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研究生:駱君樺
研究生(外文):Chun-Hua Lo
論文名稱:2012年臺灣馬匹馬傳染性貧血之血清盛行率調查
論文名稱(外文):Seroprevalence of Equine Infectious Anemia in Horses of Taiwan in 2012
指導教授:吳應寧
指導教授(外文):Ying-Ling Wu
口試委員:季昭華林辰栖
口試委員(外文):Chau-Hwa ChiChen-Si Lin
口試日期:2014-04-10
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:臨床動物醫學研究所
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:50
中文關鍵詞:臺灣流行病學傳染性貧血血清盛行率
外文關鍵詞:TaiwanEpidemiologyHorseEquine infectious anemiaSeroprevalence
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馬傳染性貧血(EIA)是由反轉錄病毒科(Retroviridae)慢病毒亞科(Lentivirinae)之病毒所引起的馬科動物傳染病。受感染的馬匹會終身帶原,因此血清學檢驗結果為陽性的馬匹就認定為已受感染。此病為OIE listed disease,典型臨床症狀會出現高熱、貧血、體重喪失。於懷孕母馬可能會造成流產,且病毒也能經由胎盤傳染給胎兒。大多數感染此病的馬匹可能呈現無臨床症狀或臨床症狀不明顯的隱性帶原或慢性感染狀態。但都有可能會反覆的發生嚴重臨床症狀甚至導致死亡。此病自2001年在台灣進行EIA的調查後,10年來台灣地區未曾再進行過相關調查。因此本研究將針對台灣地區馬匹EIA進行調查,以期彌補十年來相關研究不足之憾。本實驗中所採集的217個血清樣本中,分別為臺灣北部(台北、桃園、新竹)121匹、中部(苗栗、台中)41匹、南部(高雄、屏東)35匹和東部(花蓮、台東)20匹,採集全臺馬場共23場。以酵素免疫分析法(Enzyme-linked immunosorbent assay,ELISA)檢測血清樣本之EIA抗體,217匹皆為陰性反應。自1992年的調查報告全台有2.1%的血清陽性率後,已連續20年調查無陽性結果。可知我國在馬傳染性貧血檢疫上極成功,但未來還是需要持續的監控與嚴格檢疫才能保持成果。

The Equine infectious anemia (EIA) is an infectious disease caused by the Equine infectious anemia virus, a lentivirus belonging to the Retroviridae family. EIA is an OIE listed disease. Infected horses will become carriers for life; therefore any horse with a positive result of serological test will be identified as infected. It’s typical clinical signs include high fever, anemia, and weight loss. Abortion may occur if pregnant mares are infected, and the virus can infect the fetus via the placenta. Most horses are infected with EIA with no obvious clinical signs as latent carriers, or in chronic stage with mild clinical signs. Both of latent carriers and chronically infected horses can show repeated severe clinical signs, even ending up with death. EIA serological test is necessary in the import and export of horses. There has been no investigation of EIA in Taiwan in the past ten years ever since 2001. In this study we took all 217 blood samples from the northern region (Taipei and Taoyuan, Hsinchu), the central region (Miaoli, and Taichung), the southern region (Kaohsiung and Pingtung), and the eastern region (Hualien and Taitung). Samples were tested by enzyme-linked immunosorbent assay (ELISA), and there is no positive result. It’s been 20 years we have no positive result in Taiwan since 1992. It can be seen as a huge success of our EIA elimination strategies, but still need to continuously investigate in the future.

口試委員會審定書 #
誌謝 i
中文摘要 ii
ABSTRACT iii
CONTENTS iv
LIST OF TABLES vi
Chapter 1 Introduction 1
Chapter 2 Literature Review 3
2.1 Etiology 3
2.2 Epidemiology 3
2.3 Clinical Signs 5
2.4 Diagnosis 6
2.5 Treatment 7
2.6 Prevention 7
Chapter 3 Material and Methods 9
3.1 Sample Size and Selection of Horses 9
3.2 Blood Samples 10
3.3 Putative Risk Factors 10
3.4 Equine Influenza ELISA 10
3.4.1 General Information 10
3.4.2 Descriptions and Principle 11
3.4.3 Instruments 11
3.4.4 Preparation of Sample and Wash Solution 12
3.4.5 Procedure of ELISA 12
3.5 Statistical Analyses 13
Chapter 4 Results 14
Chapter 5 Discussion and Conclusion 16
REFERENCES 19
APPENDIX 22


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