(3.238.130.97) 您好!臺灣時間:2021/05/13 23:40
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:陳嘉齡
研究生(外文):Chia-Ling Chen
論文名稱:醬油粕與芝麻粕添加對北蟲草固-液態培養生成生物活性物質產量影響之探討
論文名稱(外文):Effect of Addition of Soy Sauce Cake and Defatted Black Sesame Meal on Production of Bioactivity Compounds from Cordyceps militaris in Solid-Liquid Cultivation
指導教授:張耀南張耀南引用關係
指導教授(外文):Yaw-Nan Chang
學位類別:碩士
校院名稱:國立虎尾科技大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:70
中文關鍵詞:北蟲草紫米醬油粕芝麻粕生物活性物質
外文關鍵詞:Cordyceps militarisblack ricsoy sauce cakedefatted black sesame mealbioactivity compounds
相關次數:
  • 被引用被引用:0
  • 點閱點閱:322
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:1
本研究利用已萃取過花青素之紫米為主要基質,進行醬油粕與芝麻粕添加對北蟲草固-液態培養所生成生物活性物質(蟲草素、腺苷、蟲草酸及蟲草多醣)產量影響之探討,其中固態培養基質為100%:0%、75%:25%、50%:50%、25%:75%、0%:100%等5種不同比例之紫米與醬油粕或紫米與芝麻粕的組合,並在22℃與70%相對濕度下,以固-態態培養方式北蟲草60天。實驗結果顯示,僅有未含有任何粕之100%紫米組、含25%醬油粕之紫醬組及含25%芝麻粕之紫芝組等三組形成子實體,其子實體中蟲草素含量為含25%芝麻粕之紫芝組最高;腺苷、蟲草酸及蟲草多醣含量則為含25%醬油粕之紫醬組最高,由此可知醬油粕與芝麻粕添加有助於提高北蟲草子實體中蟲草素與腺苷含量,但僅有醬油粕添加有助於提高子實體中蟲草酸與蟲草多醣含量;然而,若兩者添加量超過25%含量則抑制北蟲草生成子實體。固態培養基中所含有最高蟲草素產量為50%芝麻粕之紫芝組,含有最高腺苷含量為50%醬油粕之紫醬組;但含有最高蟲草酸含量則為100%紫米組;除了100%芝麻粕組之蟲草多醣含量較低外,其餘組之含量並無明顯差異性。

After anthocyanins extraction, the extracted purple rice was used as the main solid medium for Cordyceps militaris cultivation in this study. The effect of addition of soy sauce cake and defatted black sesame meal on production of bioactivity compounds (cordycepin, adenosine, cordycepic acid and polysaccharides) from C. militaris in solid-liquid cultivation for 60 days at 22oC and 70% RH (relative humidity) was investigated. The mixture ratios of the solid media of purple rice (PR) and soy sauce cake (SSC) or PR and defatted black sesame meal (DBSM) were set as 100%:0%, 75%:25%, 50%:50%, 25%:75%, 0%:100% (defined as 100%PR (or PR), 25%, 50%, 75%, 100%SSC or DBSM, respectively). The results showed that C. militaris could grow to form its fruiting body only cultivated in the solid media of 100%PR, 25%SSC and 25%DBSM (redefined as PR-SSC and PR-DBSM, respectively). The best cordycepin productivity from the fruiting body indicated its order for C. militaris cultivated in the media PR-DBSM. The yield of adenosine, cordycepic acid and polysaccharides of the fruiting body showed its order for C. militaris cultivated in the media PR-SSC. This indicated that the addition of SSC and DBSM could improve the productivities of cordycepin and adenosine from the fruiting body of C. militaris, but just only the addition of SSC could improve the yields of cordycepic acid and polysaccharides of the fruiting body. However, the formation of the fruiting body was totally inhibited when the addition of SSC or DBSM was more than 25%. The highest yields of cordycepin, adenosine and cordycepic acid from the solid media indicated that C. militaris mycelia cultivated in the media 50%DBSM, 50%SSC and 100%PR, respectively. The yields of polysaccharides from the solid media showed that there was no significant difference for C. militaris mycelia cultivated in all the solid media except for 100%DBSM with the less yield effect.

目錄      
中文摘要...................................................i
英文摘要..................................................ii
致謝.....................................................iii
目錄......................................................iv
表目錄...................................................vii
圖目錄..................................................viii
第一章 緒論...............................................1
1.1實驗動機................................................1
1.2研究內容................................................3
第二章 文獻探討...........................................4
2.1北蟲草簡介..............................................4
2.1.1北蟲草之成分......................................6
2.1.2蟲草素............................................8
2.1.3蟲草酸............................................9
2.1.4腺苷.............................................10
2.1.5蟲草多醣.........................................10
2.2紫米簡介...............................................11
2.2.1紫米之營養成分及功能性成分.......................11
2.3芝麻簡介...............................................14
2.3.1芝麻之營養成分及功能性成分.......................15
2.3.2芝麻粕...........................................16
2.4醬油...................................................18
2.4.1醬粕.............................................19
第三章 研究內容與方法....................................21
3.1 實驗材料與設備........................................21
3.1.1 實驗材料........................................21
3.1.2 實驗藥品........................................21
3.1.3 實驗設備........................................22
3.2實驗方法...............................................23
3.3 蟲草素及腺苷測定......................................27
3.4蟲草酸之測定...........................................29
3.5蟲草多醣測定...........................................31
第四章 結果與討論........................................33
4.1 子實體生長情形........................................33
4.2 蟲草素之測定..........................................34
4.2.1子實體之蟲草素含量...............................34
4.2.2底座之蟲草素含量.................................34
4.3 腺苷之測定............................................38
4.3.1子實體之腺苷含量.................................38
4.3.2底座之腺苷含量...................................38
4.4 蟲草酸之測定..........................................42
4.4.1子實體之蟲草酸含量...............................42
4.4.2底座之蟲草酸含量.................................42
4.5蟲草多醣之測定.........................................46
4.5.1子實體之蟲草多醣含量.............................46
4.5.2底座之蟲草多醣含量...............................46
第五章結論................................................50
參考文獻..................................................51
附錄......................................................57
EXTENDED ABSTRACT.........................................65




參考文獻
[1]柴建萍、白興榮、謝道燕,2003,蛹蟲草主要有效成分及其藥理功效。雲南製藥科技,4:22-23。
[2]孫稅迎及張旭東,2002,冬蟲夏草與蛹蟲草特性分析。中醫藥學報,30(2):43-44。
[3]都興范、李業傑、王林華、石理鑫、徐宏、張俊濤、王曉燕、王鶴,2003,北冬蟲夏草的研究發展現況。通寧農業科學,4:26-28。
[4]王國棟,1995,冬蟲夏草類生態、培植、應用。科學文獻出版社,北京。
[5]許瑞祥,1984,人工培養冬蟲夏草屬之性質及其代謝物之探討。國立台灣大學農業化學研究所碩士論文。
[6]孫洪斌、曹晶、周宏璐、王克俠,2010,蛹蟲草與冬蟲夏草的同功性分析。農產品加工.創新版,第七期。
[7]朱雅紅、桂仲爭,2009,蛹蟲草液體菌種通氣發酵培養及其營養成分分析。食品與生物技術學報,28(5):699-704。
[8]Cunningham, K. G., Manson, W., Spring, F.S. and Hutchinson, S. A., 1950. Cordycepin, a metabolic product isolated form cultures of Cordyceps militaris (Linn.) Link. Nature, 166, 949-951.
[9]Gonzalez-Scarano F., Baltuch, G., 1999. Microglia as mediators of inflammatory and degenerative diseases. Annu Rev Neurosci, 22,219-240.
[10]Kuznetsov, D. A., and Musajev, N. I., 1990. The molecular mode of brain Mrna process damage followed by the suppression of post-transcriptional poly (A) synthesis with cordycepin. International Journa of Neuroscience, 51(1-2):53-67.
[11]難波恆雄,2004,神奇蟲草淨化血液克服現代病,中國醫藥大學出版社。
[12]陳登科,2004,超臨界二氧化碳萃取蛹蟲草蟲草素之探討。朝陽科技大學應用化學系研究所碩士論文。
[13]Chatterjee R, Srinivasan KS, Maiti PC., 1957. Cordyceps sinensis : structure of cordycepic acid. J Am Pharm Assoc. 46:114.
[14]Sprecher M, Sprinson DB., 1963. A reinvestigation of the “ Cordycepic acid”. J Org Chem. 28(9):2490.
[15]馬冰如、何玲、張甲生、古恒生、苑貴華、羅曼逸、李井洛、陳丹,1994,蠶蛹蟲草與冬蟲夏草化學成分的比較,中國食用菌,13(1):34-37。
[16]韓大夫,2001,冬蟲夏草健康奇蹟,成陽出版社。
[17]Chang, H. L., Chao, G. R., Chen, C. C., Mau, J. L., 2001. Non-volatile taste components of Agaricus blazei, Antrodia camphorata and Cordyceps militaris mycelia. Food Chemistry, 74, 203-207.
[18]S. P. Yang, F. Q. Tsim, K. W. K., 2006. Quality control of Cordyceps sinensis, a valued traditional Chinese medicine. J. Pharm. Biomed. Anal, 41, 1571-1584.
[19]李曉明、戴如琴、朱勤,1989,蟲草發酵液中多糖研究,中國中藥雜誌14(2):31-33。
[20]潘子明,2003,機能性發酵製品-低聚醣的真菌多醣。生技產業Bioindustry Vol.14 NO.2。
[21]王菊風,2006,蛹蟲草培養及其生理活性物質研究。中南林業科技大學碩士論文。
[22]Juliano, B. O., and Bechtel, D. B., 1985. The rice grain and its gross composition. p.17-19. in : Rice Chemistry and Technology. (Juliano, B. O. eds.) The American Association of Cereal Chemists, Inc. St. Paul, Minnesota, USA.
[23]Sugnano, M., Akimoto, K., 1993. sesamin: A multifunctional gift from nature. The Journal of clinical Nutrition Society, pp. 1-11.
[24]Akimoto, K., Winske, D., Gary, S.P. and Thomsen, M.F., 1993. Nonlinear evolution of electromagnetic ion beam instabilities. Journal of Geophysical Research, pp. 148-227.
[25]Hasegawa, K.; Murata, M.; Fujino, S., 1978. Agric. Biol. Chem. 42, 2291-2297.
[26]曾志正,2001,科學發展月刊。第29卷第9期,653-657。
[27]Kamal-Eldin A., Pettersson D., Appelqvist LA., 1995. Sesamin(a compound from sesame oil) increases tocopherol levels in rats fed ad libitum, Lipids, pp. 499-505.
[28]楊心豪,手工釀造醬油,台灣傳產,60-67。
[29]王國棟、晉天春,1994,冬蟲夏草對免疫功能的影響。中成藥16(11): 43-44。
[30]王西華、林玉娟、程梅萍,1994,固態發酵的回顧與展望。生物產業,5(3): 17-24。
[31]王蕾、于榮敏、張輝、賓文、汪文、李春燕、吳海燕,2003,人工培養蛹蟲草多醣的分離純化及其結構的初步研究。中國生化藥物雜誌,24(2): 23-25。
[32]代君君、范濤、吳傳華、肖林珍、田善富,2007,人工栽培蛹蟲草研究的概述。安徽農業科學,35(18):5469-5471。
[33]史敏、趙宇、溫學森,2009,人工培養北蟲草子座和培養基中多醣和核苷類成份的含量分析。食品與藥品,11:45-47。
[34]甘賓賓、潘艷坤,2006,HPLC法測定保健食品中蟲草素和腺苷含量。中國衛生檢驗雜誌,16(11):1310-1311。
[35]任士升,1998,人工培育蛹蟲草優質高產的技術環節。中國食用菌,17(1): 22-23。
[36]李翠新、張國慶、何永珍、王賀祥,2007,野生蛹蟲草的分離與高產菌株的篩選。中國食用菌,26(2):20-21。
[37]周佳賢,2005,蛹蟲草固態培養研究探討。朝陽科技大學生物技術研究所碩士論文。
[38]林群英、宋斌、鐘月金、李泰輝,2006,蛹蟲草人工栽培條件優化研究。中國食用菌,25(6):17-19。
[39]桂仲爭、朱雅紅,2008,蛹蟲草的人工培育、有效成分及藥理作用研究進展。蠶業科學,34(1):178-184。
[40]張飛翔、張慶,2001,蛹蟲草人工瓶栽技術要點。中國食用菌,20(4): 29-30。
[41]貢成良、吳丕東、徐承東、楊昆、陳國剛,2002,家蠶蛹蟲草的化學成份分析。蠶藥科學,28(2): 168-172。
[42]楊盛行,2002,固態發酵在農工業上之應用。科學農業。50(1-2):156-167。
[43]Agutter, P.S. and Mccaldin, B., 1979. Inhibition of ribonucleic acidefflux from isolate SV-40-3T3 cell nuclei by 3’-deoxyadenosine(cordycepin). The Biochemical Journal, 180: 371-378.
[44]Beach L.R., 1978. Cordycepin-induction of newly synthesizedglobin messenger RNA. Journal of Biological Inorganic Chemistry, 253(8):2628-2632.
[45]Deitch, A.D. and Sawicki, S. G., 1979. Effects of cordycepin onmicrotubules of cultured mammalian cells. Experimental CellResearch, 118: 1-13.
[46]Glazer, A.I. and Kuo, J. F., 1977. Inhibitory effects of codycepin oncyclic nucleotide-dependent and cyclic nucleotide-independentprotein kinases. Biochemical Pharmacology, 26: 1287-1290.
[47]Hesseltine, C. W., 1972. Biotechnology report: solid state fermentations. Biotechnology and Bioengineering, 14: 517-532.
[48]Latorre, J. and Perr, R.P., 1974. Relationship between polyadenylated heterogenous nucleic RNA and m-RNA studies with actinomycin-Dand cordycepin. Biochimica et Biophysica Acta, 335(1): 93-95.
[49]Legraverend M., Glazer R.I., 1978. Inhibition of phosphorylation of non-histone chromosomal protein by cordycepin and xylosylaminein L1210-cell in vitro. Molecular Pharmacology, 14(6): 1130-1135.
[50]Li, S. P., Yang, F. Q., & Tsim Karl, W. K., 2006. Quality control of Cordyceps sinensis, a valued traditional Chinese medicine. Journal of Pharmaceutical and Biomedinal Analysis, 41, 1571-1584.
[51]Rosowsky, A., Lazarurs, H., Yamashita, A., 1976. Nucleosides 1,9-( 3’-alkyl-3’-alkyl-3’-deoxy-β-D-ribofuranosyl ) adeninebiological studies. Journal of Medicinal Chemistry, 19(11):1265-1279.
[52]Wang, Q., 2002. Comparing study on the effect of cleaning hydroxyl radical between natural Cordyceps militaris and cultured Cordyceps militaris. Journal of Liaoning Teachers College, 4(3), 43-45.


QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔