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研究生:林俊宏
研究生(外文):Jyun-Hong Lin
論文名稱:以動物模式探討創傷壓力前後對神經內分泌之影響
論文名稱(外文):Neuronendocrine Alteration before and after Traumatic Stress in an Animal Model
指導教授:藍心婕
指導教授(外文):Hsin-Chieh Lan
口試委員:徐佳福王家儀
口試委員(外文):Jia-Fwu ShyuJia-Yi Wang
口試日期:2014-05-23
學位類別:碩士
校院名稱:國防醫學院
系所名稱:生物及解剖學研究所
學門:生命科學學門
學類:生物訊息學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:65
中文關鍵詞:創傷後壓力症候群
外文關鍵詞:PTSD
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創傷後壓力症候群 (PTSD)是一種複雜的症狀,其定義是個體曾有暴露於恐懼以及危及生命的創傷經驗,之後可能導致生理及心理的異常。PTSD的患者可能會每天有重複體驗以及非常警覺的情形。之後將進一步出現一些精神症狀,包括持續焦慮、過度驚嚇、認知功能障礙和較不能抵抗恐懼以及皮質素(cortisol)會異常的低。要研究PTSD是相當困難的,因為PTSD的發展是相當多樣且複雜的,而且壓力之後的症狀在不同的時期也是非常多變的。個體之間的生理差異與不同的生活經歷對於此疾病也是重要的影響因素。因此,我們利用動物模式進行實驗,希望找出創傷壓力對內分泌系統的影響。
我們將八周大的SD大鼠暴露於空間緊迫壓力或天敵壓力,並透過尾靜脈採取刺激前後的血液樣本進行荷爾蒙分析 (皮質素和雌激素)。我們也利用行為測試 (開放空間測試和懸尾測試)以檢測動物是否有類似PTSD的症狀。三個月之後,我們犧牲所有大鼠並取下組織樣本 (腦、睪丸、卵巢、腎上腺)。
在我們的結果中,有10%的動物在生存壓力之後確實出現類似PTSD症狀的行為,他們表現出明顯的焦慮 (anxiety)及絕望 (despair)。而荷爾蒙檢測的結果中顯示,在壓力之後,類似PTSD症狀之大鼠之雌激素含量,比起控制組和有壓力無症狀的組別,來得更高。此外,有類似PTSD症狀大鼠之皮質酮,比起控制組和有壓力無症狀組,出現更低的趨勢。最後在分析關聯性的結果中,我們也觀察到公鼠之雌激素數值與兩種行為測試確實有關連性,顯示創傷壓力所造成的焦慮與絕望與動物體內的荷爾蒙有關。

Post-traumatic stress disorder (PTSD) is a complex syndrome that is defined by individual exposed to intense, life-threatening trauma leading to various physical and psychological abnormalities. The re-experiencing and avoidance symptoms of the disorder may hinder daily life in PTSD patients. They further develop additional debilitating symptoms, including persistent anxiety, exaggerated startle, cognitive impairments and diminished extinction of conditioned fear, and abnormally low cortisol levels. It is hard to study PTSD in human because the causes of PTSD are diverse and complicated and the symptoms are various in different periods after stress. Different life experience of individual is also an important factor in this disease. Therefore, we would like to use the animal model to approach the stress effects on endocrine systems.
We used eight-week-old SD rats to impose survival pressure of space urgency. Then collect blood samples from tail vein for hormone analysis (corticosterone and estrogen) before and after the stimulations. We also used the behavioral tests (open field test and tail suspension test) to determine whether the animal have the PTSD-like symptoms . Three months later, we sacrificed all rats and collect the tissue samples (brain, testis, ovary and adrenal) for further study in the future.
In our results, 10% of total rats were affected with survival pressure based on the behavioral tests and grouped as PTSD-like. These rats showed significant anxiety and depression after stress. In PTSD-like rats, estradiol levels are higher than control and stressed without symptom rats after stress. In corticosterone, PTSD-like rats show lower than control and stressed without symptom rats. We also observed that the estradiol level have some correlation with the results of behavioral tests in male rats. It indicates anxiety and depression induced by survival stress may be correlated with hormone secretions.

中文摘要-----------------------------------------------------------3
Abstract----------------------------------------------------------4
第一章、前言--------------------------------------------------------5
第二章、材料方法----------------------------------------------------13
第三章、結果--------------------------------------------------------19
第四章、討論-------------------------------------------------------27
第五章、參考文獻----------------------------------------------------33
第六章、圖表-------------------------------------------------------36

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