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研究生:柯宜禎
研究生(外文):Yi-jhen Ke
論文名稱:發展及評估以無針給藥儀經皮給藥:以diclofenac sodium為模式藥物
論文名稱(外文):The development and evaluation of needle-free injection transdermal drug delivery system:diclofenac sodium as model drug
指導教授:洪伯達
指導教授(外文):Po-da Hong
口試委員:洪伯達
口試委員(外文):Po-da Hong
口試日期:2014-07-03
學位類別:碩士
校院名稱:國立臺灣科技大學
系所名稱:醫學工程研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:76
中文關鍵詞:經皮穿透液體藥物遞送儀器雙氯芬酸鈉腳掌熱痛閾值測試局部鎮痛
外文關鍵詞:Transdermal drug deliveryLiquid medication dispenserDiclofenac sodiumPlantar testLocal analgesic
相關次數:
  • 被引用被引用:0
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本研究目的為評估以新型經皮給藥儀-liquid medication dispenser (LMD)經由皮膚滲透傳遞模式藥物(diclofenac sodium),LMD給藥儀是一台以氣體壓力將液體藥物傳輸進入皮膚達到局部鎮痛效果。
評估LMD給藥儀的性質及特性,包括LMD給藥儀的最佳氣體壓力、滲透深度、經皮滲透累積量以及動物試驗之局部鎮痛效果。利用仿體得知LMD給藥儀的最佳氣體壓力為100 psi;在豬耳皮的滲透深度方面,氣體壓力100 psi注射50發可達136.44±10.90 μm及100發可達165.43±7.19 μm;於diclofenac sodium經皮滲透累積滲透量方面,有使用LMD給藥儀的組別相較於控制組增加了39%,且滲透流量比控制組多33%及遲滯時間減少12%;最後,大鼠腳掌熱痛閾值測試中,以LMD給藥儀給予diclofenac sodium,藥物效能可持續到60分鐘。
由研究結果得知,LMD給藥儀確實能克服皮膚角質層的屏障及增加藥物滲透能力;由動物實驗中證實,LMD給藥儀搭載diclofenac sodium確實能局部給藥達到局部鎮痛效果。LMD給藥儀具有很大的潛力運用在局部經皮給藥系統。
An novel transdermal drug delivery device (liquid medication dispenser, LMD) used to evaluate skin permeability of model drug (diclofenac sodium). It’s a machine combining with gas pressure offer a new effective deliver drug to skin for local analgesic therapy.
The optimal condition of the LMD including the optimal gas pressure and the permeation depth was evaluated. The diffusion model (Franz static diffusion cell) used to evaluate the skin permeation cumulative amount profile. And in vivo local analgesic effects were evaluated in animal model.
Using the agar block model, the optimal gas pressure of LMD is 100 psi, which resulted in the permeation depth of porcine ear skin 136.44±10.90 μm (50 shots) and 165.43±7.19 μm(100 shots). In skin permeation of diclofenac sodium cumulative amount study, the LMD group increased 39%, steady state flux increased 33% and lag time decreased 12% compare to control group (no treatment). In rat’s plantar test, the effect of local analgesic persisted in 60 minutes in LMD group.
Our results showed that LMD can overcome stratum corneum and enhance skin permeability. In animal test, LMD can be used in local therapy. The LMD have great potential to local transdermal drug delivery system.
中文摘要 Ⅰ
英文摘要 Ⅱ
誌 謝 Ⅲ
目 錄 Ⅳ
圖目錄 Ⅶ
表目錄 Ⅷ
第一章 緒論 1
1.1 引言 1
1.2 人體皮膚構造簡介 2
1.3 經皮滲透路徑 5
1.4 藥物經皮滲透理論 7
1.4.1 體外經皮滲透實驗(in vitro) 7
1.4.2 理論及應用 10
1.5 藥物經皮傳輸系統 14
1.6 Diclofenac sodium簡介 20
1.6.1 物化性質 20
1.6.2 藥理作用 21
1.6.3 藥物動力學性質 23
1.6.4 Diclofenac sodium製劑 24
1.7 動物疼痛測量模式 25
1.8 研究動機與目的 26
第二章 研究設計與方法 27
2.1 研究設計 27
2.1.1實驗設計 27
2.1.2實驗架構與流程 27
2.2 藥物、試劑與設備 28
2.2.1 藥物及材料 28

2.2.2 實驗儀器設備 29
2.3 Diclofenac sodium 製劑及分析檢測 30
2.3.1 Diclofenac sodium製劑 30
2.3.2 Diclofenac sodium定量分析 31
2.4 體外實驗. 32
2.4.1 皮膚前處理 32
2.4.2 LMD給藥儀評估 34
2.4.2.1 氣體壓力評估 34
2.4.2.2 滲透深度 35
2.4.2.3 打入皮膚量評估 36
2.4.3 體外經皮滲透實驗 37
2.5 動物實驗 40
2.6 資料分析 43
第三章 實驗結果 45
3.1 Diclofenac sodium製劑及分析檢測 45
3.1.1 Diclofenac sodium 製劑 45
3.1.2 Diclofenac sodium定量分析 48
3.2 體外實驗 50
3.2.1 LMD給藥儀評估 50
3.2.2 體外經皮滲透實驗 57
3.3動物實驗 60
第四章 結果討論 63
4.1 Diclofenac sodium複合溶劑 63
4.2 LMD給藥儀評估 64
4.2.1體外實驗 64
4.3 動物實驗 67
第五章 結論 69
參考文獻 70
附件1. 動物實驗申請表 75
附件2. 動物實驗資格證明 76
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