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研究生:陳光瑩
研究生(外文):Tran Quang Dan
論文名稱:利用菸草表達豬第二型環狀病毒融合蛋白之研究
論文名稱(外文):Studies on the Expression of Recombinant ORF3 Fusion Proteins of Porcine Circovirus Type 2 in Transgenic Tobacco
指導教授:黃鵬林
指導教授(外文):Huang, Pung-Ling
口試委員:杜宜殷黃贊勳
口試委員(外文):Do, Yi-YinHwang, Tzann-Shun
口試日期:2014-06-30
學位類別:碩士
校院名稱:中國文化大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:英文
論文頁數:93
中文關鍵詞:quangdan
外文關鍵詞:quangdan
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Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome in pigs and has impact on swine-producing industry worldwide. The open reading frame 3 (ORF3) of PCV2 which encodes an 11.9 kDa ORF3 protein has been found to be involved in the pathogenesis of PCV2 infection by its apoptotic activity. The ORF3–based antigens thus represent a traditional approach to develop efficacious oral vaccines for immunization against PCV2. In this study, various ORF3-containing expression vectors, namely pGKF/U-ORF3, pGKF/U-L-ORF3, pGKF/U-ORF2-3b, and pGKF/U-L-ORF2-3b, were transformed to tobacco plants in order to produce the respective subunit recombinant vaccines. Following the Agrobacterium tumefaciens-mediated transformation of 200 leaf samples, 20 ORF3, 15 L-ORF3, 14 ORF2-3b, and 14 L-ORF2-3b putative transgenic tobacco plants were regenerated on the selective medium supplied with 200 mg/L kanamycin and showed GUS expression. The expected sequences of respective ORF3-containing trangenes, 359 bp, 369 bp, 1056 bp, and 1439 bp were amplified from genomic DNA of 19 ORF3 (9.5%), 14 L-ORF3 (7.0%), 12 ORF2-3b (6.0%), and 12 L-ORF2-3b (6.0%) GUS-positive transgenic plants, using specific pair of primers in PCR analysis. On the other hand, integration of 1-2 copies of independent ORF3-containing transgenes into tobacco genome was determined by means of Southern hybridization. The mRNA transcripts of particular transgenes were also found in the PCR-positive transgenic plants. Particularly, expression of respective ORF3 fusion proteins was qualitatively examined via immunobloting (Western blot) assay. However, the results have not clearly shown presence of target proteins, due to either low level of expression or interfering background.
Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome in pigs and has impact on swine-producing industry worldwide. The open reading frame 3 (ORF3) of PCV2 which encodes an 11.9 kDa ORF3 protein has been found to be involved in the pathogenesis of PCV2 infection by its apoptotic activity. The ORF3–based antigens thus represent a traditional approach to develop efficacious oral vaccines for immunization against PCV2. In this study, various ORF3-containing expression vectors, namely pGKF/U-ORF3, pGKF/U-L-ORF3, pGKF/U-ORF2-3b, and pGKF/U-L-ORF2-3b, were transformed to tobacco plants in order to produce the respective subunit recombinant vaccines. Following the Agrobacterium tumefaciens-mediated transformation of 200 leaf samples, 20 ORF3, 15 L-ORF3, 14 ORF2-3b, and 14 L-ORF2-3b putative transgenic tobacco plants were regenerated on the selective medium supplied with 200 mg/L kanamycin and showed GUS expression. The expected sequences of respective ORF3-containing trangenes, 359 bp, 369 bp, 1056 bp, and 1439 bp were amplified from genomic DNA of 19 ORF3 (9.5%), 14 L-ORF3 (7.0%), 12 ORF2-3b (6.0%), and 12 L-ORF2-3b (6.0%) GUS-positive transgenic plants, using specific pair of primers in PCR analysis. On the other hand, integration of 1-2 copies of independent ORF3-containing transgenes into tobacco genome was determined by means of Southern hybridization. The mRNA transcripts of particular transgenes were also found in the PCR-positive transgenic plants. Particularly, expression of respective ORF3 fusion proteins was qualitatively examined via immunobloting (Western blot) assay. However, the results have not clearly shown presence of target proteins, due to either low level of expression or interfering background.
ABSTRACT ii
ACKNOWLEDGMENT iii
CONTENTS iv
LIST OF TABLES vi
LIST OF FIGURES vii
ABBREVIATIONS ix
CHAPTER ONE INTRODUCTION 1
1.1 Research Background and Motivation 1
1.2 Purpose of Research 3
CHAPTER TWO REVIEW OF LITERATURE 4
2.1 A Review of Porcine Circovirus Type 2 Virus 4
2.1.1 Characterization of Porcine Circovirus Type 2 and Associated Diseases in PCV2-Infected Swines 4
2.1.2 Open Reading Frame 2 of PCV2 7
2.1.3 Open Reading Frame 3 Protein of PCV2 8
2.2 Escherichia coli Heat-Labile Enterotoxin B Subunit and Its Characterization 9
2.3 Review of Transgenic Plants for Expression of Vaccine Antigens 11
2.3.1 The Advantages of Using Plants as Host Systems for Expression of Vaccine Antigens 11
2.3.2 General Molecular Components Affecting Protein Expression in Plants 13
2.3.3 Transgenic Plants for the Production of Veterinary Vaccines 15
2.4 Tobacco, A Highly Efficient Green Bioreactor for Production of Recombinant Vacinnes. 21
2.5 Role of Fusion Factors Enhancing Efficient Accumulation of Recombinant Proteins in Plants 23
2.5.1 Endoplasmic Reticumlum Retention Signal 23
2.5.2. Affinity His-tag 24
CHAPTER THREE MATERIALS AND METHODS 26
3.1 Experimental Materials 26
3.1.1 Expression Vectors 26
3.1.2 Plant Materials 27
3.1.3 Bacterial Strains 27
3.1.4 Laboratory Facilities and Equipments 28
3.2 Experimental Methods 28
3.2.1 Electroporation 28
3.2.2 Plant Transformation and Regeneration 28
3.2.3 GUS Staining 29
3.2.4 Polymerase Chain Reaction (PCR) 29
3.2.5 Southern Blot Analysis 31
3.2.6 Reverse Transcription-PCR (RT-PCR) 32
3.2.7 Immunoassay 32
CHAPTER FOUR RESULTS 35
4.1 Expression ORF3 Protein Antigen in Escherichia coli BL21 (DE3) 35
4.1.1 Construction of Expression Vector 35
4.1.2 Expression and Purification of Recombinant ORF3 Protein 35
4.2 Transformation and Regeneration of Transgenic Tobacco Plants 36
4.3 Gus Expression 36
4.4 Characterization of Transgenic Tobacco 37
4.3.1 Polymearase Chain Reaction 37
4.3.2 Southern Blot Analysis of Transgenic Plants 37
4.3.3 Transcription of Transgenes 38
4.5 Expression of ORF3 Fusion Proteins in Transgenic Tobacco 39
TABLES and FIGURES 40
CHAPTER FIVE DISCUSSION 61
CHAPTER SIX CONCLUSION AND FUTURE DIRECTION 65
REFERENCES 66

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