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研究生:莊心源
研究生(外文):Hsin-Yuan Chuang
論文名稱:發展架設於自製顯微鏡之微型化二維陣列取樣光譜儀
論文名稱(外文):Development of a compact bidimensional sampling spectrometer based on a home-made microscope
指導教授:陳浩夫
指導教授(外文):How-Foo Chen
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:生醫光電研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:英文
論文頁數:36
中文關鍵詞:取樣光譜儀二維陣列
外文關鍵詞:Sampling spectrometertwo dimensional microarray
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傅立葉轉換光譜儀是一項重要的檢測技術,它可提供準確的頻譜分析。標準的傅立葉轉換光譜儀在架構上使用移動式反射鏡設計,該設計會在檢測過程中產生震動而有可能影響精準度。因此為了改善此設計,特魯瓦科技大學的研究人員開發了二維陣列取樣光譜儀,這項技術使用了駐波整合式傅立葉光譜儀的原理使其在設計上不使用移動式反射鏡而在檢測過程中能保持靜態。
在之前的研發中,二維陣列取樣光譜儀已被證實能夠提供單一波長及多重波長的光譜測量,且其準確度已足夠描繪Fianium可調式雷射的頻譜;然而此方法在架設設計上必須使用Olympus顯微鏡來收集散射光,因此為了降低成本及架設微小化,我們發展了一套簡易的自製顯微鏡來解析光譜,且此套架設成本能小於1600歐元。在經過多次測試後,自製顯微鏡在解析單一波長光譜上能和Olympus顯微鏡有相同的解析力;然而在多重波長光源的狀況下,聚焦透鏡所產生的色差會影響解析光譜而產生雜訊。
雖然此套系統並不完美,但它代表了能將二維陣列取樣光譜儀的商用顯微鏡替換成自製顯微鏡的可能性。

Fourier transform spectroscopy (FTS) is an important measurement technology for providing. However, a typical Michelson type FTS includes a moving mirror design causing vibration during measurement. The vibration could affect the stability of device, so researchers in University of Technology of Troyes developed a compact two-dimensional sampling spectrometer (Cobiss) that is based on stationary-wave integrated Fourier transform spectrometry (SWIFT). This Cobiss system can measure a spectrum without moving parts, making the whole measurement a static process. In the previous works, Cobiss has been proven the feasibility of measuring spectrum in single and multiple wavelengths detection. The results are precious and accurate enough to depict the bandwidth of Fianium tunable laser. But for the collection of scattering light from Cobiss, a large and expensive commercial Olympus is used. In order to cost-down and shrink the whole device of Cobiss, we developed a simple home-made microscope to fit Cobiss and resolve spectra, and the whole cost is less than 1600 euro. In a series of testing, the home-made microscope can perform as well as the commercial microscope in resolving spectrum in single wavelength detections. In multiple wavelengths measurement, chromatic aberration caused by the focusing lens will affect the results.
Although the system is not perfect, it stands for a possibility of replacing the commercial microscope in Cobiss system to a home-made one.

Contents i
Chinese Abstract ii
Abstract iii
I. Introduction 4
II. Experimental and Theoretical method 5
2.1 Evanescent wave interference 5
2.2 Configuration of modified Cobiss system 8
2.3 Lift-off process and sample preparation 9
2.4 Home-made microscope set-up for Cobiss system 11
III. Results 12
3.1 Numerical simulation of evanescent field interference by Matlab program 12
3.2 Resolving spectrum in Cobiss by a commerical microscopy 16
3.3 Development and resolving power of Cobiss using a home-made microscope 23
IV. Conclusion 30
V. Perspectives 31
VI. Reference 33
VII.Appendix 34

1. Michael Bradley, Ph.D., Thermo Fisher Scientific, Madison, WI, USA. Advantages of a Fourier Transform Spectrometer. Thermo electron scientific instruments LLC.

2. Etienne Le Coarer, Sylvain Blaize, Pierre Benech, Ilan Stefanon, Alain Morand, Gilles Lerondel, Gregory Leblond, Pierre Kern, Jean Marc Fedeli and Pascal Royer. Wavelength-scale stationary-wave integrated Fourier-transform spectrometry
Nature photonics. Vol 1, August, 2007.

3. Mikael Renault, Yassine Hadjar,* Sylvain Blaize, Aurélien Bruyant, Laurent Arnaud,Gilles Lerondel, and Pascal Royer. Bidimensional near-field sampling spectrometry. OPTICS LETTERS., Vol. 35, No. 19, 3303. (2010)

4. M. Siler, T. Cizmar, M. Sery and P. Zemanek. Optical forces generated by evanescent standing waves and their usage for sub-micron particle delivery. Appl. Phys. B 84, 157–165 (2006)

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