臺灣博碩士論文加值系統

本論文是利用二種螢光染料的螢光共振能量轉移（Fluorescence
resonance energy transfer，FRET)探討聚合物微胞體（polymeric micelles），在可能影響其穩定性的因子：時間、存在環境、機械影響震盪三種存在下，進行聚合物微胞體的穩定評估。此外，亦利用FRET評估不同聚合物對於能量轉移的效益優劣。In vitro的部分，則是將聚合物微胞體與二種不同的螢光染料混合，經由Franz Diffusion Cell System觀察在穿皮的效益。
由實驗結果得知在0～8小時內，聚合物微胞體與螢光共振能量轉移比值，都能保持穩定的狀態；聚合物微胞體在不同稀釋濃度的環境下，其螢光共振能量轉移比值會隨著存在環境而受到影響；聚合物微胞體在受到機械震盪的影響下，其螢光共振能量轉移比值，仍能保持穩定的狀態。但在不同的聚合物微胞體，在不同濃度比例的螢光染料存在下，其螢光共振能量轉移則會有所不同。0～24小時的Franz Diffusion Cell System實驗，可以發現混合二種螢光染料的載體，能有效進行的穿皮。

The study used two fluorescence dyes’ Fluorescence resonance energy transfer (FRET) to evaluate the stability of polymeric micelles by timing, environment, and mechanical shake.Moreover, we also use FRET to evaluate pros and cons of different polymers’ energy transfer. In vitro, we formulated polymeric micelles and two fluorescence dyes and observed the transdermal permeation by Franz Diffusion Cell System.
The results found that polymeric micelles and two fluorescence dyes’ ratio can sustain stable during 0～8 hours. Polymeric micelles and two fluorescence dyes’ ratio we affected under concentration of different dilutions, and the ratio could maintain stable mechanical under shaking. However, the different polymeric micelles’ FRET was different under different concentration fluorescence dyes.During 0～24 hours of Franz Diffusion Cell System’s experiment, it were found that two fluorescence dyes’ could transdermal penetration.

目錄……………………………………………………………………………………3
附表目錄………………………………………………………………………………4
附圖目錄………………………………………………………………………………5
中文摘要………………………………………………………………………………7
英文摘要………………………………………………………………………………8
壹、緒論………………………………………………………………………………9
一、皮膚結構………………………………………………………………………9
二、經皮吸收的優點與阻礙………………………………………………………15
三、經皮吸收的途徑………………………………………………………………16
四、奈米粒子的藥物載體系統……………………………………………………18
五、利用螢光染料進行研究………………………………………………………21
貳、研究目的…………………………………………………………………………26
參、材料與研究方法…………………………………………………………………27
一、實驗試藥………………………………………………………………………27
二、實驗儀器………………………………………………………………………27
三、溶液、試劑製備………………………………………………………………28
四、實驗動物………………………………………………………………………30
五、研究方法………………………………………………………………………30
肆、結果與討論………………………………………………………………………39
1.Polymer（PM）的臨界微胞體濃度（Critical micelle concentration
CMC）……………………………………………………………………………39
2.螢光聚合物微胞體大小測定…………………………………………………49
3.二種螢光染料的光譜圖………………………………………………………52
4.聚合物微胞體的穩定性………………………………………………………54
5.環境的稀釋作用對於聚合物微胞體穩定性的影響…………………………57
6.機械性震盪作用對於聚合物微胞體安定性的影響…………………………60
7.不同種類5％聚合物微胞體所產生的Forster resonance energy transfer（FRET）效應……………………………………………………………………61
8.螢光聚合物微胞體的血液毒性（hemotoxicity）實驗………………………65
9.螢光聚合物微胞體穿皮實驗…………………………………………………66
伍、結論………………………………………………………………………………69
參考文獻……………………………………………………………………………70

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