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研究生:李雅晶
論文名稱:化妝保養品添加鱸魚魚鱗萃取膠原蛋白之安定性研究
指導教授:王惠亮
學位類別:碩士
校院名稱:國立高雄師範大學
系所名稱:生物科技系
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:81
中文關鍵詞:金目鱸魚魚鱗膠原蛋白化妝品
外文關鍵詞:Lates calcariferFish scales CollagenCosmetic
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中文摘要
本研究探討金目鱸魚(Lates calcarifer)魚鱗之膠原蛋白萃取方式;將魚鱗經0.3 M NaOH除去皮膜後,再將去皮膜後的魚鱗以0.3 M HCl去灰份,再分別以0、0.5、1、2 M濃度的醋酸溶液在相同水域條件下同時萃取每管40 ml含有2 g的魚鱗,在4、8、12、16、20、24小時的時間點個別取出,再以Coomassie brilliant blue G-250測定膠原蛋白的含量,以2 M醋酸溶液100oC萃取20小時所得到的膠原蛋白含量最高,為678 μg/ml,發現萃取時間與醋酸濃度都會影響膠原蛋白的溶解量,時間越長、醋酸濃度越高越能有效的將膠原蛋白大分子分解成小分子胺基酸,但萃取時間在第16小時至20小時之間,萃取濃度為最高濃度。經滅菌及冷凍乾燥後,將得到的膠原蛋白粉末添加至化妝水、精華液、精華乳、營養霜中測試其產品穩定性及耐受性,在化妝水及營養霜的產品穩定性及耐受性測試均無析出垮體、分層、出油等現象,但精華液配方在室溫放置2週後3%的開始有析出物,放置3週後析出物開始結晶,連同2%的也發現有結晶。在離心機分離試驗膠原蛋白2%精華乳及膠原蛋白3%精華乳經離心後有分層微垮體現像。將4種不同膠原蛋白濃度化妝品成品以Coomassie brilliant blue G-250測定膠原蛋白的含量,由數據得知添加膠原蛋白濃度越高測得之膠原蛋白濃度會越高,鱸魚魚鱗可萃取出豐富天然之膠原蛋白,可做為化妝品天然保濕原料來源。

關鍵字:金目鱸魚、魚鱗、膠原蛋白、化妝品。

Abstract
This study was to investigate the collagen extraction methods and it application from the scale of Lates Calcarifer. First scale epithelium was to remove by 0.3 M NaOH, and then 0.3 M HCl was to use to obtain the deash of the fish scale. Each 40 ml tube containing 2 g of fish scale under the same water condition of 0, 0.5, 1 and 2 M concentration of acetic acid solution were extracted. After every 4, 8, 12, 16, 20, 24 hour time point, The Coomassie brilliant blue G-250 was used to determinate the concentration of the collagen for each sample. With 2 M acetic acid solution 100oC water extraction for 20 hours was obtained the maximum collagen concentration of 678 μg / ml. The result showed that the water extraction time and acetic acid concentrations can affect the dissolution amount of collagen in addition, the longer the time, and the higher the concentration acetic acid were effectively macromolecules degraded the collagen into smaller molecules amino acid. Extraction times were from 0 to 24 hours, the highest concentration of extract time was between 16 and 20. After sterilization and freeze-dried process, the different percentage of collagen powder (1%, 2% and 3%) was added to cosmetic formula, including toner, essence, lotion, and cream, and then test their stability and resistance. There were no precipitate collapse, stratification and oil phenomenon in toner and cream, but only the lotion formula with 3% collagen powder presented the started precipitates after placed in room temperature for two weeks. Precipitate started to crystalize after placing three weeks, as well as the 2% one. Resistance test in circulation oven and ultra-low temperature test both showed no sign of precipitate, collapse base, stratification and oil phenomenon. In addition, essence containg 2% and 3% of collagen powder appeared layered micro-collapse after the centrifuge seperation test. The four different concentrations of collagen cosmetic tested with Coomassie brilliant blue G-250 showed that the higher concentration of collagen added in cosmetic was measured also higher. The striped bass fish scale are rich in collagen that can be used as a natural moisturizing cosmetic raw material sources.

Keywords: Lates calcarifer , Fish scales Collagen, Cosmetic.


目 錄
中文摘要..........................................................................................................I
ABSTRACT...................................................................................................II
致謝...............................................................................................................IV
壹、前言...........................................................................................................1
一、台灣鱸魚概況.......................................................................................1
二、金目鱸魚介紹.......................................................................................1
三、魚鱗介紹...............................................................................................2
四、膠原蛋白...............................................................................................3
五、皮膚介紹...............................................................................................9
六、化妝品劑型定義及目的.....................................................................11
七、研究目的及預期成果.........................................................................11
貳、材料與方法............................................................................................13
一、材料....................................................................................................13
(一)鱸魚品種.........................................................................................13
(二)實驗藥品.........................................................................................13
(三)藥品配置.........................................................................................13
(四)化妝品原料.....................................................................................13
二、方法.....................................................................................................14
(一)魚鱗粗膠原蛋白之前處理.............................................................14
(二)氫氧基磷灰石製備.........................................................................14
(三)魚鱗膠原蛋白之萃取條件.............................................................15
(四)蛋白質濃度測定Bio-Rad protein Assay : Bradford......................15
(五)膠原蛋白粉末原料製備.................................................................15
(六)膠原蛋白水解成胺基酸含量測定.................................................16
(七)化妝品劑型應用.............................................................................16
(八)化妝品檢測項目.............................................................................21
(九)儀器設備.........................................................................................23
叁、結果.........................................................................................................24
一、鱸魚魚鱗前處理後粗膠原蛋白之產率.............................................24
二、氫氧基磷灰石之產率.........................................................................24
三、鱸魚魚鱗膠原蛋白萃取結果.............................................................24
四、膠原蛋白粉末原料1-3% 濃度測定結果...........................................25
五、膠原蛋白水解成胺基酸含量測定結果.............................................26
六、化妝品成品與評估結果.....................................................................26
七、化妝品檢測結果.................................................................................28
八、化妝品耐受性測試結果.....................................................................29
肆、討論.........................................................................................................31
伍、參考文獻.................................................................................................35
陸、圖表.........................................................................................................40
柒、附錄.........................................................................................................76


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