本篇研究從宜蘭大溪漁港中的海水，分離出三株具脂肪分解活性的 Alteromonas species D3、D5 和 D8，其中 Alteromonas D5 所產的脂肪分解&;#37238;具有熱穩定性及鹼穩定性，並經由硫酸銨沉澱及陰離子交換層析將酵素部分純化，最終酵素產量為0.11 %，純化倍率為 15.6。螢光活性呈色顯現出 5 個條帶具有活性，SDS-PAGE 顯示出含量最多的酵素分子量約為 30 kDa，但最高活性的酵素分子量約為4 0 kDa。酵素活性最好的條件為 70℃ 及 pH 8，在 20-80 ℃ 及 pH 6-9 都具有活性；多種金屬離子、抑制劑及界面活性劑的測試用來評估酵素的特性；經由實驗觀察，酵素能催化約 90 % 的棕櫚酸與甲醇反應並轉換成酯類，且酵素與四種市售清潔劑混合處理後能維持 80 % 以上的活性。根據本篇研究，此脂肪分解&;#37238;具有工業應用的潛力。

中文摘要 v
Abstract vi
第一章 前言 1
第一節、細菌所產脂肪分解&;#37238;之分類 1
第二節、脂肪分解&;#37238;的應用 2
第三節、從微生物取得脂肪分解&;#37238;之優點 4
第四節、研究動機 6
第二章 材料和方法 7
第一節、實驗材料與設備 7
第二節、實驗所使用之培養基 9
第三節、細菌樣本 10
第四節、分離、培養與篩選菌株 10
第五節、菌株之分泌脂肪分解&;#37238;檢測 10
第六節、蛋白質定量 11
第七節、脂肪分解&;#37238;活性測試 11
第八節、分離株形態與生理生化測試 12
第九節、分離株之 16S rRNA 序列分析 17
第十節、脂肪分解&;#37238;的純化 19
第十一節、脂肪分解&;#37238;的生化特性分析 22
第十二節、脂肪分解&;#37238;的應用 25
第十三節、統計分析 27
第三章 結果 28
第一節、菌株分離與細菌脂肪分解&;#37238;的活性測試 28
第二節、分離株之脂肪分解&;#37238;活性大小 28
第三節、分離株形態與生理生化特性 29
第四節、分離株之 16S rRNA基因序列分析 30
第五節、分離株之 D5 培養條件測試 30
第六節、脂肪分解&;#37238;的純化 31
第七節、脂肪分解&;#37238;的生化特性分析 32
第八節、脂肪分解&;#37238;的應用 34
第四章 討論 36
第一節、海洋細菌Alteromonas species 分離株的鑑定 36
第二節、脂肪分解&;#37238;的純化和特性探討 37
第三節、脂肪分解&;#37238;的應用 40
第五章 結論 42
參考資料 43

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