跳到主要內容

臺灣博碩士論文加值系統

(44.201.99.222) 您好!臺灣時間:2022/12/05 23:03
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:張智翔
研究生(外文):Chin-Shiang Chang
論文名稱:利用米麴菌氣舉式醱酵生產異黃酮多酚之研究
論文名稱(外文):The Study of Isoflavone Polyphenol by Asperillus oryzae Production in Airlift Fermenter
指導教授:吳聲祺
指導教授(外文):Sheng-Chi Wu
口試委員:張德生林雅菁
口試委員(外文):Te-Sheng ChangYa-Ching Lin
口試日期:2014-07-09
學位類別:碩士
校院名稱:輔英科技大學
系所名稱:生物科技系碩士班
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:42
中文關鍵詞:異黃酮Genistein氣舉式發酵
外文關鍵詞:isoflavoneGenisteinairlift fermenter
相關次數:
  • 被引用被引用:0
  • 點閱點閱:486
  • 評分評分:
  • 下載下載:17
  • 收藏至我的研究室書目清單書目收藏:0
Genistein是一種大豆異黃酮,並大量存在植物中。近年來已被研究在人體上,包含預防骨質疏鬆、降低心血管疾病及減緩癌症等功效。本實驗是利用Asperillus oryzae進行生物轉化,將Genistein轉化成8-Hydroxgenistein,探討培養基的組成與氣舉式醱酵槽的培養參數,以評估大量生產8-Hydroxgenistein的可行性。
Isoflavones are naturally occurring dietary phytoestrogens and present in various plants. Genistein is one of isoflavones. In recent years, isoflavones have been under intensive investigation due to their possible role in preventing certain hormone-dependent and other diseases, including prevention of osteoporosis, cardiovascular disease and mitigation to reduce cancer and other effects.
In this study, the production of 8-Hydroxygenistein using biotransformation by Asperillus oryzae was investigated. The effects of medium compositions and cultivation condition were discussed. The optimal medium compositions were obtained and the production conditions were examined in airlift fermenter.

誌謝 i
中文摘要 ii
英文摘要 iii
目錄 iv
表目錄 v
圖目錄 vi
符號說明 vii
第一章 緒論 1
第二章 文獻探討 2
第一節 固態發酵 2
(一) 固態發酵之簡介 2
(二) 澱粉水解酵素(Alpha-amylase) 3
第二節 大豆異黃酮簡介與應用 4
(一) 異黃酮簡介 4
(二) 異黃酮之生理功能應用 4
(三) 異黃酮多酚之生產與生理功能 5
第三章 實驗材料與方法 6
第一節 材料與設備 6
(一) 材料 6
(二) 設備 7
第二節 實驗方法 8
(一) 菌種保存 8
(二) 菌株冷凍保存 8
(三) 菌株活化8
(四) 接種之孢子液製備 8
(五) 生產孢子之固態培養方法 9
(六) 酵素活性分析9
(七) 搖瓶培養 10
(八) 樣品萃取 10
(九) 8-hydroxgenistein(8OHGe)定量分析 10
(十) HPLC分析8-hydoxygenistein之條件 11
第四章 結果與討論 12
第一節 利用固態培養方式生產孢子 12
第二節 探討生產8-Hydroxgenisten的最適化條件 13
第五章 討論與結論 16
參考文獻 17

Anto H., U. B. Trivedi and Patel K. C. (2006). "Glucoamylase production by solid-state fermentation using rice flake manufacturing waste products as substrate." Bioresour Technol 97(10): 1161-1166.

Chang, T. S., S. Y. Chai and Y. C. Chen (2013). "Production of ortho-hydroxydaidzein derivatives by a recombinant strain of Pichia pastoris harboring a cytochrome P450 fusion gene." Process Biochemistry 48(3): 426-429.

Chang, T. S., H. Y Ding., S. K. Sorgan and C. Y. Wu (2007). "Mushroom tyrosinase inhibitory effects of isoflavones isolated from soygerm koji fermented with Aspergillus oryzae BCRC 32288." Food Chemistry 105: 1430–1438.

Chang, T. S., H. Y. Ding, S. K Sorgan. and C. Y. Wu (2007). "Metabolism of the Soy Isoflavones Daidzein and Genistein by Fungi Used in the Preparation of Various Fermented Soybean Foods." Bioscience, Biotechnology, and Biochemistry 71(5): 1330-1333.

Chen Y. C., M. Inaba, N. Abe and A. Hirota ( 2003). "Antimutagenic activity of 8-hydroxyisoflavones and 6-hydroxydaidzein from soybean miso." Biosci Biotechnol Biochem. 67(4): 903-906.

Esaki H., H. Onozaki, M. Yasujiro, S. Kawakishi and T. Osawa (1998). "Potent Antioxidative Isoflavones Isolated from Soybeans Fermented with Aspergillus saitoi." Bioscience Biotechnology and Biochemistry 62(4): 740-746.

Esaki H., H. Onozaki, M. Yasujiro, S. Kawakishi and T. Osawa (1999). "Formation Mechanism for Potent Antioxidative o-Dihydroxyisoflavones in Soybeans Fermented with Aspergillus saitoi." Biosci. Biotechnol. Biochem.. 63( 5): 851-858S858.

Francis, F., A. Sabu, K. M. Nampoothiri, S. Ramachandran, S. Ghosh, G. Szakacs and A. Pandey (2003). "Use of response surface methodology for optimizing process parameters for the production of α-amylase by Aspergillus oryzae." Biochemical Engineering Journal 15(2): 107-115.

Fujita T., T. Funako and H. Hayashi (2004). "8-Hydroxydaidzein, an aldose reductase inhibitor from okara fermented with Aspergillus sp. HK-388." Biosci Biotechnol Biochem 68(7): 1588-1590.

Funayama S., Y. Anraku, A. Mita, K. Komiyama and S. Omura (1989 ). "Structural study of isoflavonoids possessing antioxidant activity isolated from the fermentation broth of Streptomyces sp." J Antibiot (Tokyo). 42(9): 1350-1355.

Hirota A., i S. Tak, S. Kawaii, M. Yano and N. Abe (2000). "1,1-Diphenyl-2-picrylhydrazyl radical-scavenging compounds from soybean miso and antiproliferative activity of isoflavones from soybean miso toward the cancer cell lines." Biosci Biotechnol Biochem. 64(5): 1038-1040.

Jung S., P. A. Murphy and I. Sala (2008). "Isoflavone profiles of soymilk as affected by high-pressure treatments of soymilk and soybeans." Food Chemistry 111(3): 592-598.

Kashyapa P., A. Sabua, A. Pandeya, G. Szakacs and C. R. Socool (2002). "Extra-cellular L-glutaminase production by Zygosaccharomyces rouxii under solid-state fermentation." Process Biochemistry 38: 307-312.

Klus, K. and Barz W. (1995). "Formation of polyhydroxylated isoflavones from the soybean seed isoflavones daidzein and glycitein by bacteria isolated from tempe." Arch Microbiol 164(6): 428-434.

Sotti L., M. J. FJ. Bezerra, M. Maqalhaes, M. Silva , IR. Pitta, MT.Scotti (2012). "SAR, QSAR and Docking of Anticancer Flavonoids and Variants: A Review." Current Topics in Medicinal Chemistry 12(24): 2785-2809.

Lo Y. L. (2012). "A Potential Daidzein Derivative Enhances Cytotoxicity of Epirubicin on Human Colon Adenocarcinoma Caco-2 Cells." Int. J 14(1): 158-176.

Pandey A. (2003). "sodium-state fermentation." Biochemical Engineering Journal 13: 81–84.

Punjaisee C., C. Chaiyasut, S. Chansakaow, S. Tharata, W. Visessanguan and S. Punjaisee (2011). "8-Hydroxygenistein formation of soybean fermented with Aspergillus oryzae BCC 3088." 6(4): 785-789.

Pyo, Y. H., T. C. Lee and Y. C. Lee (2005). "Effect of Lactic Acid Fermentation on Enrichment of Antioxidant Properties and Bioactive Isoflavones in Soybean." Journal of Food Science 70(3): S215-S220.

Raj Kashyap D., S. Kumar Soni and R. Tewari (2003). "Enhanced production of pectinase by Bacillus sp. DT7 using solid state fermentation." Bioresource Technology 88(3): 251-254.

Ramachandran S., K. Roopesh, K. M. Nampoothiri, G. Szakacs and A. Pandey (2005). "Mixed substrate fermentation for the production of phytase by Rhizopus spp. using oilcakes as substrates." Process Biochemistry 40(5): 1749-1754.

Kapiotis S., M. Hermann, I. Held, C. Seelos, H. Ehrinqer, B.M. Gmeiner (1997). "Genistein, the Dietary-Derived Angiogenesis Inhibitor, Prevents LDL Oxidation and Protects Endothelial Cells From Damage by Atherogenic LDL." Arterioscler Thromb Vasc Biol. 11(26): 68-74.

Boue S. M., F. F. Shin, B.Y. Shin, K.W. Daigle, C.H. Carter-Wientjes and T.E. Cleveland ( 2008). "Effect of biotic elicitors on enrichment of antioxidant properties and induced isoflavones in soybean." J Food Sci 73(4): H43-49.

Seo M. H., B. N. Kim, K. R. Kim, K. W. Lee, C. H. Lee and D. K. Oh (2013). "Production of 8-hydroxydaidzein from soybean extract by Aspergillus oryzae KACC 40247." Bioscience, Biotechnology, and Biochemistry 77(6): 1245-1250.
Silva D., K. Tokuioshi, E. da Silva Martins, R. Da Silva and E. Gomes (2005). "Production of pectinase by solid-state fermentation with Penicillium viridicatum RFC3." Process Biochemistry 40(8): 2885-2889.

Soccol C. O., B. Marin , M. Raimbault , -M. Lebeault (1994). "Potential of solid state fermentation for production of L(+)-lactic acid by Rhizopus oryzae." Applied Microbiology and Biotechnology 41(3): 286-290.

Valladares L., A. Garrido and W. Sierralta (2012). "Soy isofl avones and human health: breast cancer and puberty timing." Revista medica de Chile 140: 512-516.

Soares V. F., L. R. Castitho., E. P. Bon and D. M. Freire (2005). "High-yield Bacillus subtilis protease production by solid-state fermentation."Appl Biochem Biotechnol 121: 311.

Xia L. C., P. (1999). "Cellulase production by solid state fermentation on lignocellulosic waste from the xylose industry." Process Biochemistry 34: 909-912.

Yamaguchi* M. (2002). "Isoflavone and Bone Metabolism: Its Cellular Mechanism and Preventive Role in Bone Loss." Journal of Health Science 48(3): 209–222.

李林輝 (2004). "固態培養(發酵)裝置的類型、應用及問題,." 西華師範大學學報。.

徐軍, 崔麗霞 and 韓建榮 (2004). "利用穀物進行類胡蘿蔔素固態發酵." 食品與發酵工業 30 5-8.

楊嘉凌 (2004). "α -amylase 在禾穀類種子中扮演的角色." 台中區農業改良場八十九年度試驗研究暨推廣學術研討會報告摘要 49: 1.

鄭秀卿 (2005). "以動物模式建立營養素或藥品影響骨骼發育的評估方法." 中原大學化學系

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊