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研究生:卓奕均
研究生(外文):Cho, I-Chun
論文名稱:回散射式離子束細胞照射系統開發
論文名稱(外文):Development of a backscattering charged particle cell irradiation system
指導教授:許靖涵牛寰
指導教授(外文):Hsu, Ching-HanNiu, Huan
學位類別:博士
校院名稱:國立清華大學
系所名稱:生醫工程與環境科學系
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:英文
論文頁數:87
中文關鍵詞:離子束輻射生物效應加速器
外文關鍵詞:Ion beamRadiobiological effectAccelerator
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隨著離子癌症治療技術的發展及長途太空旅行計畫之實現,對於離子射束誘發之細胞輻射生物效應之了解亦日趨重要。本計畫之目標為於國立清華大學加速器實驗室3MV范氏加速器射束線上,發展一套結構簡單、安裝容易以及成本低廉之離子束細胞照射系統。於此系統設計中,我們運用了回散射技術來取代於其他離子束細胞照射系統中常見的 90∘偏轉磁鐵。此項創新使NTHU離子束細胞照射系統得以大幅簡化照射系統之設計同時有效降低安裝系統所需的資金需求。同時於此計畫中,我們藉由氮化矽薄膜於真空封膜及細胞培養皿基底之應用,有效的降低離子束照射過程中細胞樣品內之離子束能量分散。基於系統測試之結果,我們可以證實 NTHU 離子束細胞照射系統可提供一射束直徑為微米或次微米等級的均勻垂直方向離子束以用於細胞或次細胞照射。
As the development of the charged particle cancer treatment and the accomplishment long-term human space travel, the knowledge of the charged particle induced cellular biological effect is getting more and more important. The goal of this work is to develop a simple construction, easy installation and cost-effective charged particle cell irradiation system on the beam line of the 3 MV KN Van de Graaff accelerator in the Accelerator Laboratory of National Tsing Hua University (NTHU). In the system design, we applied the backscattering technique to instead of the usage of 90° bending magnet, which widely used for beam direction bending in other systems. This improvement is largely simplifying the system design as well as massively decreases the budget requirement in system installation. Moreover, the applications of silicon nitride membrane to the vacuum seal membrane and basement of cell culture dish in this project effectively eliminate the particle energy dispersion within a cell target. Based on the system performance test results, we confirm that NTHU charged particle cell irradiation system can provide a uniform vertical charged particle beam to execute the cellular or subcellular charged particle irradiation.
Abstract..................................................I
摘要......................................................II
List of Figures...........................................V
List of Tables.........................................VIII
Chapter 1 Introduction and Overview.......................1
Chapter 2 Background......................................3
2.1 System design requirements............................4
2.2 Microbeam radiobiological applications...............11
2.3 Radiation-induced DNA damage and repair..............13
Chapter 3 NTHU Cell Irradiation System...................18
3.1 Van de Graaff accelerator............................18
3.2 Main scattering chamber..............................22
3.3 Silicon nitride membrane.............................29
3.4 Beam formation Micro-pinhole.........................32
3.5 Cell culture supplements.............................36
3.6 Automatic irradiation control programs...............39
3.7 γ-H2AX immunofluorescence staining...................41
3.8 Image analysis macros................................44
Chapter 4 System performance test........................51
Chapter 5 Cell irradiation test..........................58
5.1 Giant cell formation test............................59
5.2 Charged particle induced γ-H2AX foci formation test..60
5.3 Cancer stem cell irradiation test....................66
Chapter 6 Conclusion.....................................70
Appendix A. The procedure of silicon nitride membrane production...............................................73
Appendix B. The procedure of Micro-pinhole collimator production...............................................74
Appendix C. Immunofluorescence staining procedure........76
Appendix D. Image-J macro of Make DAPI image ROI.........77
Appendix E. Image-J macro of Foci counting...............78
Reference................................................79


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