臺灣博碩士論文加值系統

Colorectal cancer is the leading cause of cancer mortality, and metastasis is responsible for approximately 40 % of death in colon cancer patients. Hence, the effective inhibition on metastasis should prolong the life of colon cancer patients. Epithelial-mesenchymal transition (EMT) is an essential step for metastasizing and several adhesion proteins and chemokines are associated to the step. The regulation of adhesion proteins and chemokine are supposed to be able to inhibit cell metastasis. Sinapinic acid (3,5-dimethoxy-4-hydroxycinnamic acid, SA) and m-coumaric acid (3-hydroxycinnamic acid, m-CA) are cinnamic acid analogues that widely exist in a variety of plant foods. A variety of physiological activities, such as the inhibition of tumor necrosis factor, anti-inflammation, anti-oxidation, and anti-allergy, have been found in SA and m-CA. However, the effect of SA and m-CA on inhibiting cancer cell invasion is still unclear. The purpose of this study was to investigate the effect and mechanism of SA and m-CA on the inhibition of colorectal cancer cells invasion. The colorectal cancer cells, Colo205 and HT29, were treated with SA and m-CA at a nontoxic dose (0, 10, 50, 100, 150 M), and the cell-matrix adhesion and EMT status were determined. Western blotting was employed to analyze the levels of adhesion-related proteins and chemokines. Moreover, the inhibitory activities on invasion and tube formation of SA and m-CA were also assayed. The results showed that SA and m-CA significantly inhibited cell-matrix adhesion and EMT of Colo205 and HT29 cells at a concentration of  100 M; the increase of E- and P-cadherin and decrease of N-cadherin, vimentin, CXCR2, CXCR4, and CXCL8 in Colo205 and HT29 cells by treating with SA and m-CA were detectable; the cell invasion and tube formation inhibited by SA and m-CA were further verified. In conclusion, SA and m-CA might be the natural bioactives that having the capability to inhibit invasion of colorectal cancer cell. The anti-invasion effect of SA and m-CA was through inhibiting cell adhesion activity via increasing E- and P-cadherin and decreasing N-cadherin, vimentin, CXCR2, CXCR4, and CXCL8 as well as suppressing the angiogenesis.

目錄………………………………………………………………………I
圖目錄…………………………………………………………….……III
表目錄………………………………………………………………….VI
中文摘要…………………………………………………………………1
Abstract………………………………………………………………3
第一章、 前言...…………………………………………………………5
第二章、文獻整理…………………………………………………..……6
壹、癌症………………………………………………………..…………6
貳、大腸直腸癌之簡介…………………………………………..………6
參、腫瘤轉移與侵入…………………………………………….……...12
肆、上皮-間質細胞轉型 (epithelial-mesenchymal transition, EMT)
與黏附蛋白、趨化因子之關係……………...................................29
伍、天然多酚類化合物……………………………………………...….37
陸、研究目的………………………………………………………...….42
柒、研究架構………………………………………………………...….43
第三章、材料與方法………………………………………...………….44
第四章、結果………………………………………………...………….54
一、白芥子酸及香豆酸對人類大腸直腸癌細胞 Colo205 與
HT29 細胞存活率之影響…………………………….…....... 54
二、白芥子酸及香豆酸對大腸直腸癌細胞 MMP-9 與 MMP-2
活性之影響……………………..…………………………...…54
三、白芥子酸及香豆酸對大腸直腸癌細胞 uPA 活性之影響....55
四、白芥子酸及香豆酸對大腸直腸癌細胞-間質黏附能力之影響...55
五、白芥子酸及香豆酸對人類大腸直腸癌細胞抑制 EMT 分析..56
六、白芥子酸及香豆酸對 -catenin, snail, 1-integrin 蛋白
表現量之分析………………………………...……….……….56
七、白芥子酸及香豆酸對鈣黏蛋白 E-cadherin 與 P-cadherin
蛋白表現量之分析……………………………………….……57
八、白芥子酸及香豆酸對鈣黏蛋白 N-cadherin 與 vimentin
蛋白表現量之分析……………………………………………..57
九、白芥子酸及香豆酸對趨化因子 CXCR2, CXCR4, CXCL8
蛋白表現量之分析……….........................................................58
十、白芥子酸及香豆酸對大腸直腸癌細胞侵入能力之影響….....58
十一、白芥子酸及香豆酸對 HUVEC 細胞脈管成形之影響….....59
第五章、討論…………………………………………...……………….60
第六章、結論…………………...……………………………………….83
參考文獻……………………………………………………….……….84

台灣地區 2011 年主要死因統計 (2012)。2012年 08 月 16 日，
取自:行政院衛生署衛生統計資訊網，http://www.doh.gov.tw。
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