(3.238.130.97) 您好!臺灣時間:2021/05/18 20:17
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

: 
twitterline
研究生:劉芷岑
研究生(外文):Jr-Tsen Liou
論文名稱:黑殭菌及黑殭菌素與小皮傘及其代謝物的培養觀察與初步探討
論文名稱(外文):Preliminary study on Cultivation and metabolites of Metarhizium anisopliae and Marasmius oreades
指導教授:曾耀銘曾耀銘引用關係
指導教授(外文):Yew-Min Tzeng
口試委員:張耀南劉炳嵐
口試委員(外文):Yew-Nan ChangBing-Lan Liu
口試日期:2013-07-31
學位類別:碩士
校院名稱:朝陽科技大學
系所名稱:應用化學系生化科技碩博士班
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:89
中文關鍵詞:黑殭菌黑殭菌素小皮傘
外文關鍵詞:MtarhiziumDestruxinsMarasmius
相關次數:
  • 被引用被引用:0
  • 點閱點閱:283
  • 評分評分:
  • 下載下載:4
  • 收藏至我的研究室書目清單書目收藏:0
真菌的二次代謝產物具有許多生理活性,如抗菌、抗癌、抗發炎、抗真菌。黑殭菌屬(Metarhizium)為昆蟲病原真菌的一種,廣泛使用於世界各地農業害蟲及疾病媒介控制方案中,可替代以往使用的化學殺蟲劑。事實上,環縮酚酸胜肽加上天然環胜肽,成為一個以上酯鍵連結為特色的天然產物大家族,此類天然產物,由於其具有多樣化生物活性,而引起科學界廣大興趣,包含抗癌、抗生素、抗真菌、免疫抑制劑或抗發炎。本實驗將以5L攪拌式發酵槽發酵培養黑殭菌素,並以乙酸乙酯萃取其成分,續以半製備型HPLC分離純化並大量收集,此外,研究顯示小皮傘二次代謝物可抑制孢子萌發,作為抗生素或生物殺蟲劑。本章將探討真菌小皮傘固態培養,並觀察其生長型態,了解小皮傘其他生理活性。
Secondary metabolites of fungi has many physiological activities such as antibacterial, anti-cancer, anti-inflammatory, anti-fungal. Matarhizium is a genus of entomopathogenic funfi used world-wide as alternatives to chemical insecticides in agricultural pest and disease-vector control programs, which can replace chemical pesticides used in the past. In fact, cyclodepsipeptide peptide plus natural peptide ring, becoming a more ester bonds linked featuring a large family of natural products, such natural products, due to their diverse biological activity, which cause scientist interested, because it include anticancer, antibiotic, antifungal, anti-inflammatory and immunosuppressive agents. The destruxin used in this study were isolated and purified from submerged culture,and extracted with ethyl acetate. And used semi-preparative HPLC purification and large collection.Furthermore, before studies show secondary metabolites of Marasmius can inhibit spore germination, it can be antibiotics or biocides. In this chapter we investigate the solid culture of fungus Marasmius , and to observe their growth patterns, understand Marasmius other physiological activity.
摘要 Ⅰ
ABSTRACT Ⅱ
致謝 Ⅲ
表目錄 IⅤ
圖目錄 V
第一章 緒論 1
1-1 前言 1
1-2 文獻回顧 2
第二章 黑殭菌素之分離純化 19
2-1材料與方法 19
2-2黑殭菌萃取 21
2-3黑殭菌素分離純化 22
2-4高壓液相層析儀 22
2-5結果與討論 22
第三章 小皮傘真菌研究探討 24
3-1前言 24
3-2文獻回顧 26
3-2-1硬柄小皮傘簡介 26
3-2-2小皮傘應用 32
3-2-4菇類含有其他活性物質 41
3-3材料與方法 49
3-3-1Marasmius sp.菌種保存 49
3-3-2 Marasmius sp.(A)菌平面培養 49
3-3-3 Marasmius oreades.(硬柄小皮傘)菌種保存 50
3-3-4 Marasmius oreades.(硬柄小皮傘)(B)平面培養 50
3-4結果與討論 52
3-5結論與展望 53
參考文獻 80

表目錄
表 1、黑殭菌素的共通結構和已知的黑殭菌素 15
表 2、黑殭菌素定性和定量分析方法總結 18


圖目錄
圖 1、黑殭菌之平面培養14天 54
圖 2、前培養之菌絲球型態 55
圖 3、主培養之菌絲球型態 56
圖 4、黑殭菌在五公升發酵槽培養第十四天 57
圖 5、發酵液萃取 58
圖 6、半製備型HPLC層析圖譜 59
圖 7、marasmal B、.marasmal C、.marasmene B、epi-3 60
圖 8、marasmic acid 61
圖 9、alliacol A、alliacol B 61
圖 10、MniopetalsA-F(1a-1f) 62
圖 11、Kuehneromycin A 、Kuehneromycin B 63
圖 12、Aspergillus 64
圖 13、illudin M、illudin S 65
圖 14、6-deoxyilludin M、6-deoxyilludin S 65
圖 15、merulidial 1-7 66
圖 16、Pilatin 67
圖 17、小皮傘Marasmius sp.平面培養Day1. 68
圖 18、小皮傘Marasmius sp.平面培養Day2. 68
圖 19、小皮傘Marasmius sp.平面培養Day6. 69
圖 20、小皮傘Marasmius sp.平面培養Day7. 69
圖 21、小皮傘Marasmius sp.平面培養Day8. 70
圖 22、小皮傘Marasmius sp.平面培養Day9. 70
圖 23、小皮傘Marasmius sp.平面培養Day10. 71
圖 24、小皮傘Marasmius sp.平面培養Day11. 71
圖 25、小皮傘Marasmius sp.平面培養Day12. 72
圖 26、小皮傘Marasmius sp.平面培養Day13. 72
圖 27、小皮傘Marasmius sp.平面培養Day14. 73
圖 28、硬柄小皮傘Marasmius oreades.平面培養Day3. 73
圖 29、硬柄小皮傘Marasmius oreades.平面培養Day4. 74
圖 30、硬柄小皮傘Marasmius oreades.平面培養Day5. 74
圖 31、硬柄小皮傘Marasmius oreades.平面培養Day6. 75
圖 32、硬柄小皮傘Marasmius oreades.平面培養Day7. 75
圖 33、硬柄小皮傘Marasmius oreades.平面培養Day8. 76
圖 34、硬柄小皮傘Marasmius oreades.平面培養Day9. 76
圖 35、硬柄小皮傘Marasmius oreades.平面培養Day10. 77
圖 36、硬柄小皮傘Marasmius oreades.平面培養Day11. 77
圖 37、硬柄小皮傘Marasmius oreades.平面培養Day12. 78
圖 38、硬柄小皮傘Marasmius oreades.平面培養Day13. 78
圖 39、硬柄小皮傘Marasmius oreades.平面培養Day14. 79

參考文獻
葉哲豪, 「黑殭菌素的回收與純化之探討」,碩士論文,朝陽科技大學應用化學研究所,台中 ( 2002 ) 。
黃玄舜, 「黑殭菌素分離純化製備與毛細管電泳分析方法之探討」,碩士論文,國立東華大學生物技術研究所,台中(2002)。
黎培琪,「黑殭菌素 ( Destruxin B ) 對 DBA/2 老鼠L5178Y淋巴癌細胞之抑制效果及其機制」,碩士論文,私立台北醫學大學生物醫學技術研究所,台中 (2004)。
陳拯裕,「 黑殭菌素B 對於人類大腸癌及肝癌細胞株之抗癌機制與探討 」,碩士論文,朝陽科技大學生物技術研究所。台中(2005)。
王盈涵,「 黑殭菌素B ( Destruxin B )、愛瑞莎 ( Iressa ) 合併使用對抗肺癌細胞之腫瘤機制探討」,碩士論文,臺北醫學大學醫學檢驗暨生物技術研究所,臺北 (2007)。
鄒嘉恒,「黑殭菌素 E 之發酵製備與回收純化」,碩士論文,朝陽科技大學 應用化學系,台中(2005)。
高穗生、蔡勇勝,「蟲生病源真菌在害蟲防治上之利用」,藥試所專題報導,(1995)38: 4-11。
高穗生、蔡勇勝「蟲生病源真菌在害蟲防治上之利用」,藥試所專題報導,(1996) 39:1-16。
張義璋,「水稻稻熱病防治手冊」,行政院農業委員會農業試驗所出版(2001)。
謝式坢鈺、梁文進, 「 稻熱病菌感染前之行為, 水稻病蟲害之生態學與流行學」 ,中國農村復興聯合委員會刊印,( 1978 )。
鄧富元、龔素芳、夏毅然、高文斌、謝耀東 「 NF-κB在發炎反應、細胞凋亡與癌症生成過程中的調控 」 Chin Dent J ,中華牙誌,( 2006 ) ; 25 ( 1 ) :12-24
水野草 川和正允,菇類的化學,生化學,(1977)。
周文能、張東柱,「野菇入門」, (2005)。
周文能、張東柱,「野菇圖鑑」,(2005)。

Abraham WR, Med. Bioactive sesquiterpenes produced by fungi: are they useful for humans as well. Chem. 2001;8: 583-606

Ayer WA, Craw PA, Stout TJ, Clardy J. Novel sesquiterpenoids from the fairy ring fungus Marasmius oreades.Can.J.Chem.Vol. 1989;733-778

Anke T, Watson WH, Giannetti BM, Steglich W.. Antibiotics from Basidiomycetes. XIII1 The Alliacols A and B from Marasmius alliaceus. 1981.

Aldridge, D. C., and W.B. Turner. 1969. "Structures of cytochalasin C and cytochalasin D from Metarhizium anisopliae." J Chem Soc C. 6: 923–928.

Amagata, T., B.I. Morinaka, A. Amagata, K. Tenney, F.A. Valeriote, and E. Lobkovsky. 2006. " A chemical study of three novel cyclic depsipeptides produced by a sponge-derived fungus " J Nat Prod no. 69:1560–5.

Cai, P., D. Smith, B. Katz, C. Pearce, D. Venables, and D. Houck. 1998. "Destruxin-A4 chlorohydrin, a novel destruxin from fungus OS-F68576: isolation, structure determination, and biological activity as an inducer of erythropoietin." J Nat Prod no. 61 (2):290-293.

Cavelier, F., R. Jacquier, J. L. Mercadier, J. Verducci, M. Traris, and A. Vey. 1997. "Destruxin analogs: variations of the alpha-hydroxy acid side chain." J Pept Res no. 50 (2):94-101.

Che, Y., D.C. Swenson, J.B. Gloer, B. Koster, and D. Malloch. 2001. "Pseudodestruxins A and B: new cyclic depsipeptides from the Coprophilous fungus Nigrosabulum globosum. ." J Nat Prod. 64:555–58.

Chang ST, Miles PG. Mushrooms: Cultivation,Nutritional Value, Medicinal Effect, and Environmental Impact. 2nd ed,CRC Press.Boca Raton. 2004; 27

Cho Y, Gorina S, Jeffrey PD, Pavletich NP. Crystal structure of a p53 tumor suppressor-DNA complex: understanding tumorigenic mutations. Science1994; 15(265): 346-355.

Denis J,Wrght. Nematicides: Mode of action and new approaches to chemical control. In:Plant parasitic nematicides) Vol II.I1981; p442-445.

Espada A, and Dreyfuss MM. 1997. "Effect of the cyclopeptolide 90-215 on the production of destruxins and helvolic acid by Metarhizium anisopliae. ." J Ind Microbiol Biotechnol no. 19:7-11.

Erkel G,Lorenzen K, Anke T, Velten R, Gimenez A,Steglich WZ. Naturforsch. 1995;50:1−10.

Flint DR, Chemical evidence for systemic nematicidal activity of NEMACUR Pflanzenschutz-Nachrichten Bayer .1977;30:153-163

Freudenthal R, Locatelli F, Hermitte G, Maldonado H, Lafourcade C, Delorenzi A, Romano A. Kappa-B like DNA-binding activity is enhanced after spaced training that induces long-term memory in the crab Chasmagnathus. Neurosci. Lett.1998;242 (3): 143–46

Gupta, S., S.B. Krasnoff, J.A.A Renwick, D.W. Roberts, J.R. Steiner, and J. Clardy. 1993. "Viridoxins A and B:novel toxins from the fungus Metarhizium flavoviride." J Org Chem no. 58:1062–7.

Heim J, Anke T, Mocek U, Steffan B, Steglich W. Antibiotics from basidiomycetes. XXIX: Pilatin, a new antibiotically active marasmane derivative from cultures of Flagelloscypha pilatii Agerer. J Antibiot 41: 1752–1757 (1988)

Halpern GM, Miller AH. Medicinal mushrooms. Ancient remedies for modern ailments. M. Evans and Company Inc., New York .2002;

Jauch, J. Synlett .1999; 1325.

Jauch, J. Angew. Chem. 2000, 112, 2874; Angew. Chem. Int.Ed. 2000, 2764

Jansen, de Groot.The synthesis of drimane sesquiterpenoids. 1991

Jansen, de Groot. Occurrence, biological activity and synthesis of drimane sesquiterpenoids. 2004.

Karin M, Cao Y, Greten FR, Li ZW. NF-jB in cancer:from innocent bystander to major culprit. Nat Rev Cancer .2002; 2:301–310

Kavanagh F, Hervey A, Robbins WJ. Antibiotic substances from basidiomycetes: IV. Marasmius conigenus. Proc Natl Acad Sci USA.1949; 35: 343-349


Kim, H.S., M. H. Jung, S. Ahn, C.W. Lee, S.N. Kim, and J.H. Ok. 2002. "Structure elucidation of a new cyclic hexadepsipeptide from Beauveria feline." J Antibiot no. 55:598–601.

Kondo, S., N. Meguriya, H. Mogi, T. Aota, K. Miura, T. Fujii, I. Hayashi, K. Makino, M. Yamamoto, and N. Nakajima. 1980. "K-582, a new peptide antibiotic. I." J Antibiot (Tokyo) no. 33 (6):533-42.

Krasnoff, S. B., C. H. Sommers, Y. S. Moon, B. G. Donzelli, J. D. Vandenberg, A. C. Churchill, and D. M. Gibson. 2006. "Production of mutagenic metabolites by Metarhizium anisopliae." J Agric Food Chem no. 54 (19):7083-8. doi: 10.1021/jf061405r.

Krasnoff, S.B., I. Keresztes, R.E. Gillilan, Szebenyi D.M.E., B.G.G. Donzelli, and A.C.L. Churchill. 2007. "Serinocyclins A and B, cyclic heptapeptides from Metarhizium anisopliae." J Nat Prod no. 70:1919–24.

Kuboki, H., T. Tsuchida, K. Wakazono, K. Isshiki, H. Kumagai, and T. Yoshioka. 1999. "Mer-f3, 12-hydroxy-ovalicin, produced by Metarrhizium sp. f3." J Antibiot (Tokyo) no. 52 (6):590-3.

Kuschel A, Anke T, Velten R, Klostermeyer D;Steglich, W.; König, The mniopwtals,new inhibitors of reverse transcriptases from Mnioptalum species (Basidiomycetes) I.Producing organism, fermentation , isolation and biological activitiesB.J. Antibiot. 1994, 47, 733.

Kuschel A, Anke T. The Mniopetals new inhibitors of reverse trascriptases from a Mniopetalum species (Basidiomycetes) I. Producing organism fermentation isolation and biological activities J. Antibiot.1994;47:733.

Liermann JC, Thines E, Opatz T, Anke H, Drimane Sesquiterpenoids from Marasmius sp Inhibiting the conidial germination of plant-pathogenic Fungi. Journal of Natural Products.2012; 75 (11):1983-1986.

Lira, S.P., A.M. Vita-Marques, M.H.R. Seleghim, T.S. Bugni, D.V. LaBarbera, and L.D. Sette. 2006. "New destruxins from the marine-derived fungus Beauveria feline. ." J Antibiot no. 59:553–63.

Levenson JM, Choi S, Lee SY, Cao YA, Ahn HJ, Worley KC,

Meffert MK, Chang JM, Wiltgen BJ, Fanselow MS, Baltimore D. NF-kappa B functions in synaptic signaling and behavior. Nat. Neurosci 2003;6 (10): 1072–8.

Manson MM, Holloway KA, Howells LM, Hudson EA, Plummer SM, Squires MS, Prigent SA . Modulation of signal transduction pathways by chemopreventive agents. Chemoprotection. 2000;28(2):7–12

March RW., Systemic fungicides. Longman, London and New York.1977

Merlo E, Freudenthal R, Romano A. The IkappaB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus. Neuroscience 2002;112 (1): 161–72.

Moon, Y.S., B.G.G. Donzelli, S.B. Krasnoff, H. McLane, M.H. Griggs, and P. Cook. 2008;. "Agrobacterium mediated disruption of a nonribosomal peptide synthetase gene in the invertebratepathogen Metarhizium anisopliae reveals a peptide spore factor. ." Appl Environ Microbiol no. 74:4366–80.

Morais, R.P., S.P. Lira, M.H.R. Seleghim, and R.G.S. Berlinck. 2010. "A method for destruxin analysis by HPLC-PDA-ELSD-MS." J Braz Chem Soc no. 21:2262–71.

Pacheco-Sanchez, M., Y. Boutin, P. Angers, A. Gosselin, and R.J. Tweddell. 2006. "A bioactive (1R3)-, (1R4)-b-D-glucan from Collybia dryophila and other mushrooms." Mycologia no. 98 (2):180–185.

Petrova, R. D., J. Mahajna, S. P. Wasser, N. Ruimi, C. M. Denchev, S. Sussan, E. Nevo, and A. Z. Reznick. 2009 "Marasmius oreades substances block NF-κB activity through interference with IKK activation pathway " Mol Biol Rep . 36:737–744.

Patrick, M., M.W. Adlard, and T. Keshavarz. 1993. "Production of an indolizidine alkaloid, swainsonine by the filamentous fungus Metarhizium anisopliae." Biotechnol Lett no. 15:997-1000.

Pizzi M, Liou HC, Sweatt JD. A bioinformatics analysis of memory consolidation reveals involvement of the transcription factor c-rel. J. Neurosci.2004; 24 (16): 3933–43.

Pedras, M. S., L. Irina Zaharia, and D. E. Ward. 2002. "The destruxins: synthesis, biosynthesis, biotransformation, and biological activity." Phytochemistry no. 59 (6):579-96.

Peng, K. C., H. S. Huang, Y. M. Tzeng, and S. Y. Liu. 2005. "Circular dichroism analysis of destruxins from Metarhizium anisopliae." J Biochem Biophys Methods . 62 (1):41-50..2004.08.001.

Pahl H. Activators and target genes of Rel/NF-jB transcriptionfactors. Oncogene. 1999; 18: 6853–6866

Reiser. Synthetic Studies towards Mniopetals (III). Solid Phase Organic Synthesis of a Key Intermediate for Combinatorial Synthesis of Mniopetals, Kuehneromycins and Marasmanes. edited by Johann. 2000.

Reiser U, Jauch J. Synthetic Studies towards Mniopetals (III). Solid Phase Organic Synthesis of a Key Intermediate for Combinatorial Synthesis of Mniopetals, Kuehneromycins and marasmanes.2013;1410-1414

Sabareesh, V., RS. Ranganayaki, S. Raghothama, MP Bopanna, H. Balaram, and M.C. Srinivasan. 2007. "Identification and characterization of a library of microheterogeneous cyclohexadepsipeptidesfrom the fungus Isaria." J Nat Prod no. 70:715–29.

Sarabia, F., S. Chammaa, A. S. Ruiz, L. M. Ortiz, and F. J. Herrera. 2004. "Chemistry and biology of cyclic depsipeptides of medicinal and biological interest." Curr Med Chem . 11 (10):1309-32.

Skrobek, A., and T. M. Butt. 2005. "Toxicity testing of destruxins and crude extracts from the insect-pathogenic fungus Metarhizium anisopliae." FEMS Microbiol Lett . 251 (1):23-8.femsle.2005.07.029.

Soto-Cerrato, Vanessa, Beatriz Montaner, Marc Martinell, Marta Vilaseca, Ernest Giralt, and Ricardo Pérez-Tomás. 2005. "Cell cycle arrest and proapoptotic effects of the anticancer cyclodepsipeptide serratamolide (AT514) are independent of p53 status in breast cancer cells." Biochemical Pharmacology no. 71 (1–2):32-41

Suzuki, A., K. Kawakami, and S. Tamura. 1971. "Isolation and structure elucidation of three new insecticidal cyclodepsipeptides, destruxins C and D and desmethyldestruxin B, produced by Metarhizium anisopliae. ." Agric Biol Chem no. 35:1641–3.

Suzuki Y, Ohara A, Sugaya K, Takao K.-I.; Tadano,K.-I. Tetrahedron 2001, 57, 7291

Sister HD, Control of fungal diseases by compounds acting as antipenetrants. Crop Protection, 1986;5(5):306-313

Tsunoo, A., M. Kamijo, N. Taketomo, Y. Sato, and K. Ajisaka. 1997. "Roseocardin, a novel cardiotonic cyclodepsipeptide from Trichothecium roseum TT103." J Antibiot (Tokyo). 50 (12):1007-13.

Uchida, R., R. Imasato, Y. Yamaguchi, R. Masuma, K. Shiomi, H. Tomoda, and S. Omura. 2005. "New insecticidal antibiotics, hydroxyfungerins A and B, produced by Metarhizium sp. FKI-1079." J Antibiot (Tokyo) . 58 (12):804-9. doi: 10.1038/ja.2005.107.

Vázquez, M. J., M. I. Albarrán, A. Espada, A. Rivera-Sagredo, E. Díez, and J. A. Hueso-Rodríguez. 2005. "A new destruxin as inhibitor of vacuolar-type H+-ATPase of Saccharomyces cerevisiae." Chem Biodivers no. 2 (1):123-30.

Velten R, Klostermeyer D, Steffan B, Steglich W, Kuschel A,Anke T.The mniopetals, new inhibitors of reverse transcriptases from a Mniopetalum species (basidiomycetes). II. Structure elucidation.J. Antibiot. 1994;47:1017.

Velten R, Steglich W, Anke T. Determination of absolute configuration a tetracyclic drimane sesquterpenoid by Mosher’s method
Tetrahedron: Asymmetry. 1994; 5: 1229-1232

Wdoshuk CP, Action of the antipenetrant, tricyclazole, on appresoria of Pyrucularia oryzae Physiol. Plant Pathol.1983; 23:245-259

Wasser SP, Weis AL. Medicinal properties of substances occurring in higher basidiomycetes mushrooms: current perspectives (Review). Int J Med Mushr. 1999a;1:31–62

Wasser SP,Weis AL. Therapeutic effects of substances occurring in higher basidiomycetes mushrooms: a modern perspective. Crit Rev Immunol. 1999b;19:65–96

Weihrather J, Johann J. Total Synthesis of Mniopetals A, B, C and D. Synlett. 2013; 24: 1410–1414

Wasser SP, Weis AL.Medicinal Properties of Substances Occurring in higher basidiomycetes Mushrooms: Current Perspectives (Review). Int J of Med Mushrooms. 1999; 1(1) :31-62.

Werner EG, Wray WV, Lin W, Proksch PO. Drimane Sesquiterpenoids from the Fungus Aspergillus ustus Isolated from the Marine Sponge Suberites domuncula J. Nat. Prod. 2009; 72:1585–1588

Yilmaz, Necmettin, Solmaz M , İbrahim Türkekul, Elmastaş M. "Fatty acid composition in some wild edible mushrooms growing in the middle Black Sea region of Turkey." Food Chemistry . 2006;99 (1):168-174.

Yoshito T, Dai Y, Tohru T, Masashi I, Jun-ichi S, Kiyomi K, Kazuya K, Yoshinobu O. Synthesis of (+-)-Marasmic Acid via 1 -oxaspirohexane, Rearrangement J. Am. Chem. SOC19 90; 112:115-119

Yilmaz N, Solmaz M, Türkekul I, Elmastaş M. Fatty acid composition in some wild edible mushrooms growing in the middle Black Sea region of Turkey. Food Chem. 2006;99: 168-174

Zandi E, Karin M. Bridging the gap: composition, regulation,and physiological function of the IκB kinase complex.Mol Cell Biol. 1999 ;19:4547–4551

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
1. 田君美,「中國大陸「臺灣農民創業園」之發展現況與對臺灣之影響」,臺灣銀行季刊(2011年12月),第62卷第4期,頁113-139。
2. 卓仕文,「臺灣農會政治角色的歷史變遷」,大葉大學通識教育學報,第8期(100年12月),頁37-52。
3. 徐遵慈,「當前臺灣面對之經濟安全課題與因應對策」,經濟前瞻,第128期(2010年3月),頁59-63。
4. 涂勳,「兩岸農業交流現況與展望」,國家政策論壇,第2卷第5期(2002年5月),頁31-36。
5. 耿曙,「經濟扭轉政治?中共「惠台政策」的政治影響」,問題與研究,第48卷第3期(2009年9月),頁1-32。
6. 耿曙、曾于蓁,「中共邀訪台灣青年政策的政治影響」,問題與研究,第49卷第3期(2010年9月),頁29-70。
7. 曾偉君、李欣恩,「台灣水稻田之糧食安全及景觀價值」,農業經濟半年刊,第78期(2005年12月),頁39-79。
8. 楊開煌、劉祥得,「社會接觸及政治態度影響臺灣民眾對大陸印象、認知、政策評估之分析」,遠景基金會季刊,12卷3期(2011年7月),頁45-94。
9. 廖坤榮,「台灣農會的社會資本形成與政策績效」,政治科學論叢,第22期(2004年12月),頁181-220。
10. 趙永茂、黃瓊文,「台灣威權體制轉型前後農會派系特質變遷之研究─雲林縣水林鄉農會一九七○及一九九○年代為例之比較分析」,政治科學論叢,第13期(2000年12月),頁165-200。
11. 高穗生、蔡勇勝,「蟲生病源真菌在害蟲防治上之利用」,藥試所專題報導,(1995)38: 4-11。
12. 高穗生、蔡勇勝「蟲生病源真菌在害蟲防治上之利用」,藥試所專題報導,(1996) 39:1-16。