(34.239.150.57) 您好!臺灣時間:2021/04/14 22:57
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:莊凱荃
研究生(外文):CHUANG KAI CHUAN
論文名稱:養殖雨生紅球藻生產蝦青素的研究
論文名稱(外文):The Studies of Haematococcus pluvialis Cultivation for Astaxanthin Production
指導教授:張振昌
口試委員:吳柔賢賴奇厚
口試日期:2014-07-17
學位類別:碩士
校院名稱:逢甲大學
系所名稱:化學工程學系
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:109
中文關鍵詞:雨生紅球藻蝦青素直立式光合反應器
外文關鍵詞:Haematococcus pluvialisAstaxanthinVertical tubular photobioreactor
相關次數:
  • 被引用被引用:0
  • 點閱點閱:3831
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:63
  • 收藏至我的研究室書目清單書目收藏:0
雨生紅球藻富含天然蝦青素,具有高抗氧化性,可去除自由基, 提高免疫力、抗老及抗癌的效果,是迄今為止人類發現自然界最強的 抗氧化劑,遠超過其他的種類的抗氧化劑。本研究分兩階段模式培養 雨生紅球藻,在第一階段策略培養前先進行小量批次培養,發現定時 更換新鮮培養液能有效幫助雨生紅球藻生長。第一階段策略培養中, 設計一套田口實驗方法尋找養殖雨生紅球藻最佳培養條件,實驗結果 以培養基 JM 的濃度為基礎,其中氮、碳、磷濃度比為 1:2:5 時,並 在紅光 LED 照射下,光照強度為 1600 lux,培養 10 天,可得最佳生 長速率為 0.262 d-1。在第二階段策略培養中,實驗結果顯示,以缺乏 主要氮源之培養基 JM 培養並採藍光 LED 照射,光照強度為 8000 lux 下可加速雨生紅球藻由綠轉紅,可得到蝦青素含量約 3.2 mg L-1。使 用直立式光合反應器培養,採未循環的方式培養,培養 7 天後,藻密 度達 50×104 cell mL-1,生長速率可達到 0.291 d-1。
Haematococcus pluvialis contain with abundant natural astaxanthin, which is full of antioxidant capacity that can quench free radicals, improve immune system, and prevent aging and cancer. Up to now, natural astaxanthin has been discovered as a most powerful antioxidant in natural resources. In this study, it’s divided into two steps to cultivate Haematococcus pluvialis. Before the first step, we found that replacing fresh medium regularly can nurture Haematococcus pluvialis effectively. In the first step of culture, use Taiguchi method to search for the optimal experimental condition for Haematococcus pluvialis production. The result shows that the optimum growth rate is 0.262 d-1, with the medium (JM) in N:C:P=1:2:5, irradiation of red light LED at illumination of 1600 lux, culturing for 10 days. In the second step of culture, it shows that the color in Haematococcus pluvialis will be accelerated from green to red, and get the concentration of Astaxanthin about 3.2 mg L-1, with the medium (JM), which is without main Nitrogen, irradiation of blue light LED at illumination of 8000 lux. Using vertical tubular photobioreactor to cultive Haematococcus pluvialis for 7 days in un-circulated condition, the concentration of algae reached 50×104 cell mL-1. As a result, the growth rate of algae was 0.291 d-1.
致 謝 I
摘要 II
Abstract III
目錄 IV
圖目錄 IX
表目錄 XII
第一章 緒論 1
1-1 研究動機 1
1-2 研究目的 4
第二章 文獻回顧 5
2-1 藻類 5
2-2 現代藻類的應用 5
2-2-1 能源 5
2-2-2 保健與營養食品 7
2-2-2-1 藻體 8
2-2-2-2 萃取物 10
2-3 培養方式 13
2-3-1 自營生長 13
2-3-2 異營生長 14
2-3-3 混營生長 15
2-3-4 二階段策略生長 15
2-4 雨生紅球藻(Haematococcus pluvialis) 16
2-5 蝦青素(Astaxanthin) 17
2-5-1 由水產生物提取蝦青素 18
2-5-2 由酵母菌提取蝦青素 19
2-5-3 由藻類提取蝦青素 19
2-6 影響雨生紅球藻生長因子 20
2-6-1 pH值 20
2-6-2 光源 21
2-6-3 溫度 22
2-6-4 營養鹽與微量元素 23
2-7 二階段策略培養雨生紅球藻 24
2-8 培養反應器 25
2-8-1 開放式培養系統 25
2-8-2 封閉式培養系統 27
2-8-3 現階段培養雨生紅球藻之系統 36
第三章 實驗方法與實驗流程 40
3-1 實驗目的及方法 40
3-2 實驗規劃 41
3-3 培養基組成 41
3-4 培養反應器 43
3-5 種源與其來源 47
3-5-1 種原的純化與保存 47
3-5-2 小量活性培養及放大 49
3-6 實驗流程 51
3-6-1 光合混營之培養 51
3-6-1-1 雨生紅球藻細胞大量化 51
3-6-1-2 雨生紅球藻迅速累積蝦青素 52
3-6-2 光合反應器培養 52
3-6-3 藻體收集及乾燥 53
3-6-4 培養條件 54
3-7 實驗分析 55
3-7-1 細胞數測定(Cells Number) 55
3-7-2 藻體密度測定(Dry Weight) 55
3-7-3 光學密度測定(Optical Density) 55
3-7-4 pH測定 56
3-7-5 光照強度測定 56
3-7-6 生長速率測定 56
3-7-7 蝦青素濃度測定 57
3-7-7-1 蝦青素之特徵峰 58
3-7-7-2 蝦青素之檢量線 58
3-8 田口實驗設計 60
第四章 實驗結果與討論 63
4-1 種原處理及逐步放大 63
4-1-1 固態保種 63
4-1-2 小量活性培養及放大 65
4-2 雨生紅球藻細胞大量化 67
4-2-1 營養鹽濃度對一階段策略培養之影響 67
4-2-2 光源對一階段策略培養之影響 69
4-2-3 採用最佳條件培養雨生紅球藻 71
4-3 雨生紅球藻迅速累積蝦青素 74
4-3-1 不同光照強度累積蝦青素之關係 74
4-3-2 藻細胞密度變化與蝦青素累積之關係 78
4-3-3 pH變化與蝦青素累積之關係 79
4-4 光合反應器 80
4-4-1 單一管培養雨生紅球藻 81
4-4-2 光合反應器操作之可行性 84
第五章 結論與未來展望 89
附錄 91
附錄A: 實驗化學藥品與儀器 91
附錄A-1: 實驗化學藥品 91
附錄A-2: 實驗儀器 93
附錄A-3: 藥品儀器供應商資料 94
附錄B: 光源的波長及能量密度分析 96
附錄B-1: 紅光LED 96
附錄B-2: 綠光LED 97
附錄B-3: 藍光LED 98
附錄B-5 T5日光燈管 99
參考文獻 100
1.徐明光, 台灣的淡水浮游藻(I)—通論及綠藻(1). 國立台灣博物館出版, 1999.
2.曲新生, 何無忌, 李宏台, 李麗玲, 吳 煌, 邱錦松, 胡耀祖, 柳志錫, 許銘修, 梁佩芳, 黃正忠, 張永源, 張鈺炯, 黃怡碩, 陳秋麟, 陳錫銓, 張克勤, 童遷祥, 詹益亮, 楊昌中, 劉子衙, 劉振邦, 鄭名山, 蔡松雨, 歐陽湘, 顏志偉, and 羅新衡, 2012年能源產業技術白皮書. 經濟部能源局出版, 2012, p. 17.
3.Colin Barrow, Fereidoon Shahidi, Marine nutraceuticals and functional foods. CRC Press, 2007.
4.Barbara C. Benson, Maria T. Gutierrez-Wing, Kelly A. Rusch, Optimization of the lighting system for a Hydraulically Integrated Serial Turbidostat Algal Reactor (HISTAR): economic implications. Aquacultural Engineering, 2009. 40(1): p. 45-53.
5.Kamonpan Kaewpintong, Artiwan Shotipruk, Sorawit Powtongsook, Prasert Pavasant, Photoautotrophic high-density cultivation of vegetative cells of Haematococcus pluvialis in airlift bioreactor. Bioresource Technology, 2007. 98(2): p. 288-295.
6.T Krawczyk, Biodiesel – Alternative fuel makes inroads but hurdles remain. INFORM, 1996(7): p. 801–829.
7.Chisti Yusuf, Biodiesel from microalgae. Biotechnology Advances, 2007. 25(3): p. 294-306.
8.Dan Somma, Hope Lobkowicz, Jonathan P. Deason, Growing America’s fuel: an analysis of corn and cellulosic ethanol feasibility in the United States. Clean Technologies and Environmental Policy, 2010. 12(4): p. 373-380.
9.Hua-Jiang Huang, Shri Ramaswamy, Waleed Al-Dajani, Ulrike Tschirner, Richard A. Cairncross, Effect of biomass species and plant size on cellulosic ethanol: a comparative process and economic analysis. Biomass and Bioenergy, 2009. 33(2): p. 234-246.
10.Seung Gon Wi, Hyun Joo Kim, Shobana Arumugam Mahadevan, Duck-Joo Yang, Hyeun-Jong Bae, The potential value of the seaweed Ceylon moss (Gelidium amansii) as an alternative bioenergy resource. Bioresource Technology, 2009. 100(24): p. 6658-6660.
11.張嘉修, 生質氫能. 科學發展, 2009(433): p. 32-35.
12.Carlos Jiménez, Belén R. Cossı́o, F. Xavier Niell, Relationship between physicochemical variables and productivity in open ponds for the production of Spirulina : a predictive model of algal yield. Aquaculture, 2003. 221(1): p. 331-345.
13.陳東林, 螺旋藻的生物化学分析及开发利用价值研究. 宜賓學院學報, 1997(2): p. 90-93.
14.Ikuo Saiki, Toshimitsu Kato, Jun Murata, Hideki Fujii, Inhibition of Tumor Invasion and Metastasis by Calcium Spirulan (Ca-SP), a Novel Sulfated Polysaccharide Derived from a Blue-Green Alga Spirulina Platensis. Nutritional Sciences, 2004. 7.
15.張立彬, 甄二英, 李振永, 螺旋藻的營養價值及培養. 飼料研究, 2006. 2006(1): p. 31-32.
16.Teresa M. Mata, António A. Martins, Nidia S. Caetano, Microalgae for biodiesel production and other applications: A review. Renewable and Sustainable Energy Reviews, 2010. 14(1): p. 217-232.
17.Sheng-Yi Chiu, Chien-Ya Kao, Chiun-Hsun Chen, Tang-Ching Kuan, Seow-Chin Ong, Chih-Sheng Lin, Reduction of CO2 by a high-density culture of Chlorella sp. in a semicontinuous photobioreactor. Bioresource Technology, 2008. 99(9): p. 3389-3396.
18.Chih Hung Hsieh, Wen Teng Wu, Cultivation of microalgae for oil production with a cultivation strategy of urea limitation. Bioresource Technology, 2009. 100(17): p. 3921-3926.
19.詹明章, 本土微藻生產葉黃素之技術開發–微藻培養, 萃取方法, 保存條件及產量程序最適化. 碩士論文, 化學工程學系, 國立台灣成功大學, 2012.
20.吴蕾, 庞广昌, 陈庆森, 螺旋藻藻蓝蛋白的规模化提取和色谱纯化技术研究进展. 食品科学, 2008. 29(4): p. 461-463.
21.张唐伟, 李天才, 藻胆蛋白质的提取纯化与生物活性研究进展. Biotechnology Bulletin, 2010(1): p. 9-13.
22.R. Sarada, Manoj G. Pillai, G. A. Ravishankar, Phycocyanin from Spirulina sp: influence of processing of biomass on phycocyanin yield, analysis of efficacy of extraction methods and stability studies on phycocyanin. Process Biochemistry, 1999. 34(8): p. 795-801.
23.Lu-Ning Liu, Xiu-Lan Chen, Xi-Ying Zhang, Yu-Zhong Zhang, Bai-Cheng Zhou, One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata. Journal of Biotechnology, 2005. 116(1): p. 91-100.
24.Geoffrey A. Codd, Louise F. Morrison, James S. Metcalf, Cyanobacterial toxins: risk management for health protection. Toxicology and Applied Pharmacology, 2005. 203(3): p. 264-272.
25.José A. Del Campo, José Moreno, Herminia Rodrı́guez, M. Angeles Vargas, Joaquı́n Rivas, Miguel G. Guerrero, Carotenoid content of chlorophycean microalgae: factors determining lutein accumulation in Muriellopsis sp.(Chlorophyta). Journal of Biotechnology, 2000. 76(1): p. 51-59.
26.洪啟峰, 葉黃素及玉米黃素在皮膚保養的應用. 健康世界, 2007(263): p. 82-83.
27.陳瑩山, 眼睛也會 [骨質流失] 嗎?-淺談黃斑部的 [葉黃素低下]. 健康世界, 2013(333): p. 55-59.
28.Xian Ming Shi, Xue Wu Zhang, Feng Chen, Heterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sources. Enzyme and Microbial Technology, 2000. 27(3): p. 312-318.
29.A. Jassby, Spirulina: A Model for Microalgae as Human Food. in: C.A. Lumb, J.R. Waaland (Eds.), Algae and Human Affairs, Cambridge University Press,Cambridge. 1988, 149–179.
30.趙強, 對抗疾病與老化的新發現--自由基與抗氧化物質. 美食天下 1997(64): p. 116.
31.Earl S. Ford, Julie C. Will, Barbara A. Bowman, K. M. Venkat Narayan, Diabetes mellitus and serum carotenoids: findings from the Third National Health and Nutrition Examination Survey. American Journal of Epidemiology, 1999. 149(2): p. 168-176.
32.葉姝蘭, beta-胡蘿蔔素,茄紅素和氧化反應:體外試驗抗氧化、促氧化效應及對細胞奸細聯繫之抑制. 博士論文, 食品科學系, 國立中興大學, 2002.
33.Winsome Abbott-Johnson, Paul Kerlin, Chapter 30 - Vitamin A, Zinc, Dark Adaptation, and Liver Disease, in Handbook of Nutrition, Diet and the Eye, Victor R. Preedy, Editor. 2014, Academic Press: San Diego. p. 301-310.
34.Eileen E. Birch, Dennis R. Hoffman, Ricardo Uauy, David G. Birch, Claude Prestidge, Visual acuity and the essentiality of docosahexaenoic acid and arachidonic acid in the diet of term infants. Pediatric Research, 1998. 44(2): p. 201-209.
35.B. Koletzko, M. Braun, Arachidonic acid and early human growth: is there a relation? , Annals of Nutrition and Metabolism, 1991. 35(3): p. 128-131.
36.Carlo Agostoni, Enrica Riva, R. Bellu, Sabina Trojan, Diego Luotti, Marcello Giovannini, Effects of diet on the lipid and fatty acid status of full-term infants at 4 months. Journal of the American College of Nutrition, 1994. 13(6): p. 658-664.
37.Maria Makrides, Mark A. Neumann, Brett Jeffrey, Eric L. Lien, Robert A. Gibson, A randomized trial of different ratios of linoleic to α-linolenic acid in the diet of term infants: effects on visual function and growth. The American Journal of Clinical Nutrition, 2000. 71(1): p. 120-129.
38.王利华, 霍贵成, ω—3 不饱和和脂肪酸的生物学作用. 东北农业大学学报, 2001. 32(1): p. 100-104.
39.J. Stewart Forsyth, Susan E. Carlson, Long-chain polyunsaturated fatty acids in infant nutrition: effects on infant development. Current Opinion in Clinical Nutrition &; Metabolic Care, 2001. 4(2): p. 123-126.
40.B. W. Nichols, R. S. Appleby, The distribution and biosynthesis of arachidonic acid in algae. Phytochemistry, 1969. 8(10): p. 1907-1915.
41.Trevor A. Mori, Danny Q. Bao, Valerie Burke, Ian B. Puddey, Lawrence J. Beilin, Docosahexaenoic acid but not eicosapentaenoic acid lowers ambulatory blood pressure and heart rate in humans. Hypertension, 1999. 34(2): p. 253-260.
42.David S. Siscovick, T. E. Raghunathan, Irena King, Sheila Weinmann, Kristine G. Wicklund, Jennifer Albright, Viktor Bovbjerg, Patrick Arbogast, Heidi Smith, Lawrence H. Kushi, Dietary intake and cell membrane levels of long-chain n-3 polyunsaturated fatty acids and the risk of primary cardiac arrest. Jama, 1995. 274(17): p. 1363-1367.
43.K. Umemura, Y. Toshima, F. Asai, M. Nakashima, Effect of dietary docosahexaenoic acid in the rat middle cerebral artery thrombosis model. Thrombosis Research, 1995. 78(5): p. 379-387.
44.萧家捷, DHA 和 EPA 的功能综述. 中国食物与营养, 1996(2): p. 8-11.
45.John Paul SanGiovanni, Emily Y. Chew, The role of omega-3 long-chain polyunsaturated fatty acids in health and disease of the retina. Progress in Retinal and Eye Research, 2005. 24(1): p. 87-138.
46.林榮芳, 黃檀溪, 比較耐熱性小球藻異營生長之特性. 師大學報數理與科技類, 2002. 47(1).
47.Hiroshi Endo, Hideo Hosoya, Tamotsu Koibuchi, Growth Yields of Chlorella regularis in Dark-heterotrophic Continuous Cultures Using Acetate: Studies on Chlorella regularis, Heterotrophic Fast-Growing Strain (III). Journal of Fermentation Technology, 1977. 55(4): p. 369-379.
48.Gilles Lalibertè, Joël Noüie, Auto-, hetero-, and mixotrophic growth of Chlamydomonas humicola (Chlorophyceae) on acetate. Journal of Phycology, 1993. 29(5): p. 612-620.
49.Makio Kobayashi, Toshihide Kakizono, Kazuya Yamaguchi, Naomichi Nishio, Shiro Nagai, Growth and astaxanthin formation of Haematococcus pluvialis in heterotrophic and mixotrophic conditions. Journal of Fermentation and Bioengineering, 1992. 74(1): p. 17-20.
50.Keiko Yamaberi, Mutsumi Takagi, Toshiomi Yoshida, Nitrogen depletion for intracellular triglyceride accumulation to enhance liquefaction yield of marine microalgal cells into a fuel oil. Journal of Marine Biotechnology, 1998. 6: p. 44-48.
51.吳佳育, 兩階段培養微藻 Chlorella sp. 生產油脂之研究. 碩士論文, 化學工程學系, 國立清華大學, 2010. p.1-76
52.林佳蓉, 微藻養殖模式對微藻油脂含量與組成影響之探討. 碩士論文, 分子醫學與生物工程研究所, 國立交通大學, 2011. p.1-88
53.Jaime Fábregas, Adolfo Domínguez, Digna García Álvarez, Teresa Lamela, Ana Otero, Induction of astaxanthin accumulation by nitrogen and magnesium deficiencies in Haematococcus pluvialis. Biotechnology Letters, 1998. 20(6): p. 623-626.
54.Sammy Boussiba, Avigad Vonshak, Astaxanthin accumulation in the green alga Haematococcus pluvialis1. Plant and Cell Physiology, 1991. 32(7): p. 1077-1082.
55.Mark Harker, Alex J. Tsavalos, Andrew J. Young, Autotrophic growth and carotenoid production of Haematococcus pluvialis in a 30 liter air-lift photobioreactor. Journal of Fermentation and Bioengineering, 1996. 82(2): p. 113-118.
56.PZ Margalith, Production of ketocarotenoids by microalgae. Applied Microbiology and Biotechnology, 1999. 51(4): p. 431-438.
57.Y. K. Lee, D. H. Zhang, Production of astaxanthin by Haematococcus. Chemicals from Microalgae, 1999: p. 173-195.
58.劉建國, 殷明焱, 張京浦, 孟昭才, W. F. Bourne, 雨生紅球藻的細胞周期初探. 海洋與湖沼, 2000. 31(2): p. 145-150.
59.Elliot, A.M., Morphology and life history of Haematococcus pluvialis. Arch. Protistenk., 1934. 82: p. 250-272.
60.Lu Fan, Avigad Vonshak, Sammy Boussiba, Effect of temperature and irradiance on growth of Haematococcus pluvialis (chlorophyceae). Journal of Phycology, 1994. 30(5): p. 829-833.
61.R. Todd Lorenz, Gerald R. Cysewski, Commercial potential for Haematococcus microalgae as a natural source of astaxanthin. Trends in Biotechnology, 2000. 18(4): p. 160-167.
62.Su-Lim Choi, In Soo Suh, Choul-Gyun Lee, Lumostatic operation of bubble column photobioreactors for Haematococcus pluvialis cultures using a specific light uptake rate as a control parameter. Enzyme and Microbial Technology, 2003. 33(4): p. 403-409.
63.Yousry MA Naguib, Antioxidant activities of astaxanthin and related carotenoids. Journal of Agricultural and Food Chemistry, 2000. 48(4): p. 1150-1154.
64.Kiyotaka Nakagawa, Sung-Do Kang, Dong-Ki Park, Garry J Handelman, Teruo Miyazawa, Inhibition of beta-carotene and astaxanthin of NADPH-dependent microsomal phospholipid peroxidation. Journal of Nutritional Science and Vitaminology, 1997. 43(3): p. 345-355.
65.Yasuhiro Nishida, Eiji Yamashita, Wataru Miki, Quenching activities of common hydrophilic and lipophilic antioxidants against singlet oxygen using chemiluminescence detection system. Carotenoid Science, 2007. 11: p. 16-20.
66.Eric A. Johnson, Gil-Hwan An, Astaxanthin from microbial sources. Critical Reviews in Biotechnology, 1991. 11(4): p. 297-326.
67.Gil-Hwan An, Byung-Gui Jang, Myung-Haing Cho, Cultivation of the carotenoid-hyperproducing mutant 2A2N of the red yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) with molasses. Journal of bioscience and bioengineering, 2001. 92(2): p. 121-125.
68.Namkyu Sun, Seunghee Lee, Kyung Bin Song, Characterization of a carotenoid-hyperproducing yeast mutant isolated by low-dose gamma irradiation. International Journal of Food Microbiology, 2004. 94(3): p. 263-267.
69.F Camacho Rubio, FG Fernandez, JA Perez, F García Camacho, E Molina Grima, Prediction of dissolved oxygen and carbon dioxide concentration profiles in tubular photobioreactors for microalgal culture. Biotechnology and Bioengineering, 1999. 62(1): p. 71-86.
70.MJ Merrett, NA Nimer, LF Dong, The utilization of bicarbonate ions by the marine microalga Nannochloropsis oculata (Droop) Hibberd. Plant, Cell &; Environment, 1996. 19(4): p. 478-484.
71.Becker E. Wolfgang, Microalgae: biotechnology and microbiology. Vol. 10. 1994.
72.曹平华, 李晓霞, 禹学礼, 陶林, 雨生红球藻在不同培养基中的生长效果比较. 河南农业科学, 2004(4): p. 65-68.
73.邱保胜, 刘其芳, 雨生红球藻(Haematoccus pluvialis-6B)培养条件研究. 華中師範大學學報, 1999. 33(1): p. 112-118.
74.殷明焱, 刘建国, 雨生红球藻和虾青素研究述评. 海洋湖沼通报, 1998(2): p. 53-62.
75.沈渊, 蔡明刚, 黄水英, 石荣贵, 李哲, 陆晓霞, 利用光生物反应器培养雨生红球藻的研究初探. 海洋科学, 2010. 34(10): p. 83-89.
76.Jirı Masojıdek, Michal Koblızek, Giuseppe Torzillo, 2 Photosynthesis in Microalgae. Handbook of Microalgal Culture: Biotechnology and Applied Phycology. 2004, 20.
77.Tao You, Barnett, Stanley M., Effect of light quality on production of extracellular polysaccharides and growth rate of Porphyridium cruentum. Biochemical Engineering Journal, 2004. 19(3): p. 251-258.
78.Mark Harker, Alex J Tsavalos, Andrew J Young, Use of response surface methodology to optimise carotenogenesis in the microalga, Haematococcus pluvialis. Journal of Applied Phycology, 1995. 7(4): p. 399-406.
79.Mark Harker, Alex J Tsavalos, Andrew J Young, Factors responsible for astaxanthin formation in the Chlorophyte Haematococcus pluvialis. Bioresource Technology, 1996. 55(3): p. 207-214.
80.韩博平, 韩志国, 付翔, 藻类光合作用机理与模型. 科学出版社, 2003, p.232.
81.Tomohisa Katsuda, Abdolmajid Lababpour, Kazumichi Shimahara, Shigeo Katoh, Astaxanthin production by Haematococcus pluvialis under illumination with LEDs. Enzyme and Microbial Technology, 2004. 35(1): p. 81-86.
82.姚銘輝, 光度單位轉換問題之探討. 農業試驗所技術服務, 2011(85): p. 26-29.
83.謝誌鴻, 吳文騰, 微藻—綠色生質能源. 科學發展, 2009. 433: p. 36-40.
84.Susan M. Renaud, Luong-Van Thinh, George Lambrinidis, David L. Parry, Effect of temperature on growth, chemical composition and fatty acid composition of tropical Australian microalgae grown in batch cultures. Aquaculture, 2002. 211(1–4): p. 195-214.
85.張惟閔, 微藻培養於新型光生化反應器之系統開發. 碩士論文, 化學工程學系, 國立清華大學, 2005. p. 59
86.Sammy Boussiba, Lu Fan, Avigad Vonshak, Enhancement and determination of astaxanthin accumulation in green alga Haematococcus pluvialis. Methods in Enzymology, 1992. 213: p. 386-391.
87.陈兴才, 黄伟光, 欧阳琴, 雨生红球藻培养及虾青素累积条件探讨. 中国食品学报, 2006. 6(4): p. 41-45.
88.David H. Turpin, Effects of inorganic N availability on algal photosynthesis and carbon metabolism. Journal of Phycology, 1991. 27(1): p. 14-20.
89.Facundo J. Marquez, Ken Sasaki, Toshihide Kakizono, Naomichi Nishio, Shiro Nagai, Growth characteristics of Spirulina platensis in mixotrophic and heterotrophic conditions. Journal of Fermentation and Bioengineering, 1993. 76(5): p. 408-410.
90.Colin Ratledge, Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie, 2004. 86(11): p. 807-815.
91.上野洋一郎, 柯俊良, 陳怡菁, 郭順宇, 郭榮烈, 陳泓志, 胡威文, 張傳偉, 植物組織培養技術. 藝軒圖書出版社, 2000.
92.袁玉信, 微量元素在植物生活中的作用. 生物学通报, 1996. 31(4): p. 4-8.
93.陈小霞, 吴振强, 藻类对微量元素的生物富集及其机理探讨. 食品与发酵工业, 1999. 25(4): p. 56-60.
94.Yuan‐Kun Lee, Sun‐Yeun Ding, Cell cycle and accumulation of astaxanthin in Haematococcus lacustris (Chlorophyta). Journal of Phycology, 1994. 30(3): p. 445-449.
95.黄水英, 齐安翔, 李哲, 李晓梦, 王蕴, 蔡明刚, 几种胁迫方式对雨生红球藻积累虾青素影响的初步研究. 海洋科學集刊, 2009(49): p. 144-151.
96.李哲, 蔡明刚, 李晓梦, 齐安翔, 王蕴, 黄水英, 不同波段可见光对雨生红球藻生长与虾青素积累的影响, in 中国海洋湖沼学会第九次全国会员代表大会暨学术研讨会论文摘要汇编. 中国海洋湖沼学会. 2007:194
97.Michael A. Borowitzka, Commercial production of microalgae: ponds, tanks, and fermenters. Progress in Industrial Microbiology, 1999. 35: p. 313-321.
98.Fernando Olmedo. Seambiotic USA y NASA Glenn Research Center firman acuerdo para optimizar procesos de microalgas a gran escala. 2009 [cited 2014 6.4]; Available from: http://www.biodisol.com/biocombustibles/seambiotic-usa-y-nasa-glenn-research-center-firman-acuerdo-para-optimizar-procesos-de-microalgas-a-gran-escala-energias-renovables-biocombustibles-cultivos-energeticos/
99.Giuseppe Torzillo, Tubular bioreactors. Spirulina platensis (Arthrospira): Physiology, Cell-Biology and Biotechnology. Taylor &; Francis, London, 1997: p. 101-115.
100.许波, 王长海, 微藻的平板式光生物反应器高密度培养. 食品与发酵工业, 2003. 29(1): p. 36-40.
101.林志生, 邱聖壹, 光生物反應器於微藻培養之研究與產業化的進展. 動物與水產生技, 2010(22): p. 44-51.
102.Ana P. Carvalho, Luís A. Meireles, F. Xavier Malcata, Microalgal reactors: a review of enclosed system designs and performances. Biotechnology Progress, 2006. 22(6): p. 1490-1506.
103.Inc. NanoVoltaic. Algae Photobioreactors. [cited 2014 6.4]; Available from: http://www.nanovoltaics.com/content/algae-photobioreactors
104.Toru Sato, Shinsuke Usui, Yoshihiro Tsuchiya, Yutaka Kondo, Invention of outdoor closed type photobioreactor for microalgae. Energy Conversion and Management, 2006. 47(6): p. 791-799.
105.張嘉修, 林志生, 陳俊延, 高千雅, 具有二氧化碳補集功能之為藻養殖裝置及方法, TW201408192, 中華民國, 2014
106.AlgaeLink NV. 2012 [cited 2014 6.4]; Available from: http://www.algaelink.com/joomla/
107.蘇惠美, 微藻生質能技術聯合成果發表會. 水試專訊, 2013(41): p. 54-56.
108.Yuan-Kun Lee, Enclosed bioreactors for the mass cultivation of photosynthetic microorganisms: the future trend. Trends in Biotechnology, 1986. 4(7): p. 186-189.
109.Rédaction. , ExxonMobil veut mettre des algues dans le moteur. 2010 [cited 2014 6.4]; Available from: http://www.enviro2b.com/2010/07/16/exxonmobil-veut-faire-mettre-des-algues-dans-le-moteur/
110.Fuji Chemical Industry Co. AstaReal, Ltd. About AstaREAL. 2004 [cited 2014 6.4]; Available from: http://www.astavita.com/astareal.html
111.陳曉薇, 陳茂景, 陳志聖, 曹志明, 生態循環生產模式--台電公司在「微藻減碳」技術的發展. 能源報導, 2012.10: p. 8.
112.陳茂景, 陳曉薇, 藍啓仁, 洪順祥, 陳銘宗, 螺旋藻立體化光合反應器, M363198 中華民國, 2009
113.劉智淵, 微藻減碳—溫室氣體減量的動人遠景. 台電月刊, 2008. 550: p. 4-15.
114.Wei Dong, Zang Xiaonan, Production of natural astaxanthin by mass cultivation of Haematococcus pluvialis: Research advances and current situation of industrialization. Chinese Journal of Marine Drugs, 2001. 20(5): p. 4-8.
115.B. Sammy, C. Zvi, R. Amos, V. Avigad, Procedure for large-scale production of astaxanthin from haematococcus, US6022701, United States Patents, 1997
116.Algatechologies. 1998 [cited 2014 6.4]; Available from: http://www.algatech.com
117.马建, 陈黎明, 黄胜奎, 裴芳芳, 用于规模化红球藻生产虾青素的光生物反应器, CN202744558, 中華人民共和國, 2012
118.A.S. Thompson, J.C. Rhodes, I. Pettman, Culture Collection of Algae and Protozoa: Catalogue of Strains 1988. 5 ed. Natural Environmental Research Council, 1988.
119.張振昌, 林秋裕, 李奕昇, 劉季樺, 林叡志, 直立式藻類養殖設備, M396612, 中華民國, 2011
120.台灣藻類資源應用研發中心. 微藻種原推廣. 2013 [cited 2014 6.4]; Available from: http://140.121.167.7/wordpress/?page_id=120
121.杨雪梅, 培养密度和培养广&;quot;老化&;quot;对盐藻生长和 B-胡萝卜素累积的影响. 海洋科學, 1996(6): p. 39-43.
連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔