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研究生(外文):Chin-Shiang Chang
論文名稱(外文):The Study of Isoflavone Polyphenol by Asperillus oryzae Production in Airlift Fermenter
指導教授(外文):Sheng-Chi Wu
口試委員(外文):Te-Sheng ChangYa-Ching Lin
外文關鍵詞:isoflavoneGenisteinairlift fermenter
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Genistein是一種大豆異黃酮,並大量存在植物中。近年來已被研究在人體上,包含預防骨質疏鬆、降低心血管疾病及減緩癌症等功效。本實驗是利用Asperillus oryzae進行生物轉化,將Genistein轉化成8-Hydroxgenistein,探討培養基的組成與氣舉式醱酵槽的培養參數,以評估大量生產8-Hydroxgenistein的可行性。
Isoflavones are naturally occurring dietary phytoestrogens and present in various plants. Genistein is one of isoflavones. In recent years, isoflavones have been under intensive investigation due to their possible role in preventing certain hormone-dependent and other diseases, including prevention of osteoporosis, cardiovascular disease and mitigation to reduce cancer and other effects.
In this study, the production of 8-Hydroxygenistein using biotransformation by Asperillus oryzae was investigated. The effects of medium compositions and cultivation condition were discussed. The optimal medium compositions were obtained and the production conditions were examined in airlift fermenter.

誌謝 i
中文摘要 ii
英文摘要 iii
目錄 iv
表目錄 v
圖目錄 vi
符號說明 vii
第一章 緒論 1
第二章 文獻探討 2
第一節 固態發酵 2
(一) 固態發酵之簡介 2
(二) 澱粉水解酵素(Alpha-amylase) 3
第二節 大豆異黃酮簡介與應用 4
(一) 異黃酮簡介 4
(二) 異黃酮之生理功能應用 4
(三) 異黃酮多酚之生產與生理功能 5
第三章 實驗材料與方法 6
第一節 材料與設備 6
(一) 材料 6
(二) 設備 7
第二節 實驗方法 8
(一) 菌種保存 8
(二) 菌株冷凍保存 8
(三) 菌株活化8
(四) 接種之孢子液製備 8
(五) 生產孢子之固態培養方法 9
(六) 酵素活性分析9
(七) 搖瓶培養 10
(八) 樣品萃取 10
(九) 8-hydroxgenistein(8OHGe)定量分析 10
(十) HPLC分析8-hydoxygenistein之條件 11
第四章 結果與討論 12
第一節 利用固態培養方式生產孢子 12
第二節 探討生產8-Hydroxgenisten的最適化條件 13
第五章 討論與結論 16
參考文獻 17

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