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研究生:Wira Winardi
研究生(外文):Wira Winardi
論文名稱:靈芝蛋白8 (LZ-8)對小鼠皮膚傷口癒合之評估:調節嗜中性白血球、角質細胞和纖維母細胞
論文名稱(外文):Characterization of Protein LZ-8-Induced Wound Healing Acceleration on Mouse Skin: Emphasizing in Modulation of Neutrophils, Keratinocytes, and Fibroblasts
指導教授:張榮善
指導教授(外文):Jung-Shan Chang , PhD
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:醫學科學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:英文
論文頁數:63
外文關鍵詞:KeratinocyteWound healingPMN cell infiltrationCollagenFibroblasts
相關次數:
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The benefit of LZ-8 as immunomodulatory protein has been widely studied. LZ-8 reported might accelerate rat liver wound injury. However, the effect of LZ-8 on skin wound healing is less studied.This study aimed to evaluate the effectsof LZ-8 in mouse skin wound healing and determine the molecular mechanism of LZ-8-induced acceleration of wound healing.Different group of wound were generated at C57Bl/6 mice dorsal skin followed by wound histology observation. To evaluate the effect of LZ-8 for fibroblast and keratinocyte migration we performed In Vitro wound healing assay.Our studied revealed that an increase in PMN infiltrations and collagen depositions in the wound of animals treated with LZ-8 on day 2 and day 3 respectively. In addition,we found that both fibroblast and keratinocyte migration were significantly increase under treatment with LZ-8In Vitro study of wound healing. These results suggested that LZ-8 not only modulate PMN cell infiltration, but also play an active role in enhancing fibroblast and keratinocyte migration and increase collagen deposition.

Table of Content
Table of content i
List of figures iii
Abstract v
Introduction 1
1.1 Skin Anatomy and Physiology 2
1.1.1 Epidermis Surface of the Skin 2
1.1.1.1 Stratum Basale 3
1.1.1.2 Stratum Spinosum 3
1.1.1.3 Stratum Granulosum 3
1.1.1.1 Stratum Corneum 4
1.1.2 Dermis Surface of the Skin 4
1.2 Skin Wound and Types of Wound 5
1.3 Skin Wound Repair and Regeneration 6
1.3.1 Hemostasis and Coagulation Phase/ Lag Phase 6
1.3.2 Inflammation Phase 7
1.3.2.1 Neutrophil Role in Wound Healing 7
1.3.2.1 Macrophage Role in Wound Healing 8
1.3.1 Hemostasis and Coagulation Phase/ Lag Phase 8
1.3.3 Proliferation and Repair Phase 9
1.3.3.1 Fibroblast 10
1.3.3.2 Keratinocyte 11
1.3.4 Skin Remodeling 14
1.4 Ling Zhi-8 14
1.5 Aim of the Study 16
Experimental Section 17
Results 26
Discussion 30
Conclusion 34
Future Work 35
Reference 58


Reference


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