of Colletotrichum acutatum on strawberry. Plant Pathology 45: 650-655.
Sreenivasaprasad, S. and P. Talhinhas. 2005. Genotypic and phenotypic diversity in Colletotrichum acutatum, a cosmopolitan pathogen causing anthracnose on a wide range of hosts. Molecular Plant Pathology 6: 361-378.
Stamatakis, A., P. Hoover and J. Rougemont. 2008. A rapid bootstrap algorithm for the RAxML Web servers. Systematic Biology 57: 758-771.
Stenger, D. A., J. D. Andreadis, G. J. Voraa and J. J. Pancrazio. 2002. Potential applications of DNA microarrays in biodefense-related diagnostics. Current Opinion in Biotechnology 13: 208-212.
Sun, G., S. Oide, E. Tanaka, K. Shimizu, C. Tanaka and M. Tsuda. 2003. Species separation in Curvularia "geniculata" group inferred from Brn1 gene sequences. Mycoscience 44: 239-244.
Sutton, B., J. Bailey and M. Jeger. 1992. The genus Glomerella and its anamorph Colletotrichum. In Colletotrichum: Biology, Pathology and Control (J. A. Bailey and M. J. Jeger, eds) p.1-26 CABI, Wallingford, UK.
Sutton, B. C. 1980. The Coelomycetes, Fungi Imperfecti with Pycnidia, Acervuli and Stromata. CABI, Kew, UK.
Takano, Y., Y. Kubo, K. Shimizu, K. Mise, T. Okuno and I. Furusawa. 1995. Structural analysis of PKS1, a polyketide synthase gene involved in melanin biosynthesis in Colletotrichum lagenarium. Molecular and General Genetics 249: 162-167.
Talhinhas, P., S. Sreenivasaprasad, J. Neves-Martins and H. Oliveira. 2002. Genetic and morphological characterization of Colletotrichum acutatum causing anthracnose of lupins. Phytopathology 92: 986-996.
Tamura, K., J. Dudley, M. Nei and S. Kumar. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24: 1596-1599.
Than, P., R. Shivas, R. Jeewon, S. Pongsupasamit, T. Marney, P. Taylor and K. Hyde. 2008. Epitypification and phylogeny of Colletotrichum acutatum JH Simmonds. Fungal Diversity 28: 97-108.
Thompson, J. D., D. G. Higgins and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673-4680.
Tsuji, G., Y. Kenmochi, Y. Takano, J. Sweigard, L. Farrall, I. Furusawa, O. Horino and Y. Kubo. 2000. Novel fungal transcriptional activators, Cmr1p of Colletotrichum lagenarium and Pig1p of Magnaporthe grisea, contain Cys2His2 zinc finger and Zn (II) 2Cys6 binuclear cluster DNA‐binding motifs and regulate transcription of melanin biosynthesis genes in a developmentally specific manner. Molecular Microbiology 38: 940-954.
Ureña-Padilla, A., S. MacKenzie, B. Bowen and D. Legard. 2002. Etiology and population genetics of Colletotrichum spp. causing crown and fruit rot of strawberry. Phytopathology 92: 1245-1252.
Vinnere, O., J. Fatehi, S. A. Wright and B. Gerhardson. 2002. The causal agent of anthracnose of Rhododendron in Sweden and Latvia. Mycological Research 106: 60-69.
Von Arx. 1957. Die Arten der Gattung Colletotrichum Cda. Phytopath. Z. 29: 413-468.
Weir, B., P. Johnston and U. Damm. 2012. The Colletotrichum gloeosporioides species complex. Studies in Mycology 73: 115-180.
Yang, Y., Z. Liu, L. Cai, K. Hyde, Z. Yu and E. McKenzie. 2009. Colletotrichum anthracnose of Amaryllidaceae. Fungal Diversity 39: 123-146.
Zhou, J. 2003. Microarrays for bacterial detection and microbial community analysis. Current Opinion in Microbiology 6: 288-294.
Zhu, H. and M. Snyder. 2003. Protein chip technology. Current Opinion in Chemical Biology 7: 55-63.

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