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研究生:李昀臻
研究生(外文):Li, Yunjhen
論文名稱:探討口服鮭魚對Sprague-Dawley 大鼠創傷修復增生期之影響
論文名稱(外文):Effect of Oral Salmon on Wound Healing of Proliferative Phase in Sprague-Dawley Rats
指導教授:黃延君
指導教授(外文):Huang, Yanjiun
口試委員:張珍田王如玉
口試委員(外文):Jhang, JhentianWang, Ruyu
口試日期:2013-09-03
學位類別:碩士
校院名稱:靜宜大學
系所名稱:食品營養學系
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2013
畢業學年度:102
語文別:中文
論文頁數:78
中文關鍵詞:創傷修復鮭魚SD 大鼠傷口收縮百分比氫脯胺酸
外文關鍵詞:wound healingsalmonSprague-Dawley ratswound contractionhydroxyproline
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鮭魚為台灣常見食用魚類之一,富含與創傷修復相關之營養成分,屬於營養價值較高之食材,但缺乏對於創傷修復相關研究。創傷修復主要分為四個時期:凝血期、發炎期、增生期與重塑期,增生期膠原蛋白形成有利於細胞外基質和肉芽組織合成填補損傷之部位,為修復之重要關鍵。因此,本實驗探討口服鮭魚對傷口增生期修復之影響。鮭魚以冷凍乾燥製成魚粉後,進行成分分析,粗蛋白含量為48.19 %、粗脂肪為47.72 %、無氮萃取物為4.10 %,而氫脯胺酸735 mg/100 g。動物實驗分為兩個部分: 第一部份為劑量試驗,SD雄性大鼠體重約300-400 克時,每隻大鼠背上製造3 個傷口,分為三組劑量(0.94、2.36、4.72 g/kg BW/day),連續口服6 天,於第7天犧牲。結果顯示體重、飲水量及攝食量三組無顯著差異,口服2.36 g/kg BW/day 鮭魚,其傷口收縮百分比顯著高於其他兩組。第二部分為口服試驗,製造傷口模式與劑量試驗相同,分為對照組與鮭魚組,連續口服10 天2.36 g/kg BW/day 鮭魚,於第4、7、10天犧牲,取傷口組織測定血小板源性生長因子(platelet-derivedgrowth factor, PDGF)、轉化生長因子 (transforming growth factor,TGF-β)、醣醛酸、六碳醣胺、氫脯胺酸及傷口收縮百分比。結果顯示兩組體重、飲水量及攝食量無顯著差異,第4 天兩組創傷修復相關指標也無顯著差異。第7 天鮭魚組傷口PDGF、TGF-β、醣醛酸、氫脯胺酸含量顯著增加,傷口收縮百分比也顯著上升。而第10 天鮭魚組傷口醣醛酸與六碳醣胺顯著增加,其於皆無顯著差異。綜合以上可得知給予創傷大鼠口服2.36 g /kg BW/day 鮭魚,在創傷後第7 天最能顯著增加傷口PDGF、TGF-β、醣醛酸、氫脯胺酸含量。增生期傷口修復需要大量的膠原蛋白,建造修補傷口,鮭魚及鮭魚中的氫脯胺酸提供傷口修復合適的材料,達到促進傷口收縮,進而幫助傷口癒合。
Salmon (Salmo salar Linnaeus) is one of the commonly edible fish in Taiwan, which is rich of nutrients for its beneficial effect for wound healing, belonging to a higher nutritional value of the food, but the lack of relevant studies for wound healing. Wound healing includes phases of coagulate, inflammatory, proliferative andremodeling, this is critical phase of proliferative, because the repair process to increase production of collagen formation, and the formation of granulation tissue and extracellular matrix (ECM) at the site of injury. The purpose of this study is to explore the effect of oral salmon on wound healing of phase proliferative in Sprague-Dawley rats.Salmon was boiled and freeze-dried for used. The proximentanalysis was assayed before experiment, crude protein 48.19 %, crude
fat 47.72 %, NFE 4.10 %, hydroxyproline 735 mg/100g. In the experiment I (dose experiment), male SD rats were body weight of 300-400 g, each rats was created 3 wound on the back, rats are divided into three dose (0.94, 2.36, 4.72 g/kg BW/day) of salmon was given orally for six days and sacrificed on the 7th day. These wounds were used to determine the wound contraction. Results showed oral of 2.36 g/kg BW/day salmon, its wound contraction was significantly higher than the other two groups. In the experiment ΙΙ (oral experiment),wound model is as same as dose experiment, and then doses of results selection 2.36 g/kg BW/day, rats are divided into control and salmon group, given orally for ten days and sacrificed on the 4th, 7th, 10th day.These wounds were used to determine the PDGF, TGF-β, uronic acid,hexosamin, hydroxyproline, wound contraction. Results showed salmon group wound tissue were no significant effect on the 4th day.Wound tissue PDGF, TGF-β, uronic acid, hydroxyproline content increased, wound contraction also significantly rise on the 7th day. In addition to uronic acid and hexosamine, salmon group the rest was no significant effects on the 10th day.In summary, salmon was given orally 2.36 g/kg BW/day can improve significantly PDGF, TGF-β, uronic acid, hydroxyproline content on the 7th day . Proliferative phase of wound healing requires a large amount of collagen, construction repair wounds, salmon and salmon of hydroxyproline provide appropriate materials in wound repair, promote wound contraction, thereby helping wound healing.
目錄 Ι
表目錄 ΙV
圖目錄 V
中文摘要 VΙ
英文摘要 VΙΙΙ
第一章 緒言 1
第二章 文獻回顧 3
第一節 創傷修復 3
一、創傷的定義及分類 3
二、創傷引起之生理變化 4
三、創傷修復相關營養代謝 5
四、創傷修復機轉 7
五、大鼠創傷修復模式 10
第二節 創傷修復相關指標 11
一、生長因子 11
二、生化指標 12
第三節 實驗材料介紹 15
一、台灣對鮭魚供需概況 15
二、鮭魚介紹與營養成分 15
三、鮭魚生理功能研究 16
第三章 材料與方法 18
第一節 實驗材料 18
第二節 實驗儀器 20
第三節 實驗藥品 21
第四節 實驗動物 23
第五節 實驗動物飼料組成分 23
第六節 實驗設計 24
一、劑量試驗 24
二、口服試驗 27
三、檢測方法 29
四、統計方法 36
第四章 結果 37
第一節 鮭魚組成分分析 37
第二節 體重變化 37
第三節 攝食量與飲水量 38
第四節 血小板源性生長因子 39
第五節 轉化生長因子 39
第六節 醣醛酸 39
第七節 六碳糖胺 40
第八節 氫脯胺酸 40
第九節 傷口收縮 41
第五章 討論 42
第六章 結論 50
第七章 參考文獻 71
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