跳到主要內容

臺灣博碩士論文加值系統

(44.192.79.149) 您好!臺灣時間:2023/06/03 00:39
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:陳凱祥
研究生(外文):Chen Kai-Hsiang
論文名稱:固態培養紅樟芝與白樟芝菌絲體萃取物之抗氧化能力與抗前列腺癌細胞之活性探討
論文名稱(外文):The antioxidant activity and antiproliferativeeffect of mycelia extracts by solid-statefermentation of red and white Antrodiacinnamomea on DU-145 prostate cancer cells
指導教授:張春生
學位類別:碩士
校院名稱:南台科技大學
系所名稱:生物科技系
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:103
畢業學年度:102
語文別:中文
論文頁數:81
中文關鍵詞:固態培養抗前列腺癌牛樟芝抗氧化
外文關鍵詞:Antrodia cinnamomeaantioxidantanti prostate cancer
相關次數:
  • 被引用被引用:1
  • 點閱點閱:1151
  • 評分評分:
  • 下載下載:57
  • 收藏至我的研究室書目清單書目收藏:0
牛樟芝(Antrodia cinnamomea)是台灣特有藥用菇菌,僅生長於台灣特有之牛樟
樹(Cinnamomum kanehirai) 的腐朽心材内壁,或枯死倒伏之牛樟樹幹的潮濕表面,
近來出現變種白色牛樟芝,同為臺灣著名且珍貴之藥用真菌,近來科學研究發現
其可能具有醫療功效。
目前對於白樟芝的研究甚少,因此本研究從事固態培養紅樟芝與白樟芝,比
較酚類及粗三萜生物活性成分、抗氧化能力與對前列腺癌細胞之抗轉移詴驗、與
抗增生詴驗,並以流式細胞儀PI 染色法觀察細胞週期分佈,探討抗前列腺癌之作
用機制。
結果顯示紅樟芝與白樟芝熱水萃取物酚類含量最高,乙醇萃取物粗三萜含量
最高。紅樟芝抗氧化能力優於白樟芝。紅樟芝與白樟芝熱水萃取物皆能夠給予
CHO-K1 細胞增加抗氧化能力,兩者保護能力明顯優於修復能力。樟芝抗前列腺癌
細胞DU-145 活性,紅樟芝與白樟芝乙酸乙酯萃取物抑制增生能力最佳,白樟芝抑
制轉移能力優於紅樟芝。以紅樟芝與白樟芝乙酸乙酯萃取物處理前列腺癌細胞
DU-145,可使DU-145 細胞週期停滯在G2/M 期,抑制癌細胞增生。
Antrodia cinnamomea is well known in Taiwan as an expensive medicinal fugi.
Recently,. Both red and white Antrodia cinnamomea (RAC and WAC) are folk
medicinal mushroom commonly used in Taiwan, the latter recognized as a variant of
Antrodia cinnamomea.
In this study, we respectively used solid substrates to culture RAC and WAC strain
by solid-state fermentation, compared different extracts to evaluated phenolic content,
crude triterpenoids content, antioxidant capacity and antiproliferatiive effect on DU-145
prostate cancer cells.
The results showed that the phenolic content of hot water (HW) extract and the
crude triterpenoids content 95% ethanol extract (EtOH) own the highest conetnt. HW
extract can provide CHO-K1 cells to resist the oxidative stress from H2O2. The
protective antioxidant capacity of RAC HW extract is better than that of WAC. RAC
and WAC ethyl acetate (EA) own the best antiproliferation in DU-145 cells, and WAC
inhibited DU-145 migration ability was significantly better than RAC. RAC and WAC
EA extracts treatment DU-145 cells through G2/M phase cell cycle arrest and induction
of apotosis.in. Our result showed that RAC and WAC own antioxidant capacity, and anti
prostate cancer, suggesting that it may be a potential preventive or therapeutic prostate
cancer.
摘要 ...................................................................................................................................I
ABSTRACT..................................................................................................................... II
目次 ................................................................................................................................ III
表目錄 ............................................................................................................................VI
圖目錄 ...........................................................................................................................VII
第一章、緒論 .................................................................................................................. 1
1.1 前言...................................................................................................................... 1
第二章、文獻回顧 .......................................................................................................... 2
2.1 抗氧化概述............................................................................................................ 2
2.1.1 自由基對生物體的影響................................................................................ 2
2.1.2 抗氧化劑........................................................................................................ 4
2.2 前列腺癌................................................................................................................ 8
2.2.1 前列腺和前列腺癌的介紹............................................................................. 8
2.2.2 前列腺癌的轉移與侵略................................................................................. 8
2.2.3 前列腺癌的分類與分期................................................................................. 9
2.2.4 前列腺癌的治療........................................................................................... 10
2.3 牛樟芝簡介.......................................................................................................... 11
2.3.1 牛樟芝命名及分類....................................................................................... 11
2.3.2 牛樟芝的形態............................................................................................... 13
2.3.3 牛樟芝的活性成份....................................................................................... 14
2.3.4 牛樟芝的藥理活性....................................................................................... 16
2.4 固態與液態發酵.................................................................................................. 20
2.5 研究動機.............................................................................................................. 23
2.6 研究架構.............................................................................................................. 24
第三章、材料與方法 .................................................................................................... 25
3.1 實驗材料.............................................................................................................. 25
3.1.1 設備............................................................................................................... 25
3.1.2 藥品............................................................................................................... 26
3.2 牛樟芝固態發酵產物製備及細胞培養 .............................................................. 28
3.3 分析方法 .............................................................................................................. 31
3.3.1 Folin-Ciocalteu’s phenol reagent ................................................................... 31
3.3.2 Vanillin-Acetic acid 香草醛-冰醋酸法........................................................ 32
3.3.3 ABTS 自由基清除能力............................................................................... 33
3.3.4 OH 自由基清除能力..................................................................................... 35
3.3.5 還原力........................................................................................................... 36
3.3.6 比較不同萃取物對固態發酵酚含量及抗氧化能力................................... 37
3.3.7 紅樟芝與白樟芝熱水萃取物對CHO-K1 細胞抗氧化能力....................... 37
3.3.8 比較不同萃取物對固態發酵粗三萜含量及細胞毒性測詴-MTT assay.... 38
3.3.9 細胞形態觀察(cell morphology) ................................................................ 39
3.3.10 轉移詴驗(migration assay)........................................................................ 39
3.3.11 細胞週期測定(Cell Cycle Analysis)-Propidium (PI) stain ......................... 40
4.1 固態發酵不同萃取物對酚類含量、粗三萜含量及抗氧化能力之比較.......... 41
4.2 紅樟芝與白樟芝熱水萃取物對 CHO-K1 細胞抗氧化能力.............................. 44
4.3 比較固態發酵不同萃取物對細胞毒性測詴-MTT ASSAY................................. 48
4.4 紅樟芝與白樟芝乙酸乙酯萃取物處理 DU-145 細胞之形態變化................... 55
4.5 比較紅樟芝與白樟芝乙酸乙酯萃取物抑制DU-145 細胞之轉移能力........... 57
4.6 紅樟芝與白樟芝乙酸乙酯萃取物對 DU-145 細胞之細胞週期的影響........... 60
第五章、結論................................................................................................................ 65
第六章、參考文獻 ........................................................................................................ 66
Biel, W., Bobko, K., Maciorowski, R. Chemical composition and nutritive value of
husked and naked oats grain. Journal of Cereal Science, 49, 413-418, 2009.
Cai, S., Wang, O., Wu, W., Zhu, S., Zhou, F., Ji, B., Gao, F., Zhang, D., Liu, J., Cheng,
Q. Comparative study of the effects of solid-state fermentation with three
filamentous fungi on the total phenolics content (TPC), flavonoids, and antioxidant
activities of subfractions from oats (Avena sativa L.). Agricultural and Food
Chemistry, 60, 507-513, 2012.
Chang, C. Y., Huang, Z. N., Yu, H. H. The adjuvant effects of Antrodia camphorata
extracts combined with antitumor agents on multidrug resistant human hepatoma
cells. Journal of Ethnopharmacology, 118, 387-395, 2008.
Chang, T. T., Chiu, W. H ., Hua, J. The cultural atlas of wood-inhabiting Aphyllophorales
in Taiwan. Mycology. Monograph, 11, 1-118, 1997.
Chang, T. T., Chou, W. N. Antrodia cinnamomea sp. nov. on Cinnamomum kane hirai in
Taiwan. Mycological Research, 99, 756-758, 1995.
Chang, T. T., Chou, W. N. Antrodia cinnamomea reconsidered and A. salmonea sp. nov.
on Cunninghamia konishii in Taiwan. Botanical Bulletin- Academia Sinica, 45, 347-352,
2004.
Chang, Y. C., Chang, T. J., Jiang, Y. D., Kuo, S. S., Lee, K. C., Chiu, K. C., Chuang. L.
M. Association study of the genetic polymorphisms of the transcription factor
7-like 2 (TCF7L2) gene and type 2 diabetes in the Chinese population. Diabetes,
56, 2631-2637, 2007
Chen, C. C., Chyau, C. C., Hseu, T. H. Production of a COX-2 inhibitor,
2,4,5-trimethoxybenzaldehyde, with submerged cultured Antrodia camphorata.
Letters in Applied Microbiology, 44, 387-392, 2007.
Chen, C. C., Liu, Y. W., Ker, Y. B. Chemical characterization and anti-inflammatory
effect of polysaccharides fractionated from submerge-cultured Antrodia
camphorate mycelia. Journal of Agricultural and Food Chemistry, 55, 5007-5012,
2007.
Chen, C. C., Shiao, Y. J., Lin, R. D. Neuroprotective diterpenes from the fruiting body
of Antrodia camphorata. Journal of Natural Products, 69, 689-691, 2006.
Chen, C. H., Yang, S.W., Shen, Y. C.New steroid acids from Antrodia cinnamomea, a
fungal parasite of Cinnamomum micranthum. Journal of Natural Products, 58,
1655-1661, 1995.
Chen, C. J., Su, C. H., Lan, M. H. Study on solid cultivation and bioactivity of Antrodia
camphorata. Fungal Sciences, 65-72, 2001.
Chen, J. C. King of Ganoderma: Antrodia camphorata in Taiwan, Yuen Chi Jai Book
Publishing, Taipei, Taiwan, 2nd edition, 2008.
Chen, J. J., Lin, W. J., Liao, C. H., Shieh, P. C. Anti-inflammatory benzenoids from
Antrodia camphorata. Journal of Natural Products, 989-992, 2007.
Chen, K. C., Peng, C. C., Peng, R. Y. Unique formosan mushroom Antrodia camphorata
differentially inhibits androgen-responsive LNCaP and -independent PC-3 prostate
cancer cells. Nutrition and Cancer, 57, 111-121, 2007.
Chen, S. C., Lu, M. K., Cheng, J. J., Wang, D. L. Antiangiogenic activities of
polysaccharides isolated from medicinal fungi. FEMS Microbiology Letters,
247-254, 2005.
Cherng, I. H., Chiang, H. C. Three new triterpenoids from Antrodia cinnamomea. J.
Natural Products. 58, 365-371, 1995.
Cherng, I. H., Wu, D. P., Chiang, H. C. Triterpenoids from Antrodia cinnamomea.
Phytochemistry, 41, 263-267, 1996.
Chiang, C. H., Wu, D. P., Cherng, I. W., Ueng, U. C. A sesquiterpene lactone, phenyl
and biphenyl compounds from Antrodia cinnamomea. Phytochemistry, 39,
613-616, 1995.
Chien, S. C., Chen, M. L., Kuo, H. T., Tsai Y. C., Lin, B. F., Kuo, Y. H.
Anti-inflammatory activities of new succinic and maleic derivatives from the
fruiting body of Antrodia camphorata. Journal of Agricultural and Food Chemistry,
7017-7022, 2008.
Dai, Y. Y., Chuang, C. H., Tsai, C.C., Sio, H. M., Huang, S. C., Chen, J. C., Hu, M. L.
The protection of Antrodia camphorata against acute hepatotoxicity of alcohol in
rats. Journal of Food and Drug Analysis, 11, 177-185, 2003.
Delgado, M. C. O., Tironi, V. A., Añón, M. C. Antioxidant activity of amaranth protein
or their hydrolysates under simulated gastrointestinal digestion. LWT-Food Science
and Technology, 44, 1752- 1760, 2011
Erel, O., A novel automated direct measurement method for total antioxidant capacity
using a new generation, more stable ABTS radical cation. Clinical Biochemistry,
37, 277- 285, 2004.
Folkman, J. Tumor angiogenesis: therapeutic implications. The New England Journal of
Medicine, 1182-1186, 1971.
Folkman, J. What is the role of endothelial cells inangiogenesis?
Laboratory Investigation, 51, 601-604, 1984.
Folkman, J. Angiogenesis in cancer, vascular, rheumatoid and other disease. Nature
Medicine, 1, 27-31, 1995.
Haddad, P. S., Azar, G. A., Groom, S., Boivin, M. Natural health products, modulation
of immune function and prevention of chronic diseases. Evidence-Based
Complementary and Alternative Medicine, 2, 513-520, 2005.
Hsiao, G., Shen, M. Y., Lin, K. H. Antioxidative and hepatoprotective effects of
Antrodia camphorata extract. Journal of Agricultural and Food Chemistry, 51,
3302-3308, 2003.
Huang, K. F., Huang, W. M., Chiang, H. C. Phenyl compounds from Antrodia
cinnamomea. Chinese Pharmaceutical Journal, 53, 327-331, 2001.
Huang, R. L., Huang, Q., Chen, C. F., Chang T. T., Chou C. J. Anti-viral effects of
active compounds from Antrodia camphorata on wild-type and
lamivudine-resistant mutant HBV. Chinese Pharmaceutical Journal, 55, 371-379,
2003.
Hu, H., Zhang, Z., Lei, Z., Yang, Y., Sugiura, N. Comparative study of antioxidant
activity and antiproliferative effect of hot water and ethanol extracts from the
mushroom Inonotus obliquus. Journal of Bioscience and Bioengineering, 107,
42-48, 2009
Hseu, Y. C., Chen, S. C., Chen, H. C., Liao, J. W., Yang, H. L. Antrodia camphorata
inhibits proliferation of human breast cancer cells in vitro and in vivo. Food and
Chemical Toxicology, 46, 2680-2688, 2008a.
Hseu, Y. C., Chen, S. C., Tsai, P. C . Inhibition of cyclooxygenase-2 and induction of
apoptosis in estrogen-nonresponsive breast cancer cells by Antrodia camphorata.
Food and Chemical Toxicology, 45, 1107-1115, 2007.
Hseu, Y. C., Chen, S. C., Yech, Y. J., Wang, L., Yang, H. L. Antioxidant activity of
Antrodia camphorata on free radical-induced endothelial cell damage. Journal of
Ethnopharmacology, 118, 237-245, 2008b.
Hsu, Y. L., Kuo, P. L., Cho, C.Y. Antrodia cinnamomea fruiting bodies extract
suppresses the invasive potential of human liver cancer cell line PLC/PRF/5
through inhibition of nuclear factor κB pathway. Food and Chemical Toxicology,
45, 1249-1257, 2007.
Hsu, Y. L., Kuo, Y. C., Kuo, P. L., Ng, L. T., Kuo, Y.H., Lin, C. C. Apoptotic effects of
extract from Antrodia camphorate fruiting bodies in human hepatocellular
carcinoma cell lines. Cancer Letters, 221, 77-89, 2005.
Hseu, Y. C., Wu, F. Y., Wu, J. J. Anti-inflammatory potential of Antrodia camphorata
through inhibition of iNOS, COX-2 and cytokines via the NF-κB pathway.
International Immunopharmacology, 5, 1914-1925, 2005.
Hseu, Y. C., Yang, H. L., Lai, Y. C., Lin, J. G., Chen, G. W., Chang, Y. H. Induction of
apoptosis by Antrodia camphorata in human premyelocytic leukemia HL-60 cells.
Nutrition and Cancer. 48, 189-197, 2004.
Jurenka, J. Therapeutic Applications of Pomegranate (Punica granatum L.):A Review.
Alternative Medicine Review, 13, 128-144, 2008
Juan, M. Y., Chou, C. C. Enhancement of antioxidant activity, total phenolic and
flavonoid content of black soybeans by solid state fermentation with Bacillus
subtilis BCRC 14715. Food Microbiology, 27, 586-591, 2010.
Kaminogawa, S., Nanno, M. Modulation of immune functions by foods.
Evidence-Based Complementary and Alternative Medicine, 1, 241-250, 2005.
Kaplan, M., Hayek, T., Raz, A., Coleman R., Dornfeld L.,Vaya J., Aviram M.,
Pomegranate juice supplementation to atherotic mice reduces macrophage lipid
peroxidation, cellular cholesterol accumulation and development of atherosclerosis.
Journal of Nutrition, 131, 2082-2089, 2001.
Kokontis, J. M., Hay, N. Liao, S. Progression of LNCaP prostate tumor cells during
androgen deprivation : hormone-independent growth, repression of proliferation by
androgen, and role for p24Kip1 in androgen-induced cell cycle arrest. Mol
Endocrinol, 12,41-53, 1998.
Kim, S. J., Johnson, M., Koterba, K., Herynk, M. H., Uehara, H., Gallick, G. E.
Reduced c-Met expression by an adenovirus expressing a c-Met ribozyme inhibits
tumorigenic groeth and lymph node metastases of PC3-LN4 prostate tumor cells in
an orthotopic nude mouse model. Clinical Cancer Research, 9, 5161-5170, 2003.
Lee, I. H., Huang, R. L., Chen, C. T., Chen, H. C., Hsu, W. C., Lu, M. K. Antrodia
camphorata polysaccharides exhibit anti-hepatitis B virus effects.
FEMSMicrobiology Letters, 209, 63-67, 2002.
Lee, I. H., Hung, Y. H., Chou, C. C. Total phenolic and anthocyanin contents, as well as
antioxidant activity, of black bean koji fermented by Aspergillus awamori under
different culture conditions. Food Chemistry, 104, 936-942, 2007.
Lee, I. H., Hung Y. H., Chou C. C., Solid-state fermentation with fungi to enhance the
antioxidative activity, total phenolic and anthocyanin contents of black bean.
International Journal of Food Microbiology, 121, 150-156, 2008.
Lee, T. H., Lee, C. K., Tsou, W. L., Liu, S. Y., Kuo, M. T., Wen, W. C. A new cytotoxic
agent from solid-state fermented mycelium of Antrodia camphorata. Planta Medica,
73, 1412-1415, 2007.
Lee, Y., Yoon, J., Gunten, U. V. Spectrophotometric determination of ferrate (Fe(VI)) in
water by ABTS.Water Research, 39, 1946- 1953, 2005.
Liang, C. H., Syu, J. L., Mau, J. L. Antioxidant properties of solid-state fermented adlay
and rice by Phellinus linteus. Food Chemistry, 116, 841-845, 2009.
Lima, M. J. R., Tóth, I. V., Rangel, A. O. S. S., A new approach for the sequential
injection spectrophotometric determination of the total antioxidant activity. Talanta,
68, 207- 213, 2005.
Ling, S., Zhang, Z., Wang, H. Antioxidant activities of extracts and subfractions from
Inonotus obliquus. International Journal of Food Sciences and Nutrition, 60,
175-184, 2009.
Liu, D. Z., Liang, H. J., Chen, C. H. Comparative anti-inflammatory characterization of
wild fruiting body, liquidstate fermentation, and solid-state culture of
Taiwanofungus camphoratus in microglia and the mechanism of its action.
Journal of Ethnopharmacology, 113, 45-53, 2007b.
Liu, D. Z., Liang, Y. C., Lin, S. Y. l. Antihypertensive activities of a solid-state culture
of Taiwanofungus camphoratus (Chang-Chih) in spontaneously hypertensive rats.
Bioscience, Biotechnology and Biochemistry, 71, 23-30, 2007a.
Liu, F., Ooi, V. E.C. Chang S. T., Free radical scavenging activities of polysaccharide
extracts. Life Sciences, 60, 763-771, 1996.
Lu, M. K., Cheng, J. J., Lai, W. L., Lin, Y. R., Huang, N. K. Adenosine as an active
component of Antrodia cinnamomea that prevents rat PC12 cells from serum
deprivation-induced apoptosis through the activation of adenosine A2A receptors.
Life Sciences, 79, 252-258, 2006.
MacFarlane, M., Williams, A. C. Apoptosis and disease: a life or death decision. EMBO
reports, 5, 674-678, 2004.
Mau, J. L., Huang, P. N., Huang, S. J., Chen, C. C. Antioxidant properties of methanolic
extracts from two kinds of Antrodia camphorata mycelia. Food Chemistry, 86, 25-31,
2004.
McNeill, J., Barrie, F. R., Burdet, H. M., Demoulin, V., Hawksworth, D. L., Marhold, K.,
Nicolson, D. H., Prado, J., Silva, P. C., Skog, J. E., Wiersema, J. H., Turland N. J.,
International Code of Botanical Nomenclature (Vienna Code)., 2006.
Moor, G.E., Gerner, R. E., Franklin, H. A. Culture of Normal Human Leukocytes.
Journal of The American Podiatric Medical Association, 8,519-524, 1967.
Mosquera, O. M., Correa, Y. M., Buitrago, D. C., Niño, J. Antioxidant activity of twenty
five plants from Colombian biodiversity, Memórias do Instituto Oswaldo Cruz,
102, 631-634, 2007.
Nakamura N., Hirakawa A., Gao J.J. Five new maleic and succinic acid derivatives
from the mycelium of Antrodia camphorata and their cytotoxic effects on LLC
tumor cell line. Journal of Natural Products. 67, 46-48, 2004.
Okuda, R., Sone, Y., Wada, N., Kishida, E., Misaki, A. Structures and antitumor
activaties of the polysaccharides isolated from fruiting body and the growing
culture of mycelium of Ganoderma lucidum. Agricultural Biology and
Chemistry,49, 2641-2645, 1985.
Peng, C. C., Chen, K. C., Peng, R. Y., Chyau, C. C., Su, C.H. Antrodia camphorata
extract induces replicative senescence in superficial TCC, and inhibits the absolute
migration capability in invasive bladder carcinoma cells. Journal of
Ethnopharmacology, 109, 93-103, 2007.
Peng, C. C., Chen, K. C., Peng, R. Y., Su, C. H. Human urinary bladder cancer T24 cells
are susceptible to the Antrodia camphorata extracts. Cancer Letters, 243, 109-119,
2006.
Rao, Y. K., Fang, S.H., Tzeng, Y. M. Evaluation of the anti-inflammatory and
anti-proliferation tumoral cells activities of Antrodia camphorata, Cordyceps
sinensis, and Cinnamomum osmophloeum bark extracts. Journal of
Ethnopharmacology, 114, 78-85, 2007.
Sedej, I., Saka, M., Mandi, A, Mišan, A., Tumbas, V, Hadnaƌ ev, M. Assessment of
antioxidant activity and rheological properties of wheat and buckwheat milling
fractions. Journal of Cereal Science, 54, 347-353, 2011.
Shen, C. C., Kuo, Y. C., Huang, R. L. New ergostane and lanostane from Antrodia
camphorata. The Journal of Chinese Medicine,14, 247-58, 2003.
Shen, C. C., Yang, H. C., Huang, R. L., Chen, J. C., Chen, C.C. Anti-HBV principle
from the culture broth of Antrodia camphorata (strain # CCRC-35396). The
Journal of Chinese Medicine,16, 57-61, 2005.
Shen, Y. C., Yang, S. W., Lin, C. S., Chen, C. H., Kuo, Y. H., Chen, C. F. Zhankuic acid
F: a new metabolite from a formosan fungus Antrodia cinnamomea. Planta Medica,
6386-6388, 1997.
Shu, C. H., Lung, M. Y. Effect of culture pH on the antioxidant properties of Antrodia
camphorata in submerged culture. Journal of the Chinese Institute of Chemical
Engineers, 39, 1-8, 2008.
Song, T. Y., Hsu, S. L., Yeh, C. T., Yen, G. C. Mycelia from Antrodia camphorata in
submerged culture induced apoptosis of human heptoma Hep G2 cells possibility
through regulation of Fas pathway. Journal of Agricultural and Food Chemistry, 13,
5559-5564, 2005.
Song, T. Y., Hsu, S. L., Yen, G. C. Induction of apoptosis in human hepatoma cells by
mycelia of Antrodia camphorate in submerged culture Journal of Sone, 100,
15-167, 2005.
Song, T. Y., Yen, G. C. Antioxidant properties of Antrodia camphorata in submerged
culture. Journal of Agricultural and Food Chemistry, 50, 3322-3327, 2002.
Song, T. Y., Yen, G. C. Protective effects of fermented filtrate from Antrodia camphorata
in submerged culture against CCl4-induced hepatic toxicity in rats. Journal of
Agricultural and Food Chemistry, 51, 1571-1577, 2003.
Tsai, Z. T., Liaw, S. L. The Use and the Effect of Ganoderma, San Yun Press, Taichung,
Taiwan, 1985.
Tu, Y., Du, J.R., Wang, C.Y. protection against hydrogen peroxide-induced injury by
Z-ligustilide in PC-12 cells. Experimental Brain Research, 184, 307-312, 2008.
Valkoa, M., Leibfritzb, D., Moncol, J., Cronin, M. T. D., Mazur, M., Telser, J. Free
radicals and antioxidants in normal physiological functions and human disease.
The International Journal of Biochemistry & Cell Biology, 39, 44-84, 2006.
Vojdani , A., Erde , J. Regulatory T cells, a potent immunoregulatory target for CAM
researchers: modulating tumor immunity, autoimmunity and alloreactive immunity
(III). Evidence-Based Complementary and Alternative Medicine, 3, 309-316, 2006.
Waris, G., Ahsan, H. Reactive oxygen species: role in the development of cancer and
various chronic conditions. Journal of Carcinogenesis, 5, 14, 2006.
Wasser, S. P., Weis, A. L. Therapeutic effects of substances occurring on higher
basidomycetes mushrooms : a modern perspectives. Critical Reviews in
Immunology, 19, 65-96, 1999.
Wu, D. P., Chiang, C. H. Constituents of Antrodia cinnamomea. Journal of the Chinese
Chemical Society, 797-800, 1995.
Wu, H., Pan, C. L., Yao, Y. C., Chang, S. S., Li, S. L., Wu, T.-F. Proteomic analysis of
the effect of Antrodia camphorate extract on human lung cancer A549 cell.
Proteomics, 6, 826-835, 2006.
Wu, M. D., Cheng, M. J., Wang, B. C., Wang, W. Y., Lai, J. T., Yuan, G. F. Chemical
constituents from the mycelia of Antrodia cinnamomea. Journal of the Chilean
Chemical Society, 52, 1338-1340, 2007
Wu, M. D., Cheng, M. J., Wang, B. C. Maleimide and maleic anhydride derivatives
from the mycelia of Antrodia cinnamomea and their nitric oxide inhibitory
activities in macrophages. Journal of Natural Products, 71, 1258-1261, 2008.
Wu, S.H., Ryvarden L., Chang T.T. Antrodia camphorata (“niu-chang-chih”), new
combination of a medicinal fungus in Taiwan. Botanical Bulletinof Academia
Sinica, 38, 273-275, 1997.
Yang, S. S., Wang, G. J., Wang, S. Y., Lin, Y. Y., Kuo, Y. H., Lee, T. H. New constituents
with iNOS inhibitory activity from mycelium of Antrodia camphorata. Planta
Medica, 75, 512-516, 2009.
Yang, S. W., Shen, Y. C., Chen, C. H. Steroids and triterpenoids of Antrodia
cinnamomea-a fungus parasitic on Cinnamomum micranthum. Phytochemistry, 41,
1389-1392, 1996.
Yu, Z. H., Wu, S. H., Wang, D. M., Chen, C. T. Phylogenetic relationships of Antrodia
species and related taxa based on analyses of nuclear large subunit ribosomal DNA
sequences. Botanical Studies, 51, 53-60, 2010.
Zang, M., Su, Q. Ganoderma comphoratum, a new taxon in genus Ganoderma from
Taiwan, China. Acta Botanica Yunnanica, 12, 395-396, 1990.
陳勁初、林文鑫、陳清農、許勝傑、黃仕政、陳炎鍊,「台灣特有真菌- 樟芝菌絲
體之開發」. Fung Science, 16, 7-22, 2001.
陳良孙, 鄭建瑋, 王志玄, 林志樟, 張云力, 李瑞玲, 游欣, 梁致遠, 鹼催化對
Folin-Ciocalteu 詴劑檢測總多酚含量的影響, 銘傳大學健康科技學院生物科技
學系, 4, 10-19, 2012.
李和俊, 前列腺癌之蛋白質表現分析,中山大學生物醫學研究所, 2004.
時磊, 總抗氧化能力、總酚測定方法的優化及在藍莓葡萄乾紅酒釀造中的應用研究,
煙台大學, 2010.
蔡碧芬, 山葵萃取物抗氧化能力的探討, 中山醫學大學生化暨生物科技研究所碩
士論文, 2010.
羅郁中, 台灣六種野生植物果實之抗氧化功能評估, 中央研究院高中生命科學資
優生培育計畫專題研究報告, 2003.
姜淑繡, 省產蘿蔔之抗氧化性研究, 大葉大學食品工程學系碩士班碩士論文, 2001.
劉怡君, 分析六種唇型花科植物之迷迭香酸含量及其抗氧化能力, 生物工程研究
所碩士論文, 2006.
林怡菁, 加熱處理對黑豆麴抗氧化活性與總酚類化合物及花青素含量之影響, 國
立台灣大學食品科技研究所, 2006.
郝功元, 銀杏花粉多醣的提取分離及抗氧化性的研究, 南京林業大學, 2009.
謝昆霖, 番木瓜果實水萃取物之抗氧化能力研究, 靜宜大學食品營養學系碩士論
文, 2007.
姚躍飛, 曾柏全, 現代固態發酵技術在食品加工業中的應用, 食品與機械, 21,
89-92, 2005.
朱海文, 牛樟芝椴木培養之研究探討, 朝陽科技大學應用化學系, 2005.
周佳賢, 蛹蟲草固態培養研究探討, 朝陽科技大學生物技術研究所, 2005.
林冠廷, 影響蛹蟲草固態發酵生產蟲草素之研究, 國立宜蘭大學生物技術研究所
碩士論文, 2010.
吳彩平, 以固態發酵製備樟芝米及其品質與抗氧化性質, 國立中興大學食品暨應
用生物科技學系碩士論文, 2006.
王柏森, 樟芝(Antrodia cinnamomea)固態發酵培養產物之成分分析與抗氧化活性之
研究, 國立屏東科技大學生物科技研究所碩士學位論文, 2008.
賴尚志, 三點金草抗氧化及鎮痛抗發炎研究, 中國醫藥大學博士論文, 2010.
蔡孟勳, 探討通氣量對於樟芝發酵生產抗氧化物質之影響, 國立中央大學化學工
程與材料工程研究所碩士論文, 2004.
孫璐西, 龍眼花萃取物抗氧化性之探討, 國立臺灣大學食品科技研究所碩士論文,
2005.
葉展均, 以固態發酵製備白樺茸胚芽米及其呈味性質與生理活性, 中興大學碩士
論文, 2009.
吳艾真, 桑椹浸漬萃取條件對成分溶出及萃取液抗氧化能力之影響, 國立臺灣大
學生物資源暨農學院園藝所碩士論文, 2007.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊