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研究生:高豪傑
研究生(外文):KAO, HAO-CHIEH
論文名稱:分解二苯乙烯型螢光增白劑之微生物的分離與特性分析
論文名稱(外文):The isolation and characterization of micro-organisms for degrading fluorescent whitening agents of stilbene-type
指導教授:呂誌翼
指導教授(外文):LU, CHIH-YI
口試委員:鐘竺均賴弘智
口試委員(外文):CHUNG, CHU-CHUNLAI, HUNG-CHIH
口試日期:2013-06-25
學位類別:碩士
校院名稱:輔仁大學
系所名稱:生命科學系碩士班
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:117
中文關鍵詞:螢光增白劑微生物汙水處理綠色技術
外文關鍵詞:Fluorescent whitening agentsmicrorganismwastewater treatmentgreen technology
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  • 下載下載:8
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螢光增白劑(fluorescent whitening agents)功能為增加潔白的效果,大量被使用於清潔劑相關產品、紡織、造紙、塑膠染色等工業上,已有30 餘年。螢光增白劑能吸收波長300~400nm近紫外線(near ultraviolet rays, NUV),再放射出波長為420~480nm的藍紫色螢光。人造合成螢光增白劑的使用造成環境嚴重的危害,而其中二苯乙烯型螢光增白劑佔大多數的使用量。因此如何處理水體中的螢光增白劑,以及積極研發生物分解性的螢光增白劑,已成為各國研究的重點之一。本研究目的:從環境中分離出具有高效率降解二苯乙烯型螢光增白劑2S(fluorescent brightener 28,FB28)、4S(fluorescent brightener 220,FB220)、6S(fluorescent brightener 357,FB357)的菌株,並進行菌種鑑定和生理、生化特性分析,以及探討降解螢光增白劑2S、4S、6S的效率。實驗中已分離出7株菌株,具有降解2S、4S或6S的能力。實驗利用16S rDNA序列比對分析,初步確認這些菌株的親緣演化,再進一步藉由TLC片、螢光強度、高效液相層析儀探討這些菌株降解2S、4S、6S的效率,希望篩選出具有高效降解2S、4S、6S效率的菌株,以作為處理含有螢光增白劑2S、4S、6S的汙水。在實驗結果發現目標菌株在培養20天具有20~30%的降解率。未來實驗規劃將以反應曲面法(response surface methodology, RSM)來設計環境因子並找出最適降解螢光增白劑的條件,以減少環境污染。
The function of fluorescent whitening agents(FWAs) has been use to increase bright of textiles, paper, plastics and other industrial dyeing, for more than 30 years. FWAs can absorb 300 ~ 400nm of near-ultraviolet rays wavelength, and then emit a blue-violet wavelength(420 ~ 480nm) fluorescence. The use of artificial synthetic stilbene FWAs causes serious damage of natural environments. Therefore, how to deal with FWAs pollution in environment, and develop actively biodegradable FWAs, become one of the important researches. The study is aimed to isolate strains with high efficiency of degrading stilbene fluorescent brightener 2S(fluorescent brightener 28, FB28), 4S(fluorescent brightener 220, FB220), 6S(fluorescent brightener 357, FB357) from a variety of environments. Seven isolates were been identified and characterized with molecular, physiological, and biochemical analyses, and also investigated the degradation ability of FWAs 2S, 4S or 6S. Experiments including TLC plates, Fluorescence intensity, and high performance liquid chromatography(HPLC) to analyze the degradation of 2S, 4S, and 6S were carried out. The result indicate target strains have 20% to 30% degradation rate after 20 days. The future work will design response surface methodology(RSM) simulating environmental factors and find the optimal degrading conditions of fluorescent brightener for reducing environmental pollution by FWAs.
摘要 I
Abstract II
目錄 III
表目錄 VI
圖目錄 VII
一、前言 1
1.1 螢光增白劑特性與應用 1
1.2 螢光增白劑的演變 3
1.3螢光增白劑使用的概況 4
1.4螢光增白劑對生物體的影響 4
1.4.1 皮膚 5
1.4.2 致癌 5
1.4.3抑制傷口癒合 5
1.4.4代謝 6
1.5螢光增白劑對環境的影響 6
1.6 本研究使用的螢光增白劑 7
1.7研究目的 9
二、材料與方法 10
2.1實驗藥品與儀器設備 10
2.1.1實驗藥品 10
2.1.2儀器設備 11
2.2菌株來源與培養 12
2.2.1採樣地點 12
2.2.2菌株來源 13
2.3菌株篩選與培養 13
2.3.1固態篩選法 14
2.3.2液態篩選法 14
2.4實驗所使用之培養基 15
2.5菌種鑑定 17
2.5.1 染色體抽取 17
2.5.2連鎖聚合酶反應(Polymerase Chain Reaction; PCR) 19
2.5.3序列比對分析 19
2.6生長曲線測定 20
2.7生化特性分析 20
2.7.1溫度生長範圍測試 20
2.7.2酸鹼值生長範圍測試 20
2.8螢光增白劑分解測試實驗 21
2.8.1螢光強度測試 21
2.8.2薄層層析法(Thin-Layer Chromatography;TLC)分析方法 22
2.8.3螢光增白劑之高效液相層析儀分析方法 23
2.8.3.1高效能液相層析標準品的製備 23
2.8.3.2高效能液相層析樣品前處理步驟 23
2.8.3.3高效能液相層析儀設定參數 23
2.8.4化學需氧量(Chemical Oxygen Demand ;COD)測試實驗 24
三、結果 25
3.1菌株篩選結果 25
3.1.1固體篩選法 25
3.1.2液態篩選法 25
3.2菌種鑑定結果 26
3.3生長曲線測定 26
3.4生化特性分析結果 27
3.4.1 溫度生長範圍測試 27
3.4.2 酸鹼值生長範圍測試 27
3.5螢光增白劑分解測試實驗 28
3.5.1螢光強度測試結果 28
3.5.1.1檢量線 28
3.5.1.2 螢光強度測量結果 28
3.5.2薄層層析法分析結果 29
3.5.3高效能液相層析分析 29
3.5.3.1定性、定量及偵測極限 29
3.5.3.2檢量線製作 29
3.5.3.3回收率探討 30
3.5.3.4高效能液相層析結果 30
3.5.4化學需氧量測試 31
四、實驗討論 31
實驗之圖與表 37
參考文獻 96

步平譯、張貴民。(1998) 螢光增白劑的毒理學性質,日用化工業,第六期。

李江頌、王建行、李日強、王愛英。(2010) 螢光增白劑生產廢水不同與處理方法的比較.中國環境科學,第1459-1465頁。

邱永亮、魏盛德。(2000) 科學圖書大庫“染色化學(合) ’’,第182-188頁。

消費者報導。(2003) 消費者報導,第 267 期,第31-41 頁,民國92 年。

章一丹、徐灝龍、謝娟、等。(2007) 催化鐵內電解混凝生物膜法處理螢光增白劑生產廢水.江蘇環境科技,第20期,第33-34頁。

王健行、李日强。(2009) 螢光增白劑生產廢水的處理,水處理技術水處理技術,第11期,第35頁

王景國、容建明。(2002) 國內外螢光增白劑的狀況與展望,染料工業,第2期,第9頁
袁躍華、朱永軍、田茂忠。(2010) 螢光增白劑的應用及發展趨勢,山西大同大學學報,第26卷第5期

Abe, A., Yoshimi, H.(1979) Qualitative analysis of fluorescent whitening agents in river water.Water Res. 13, p.1111-1112.

Anliker, R. G., Anliker, M. R.(1975) History of fluorescent whitening agents. Environ. Qual. Saf. Suppl. 4, p.12-8.

Bauer, J. E., Kerr, R. P., Bautista, M. F.(1988) Effects of co-occuring aromatic hydrocarbons on degradation of in-dividual polycyclic aromatic hydrocarbonsin marine sedi -ment slurries. Appl. Environ. Microbiol. Mar. Environ. Res. 25, p.63- 84.

Bayer, A. G.(2000a) Determination of melting point(12/2000). Internal study.

Bayer, A. G.(2000b) Test on solubility in water(11/2000). Internal study.

Bayer A. G.(2000c) Acute daphnia toxicity. Internal study.

Bayer Industry Services(2004b) Determination of the water solubility(flask method) of blank ophor BBH content of sodium and potassium. Internal study.

Bayer Chemicals(2004) C.I. Fluorescent brightener 28/113 - internal data on production, processing, use pattern, and workplace exposure. Internal study.

Bennett, J. W., Bentley, R.(2000) Seeing red: the story of prodigiosin. Adv. Appl. Microbiol. 47, p. 1-32.

Bidlan, R., Manomani, H. K.(2002) Aerobic degradation of dichlorodiphenyltrichloroethane(DDT)by Serratia marcescens DT-1P. Process Biochem. 1, p.49-56.

Burckhardt, W., Burckhardt, K., Schwarz, S. M.(1957) Photoallergic eczemas caused by nadisan. Schweiz. Med. Wochenschr.28, p.954-956.

Burg, A. W., Rohovsky, M. W., Kensler, C. J., Wogan, G. N.(1977) Current status of human safety and environmental aspects of fluorescent whitening agents used in detergents in the United States. Crit. Rev. Env. Contr.7, p.91-120.

Chung, W. W., Bolte, M.(2001) 4,4-Diaminostilbene-2,20-disulfonate(DSD)
behavior : under irradiation in water.decrease of its activity as a fluorescent whitening agent. J. Photochem. Photobiol., A. 138, p.275–280.

Damant, A. P., Castle, L.(1999) Determination of fluorescent whitening agents in paper and board packaging materials by capillary electrophoresis. J. Microcol. Sep.11 .4, p.259-262.

Diba-Geigy Ltd.(1992a) Test No.: G 09803, Modified AFNOR Test. Internal Study.

Forbes, D. F.(1975) Photocarcinogenesis: lack of enhancement by fluorescent whitening agents in: fluorescent whitening agents. Environ. Qual. Saf. 4, p.212-222.

Ganz C. R., Liebert C., Schulze J. and Stensby P. S.(1975) Removal of detergent fluorescent whitening agents from wastewater. J. Wat. Pollut. Control Fed. 47, p.2834~2849.

Grimont, F., Grimont, P. A. D.(2006) The genus Serratia. Prokaryotes. 6, p.219-244.

Gugluelmetti, L.(1975) Photochemical and biological degradation of water-soluble FWAs. Environ. Qual. Saf. 4, p.180-190.

Gloxhuber, C. H.(1978) Bloching toxic logic properties of fluorescentwhitening agents. Clin. Toxicol. 13, p.196-197.

Han, W. Q., Wang, L. J., Sun, X. Y.(2008) Treatment of bactericide waste water by combined process chemical coagulation, electro-chemical oxidation and membrane bioreactor. J. Hazard. Mater. 151, p.306-315.

Hayashi, Y., Managaki, S., Takada, H.(2002) Fluorescent whiteningagents in tokyo bay and adjacent rivers: their application asanthropogenic molecular markers in coastal environments. EnvironSci. Technol. 36, p.3556-3563.

Jasperse, J. L., Steiger, P. H.(1992) A system for determining opticalbrighteners in laundry detergents by TLC and HPLC. J. Am.Oil Chem.Soc. 69, p.621-625.

Jokinen, O., Baak, R., Traser, G., Rohringer, P.(2000) Optical brighteners for high white coated papers. Wochenblattfur. Paperfabirkation. 128, p.590–593.

Katayama, M., Kurata, Y., Abe, S.(1982) Biodegradation of Fluorescent whitening Agents(FWAs) in waste water--disappearance by the activated sludge treatment. Nippon Kasei Gakkaishi. 33, p.124-128.

Keplinger, M. L., Lymann, F. L., Calandra, J. C.(1975) Chronic toxicity and carcinogenicity studies with FWAs in fluorescent whitening agents. Environ. Qual. Saf. Suppl. 1, p. 212 - 222.

Kim, J. S., Kim, D. H., Kim, K.(2012) Determination of fluorescent whitening agents in paper materials bull. Korean Chem. 33, p.12

Konstantinova, T., Konstantinova, H., Betcheva, R.(1999) The synthesis and properties of some unsaturated triazinylstilbene fluorescent brightening agents.Dyes Pigment. 43, p197-201.

Kuo, W. G.(1992) Decolorizing dye wastewater with Fenton`s reagent, Water Research. 26, p.881-886

Kramer, J. B., Canonica, S., Hoigé, J.(1996) Degradation of fluorescent whitening agents in sunlit natural waters. Environ. Sci. Technol. 30, p.2227-2234.

Kramer, J. B.(1992) Fluorescent whitening agents, in : the handbook of environmental chemistry, Vol. 3, Part F: Anthropogenic Compounds, Detergents(eds. Hutzinger, O., de Oude N. T.). Springer, p.351-366.

Lee, J. Y., Youn, H. J., Lee, H. L.(2012) Fundamental study for quantitative analysis of the fluorescent whitening agent content of paper and process water. BioResources. 7, p.315-326.

Lee, J. V., Shread, P., Furniss, A. L., Bryant, T. N.(1981) Taxonomy and description of Vibrio fluvialis sp. nov.. J. Appl. Microbiol. 50, p.73–94.

Li, M. T., Hao, L. L., Sheng, L. X., Xu, J. B.(2008) Identification and degradation characterization of hexachlorobutadiene degrading strain Serratia marcescens
HL1. Bioresour. Technol. 15, p.6870-6884.

Lyman, F. L. J., chulze, D. R., Ganz, P. S., Stensby, M. L., Keplinger, I. D.(1975) Long 2 term toxicity of four fluorescent whitening agents. Fd. Co. smet. Tox. 13, p.521- 527.

Micali, G., Curro, P., Calabro, G.(1984) High performance liquidchromatographic separation and determination of fluorescentwhitening agents in detergents. Analyst. 109, p.155-158.

Novartis Services, A. G.(1997) Test No.: N 03713, Modified Zahn-Wellens Test. Internal Study.

Paprowicz, J., Slodczyk, S.(1988) Application of Biologically Activated Sorptive
Columns for Textile Waste Water Treatment. Envir. Tec. Letters. 9, p.271-280

Poiger, T., Field, J. A., Field, T. M., Giger, W.(1993) Determination of detergent-derived fluorescent whiting agents in sewage sludges by liquid chromatography. Anal. Methods Instrum. 1, p.104-113.

Poiger, T., Field, J. A., Field, T. M., Giger, W.(1996) Occurrence of fluorescent whitening agents in sewage and river water determined by solid-phase extraction and high-performance liquid chromatography. Environ. Sci. Technol. 30, p.2220-2226.

Poiger, T., Field, J. A., Field, T. M., Siegrist, H., Giger, W.(1998) Behavior of fluorescent whitening agents during sewage treatment. Elsevier Science Ltd. 6, p.1939-1947.

Roick, T. K., Ocher M.(1997) Optical brighteners for fine papers. Das. Papier. 6, p.114–117.

Snaidr, J., Amann, R., Huber, I., Ludwig, W., Schleifer, K. H.(1997) Phylogenetic Analysis and In Situ Identification ofBacteria in Activated Sludge. Appl. Environ. Microl. 7, p.2884–2896.

Tsuji, K., Natito, S., Nakazawa, H.(1988) Determination of fluoresceent whitening agents in river water by highperformance liquid chromatography. Eisei. Kagaku. 34, p.501-507.

Veleva, S., Valacheva, E., Valchev, I., Radeva, G.(2001) Application of an exponential kinetic equation to the interaction of optical brighteners with pulp. Kinetics and Cataysis. 72, p.355-364.

Verma, P., Madamwar, D.(2003) Decolourization of synthetic dyes by a newly isolated strain of Serratia marcescens. World J. Microb. Biot. 6, p.615-618.

West, P. A., Okpokwasili, G. C., Brayton, P. R.(1984) Numerical taxonomy of phenanthrene - degrading bacteriaisolated from the Chesapeake Bay. Appl. Environ. Microl. 48, p.988- 993.

Yu, V. L.(1979) Serratia marcescens: historical perspective and clinical review. N. Engl. J. Med. 16, p. 887-893.

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