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研究生:鄭金源
研究生(外文):Chin-Yuan Cheng
論文名稱:設計與製作微流道整合DEP-QCM分離精蟲
論文名稱(外文):DESIGN AND FABRICATION MICROFLUIDIC INTEGRATED DEP-QCM FOR SEPARATING X-SPERM AND Y-SPERM
指導教授:簡昭珩
指導教授(外文):Chao-Heng Chen
口試委員:簡昭珩
口試委員(外文):Chao-Heng Chen
口試日期:2013-07-26
學位類別:碩士
校院名稱:大同大學
系所名稱:機械工程學系(所)
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:98
中文關鍵詞:介電泳石英晶體微天秤微流道精蟲
外文關鍵詞:DEPQCMMicrofluidicsperm
相關次數:
  • 被引用被引用:0
  • 點閱點閱:245
  • 評分評分:
  • 下載下載:2
  • 收藏至我的研究室書目清單書目收藏:0
近十幾年來,由於微機電的技術越發成熟,其應用層面也越來越廣泛,如光學結構、感測類元件、生物晶片等,在各方面領域皆有出色的發展。隨者科技的進步,人們對於醫療品質的要求也日益提高,為了因應人體各種疾病的治療與檢測,微機電應用於生物晶片的技術被更多的人所注目、關切,並積極尋求突破的可能性。
  本論文中,針對人類在遺傳學中對於性別的不同需求,設計出以微流道晶片中的結構設計、環境成分的控制,對精蟲樣本在微流道的行動進行影響以求達到定性分離的效果。並使用DEP-QCM的設計,憑藉定量抓取的功能,驗證並得知此一生物晶片對精蟲的分離笑果與實際數據,以便未來的延伸與發展。
  在定性分離方面,本論文依據精蟲的酸鹼喜好,設計灣道結構的微流道並加以整合,使精蟲在微流道前進的過程中自然的因為自身特性而分成不同的兩個群體。在定量檢測方面,延伸並改良了往年陳勇州學長研發的DEP-QCM之介電泳設計,以特定的操控參數設定電壓、頻率、溶液導電度,使精蟲能順利的被吸附在金屬電極上,並量測分離的效果與數據。
In the recent year, MEMS technology have been developed and applied in various kinds of fields, such as optics, power, sensor, bioengineer and etc. However, with the improvement of quality of the life, people want to have better medical treatment. MEMS technology is applied by biotechnology such as separate bio particles to do some things like medical treatment.
In this paper, we focus on human genetics to choose different gender for every people requirement. We designed a microchip to affect sperm in microfluidic by the structure、PH value. After separation, we used DEP-QCM structure to detect and measurement all off the experiment controlled value and the QCM frequency value with voltage and frequency from function generator.
In this research, we design a corner and changed the PH value to affect the sperm between X-sperm and Y-sperm. We finally made much of the Y-sperm to turn direct by the different PH value with the buffer solution. And then, sperm would been adsorbed on the QCM and detected with frequency to proof the design is true and effective.
中文摘要 5
英文摘要 6
目錄 7
圖目錄 10
表目錄 16
第一章 簡介 17
1.1 前言 17
  1.2 研究動機 17
  1.3 研究目標 18
  1.4 研究架構 19
第二章 文獻回顧 20
  2.1 微流道文獻 20
  2.2 精蟲特性文獻 23
  2.3 介電泳文獻 24
  2.4 石英晶體微天平文獻 34
第三章 設計原理 38
  3.1 設計動機 38
  3.2 設計概念 39
    3.2.1 精蟲對酸鹼值的依賴性 39
    3.2.2 微流道彎取交會的設計 41
    3.2.3 石英晶體微天平的設計 42
3.2.4 分析與模擬DEP-QCM電場效應 43
  3.3 晶片系統整合 46
第四章 實驗製作 48
  4.1 實驗前處理 48
    4.1.1 建立微流道結構 48
    4.1.2 流道結構翻膜 52
4.1.3 流道防漏液測試 54
4.1.4 適當裁切並精簡化 56
  4.2 DEP-QCM檢測晶片 57
4.2.1 建立流道結構 57
4.2.2 定義上電極位置同時翻膜流道結構 60
4.2.3 定義QCM的架設位置 62
4.2.4 流道防漏液測試 63
4.2.5 適當裁切並精簡化 64
  4.3 雙晶片整合架設 64
4.3.1 通道橋梁架設 64
第五章 實驗量測 66
  5.1 量測系統架構 66
5.1.1 量測架構概念 66
5.1.2 實驗儀器介紹 67
5.1.3 實驗儀器規格 68
5.2 樣本製備 69
5.2.1 精蟲採集注意事項 71
5.2.2 精蟲溶液觀測確認 72
5.2.3 精蟲Buffer溶液調配 74
5.3 量測過程與結果 75
5.3.1 量測系統架設與量測步驟 75
5.3.2 頻率變化數據統計與趨勢 76
5.3.3 QCM的頻率變化與質量變化換算 87
5.4 進階實驗與結論 89
第六章 結論與未來工作 94
  6.1 結論 94
  6.2 未來工作 95
參考文獻 96
[1] Cheng-Hsin Chuang, Ching-Hua Wei "Depth Effects of DEP Chip with Microcavities Array on Impedance Measurement for Live and Dead Cells" Nano/Micro Engineered and Molecular System, 2008, P858-P864.
[2] Hui-Ting Fu, Da-Jeng Yao "Laminar Stream-Based Microfludic Chip with High Efficiency for Human Sperm Motility Sorting" Nano/Molecular Medicine and Engineering, 2010, p72-p76.
[3] Yahya Hosseini, Phillip Zellner, Masoud Agah "A Single-Mask Process for 3-D Microstructure Fabrication in PDMS" MICROELECTROMECHAMICAL SYSTEMS, 2013, p356-p363.
[4] Sigal Peer, Fina Eltes, Arie Berkovitz "Is Fine Morphology of the Human Sperm Nuclei Affected by in Vitro Incubation at 37℃?" Fertility and Sterility, Vol 88,2007, p1589-p1594.
[5] T. Qiu, C. Han, R. Ma, L. Xie, Z. Li, K. Su "A Microfluidic Treatmill for Sperm Selective Trapping According to Motility Classification" Conference on Transducers, 2011 IEEE, p1320-p1324.
[6] Pohl, H.A. "Some Effects of Nonuniform Fields on Dielectrics" J. Appl. Phys, 1958, p-1182-p1188.
[7] Pohl, H.A. "Dielectrophoresis" Cambridge University Press, 1978, p328-p334.
[8] S. Masuda, M. Washizu, I. Kawabata "Movement of Blood Cells in Liquid by Nonuniform Traveling Field" IEEE Trans, IAS, 1988, p217-p222.
[9] Y. Huang, X.B. Wang, J.A. Tame "Electrokinetic Behavior of Colloidal Particles in Travelling Electric Fields : Studies Using Yeast Cells" Journal of Physics, 1993, p1528-p1535.
[10] M.P. Huges, R. Pething, X.B. Wang "Dielectrophoretic Forces on Particles in Travelling Electric Fields" Journal of Physics, 1995, p474-p482.
[11] P. Gascoyne, Y. Huang, M.P. Hughes "Manipulation of Erythroleukemia Cells Using Traveling Electric Fields" Proc. IEEE, 1994, p772-p775.
[12] Frederick. F, Xiao-bo Wang "Separation of Human Breast Cancer Cells from Blood by Differential Dielectric Affinify" Proc. Natl. Acad, 1955, p92-p98.
[13] Hywel Morgan, Michael P. Hughes "Separation of Submicron Bioparticles by Dielectrophoresis" Biophysical Journal, 1999, p516-p525.
[14] Suzuki Masato, Yasukawa Tomoyoki "Separation of Live and Dead Micro-organisms in a Micro-Fludic Device by Dielectrophoresis" 2002.
[15] Haibo Li, Rashid Bashir "Dielectrophoresis Separation and Manipulation of Live and Heat-Treated Cells of Listeria on Microfabricated Device with Interdigitated Electrodes" Sensors and Actuator, 2002, p215-221.
[16] 曾光明 "生物微粒之分離與操控晶片" 台北國立科技大學機電整合研究所,2006.
[17] Matthew S. Pommer, Yanting Zhang "Dielectrophoretic Separation of Particles from Diluted Whole Blood in Microfludic Channels" Electrophoresis, 2008, p1213-p1218.
[18] Luke A. Macqueen, Michael D. Buschmann "Gene Delivery by Electroporation after Dielectrophoretic Positioning of Cells in a Non-Uniform Electric Fields"Bioelectrochemistry , 2008, p141-p148.
[19] Stefan Kostner, Sander Van, Wojciech Witarski "Guided Dielectrophoresis : A Robust Method for Continuous Particle and Cell Separation" IEEE Sensor Journal, 2010, p1440-p1447.
[20] Khashayar Khoshmanesh, Sara Baratchi "On-Chip Separation of Lactobacillus Bacteria from Yeasts Using Dielectrophoresis" Microfludic and Nanofludic, 2012, p597-p606.
[21] Sauerbrey-Z. Phys. , 1959. p206-210.
[22] Sauerbrey-Z. Phys. , "Use of Quartz Vibrator for Weighing thin Films on a Microbalance" 1959, p364-p371.
[23] DE Elliott, WH Elliott, KM Jones "Data for Biochemical" 1969, p593-p608.

[24] Rongzhang Hao, Dianbing Wang, "Rapid Detection of Bacillus Anthracis Using Monoclonal Antibody Functionalized QCM Sensor" Bio Sensor and Bio Electric, 2009, p1330-p1335.
[25] Henry O. Fatoyinbo, Kai F, Hoettges, "An Integrated Dielectrophoretic Quartz Crystal Microbalance (DEP-QCM) Device for Rapid Biosensing Applications"
Bio Sensor and Bio Electrertics, 2007, p225-p232.
[26] Takashi Abe, Makoto Higuchi "A Monolithic QCM Array Designed for Mounting on a Flow Cell" IEEE Sensor Journal, 2011, p86-p91.
[27] http://www.mmh.org.tw/MackayInfo2/article/B310/647.htm
[28] http://www.e-stork.com.tw/viewArticle.do.id=821
[29] Shettles L.B. "After Office Hours : Conception and Birth Sex Ratios" Obstet Gynec, 1961, p122-p130.
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