(44.192.66.171) 您好!臺灣時間:2021/05/18 00:54
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:楊颺
研究生(外文):Yang Yang
論文名稱:臺灣一葉蘭‘梅雪’與‘楓星’之微體繁殖
論文名稱(外文):Micropropagation of Pleione formosana ‘Mei Snow’ and ‘Feng Star’
指導教授:葉德明葉德明引用關係
指導教授(外文):Der-Ming Yeh
口試委員:沈榮壽朱建鏞張耀乾
口試委員(外文):Rong-Show ShenChien-Young ChuYao-Chien Chang
口試日期:2013-06-25
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:園藝暨景觀學系
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:121
中文關鍵詞:組織培養體胚發生發光二極體
外文關鍵詞:tissue culturesomatic embryogenesislight-emitting diode
相關次數:
  • 被引用被引用:1
  • 點閱點閱:301
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
臺灣一葉蘭(Pleione formosana Hayata)是臺灣原生的落葉性蘭花,球莖運輸便利極具外銷日本歐美市場潛力,現只臺大山地實驗農場擁有品種權,但雖有優良之商業品種,受限於繁殖倍率低而無法大量供應外銷及內銷市場。為快速大量生產臺灣一葉蘭商業品種並維持品種特性,本研究採新品種‘梅雪’與‘楓星’,取冷藏過的球莖芽體,以組織培養誘導體胚或不定芽,其後探討不同培養基及光源條件對其增殖生長的影響,調整培養基中生長調節劑之種類與濃度,以提高次級培植體繼代後的增殖率,並調查不同培養光源與光度對組培苗生長之影響。最後調整組培苗出馴化期間的光源與光度,期望能提高移植後的存活率與生長量,並探討臺灣一葉蘭組培苗的出瓶適期。
臺灣一葉蘭‘梅雪’球莖於0、2、4、6、8、10、12、15℃黑暗生長箱中冷藏4、6、8週後,取第四芽培養於添加20 g‧L-1 sucrose、4 g‧L-1 gelrite、0.15 mg∙L-1 NAA、0.2 mg∙L-1 BA之1/2 Murashige & Skoog (MS)培養基,以2-8℃冷藏6週者在培養24週後有較高的增殖率。取5℃冷藏6週的‘梅雪’球莖頂芽、第四芽及第五芽為培植體,僅第四芽與第五芽培養於含20 g‧L-1 sucrose、3 g‧L-1 Tryptone、4 g‧L-1 gelrite及2 g‧L-1活性碳的1/2 MS培養基後可增殖,並添加1.2、2.4 mg‧L-1 Dicamba或1.1、2.2 mg‧L-1 Picloram組合0.2 mg‧L-1 BA可誘導直接體胚發生。
將臺灣一葉蘭‘梅雪’及‘楓星’第四芽培養再生之叢生芽,分切培養於含不同濃度NAA及BA之1/2 MS中,培養8週後以0.2 mg‧L-1 NAA組合0.4 mg‧L-1 BA處理誘導的體胚數最多,誘導出的體胚與無菌播種的臺灣一葉蘭小苗有相似的發育過程,體胚繼代至含0、0.2、0.4 mg‧L-1 NAA組合 BA的1/2 MS培養基,可從初級體胚表面產生次級體胚,次級體胚與初級體胚有相似的發育過程,NAA濃度越高越能促進體胚發育成小苗,體胚發育成的小苗經埋蠟切片可觀察到獨立封閉的維管束構造。取臺灣一葉蘭‘梅雪’與‘楓星’組培苗分切為葉尖、葉基及球莖薄片進行培養,僅帶有芽體的球莖薄片可增殖,適用的培養基為添加0.2 mg∙L-1 2, 4-D或NAA 組合0.2 mg∙L-1 BA之1/2 MS培養基。
培養於LED白光(19% 紅 + 52% 綠 + 29% 藍)下的組培苗,較LED紅光、LED藍光、LED紅藍混合光及螢光燈有高的芽數、鮮重、球莖寬度與葉長。適合臺灣一葉蘭組培苗的培養光度為30 μmol‧m-2‧s-1。組培苗出瓶後在光度30、60及90 μmol‧m-2‧s-1下馴化兩週期間,雖然30 μmol‧m-2‧s-1處理的Fv/Fm值及淨光合作用速率較高,但移植後60及90 μmol‧m-2‧s-1處理有較高的存活率及生長量。組培苗出瓶後於60 μmol∙m-2∙s-1不同光質下馴化3週,以LED白光(19% red + 52% green + 29% blue)處理者在馴化期間有較高的淨光合作用速率值,且移植後的存活率和生長量亦較高。將瓶內培養8、12、16週已結球並帶根的組培苗出瓶,之後於光度60 μmol‧m-2‧s-1下馴化3週並移植,以培養12、16週者有較高的移植後存活率、鮮重、球莖寬度與根數。


Pleione formosana Hayata is a Taiwan-native deciduous orchid species suitable for transportation to meet European and Japanese market`s demand. Though cultivars have been developed but sales were limited due to low propagation efficiency. This study aimed to determine suitable conditions for mass propagation of P. formosana ‘Mei Snow’ and ‘Feng Star’ by somatic embryogenesis or multiple shooting. Bud from cold stored P. formosana corms were taken as explants, and cultured on mediums containing various plant growth regulator combinations. Light intensity and quality during in vitro and ex vitro acclimatization were tested to increase growth and ex vitro survival. The effect of in vitro cultured duration on ex vitro growth was also studied.
The fourth bud on P. formosana corms after cold storage at 0, 2, 4, 6, 8, 10, 12, and 15oC for 4, 6, and 8 weeks were taken as explants. The fourth bud explants were cultured on 1/2 Murashige & Skoog (MS) medium containing 20 g‧L-1 sucrose, 4 g‧L-1 gelrite, 0.15 mg∙L-1 NAA (naphthalene acetic acid), and 0.2 mg∙L-1 BA (benzyl adenine purine). Those stored at 2-8oC for 6 weeks showed higher multiplication rate when cultured after 24 weeks. Apical bud, the fourth bud, and the fifth bud of P. formosana ‘Mei Snow’ stored at 5oC for 6 weeks were taken as explants. Only the fourth bud and the fifth bud explants were able to regenerate after cultured on 1/2 MS medium containing 20 g‧L-1 sucrose, 3 g‧L-1 Tryptone, 4 g‧L-1 gelrite, and 2 g‧L-1 activated charcoal. Direct somatic embryo were induced when the fourth and the fifth bud explants were cultured on 1/2 MS medium supplemented with 1.2, 2.4 mg‧L-1 Dicamba or 1.1, 2.2 mg‧L-1 Picloram and 0.2 mg‧L-1 BA.
Multiple shooting regenerated from the fourth bud culture were divided as secondary explants, and cultured for 8 weeks on 1/2 MS medium supplemented with NAA and BA combinations. Results showed those cultured on 0.2 mg‧L-1 NAA combined 0.4 mg‧L-1 BA induced more somatic embryos, with growth phase similar to seed-develop plantlets. Secondary somatic embryos were induced from surface of primary somatic embryos after subcultured to 1/2 MS medium containing 0, 0.2, and 0.4 mg‧L-1 NAA and BA combinations. Secondary somatic embryos shared similar growth stage with primary somatic embryos. Somatic embryos tended to develop plantlets with increasing NAA concentration. Anatomical reservation showed that plantlets developed from somatic embryo had independent isolated vascular bundle. Leaf tip, leaf base, and corm slice from in vitro plantlets were also taken as explants, but only corm slices with buds could regenerate to plantlets. More somatic embryos were induced from those cultured on 1/2 MS medium supplemented with 0.2 mg∙L-1 2, 4-D or NAA combined with 0.2 mg∙L-1 BA.
Plantlets cultured with LED (light emitting diode) white light (19% red + 52% green + 29% blue) had more buds, fresh weight, corm diameter, and leaf length than those cultured with LED red, LED blue, LED red plus blue light, and fluorescent lamp. Suitable light intensity for in vitro culture was 30 μmol‧m-2‧s-1 PPF. Ex vitro plantlets were acclimatized under 30, 60, and 90 μmol‧m-2‧s-1 PPF for 2 weeks. Results showed that plantlet acclimatized under 30 μmol‧m-2‧s-1 showed higher Fv/Fm value and net photosynthesis, but those acclimatized under 60 and 90 μmol‧m-2‧s-1 had higher survival rate and better growth after transplanting. En vitro plantlets were also acclimatized under various light quality at 60 μmol‧m-2‧s-1 for 3 weeks. Results showed that plantlets under LED white light had higher net photosynthesis, survival percentage, and more growth after transplanting. Plantlets cultured in vitro after 8, 12, and 16 weeks were transplanted and acclimatized under 60 μmol‧m-2‧s-1 for 3 weeks. Results showed that higher survival rate, fresh weight, corm diameter, and root number were recorded in plantlets cultured for 12 and 16 weeks then that cultured for 8 weeks.


生長調節劑縮寫表………………………………………………………………………i
摘要……………………………………………………………………………………ii
Abstract…………………………………………………………………………………iv
目錄……………………………………………………………………………………..vi
表目錄…………………………………………………………………………………viii
圖目錄…………………………………………………………………………………ix
前言(Introduction)……………………………………………………………………….1
前人研究(Literature Review)……………………………………………………………3
一、臺灣一葉蘭之形態與產業概況………………………………………………....3
(一)臺灣一葉蘭之形態與生長…………………………………………………….3
(二)產業與品種概況………………………………………………………………4
(三)臺灣一葉蘭之繁殖…………………………………………………………...4
二、花序培養不定芽再生…………………………………………………………….6
(一)花序培植體…………………………………………………………………….7
(二)生長調節劑…………………………………………………………………….8
三、體胚發生………………………………………………………………………….8
(一)球根花卉之體胚發生………………………………………………………...9
(二)蘭花之體胚發生…………………………………………………………….11
四、光對組培苗生長與出瓶馴化之影響…………………………………………16
(一)光度...…………………………………………………………………………16
(二)光源…………………………………………………………………………...17
材料與方法(Materials and Methods)………………………………………………….19
試驗一、冷藏溫度與時間對臺灣一葉蘭芽體再生之影響………………………...19
試驗二、BA與NAA濃度對臺灣一葉蘭體胚發生之影響………………………...20
試驗三、生長調節劑對臺灣一葉蘭體胚發生與成熟之影響……………………..20
試驗四、培植體與生長調節劑對臺灣一葉蘭‘梅雪’體胚發生之影響……………21
試驗五、生長調節劑對臺灣一葉蘭葉片與球莖薄片體胚發生之影響…………..24
試驗六、光質對臺灣一葉蘭組培瓶苗生育之影響…………………………….. …25
試驗七、光度對臺灣一葉蘭組培瓶苗生育之影響…………………………….. …25
試驗八、出瓶馴化之光強度對臺灣一葉蘭組培苗光合作用及生長之影響……..26
試驗九、出瓶馴化之光質對臺灣一葉蘭組培苗光合作用及生長之影響………...27
試驗十、瓶內培養時間對臺灣一葉蘭組培苗出瓶後生長之影響………………..28
結果(Results)…………………………………………………………………………...30
試驗一、冷藏溫度與時間對臺灣一葉蘭芽體再生之影響………………………..30
試驗二、BA與NAA濃度對臺灣一葉蘭體胚誘導之影響………………………30
試驗三、生長調節劑對臺灣一葉蘭體胚發生與成熟之影響……………………..31
試驗四、培植體與生長調節劑對臺灣一葉蘭‘梅雪’體胚發生之影響……………32
試驗五、生長調節劑對臺灣一葉蘭葉片與球莖薄片體胚發生之影響…………...33
試驗六、光質對臺灣一葉蘭組培瓶苗生育之影響………………………………..34
試驗七、光度對臺灣一葉蘭組培瓶苗生育之影響………………………………..35
試驗八、出瓶馴化之光強度對臺灣一葉蘭組培苗光合作用及生長之影響……..35
試驗九、出瓶馴化之光質對臺灣一葉蘭組培苗光合作用及生長之影響………..36
試驗十、瓶內培養時間對臺灣一葉蘭組培苗出瓶後生長之影響………………..37
討論(Discussion)……………………………………………………………………….79
試驗一、冷藏溫度與時間對臺灣一葉蘭芽體再生之影響………………………..79
試驗二、BA與NAA濃度對臺灣一葉蘭體胚發生之影響……………………….81
試驗三、生長調節劑對臺灣一葉蘭體胚發生與成熟之影響……………………...84
試驗四、培植體與生長調節劑對臺灣一葉蘭‘梅雪’體胚發生之影響……………86
試驗五、生長調節劑對臺灣一葉蘭葉片與球莖薄片體胚發生之影響…………..90
試驗六、光質對臺灣一葉蘭組培瓶苗生育之影響………………………………..92
試驗七、光度對臺灣一葉蘭組培瓶苗生育之影響………………………………..94
試驗八、出瓶馴化之光強度對臺灣一葉蘭組培苗光合作用及生長之影響……..95
試驗九、出瓶馴化之光質對臺灣一葉蘭組培苗光合作用及生長之影響………..98
試驗十、瓶內培養時間對臺灣一葉蘭組培苗出瓶後生長之影響……………….101
結論(Conclusions……………………………………………………………………..105
參考文獻(References)………………………………………………………………...108
附錄…………………………………………………………………………………...119



方煒. 2011. 完全控制型植物工廠. 豐年社. 臺北. p.978-957.
王美琴. 2007. 石蒜屬雙核型雜種花序培養、增殖與多倍體誘導. 國立臺灣大學園藝學系碩士論文. 臺北
朱欽昌、李哖. 1984. 臺灣一葉蘭之芽頂培養. 中國園藝 31:275(摘要).
李哖. 1984. 臺灣一葉蘭之生長習性與生產. 臺灣農業試驗所特刊14:53-54.
李哖. 1987. 臺灣一葉蘭之無菌播種. 桃園農改場花卉生產改進研討會專輯.
p:135-147.
李哖. 1995. 蘭科植物, p.665-684. 農家要覽農作篇(二). 豐年社. 臺北.
曹燕慧. 2004. 石蒜屬雙核型雜種幼花培養之植株再生. 國立臺灣大學園藝學系 碩士論文. 臺北.
陳姿翰、易美秀、魏芳明. 2011. 瓶苗出瓶適期對文心蘭幼苗生長之影響. 台中區 農業改良場研究彙報 112:15-24.
黃成林. 2004. 獨蒜蘭快繁技術之研究. 安徽農業大學學報 31:100-103.
賴建洲、饒瑞佶、方煒、張森富. 2004. 交流供電的紅光發光二極體應用於彩色
海芋組培苗量產栽培. 中華農學會報 5:493-502.
張耀乾. 1989. 臺灣一葉蘭之組織培養與報歲蘭根莖之分化. 國立臺灣大學園藝學系碩士論文. 臺北
張耀乾、李哖. 1992. 臺灣一葉蘭之組織培養. 中國園藝 38:80-90.
莊馥綱. 2004. 臺灣一葉蘭之微體繁殖. 國立臺灣大學園藝學系碩士論文. 臺北
莊錦華. 1983. 台灣一葉蘭無菌發芽與幼苗生長之研究. 國立臺灣大學園藝學系碩士論文. 臺北
莊錦華、李哖. 1983. 植物生長調節劑對臺灣一葉蘭種子發芽、幼苗生長及結球之影響. 中國園藝 29:315.
褚昱均、葉德銘. 2006a. 馴化光度對白馬粗肋草組織培養苗出瓶後葉片光合作用與植株生長之影響. 臺灣園藝 52:199-208.
褚昱均、葉德銘. 2006b. 光度對黛粉葉組織培養苗出瓶後生長與光合作用之影響. 中華農學會報 7:404-413.
劉美蓉. 1982. 臺灣一葉蘭之分化與生長. 國立臺灣大學園藝學系碩士論文. 臺北
滕蕙蘭. 1985. 台灣一葉蘭休眠生理之研究. 國立臺灣大學園藝系碩士論文. 臺北
滕蕙蘭、李哖、蔡牧起. 1985. 球莖熟度、貯溫與貯期對台灣一葉蘭開花與碳水化合物含量之影響. 中國園藝 31:174-187.
盧美君. 2003. 臺灣一葉蘭生產技術. 農業世界 241:50-53.
盧美君. 2004. 利用組織培養繁殖臺灣一葉蘭. 植物種苗 6:61-72.
Ahouran, M., R. Hosseini, and R. Zarghami. 2012. Corms as a source of explants for the successful clonal propagation of Crocus cancellatus. J. Crop Sci. Biotech. 15:47-51.
Bach, A. 1992. Somatic embryogenesis from zygotic embryos and meristems of Freesia hybrida. Acta Hort. 325:429-43.
Bach, A. and A. Ptak. 2001. Somatic embryogenesis and plant regeneration from ovaries of Tulipa gesneriana L. in vitro cultures. Acta Hort. 560:391-394.
Bakhshaie, M., M. Babalar, M. Mirmasoumi, and A. Khalighi. 2010. Somatic embryogenesis and plant regeneration of Lilium ledebourii (Baker) Boiss, an endangered species. Plant Cell Tiss. Org. Cult. 102:229-235.
Bao, Y., G. Liu, X. Shi, W. Xing, G. Ning, J. Liu, and M. Bao. 2012. Primary and repetitive secondary somatic embryogenesis in Rosa hybrida ‘Samantha’. Plant Cell Tiss. Org. Cult. 109:411-418.
Bian, F.H., F.N. Qu, C.X. Zheng, C.R. You, and X.Q. Gong. 2007. Recent advances in Cyclamen persicum Mill. somatic embryogenesis. North. Hort. 8:70-72.
Blazquez, S., E. Olmos, J. Hernandez, N. Fernandez-Garcia, J. Fernandez, and A. Piqueras. 2009. Somatic embryogenesis in saffron (Crocus sativus L.). Histological differentiation and implication of some components of the antioxidant enzymatic system. Plant Cell Tiss. Org. Cult. 97:49-57.
Bartoli, C.G., E.A. Tambussi, F. Diego, and C.H. Foyer. 2009. Control of ascorbic acid synthesis and accumulation and glutathione by the incident light red/far red ratio in Phaseolus vulgaris leaves. FEBS Letters 583:118-122.
Chen, J.J., M.C. Liu, and Y.H. Ho. 2000. Size of in vitro plantlets affects subsequent tuber production of acclimated calla lily. HortScience 35:290-292.
Chen, J.T. and W.C. Chang. 2002. Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis in Oncidium ‘Gower Ramsey’. Plant Cell Tiss. Org. Cult. 69:41-44.
Chen, J.T. and W.C. Chang. 2005. TIBA affects the induction of direct somatic embryogenesis from leaf explants of Oncidium. Plant Cell Tiss. Org. Cult. 79:315-320.
Chen, J.T. and W.C. Chang. 2006. Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis. Biol. Plant. 50:169-173.
Chen, J.T., C. Chang, and W.C. Chang. 1999. Direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey and subsequent plant regeneration. Plant Cell Rpt. 19:143-149.
Chen, T.Y., J.T. Chen, and W.C. Chang. 2004. Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids. Plant Cell Tiss. Org. Cult. 76:11-15
Chung, H., J.T. Chen, and W.C. Chang. 2007. Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium. Biol. Plant. 51:346-350.
Cui, K.R. and R.L. Dai. 2000. Molecular biology of plant somatic embryogenesis. Sci. Press, Beijing. p.48-54.
Demeter, Z., G. Suranyi, V. Molnar, G. Sramko, D. Beyer, Z. Konya, G. Vasas, M. M-Hamvas, and C. Mathe. 2010. Somatic embryogenesis and regeneration from shoot primordia of Crocus heuffelianus. Plant Cell Tiss. Org. Cult. 100:349-353.
Duquenne, B., T. Eeckhaut, S. Werbrouck, and J. Van Huylenbroeck. 2006. In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids. Plant Cell Tiss. Org. Cult. 87:329-331.
Finer, J.J. 1987. Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.) on a high sucrose-containing medium. Plant Cell Rpt. 6:372-374.
Fondy, B.R., D.R. Geiger, and J.C. Servaites. 1989. Photosynthesis, carbohydrate metabolism and export in Beta vulgaris L. and Phaseolus vulgaris L. during square and sinusoidal light regimes. Plant Physiol. 89:396–402.
Gao, X., D. Yang, D. Cao, M. Ao, X. Sui, Q. Wang, J. Kimatu, and L. Wang. 2010. In vitro micropropagation of Freesia hybrida and the assessment of genetic and epigenetic stability in regenerated plantlets. J. Plant Growth Regulat. 29:257-267.
George, E.F., M.A. Hall, and G.J.D. Klerk. 2008. Somatic embryogenesis, p.335-354. In: E. F. George, M. A. Hall and G.J. D. Klerk (eds.). Plant propagation by tissue culture Springer, Netherlands.
Gow, W.P., J.T. Chen, and W.C. Chang. 2010. Enhancement of direct somatic embryogenesis and plantlet growth from leaf explants of Phalaenopsis by adjusting culture period and explant length. Acta Physiol. Plant. 32:621-627.
Grout, B.W. and S. Millam. 1985. Photosynthetic development of micropropagated strawberry plantlets following transplanting. Ann. Bot. 55: 129-131.
Guo, Y. and Z. Zhang. 2005. Establishment and plant regeneration of somatic embryogenic cell suspension cultures of the Zingiber officinale Rosc. Scientia Hort. 107:90-96.
Huang, C.H. and J.P. Chung. 2011. Efficient indirect induction of protocorm-like bodies and shoot proliferation using field-grown axillary buds of Lycaste hybrid. Plant Cell Tiss. Org. Cult. 106:31-38.
Huang, X.L. and Y.J. Li. 1995. Morphogenesis and regulation of higher plant organ culture in vitro. Sci. Press, Beijing. p.46-72.
Ji, A., X. Geng, Y. Zhang, H. Yang, and G. Wu. 2011. Advances in somatic embryogenesis research of horticultural plants. Amer. J. Plant Sci. 2:727-732.
Kadleček, P., I. Ticha, D. Haisel, V. Čapkova, and C. Schafer. 2001. Importance of in vitro pretreatment for ex vitro acclimatization and growth. Plant Sci. 161:695-701.
Karamian, R. 2004. Plantlet regeneration via somatic embryogenesis in four species of Crocus. Acta Hort. 650:253-259.
Karamian, R. and H. Ebrahimzadeh. 2001. Plantlet regeneration from protoplast-derived embryogenic calli of Crocus cancellatus. Plant Cell Tiss. Org. Cult. 65:115-121.
Karaoğlu, C., S. Cocu, A. İpek, I. Parmaksız, S. Uranbey, E. Sarıhan, N. Arslan, M.D. Kaya, C. Sancak, S. Ozcan, B. Gurbuz, S. Mirici, C. Er, and K.M. Khawar. 2007. In vitro micropropagation of saffron. Acta Hort. 739:223-227.
Koksal, N., H. Gulen and A. Eris. 2010. Total soluble sugars in tulip bulbs and freesia corms during storage. Acta Hort. 877:1791-1798.
Kuo, H.L., J.T. Chen, and W.C. Chang. 2005. Efficient plant regeneration through direct somatic embryogenesis from leaf explants of Phalaenopsis ‘Little Steve’. In Vitro Cell. Dev. Biol. Plant. 41:453-456.
Kulpa, D. 2011. Plant regeneration in inflorescence culture of chrysanthemum [Dendranthema ×grandiflora (Ramat.) Kitamura]. J. Food Agr. Environ. 9:715-718.
Lambrechts, H., F. Rook, and C. Kolloffel. 1994. Carbohydrate status of Tulip bulbs during cold-induced flower stalk elongation and flowering. Plant Physiol. 104:515-520.
Lan, T., P. Hong, C. Huang, W. Chang, and C. Lin. 2009. High-frequency direct somatic embryogenesis from leaf tissues of Drimiopsis kirkii Baker (giant squill). In Vitro Cell. Dev. Biol. Plant. 45:44-47.
Lang, N.T. and N.T. Hang. 2006. Using biotechnological approaches for Vanda orchid improvement. OmonRice. 14:140-143.
Laublin, G., H.S. Saini, and M. Cappadocia. 1991. In vitro plant regeneration via somatic embryogenesis from root culture of some rhizomatous irises. Plant Cell Tiss. Org. Cult. 27:15-21.
Lee, S.H., R. Tewari, E.J. Hahn, and K.Y. Paek. 2007. Photon flux density and light quality induce changes in growth, stomatal development, photosynthesis and transpiration of Withania somnifera (L.) Dunal. plantlets. Plant Cell Tiss. Org. Cult. 90:141-151.
Li, B., D.R Geiger, and W.J. Shieh. 1992. Evidence for circadian regulation of starch and sucrose synthesis in sugar beet leaves. Plant Physiol. 99:1393-1399.
Li, X., S. Krasnyanski, and S.S. Korban. 2002. Somatic embryogenesis, secondary somatic embryogenesis, and shoot organogenesis in Rosa. Plant Physiol. 159:313-319.
Lian, M.L., H.N. Murthy, and K.Y. Paek. 2002. Culture method and photosynthetic photon flux affect photosynthesis, growth and survival of Limonium ‘Misty Blue’ in vitro. Scientia. Hort. 95:239-249.
Lian, M.L., H.N. Murthy, and K.Y. Paek. 2003. Photoautotrophic culture conditions and photosynthetic photon flux influence growth of Lilium bulblets in vitro. In Vitro Cell. Dev. Biol. Plant. 39:532-535.
Liao, Y.J., Y.C. Tsai, Y.W. Sun, R.S. Lin, and F.S. Wu. 2011. In vitro shoot induction and plant regeneration from flower buds in Paphiopedilum orchids. In Vitro Cell. Dev. Biol. Plant. 47:702-709.
Little, E.L., Z.V. Magbanua, and W.A. Parrott. 2000. A protocol for repetitive somatic embryogenesis from mature peanut epicotyls. Plant Cell Rpt. 19:351-357.
Lu, M.J. 2004. High frequency plant regeneration from callus culture of Pleione formosana Hayata. Plant Cell Tiss. Org. Cult. 78:93-96.
Malik, M. 2008. Comparison of different liquid/solid culture systems in the production of somatic embryos from Narcissus L. ovary explants. Plant Cell Tiss. Org. Cult. 94:337-345.
Mayer, J., G. Stancato, and B. Appezzato-Da-Gloria. 2010. Direct regeneration of protocorm-like bodies (PLBs) from leaf apices of Oncidium flexuosum Sims (Orchidaceae). Plant Cell Tiss. Org. Cult. 103:411-416.
Sun, M., X.F. Li, J. Kong, J.F. Shi, and X.Q. Zhang. 2012. Research on somatic embryogenesis in Lilium longiflorum ‘White Heaven’. Acta Hort. 937:589-594.
Mujib, A., S. Banerjee, and P. Ghosh. 2006. Origin, development and structure of somatic embryos in selected bulbous ornamentals: BAP as inducer. Somatic embryogenesis, p.15-24. In: A. Mujib and J. Šamaj (eds.). Vol. 2. Springer, Berlin.
Nasim, S.A., A. Mujib, R. Kapoor, S. Fatima, J. Aslam, and Mahmooduzzafar. 2010. Somatic embryogenesis in Allium sativum L. (cv. Yamuna Safed 3): Improving embryo maturation and germination with PGRs and carbohydrates. Anal. Bio. 32:1-9.
Nhut, D.T., B. Van Le, N.T. Minh, J.T. de Silva, S. Fukai, M. Tanaka, and K. Tran Thanh Van. 2002. Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum. Plant Growth Regulat. 37:193-198.
Nhut, D., T. Takamura, H. Watanabe, K. Okamoto, and M. Tanaka. 2003. Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell Tiss. Org. Cult. 73:43-52.
Outlaw, W.H., Jr. and X.D. Vlieghere-He. 2001. Transpiration rate. An important factor controlling the sucrose content of the guard cell apoplast of broad bean. Plant Physiol. 126:1716-1724.
Park, S.Y., H.N. Murthy, and K.Y. Paek. 2003. Protocorm-like body induction and subsequent plant regeneration from root tip cultures of Doritaenopsis. Plant Sci. 164:919-923.
Ptak, A. 2010. Somatic embryogenesis in in vitro culture of Leucojum vernum L., p:223-233. In: S.M. Jain and S.J. Ochatt (eds.). Protocols for in vitro propagation of ornamental plants, Methods in molecular biology. Vol. 589. Humana Press, New York.
Ptak, A. and A. Bach. 2007. Somatic embryogenesis in tulip (Tulipa gesneriana L.) flower stem cultures. In Vitro Cell. Dev. Biol. Plant 43:35-39.
Raja, W., G. Zaffer, and S.A. Wani. 2007. In vitro microcorm formation in saffron (Crocus sativus l.). Acta Hort. 739:291-296.
Remotti, P.C. 1995. Primary and secondary embryogenesis from cell suspension cultures of Gladiolus. Plant Sci. 107:205-214.
Roy, J., S. Naha, M. Majumdar, and N. Banerjee. 2007. Direct and callus-mediated protocorm-like body induction from shoot-tips of Dendrobium chrysotoxum Lindl. (Orchidaceae). Plant Cell Tiss. Org. Cult. 90:31-39.
Sage, D.O., J. Lynn, and N. Hammatt. 2000. Somatic embryogenesis in Narcissus pseudonarcissus cvs. Golden Harvest and St. Keverne. Plant Sci. 150:209-216.
Sheibani, M., S.H. Nemati, G.H. Davarinejad, A.V. Azghandi, and A.A. Habashi. 2007. Induction of somatic embryogenesis in saffron using thidiazuron (TDZ). Acta Hort. 739:259-267.
Shin, K.S., H.N. Murthy, J.W. Heo, E.J. Hahn, and K.Y. Paek. 2008. The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants. Acta Physiol. Plant. 30:339-343.
Shibli, R. and M.M. Ajlouni. 2000. Somatic embryogenesis in the endemic black iris. Plant Cell Tiss. Org. Cult. 61:15-21.
Shibli, R., M. Duwayri, J. Sawwan, M. Shatnawi, and T. Al-Qudah. 2012. Regeneration via somatic embryogenesis of the endangered wild arum (Arum palaestinum ). In Vitro Cell. Dev. Biol. Plant. 48:335-340.
Saez, P.L., L.A. Bravo, M.I. Latsague, M.E. Sanchez, and D.G. Rios. 2012. Increased light intensity during in vitro culture improves water loss control and photosynthetic performance of Castanea sativa grown in ventilated vessels. Scientia Hort. 138:7-16.
Sivanesan, M., Y. Lim, E.H. Jo, and B.R. Jeong. 2011. Somatic embryogenesis and plant regeneration in Crocus vernus. 2nd International conference on environmental science and development. Vol.4. IACSIT Press, Singapore.
Stefaniak, B. 1994. Somatic embryogenesis and plant regeneration of Gladiolus. Plant Cell Rpt. 13:386-389.
Tribulato, A. and F. Branca. 2011. Somatic embryogenesis from flower pedicels of oriental lilies. Acta Hort. 900:369-376.
Teixeira Da Silva, J., D. Giang, and M. Tanaka. 2006. Photoautotrophic micropropagation of Spathiphyllum. Photosynthetica 44:53-61.
Van Huylenbroeck, J.M., A. Piqueras, and P.C. Debergh. 1998. Photosynthesis and carbon metabolism in leaves formed prior and during ex vitro acclimatization of micropropagated plants. Plant Sci. 134:21-30.
Van Huylenbroeck, J.M., A. Piqueras and P.C. Debergh. 2000. The evolution of photosynthetic capacity and the antioxidant enzymatic system during acclimatization of micropagated Calathea plants. Plant Sci. 155:59-66.
von Arnold, S., I. Sabala, P. Bozhkov, J. Dyachok, and L. Filonova. 2002. Developmental pathways of somatic embryogenesis. Plant Cell Tiss. Org. Cult. 69:233-249.
Wang, L., X.M. Bao, Y.H. Liu, and S. Hao. 1994. Origin of direct somatic embryos from cultured inflorescence axis segments of Freesia refracta. Intl. J. Plant Sci. 155:672-676.
Wang, H., M. Gu, J. Cui, K. Shi, Y. Zhou, and J. Yu. 2009. Effects of light quality on CO2 assimilation, chlorophyll-fluorescence quenching, expression of Calvin cycle genes and carbohydrate accumulation in Cucumis sativus. J. Photochem. Photobio. Biol. 96:30-37.
Wulster, G.J. and T.J. Gianfagna. 1991. Freesia hybrida respond to ancymidol, cold storage of corms, and greenhouse temperatures. HortScience 26:1276-1278.
Xiao, Y., Y. Lok, and T. Kozai. 2003. Photoautotrophic growth of sugarcane plantlets in vitro as affected by photosynthetic photon flux and vessel air exchanges. In Vitro Cell. Dev. Biol. Plant. 39:186-192.
Xu, R.Y., Y. Niimi, and D.S. Han. 2006. Changes in endogenous abscisic acid and soluble sugars levels during dormancy-release in bulbs of Lilium rubellum. Scientia Hort. 111:68-72.
Yaacob, J.S., A.I.M. Yussof, R.M. Taha, and S. Mohajer. 2012. Somatic embryogenesis and plant regeneration from bulb, leaf and root explants of African blue lily (Agapanthus praecox ssp. minimus). Austra. J. Crop Sci. 6:1462-1470.
Zaidi, N., N. Habib, K.F. Zafar, and S.I. Zafar. 2000. Bulbous and cormous monocotyledonous ornamental plants in vitro. Science Vision 6(1):58-73.
Zhao, P., F. Wu, F.S. Feng, and W.J. Wang. 2008. Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Lindl. In Vitro Cell. Dev. Biol. Plant. 44:178-185.
Ziv, M. and H.L. Kipnis. 2000. Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro. Plant Cell Rpt. 19:845-850.



QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top