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研究生:陳力語
研究生(外文):Li-Yu Chen
論文名稱:菱角殼纖維與聚羥基烷酸酯複合材之機械性質、生物相容性與生物降解性之研究
論文名稱(外文):The mechanical properties, biocompatibility and biodegradability of chestnut shell fibre and polyhydroxyalkanoate composites
指導教授:蘇舜恭
指導教授(外文):Shuenn-kung Su
口試委員:蘇舜恭
口試委員(外文):Shuenn-kung Su
口試日期:2014-07-29
學位類別:碩士
校院名稱:國立臺灣科技大學
系所名稱:材料科學與工程系
學門:工程學門
學類:材料工程學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:76
中文關鍵詞:聚羥基烷酸酯菱角殼纖維生物相容性生物降解性
外文關鍵詞:Poly(hydroxyalkanoate)Chestnut shell fibreBiocompatibilityBiodegradation
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本研究為評估菱角殼纖維(CSF)與聚羥基烷酸酯(PHA)複合材(PHA/CSF)以及CSF與PHA接枝甲基丙烯酸縮水甘油脂(PHA-g-GMA)複合材(PHA-g-GMA/CSF)之機械性質、生物相容性與生物降解性。
研究發現PHA-g-GMA/CSF相較於PHA/CSF有較好的機械性質,CSF在縮合反應時可以均勻的分散在PHA-g-GMA的基質中;將人包皮成纖維細胞(HFF)附著於這兩種複合材上以測生物相容性;附著於PHA/CSF複合材上FBs之細胞增生率與產生的膠原蛋白皆較PHA-g-GMA/CSF複合材為佳;PHA-g-GMA/CSF複合材比PHA/CSF有較好的抗水性;將兩種複合材放入放射性根瘤菌(Rhizobium radiobacter)中皆有重量的損失,及指出兩種複合材皆有生物降解性,且生物降解性會隨CSF濃度上升而升高,另PHA/CSF與PHA-g-GMA/CSF複合材之生物降解性皆比純的PHA好。
The mechanical properties, biocompatibility and biodegradability of composite materials made from chestnut shell fibre (CSF) and poly(hydroxyalkanoate) (PHA), as well as CSF and glycidyl methacrylate grafted PHA (PHA-g-GMA), were evaluated. Composites formed from PHA-g-GMA/CSF were found to have noticeably superior mechanical properties compared with those of PHA/CSF. CSF could be homogeneously dispersed in the PHA-g-GMA matrix as a result of condensation reactions. Human foreskin fibroblasts (FBs) were seeded on these two series of composites to characterise the biocompatibility properties. FB proliferation and collagen production on the PHA/CSF series of composites were superior to that on the PHA-g-GMA/CSF composites. PHA-g-GMA/CSF was found to be more water resistant than PHA/CSF, although the weight loss of both composites buried in Rhizobium radiobacter compost indicated that both were biodegradable, especially at high levels of CSF substitution. Furthermore, the PHA/CSF and PHA-g-GMA/CSF composites were more biodegradable than pure PHA.
摘要 I
ABSTRACT II
誌謝 III
目錄 IV
圖索引 VIII
表索引 IX
第一章 緒論 1
1.1 研究背景與目的 1
第二章 文獻回顧 4
2.1 PHA之介紹 4
2.2 合成PHA之主要微生物 8
2.3 PHAs的發酵培養方式 10
2.4 活性污泥合成PHAs之方法介紹 11
2.4.1 活性污泥合成PHAs原理 11
2.4.2 EBPR工藝用於PHAs生產的特點和流程 12
2.5 活性污泥合成PHA的影響因素 14
2.5.1 底物的影響 14
2.5.2 營養元素比例的影響 15
2.5.3 SRT的影響 16
2.5.4 pH值的影響 16
2.5.5 溫度的影響 17
2.6 聚合物改性介紹 18
2.7 PHAs之改性介紹 19
2.7.1 生物改性 19
2.7.1.1 PHBV之生物改性 20
2.7.1.2 P3/4HB之生物改性 20
2.7.1.3 PHBHHx之生物改性 21
2.7.2 化學改性 21
2.7.3 物理改性 22
2.8 PHBV接枝GMA (PHBV-g-GMA)之介紹 23
2.8.1 GMA接枝於PHBV上之反應機制 23
2.8.2 PHBV-g-GMA之熱性能與結晶結構 25
2.9 GMA之介紹 26
2.10 引入菱角殼(CSF)之目的 28
2.10.1 農業廢棄物 28
第三章 實驗方法與原理 30
3.1 實驗藥品 30
3.2 實驗儀器 31
3.3 PHA-g-GMA共聚物的製備 32
3.4 Chestnut shell fibre (CSF)的製備 33
3.5 PHA/CSF與PHA-g-GMA/CSF複合材的製備 34
3.6 物理性質測定方法 35
3.6.1 CPMAS-NMR(交叉極化魔角旋轉NMR)測定 35
3.6.2 示差掃描熱卡計量測(DSC) 35
3.6.3 黏度計 36
3.7 機械測試 36
3.8 複合材表面型態觀察 36
3.9 Human foreskin fibroblasts 在PHA系複合材上之生物功能性 37
3.9.1 細胞與培養基 37
3.9.2 以MTT試驗測試細胞存活率分析 37
3.9.3 膠原蛋白定量 38
3.10 吸水率 39
3.11 微生物測試樣品的配製並置入R. radiobacter (放射型根瘤菌)中 40
第四章 結果與討論 41
4.1 PHA與其複合材之物理性質 41
4.2 PHA與其複合材之表面型態與機械性質 43
4.3 PHA與其複合材之熱學特性 47
4.4 PHA與其複合材之生物相容性 48
4.5 PHA與其複合材之吸水率 51
4.6 PHA與其複合材之生物降解性 52
第五章 結論 56
參考文獻 57
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