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研究生:王揭晴
研究生(外文):Chieh-Ching Wang
論文名稱:光強度、氮素與通氣處理對水稻幼苗氮素吸收及同化之影響
論文名稱(外文):Effects of Light Intensity, Nitrogen and Aeration on Nitrogen Uptake and Assimilation in Rice Seedlings
指導教授:王淑珍王淑珍引用關係
指導教授(外文):Su-Jen Wang
口試委員:葉開溫洪傳揚黃文理陳仁治
口試委員(外文):Kai-Wun YehChwan-Yang HongWen-Lii HuangJen-Chin Chen
口試日期:2013-07-26
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:農藝學研究所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:59
中文關鍵詞:光強氮素吸收氮素同化通氣水稻
外文關鍵詞:light intensitynitrogen uptakenitrogen assimilationaerationrice
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氮素為植物最重要的營養元素之一,氮素同化對於作物發育及產量有極大的影響,本研究之主要目的為了解不同光強度對於水稻幼苗氮素吸收及同化的影響,並進一步探究光強度與養液氮素於調控氮源轉運蛋白及氮素同化酵素活性之交感作用,以及水耕液通氣處理對水稻幼苗氮素同化之影響。將催芽3天後的水稻幼苗置入生長箱中並處理不同光強度至第12天,研究結果顯示,高光強 (450 μmol m-2 s-1) 下生長的水稻幼苗相較於低光強 (50 μmol m-2 s-1) 處理生長勢較佳、銨硝態氮吸收效率上升、根部屬於高親和轉運系統之硝酸轉運蛋白OsNRT2.1、OsNRT2.2及銨轉運蛋白OsAMT1.1基因表現量上升、地上部及根部硝酸鹽還原酶 (Nitrate reductase; NR )和穀氨醯胺還原酶 (Glutamine synthetase; GS) 酵素活性上升、蛋白質及可溶性醣類含量增加。低光下養液外加蔗糖或2-OG之試驗發現,NR及GS活性皆會受誘導而增強。此外,高光強生長下之幼苗處理光合作用抑制劑DCMU時, NR和GS活性降低,如同低光強條件下生長之幼苗一般,而處理DCMU後再外加蔗糖則酵素活性會再受誘導而上升,因此推測高光強促進水稻幼苗氮素同化是透由光合產物(如:蔗糖)所調控。高光強下,缺氮處理會抑制NR和GS活性、降低蛋白質含量,缺氮下可溶性醣類分配至根部累積而促進根生長,但在低光強下氮濃度之影響不顯著。以銨態氮為單一氮源培養之水稻苗根系重量低於混合氮源栽培苗,而全硝酸態氮處理促進水稻根系延長,相較於銨或硝之單一氮源,混合氮對於根部NR及GS活性在高光強下具促進效果。此外,通氣處理可增進水稻幼苗對銨硝態氮之吸收及GS活性,進而促進生長。

誌謝......................................................i
中文摘要..................................................ii
Abstract................................................iii
目錄.....................................................iv
圖表目錄.................................................vii
附表目錄................................................viii
縮寫對照表................................................ix
前言
1.植物對氮素吸收同化之機制...................................1
2.光照影響氮代謝之機制......................................3
3.氮素對植物碳、氮代謝之影響.................................4
4.根系氧濃度對植物生長及氮代謝之影響..........................6
5.本論文之研究主題.........................................7
材料與方法
1.植物材料及栽培處理........................................8
2.光強度對水稻幼苗氮素吸收同化影響之分析.......................8
3.基因表現分析............................................13
4.蔗糖對水稻幼苗氮素同化酵素之影響............................15
5.2-OG對水稻幼苗氮素同化酵素之影響...........................15
6.光強變化對水稻幼苗氮素同化酵素之影響.........................15
7.不同光強下氮素對水稻幼苗氮素吸收及同化之交互影響...............16
8.通氣處理對水稻幼苗氮素吸收及同化之影響.......................16
結果
1.光強度對水稻幼苗生長、氮素吸收及同化之影響....................17
1.1光強度對水稻苗外觀形態及生長勢之影響........................17
1.2光強度對水稻苗銨、硝態氮吸收之影響..........................17
1.3不同光強度處理對水稻幼苗OsNRTs及OsAMTs之基因表現分析.........18
1.4光強度對水稻幼苗NR、GS酵素活性之影響.......................18
1.5光強度對水稻幼苗可溶性醣類含量與總蛋白含量之影響..............18
2.蔗糖對水稻幼苗氮素同化酵素活性之影響.........................19
2.1光強50 μmol m-2 s-1下外加蔗糖處理........................19
2.2光強450 μmol m-2 s-1下外加DCMU及蔗糖處理.................19
3.2-OG對水稻幼苗氮素同化酵素活性之影響影響.....................19
4.光強變化對水稻幼苗氮素同化酵素活性之影響......................20
5.不同光強下氮素濃度對水稻幼苗氮素吸收及同化之交互影響............20
5.1不同光強下氮素濃度對水稻幼苗生長勢之影響.....................20
5.2不同光強下氮素濃度處理對水稻幼苗NR與GS活性之影響………...........21
5.3不同光強下氮素濃度處理對水稻幼苗可溶性醣類及總蛋白質含量之影響...21
6.不同光強下氮源種類對水稻幼苗外觀形態及生長勢之影響..............21
6.1不同光強下氮源種類對水稻幼苗生長勢之影響.....................21
6.2不同光強下氮源種類對水稻幼苗銨硝態氮吸收效率之影響.............22
6.3不同光強下氮源種類對水稻幼苗NR、 GS活性、可溶性醣類含量與總蛋白含量之影響......................................................22
7.通氣處理對水稻幼苗生長、氮素吸收及同化之影響…..................22
7.1通氣處理對水稻幼苗外觀形態及生質量之影響.....................22
7.2通氣處理對銨硝態氮吸收效率、NR、GS活性、與總蛋白質的影響。......23
討論
1.光強度對水稻幼苗氮素吸收同化的影響與原因......................24
2.醣類及2-OG對氮素同化酵素之調控..............................24
3.光強與氮濃度交互作用對氮素同化的影響..........................25
4.光強與氮素形態交互作用對氮素同化的影響........................26
5.探討通氣處理增進植株生長及氮素同化之機制......................27
6.結語及未來展望...........................................28
參考文獻..................................................52

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