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論文名稱(外文):Application of Laccase Enzyme in Membrane Reactors for Removal of Emerging Contaminants in Water
指導教授(外文):Sung, Menghau
外文關鍵詞:LaccaseIbuprofenNaproxen2,4-DimethylphenolDialysis membraneHollow fiberHollow fiber, Membrane reactor
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漆氧化酶 (Laccase)有極高的氧化還原能力相較於其他種類之酵素,且反應之產物為水,不會造成環境二次之污染,因此本研究利用Laccase固定於薄膜反應器去除水中的新興污染物的布洛芬 (IBU)、萘普生 (NPX)和2,4-二甲基酚 (2,4-DMP)。本研究選擇的薄膜反應器為生物性之透析膜 (DMR)及中空纖維管膜 (HFR)作為反應器,探討添加不同劑量的Laccase固定於兩者反應器在不同pH值之降解污染物質的差異性。兩者反應器降解污染物質差異性之實驗結果顯示,Laccase於DMR的去除效果有限,相較於HFR有較佳之處理效果。Laccase不同劑量之實驗結果,高濃度的Laccase,能夠增加Laccase的活性,亦會提升處理效率。本研究之結果可應用於未來在處理廢水中的新興污染物,是一種具有發展潛力的技術。
Laccase has a very high redox potential compared to other enzymes. In this study, two types of membrane reactors, including dialysis membrane reactor (DMR) and hollow fiber reactor (HFR), were selected as Laccase immobilization tools to treat emerging contaminants such as ibuprofen, naproxen, and 2,4-dimethylphenol in water. Two controlling factors investigated are Laccase dosage and solution pH. Results indicate that HFR has a better performance than DMR. Also, the treatment efficiency increases as the Laccase dosage increases. These data suggest that HFR has the potential to be used in large scale in the future as a chemical-free treatment method in removing emerging contaminants.
第一章 前言 1
1-1新興污染物之定義和藥物及個人保健用品之分類 1
1-2非類固醇消炎藥 (NSAID)之來源與特性及對環境生態之影響 2
1-3 NSAID常見之處理方法 4
1-4漆氧化酵素 ( Laccase )之特性與實際應用 5
1-4-1酵素活性 5
1-4-2 Laccase和Laccase-介質系統之反應機制 6
1-5 酵素固定於薄膜反應器 7
1-5-1 透析膜之介紹 9
1-5-2中空纖維管柱之介紹 10
1-6 研究目的 12
2-1實驗架構 13
2-2實驗材料配製 14
2-2-1 實驗用純水 14
2-2-2緩衝溶液配製 14
2-2-2配製酵素溶液 15
2-2-3 ABTS溶液配製 15
2-2-4 污染物質之標準水溶液與檢量線之配製 15
2-3酵素活性測定 17
2-3-1 pH值對Laccase活性之影響 18
2-3-2 溫度對Laccase活性之影響 18
2-4 透析實驗 20
2-5 酵素直接與污染物質反應 22
2-6透析膜反應器 (DMR)之吸附實驗 23
2-7 Laccase固定於透析膜反應器 (DMR)之降解實驗 24
2-8 中空纖維管柱反應器 (HFR)之吸附實驗 26
2-9酵素固定於中空纖維管柱反應器之降解實驗 27
2-10分析方法 30
第三章 結果與討論 31
3-1酵素活性測定 31
3-2 透析實驗 35
3-3 酵素直接與污染物質反應 37
3-4 透析膜反應器之吸附實驗 39
3-5 透析膜反應器之降解實驗 41
3-6 中空纖維管柱反應器之吸附實驗 49
3-7 中空纖維管柱反應器之降解實驗 52
3-8 直接反應與固定於反應器中降解效率之比較 57
第四章  結論與建議 59
參考文獻 62

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