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研究生:劉曉儒
研究生(外文):Liu, Hsiaoju
論文名稱:化學計量輔助開發固相微萃取裝置結合氣相層析質譜術偵測活體睡蓮花之揮發性成分
論文名稱(外文):Chemometric-assisted Developing SPME Combined with GC-MS Detection for Volatile Components of Living Water Lily
指導教授:蔡鳳婕
指導教授(外文):Tsai, Fengjie
口試委員:李茂榮吳錦生李名鏞張乃方
口試委員(外文):Lee, MawrongWu, ChinshengLee, MingyungChang, Naifang
口試日期:2013-07-24
學位類別:碩士
校院名稱:靜宜大學
系所名稱:化粧品科學系
學門:民生學門
學類:美容學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:92
中文關鍵詞:正交實驗設計法固相微萃取法睡蓮揮發性成分氣相層析質譜術
外文關鍵詞:Orthogonal experimental designSolid phase microextractionWater lilyVolatile compoundsGas chromatography–mass spectrometry
相關次數:
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睡蓮花香清幽宜人,但在大氣中濃度低,所以若想偵測其花香組成必須將氣味濃縮才可進行分離偵測。植物體一經離枝後香味可能會改變,為了測得真實花香同時必須將香味濃縮及顧慮環保概念,考慮擬以廢棄PE瓶設計成萃取腔室;而以固相微萃取法(solid phase microextraction, SPME)為萃取主體,硬體設計上SPME纖維放置位置,風扇及樣品之相關位置有可能影響結果的變因,變因以正交實驗設計結合統計分析方法以氣相層析質譜術(gas chromatography-mass spectrometry, GC-MS)偵測,定出裝置最佳組合而應用在活體睡蓮花之揮發性成分萃取分析。
研究中固相微萃取吸附纖維之選擇,根據層析峰個數和峰訊號強度,萃取睡蓮花之揮發性有機化合物以65 μm PDMS/ DVB纖維種類優於100 μm PDMS纖維。在不同花開程度下,以SPME之65 μm PDMS/ DVB纖維材質結合萃取裝置,在睡蓮花盛開初期,萃取到較多的揮發性有機化合物,氣相層析質譜儀之分析後,能鑑別出33個化合物。睡蓮的主要組成為乙酸苄酯(benzyl acetate)10.42 %、正十五烷(pentadecane)15.49 %、6,9-十七碳二烯(6,9-heptadecadiene)40.10 %以及8-十七碳烯(8-heptadecene)15.27 %。

Water lily is an aquatic plant belonging to the family Nymphaeaceae and genus Nymphaea, it has an elegant and pleasant aroma. Plant floral was low concentration in the atmosphere therefore the analysis of flower volatiles requires special methods for their isolation with enrichment. But the aroma of the flowers perceptibly changed following picking of the plant. For this purpose, an environmentally friendly and convenient plastic sampling chamber combine with solid phase microextraction (SPME) was designed, and this device parameters were studied by orthogonal experimental design to optimize.
According to the number of chromatographic peaks and peak signal strength, the PDMS/ DVB fiber fiber was found to be more efficient than 100 μm PDMS for applied to sampling of biogenic volatile compounds emitted from fresh flowers of water lily. Using the device combined with 65 μm PDMS/ DVB to monitor water lily bloom at different levels. The volatile compounds released from live flower of water lily were separated and identified 33 compounds by gas chromatography–mass spectrometry (GC-MS). The main components of the water lily were benzyl acetate (10.42 %),pentadecane (15.49 %),6,9-heptadecadiene (40.10 %) and 8-heptadecene (15.27 %).
摘要 Ι
Abstract Ⅲ
目錄 Ⅴ
圖目錄 Ⅷ
表目錄 Ⅹ

壹、緒論 1
一、前言 1
二、睡蓮簡介 4
三、生物源揮發性有機化合物萃取方法 6
四、固相微萃取法(Solid phase microextraction, SPME ) 13
五、正交實驗設計法(Orthogonal experimental design) 28
六、統計與分析 35
(一)直觀分析法(Intiutive analysis method) 36
(二)變異數分析(Analysis of variance, ANOVA) 37
七、分析 40
(一)定性-氣相層析質譜術 40
(二)定量-歸一化法(The Normalization Method) 48
八、研究目的 49
貳、實驗 50
一、材料與試劑 50
二、儀器設備 52
三、實驗步驟 54
四、加速固相微萃取法萃取裝置 56
五、數據統計與分析 60
參、結果與討論 61
一、加速固相微萃取法裝置最佳化之探討 61
(一) 裝置最佳化之探討Ⅰ 61
1. 直觀分析 61
2. 變異數分析 63
(二) 裝置最佳化之探討Ⅱ 66
1. 直觀分析 66
2. 變異數分析 69
二、 萃取纖維選擇 72
三、 裝置之效能證明 74
四、睡蓮揮發性成分組成 76
五、探討一天中睡蓮花之揮發性成分變化 79
肆、結論 82
伍、參考文獻 83

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