臺灣博碩士論文加值系統

本研究目的是以鹽酸、磷酸水解萃取米糠中之阿魏酸寡醣，並探討其抗氧化之活性，首先實驗水解米糠酸濃度和反應時間之範圍，分為：鹽酸濃度0.05、0.1、0.2、0.3 M，反應時間30、60、90分鐘；磷酸為0.25、0.5、1 M，反應時間60、90、120分鐘，將數據範圍及結果進行回歸分析得出回歸方程式，再以一次微分分析得出酸水解之最適條件並進行驗證試驗。結果顯示利用鹽酸、磷酸水解萃取米糠中之阿魏酸寡醣其最適條件為：鹽酸為0.144 M、反應62分鐘，而磷酸則為0.64 M、反應72分鐘。
另外，以鹽酸、磷酸最適條件大量水解萃取後透過分離純化所得之米糠阿魏酸寡醣，在Trolox當量的抗氧化能力 (TEAC) 當中未經中和處理的鹽酸水解樣品(阿魏酸寡醣)，經稀釋後濃度各為100、200、300 μg/ml進行抗氧化能力測定，分別得到7.2、11.6、44.5 trolox eq. μg/g，經過中和處理之鹽酸水解樣品(阿魏酸)是3.5、6.9、22.4 trolox eq. μg /g；另外，磷酸水解米糠阿魏酸寡醣及阿魏酸兩種樣品分別為阿魏酸寡醣是6.7、12.2、33.7 trolox eq. μg /g，而阿魏酸則是5.4、9.6、23.9 trolox eq. μg /g，兩種不同酸水解所得之樣品皆具有活性，但阿魏酸寡醣樣品所得之抗氧化能力較阿魏酸樣品高，推測為可能樣品經過
中和後造成阿魏酸寡醣氫離子的解離，降低了樣品的抗氧化活性，故得結果為連接著寡醣的阿魏酸其抗氧化活性高於阿魏酸。

This study was to investigate the isolation conditions of feruloylated oligosaccharides from rice bran by using hydrochloric acid, phosphoric acid hydrolysis and the antioxidant activity of feruloylated oligosaccharides.
First, tested the range of hygrolyzed rice bran and reaction time the following were: Hydrochloric acid (HCl) concentration- 0.05, 0.1, 0.2, 0.3M; reaction time- 30, 60, 90 minute.Phosphoric acid (H3PO4) concentration- 0.25, 0.5, 1M; reaction time- 60, 90, 120 minute.
The result of conditions scope were calculated by regression equation, then obtained a defferential analysis of the optimal conditions for acid hydrolysis and validation tests. The result showed that the optimal conditions of hydrolysis rice bran for hydrochloric acid, phosphoric acid were:
Hydrochloric acid (HCl) concentration- 0.144M; reaction time- 62 minute.
Phosphoric acid (H3PO4) concentration- 0.64M; reaction time- 72 minute.
Furthermore, after hydrolysis rice bran by the optimal conditions of hydrochloric acid and phosphoric acid. The feruloylated oligosaccharides of rice bran was separated and purified from the extract. In the activity of Trolox equivalent antioxidant that the sample of feruloylated oligosaccharides from rice bran by using HCl hydrolysis without neutralization. Concentration of the sample were diluted to 100, 200, 300 μg / ml. The result were 7.2, 11.6, 44.5 trolox eq. μg / g. Activity for the sample of feruloylated oligosaccharides from rice bran by using HCl hydrolysis with neutralization were 3.5, 6.9, 22.4 trolox eq. μg / g.
Then, activity of Trolox equivalent antioxidant that the sample of feruloylated oligosaccharides from rice bran by using H3PO4 hydrolysis without neutralization. Concentration of the sample were diluted to 100, 200, 300 μg / ml. The result were 6.7, 12.2, 33.7 trolox eq. μg / g. Activity for the sample of feruloylated oligosaccharides from rice bran by using H3PO4 hydrolysis with neutralization were 5.4, 9.6, 23.9 trolox eq. μg / g.
Both of the sample by using hydrochloric acid, phosphoric acid hydrolysis that have activity of Trolox equivalent antioxidant, but the sample without neutralization were higher than the sample with neutralization. The situation might due to the sample after neutralization cause by ion of feruloylated oligosaccharides were dissociation, thereby affecting the antioxidant activity.
X
The result that ferulic acid connected oligosahharides with higher antioxidant activity.

謝誌 ............................................................................................................. I
目次 ............................................................................................................ II
頁次 ............................................................................................................ II
圖目錄 ...................................................................................................... IV
表目錄 ...................................................................................................... VI
中文摘要 ................................................................................................. VII
英文摘要 .................................................................................................. IX
前言 ............................................................................................................. 1
文獻回顧 ..................................................................................................... 3
ㄧ、米糠的化學組成 .......................................................................... 6
二、米糠中具機能性之物質 ........................................................... 10
三、阿魏酸 ........................................................................................ 14
四、阿魏酸寡醣(Feruloylated oligosaccharide; FO) ....................... 19
参、材料與方法 ...................................................................................... 22
一、實驗設計 .................................................................................... 22
二、實驗材料 .................................................................................... 23
2.1材料： ................................................................................... 23
III
2.2儀器設備： ........................................................................... 23
三、實驗方法 .................................................................................... 24
3.1酸水解 ................................................................................... 24
3.2阿魏酸醯基定量 ................................................................... 24
3.3最適條件分析 ....................................................................... 25
3.4阿魏酸寡醣純化分離 ........................................................... 25
3.5 Trolox 當量的抗氧化能力測定(TEAC) ............................. 25
肆、結果與討論 ...................................................................................... 26
一、酸水解米糠萃取之阿魏酸寡醣含量 ....................................... 26
二、鹽酸水解米糠最適條件 ........................................................... 29
三、磷酸水解米糠最適條件 ........................................................... 35
四、最適條件分析 ............................................................................ 39
伍、結論 ................................................................................................... 46
陸、參考文獻........................................................................................... 47

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