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研究生:劉力綾
研究生(外文):Liu,Li-Ling
論文名稱:萊姆薄荷及綠薄荷精油之抗菌、抗氧化及抑制小鼠黑色素腫瘤細胞B16F10生成黑色素之性質探討
論文名稱(外文):Antioxidant, antibacterial, and anti-melanogenic properties of lime mint and spearmint essential oils
指導教授:楊昭順
指導教授(外文):Yang, Chao-Hsun
口試委員:張聰民黃煜珺
口試委員(外文):Chang,Tsong-MinHuang,Yu-Chun
口試日期:2014-07-24
學位類別:碩士
校院名稱:靜宜大學
系所名稱:化粧品科學系
學門:民生學門
學類:美容學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:77
中文關鍵詞:萊姆薄荷綠薄荷石竹烯黑色素抗氧化抗菌
外文關鍵詞:Mentha aquatic × Mentha suareolensMentha spicataβ-caryophyllenemelaninantioxidantantibecterial
相關次數:
  • 被引用被引用:5
  • 點閱點閱:989
  • 評分評分:
  • 下載下載:125
  • 收藏至我的研究室書目清單書目收藏:1
芳香植物精油已多方運用在食品、化妝品工業、醫藥等用途上。薄荷是一種傳統又廣效的藥草。精油是由植物提煉而來,主要成分為為脂溶性小分子。本研究主要探討萊姆薄荷精油和綠薄荷精油之抗氧化能力、抗菌能力,並以B16F10小鼠黑色素細胞瘤評估其對黑色素生成之影響。結果顯示,萊姆薄荷精油和綠薄荷精油均可抑制B16F10細胞內酪胺酸酶活性及黑色素生成。而精油中之β-caryophyllene成分可藉由負調控MITF調節因子,進而降低酪胺酸酶及酪胺酸酶相關蛋白量之生合成,進而抑制黑色素之生成。在抗氧化試驗中,萊姆薄荷精油和綠薄荷精油均有清除DPPH自由基能力和還原力。在抗菌試驗中,萊姆薄荷精油和綠薄荷精油亦均有抑制金黃色葡萄球菌及大腸桿菌之能力。因此,薄荷精油可添加於化妝品配方中,作為美白或抗氧化之活性成分。
The fresh and dried leaves of mint are usually used as flavorings or spices in a wide variety of food and cosmetics, and pharmaceutical industry for a long history. They contain biologically active ingredients and also used as complementary and alternative medicine. The purpose of this study is to investigate the antioxidant, antibacterial, and anti-melanogenic properties of lime mint (Mentha aquatic × Mentha suareolens) and spearmint (Mentha spicata) essential oils. Our results indicated that both lime mint and spearmint essential oil could decrease the intracellular tyrosinase activities and then inhibited the melanogenesis in B16F10 cells. The β-caryophyllene, one of the main compound in both mint essential oil, could reduce melanin production via down-regulation of microphthalmia-associated transcription factor to decrease the expression of tyrosinase and tyrosinase related protein 1/2 in B16F10 cells. The antioxidative activities assessment showed that both lime mint and spearmint essential oils had DPPH scavenging radical activity and reducing power. Both of the essential oils had the antibacterial abilities against Staphylococcus aureus and Escherichia coli .Hence, both mint essential oils could be applied into cosmetic formulation and act as a whitening or antioxidative active ingredient.
中文摘要 Ι
英文摘要 II
目錄 III
圖目錄 VI
名詞縮寫 VIII
第一章、緒論 1
第一節、黑色素 1
一、黑色素細胞簡介 1
二、黑色素生合成路徑 6
三、黑色素機轉訊息傳遞路徑 8
第二節、精油介紹 11
一、精油 11
二、精油生理活性 13
三、精油之應用 15
第三節、薄荷簡介 16
一、薄荷 16
二、本實驗中的薄荷簡介 17
三、薄荷精油成分 20
四、本實驗中精油的主要化合物簡介 22
第四節、研究動機與實驗架構 23
一、研究動機 23
二、實驗架構 24
第二章、薄荷精油與化合物對黑色素細胞影響 25
第一節、前言 25
第二節、實驗材料 26
一、藥品 26
二、緩衝溶液配製 27
第三節、實驗方法 30
一、細胞來源 30
二、細胞存活率測定 30
三、黑色素含量測定 31
四、Cell-free酪胺酸酶活性 32
五、酪胺酸酶活性染色 32
六、西方墨點法 33
七、統計分析 34
第四節、結果 35
一、細胞存活率測定 35
二、黑色素含量測定 36
三、Cell-free酪胺酸酶活性 37
四、酪胺酸酶活性染色 37
五、西方墨點法 38
第五節、討論 40
第三章、薄荷精油之抗氧化及抗菌能力探討 41
第一節、前言 41
第二節、實驗材料 42
一、藥品 42
二、菌株 42
三、培養基配製 43
四、抗生素 44
第三節、實驗方法 44
一、DPPH自由基清除能力 44
二、還原力測定 45
三、ABTS自由基清除能力 47
四、菌株培養 48
五、抑菌圈抑制菌活性之評估 48
第四節、結果 49
一、DPPH自由基清除能力 49
二、還原力測定 49
三、ABTS自由基清除能力 49
四、薄荷精油對金黃色葡萄球菌抑菌效果 50
五、薄荷精油對大腸桿菌抑菌效果 50
六、薄荷精油對白色念珠菌抑菌效果 50
第四章、結論 51
參考文獻 52

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