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研究生:盧姿妤
研究生(外文):Tze-Yu Lu
論文名稱:探討酸鹼值對於光感色素安定性之影響與應用
論文名稱(外文):Investigation of the pH Effect on the Stability and Application of the Photosensitive Dye
指導教授:蔡翠敏
指導教授(外文):Tsuimin Tsai
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:生醫材料暨組織工程研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:72
中文關鍵詞:牙菌斑顯示劑赤蘚紅牙菌斑pH值光動力作用
外文關鍵詞:Plaque disclosing agentErythrosinePlaquepHPhotodynamic action
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牙周病和蛀牙是口腔內常見的兩大口腔疾病,其罹患的原因多為口腔清潔不完善而造成細菌生物膜及牙菌斑的孳生。為了改善口腔衛生習慣,臨床上已開發一種能簡易檢測牙菌斑分佈情形的試劑,利用赤蘚紅 (Erythrosine,ER) 可吸附於細胞表面使得牙菌斑染色的特性,市面上作為牙菌斑顯示劑已被廣泛的使用,亦有研究指出赤蘚紅也能作為光感色素,結合光動力作用來抗菌。然而,赤蘚紅不僅會染色牙菌斑,還會沾染於牙齦、嘴唇等軟組織長達數小時難以去除,因此如果能於診斷時或光動力治療後,以快速的方式清除可見的紅色染斑,更能有效增加病患對此種診斷方式及治療的意願。而本研究發現,赤蘚紅會因為暴露於酸性溶液內,由原本的鮮紅色進而有變淡的情況,並從調整溶液的酸鹼值和控制照光的時間,得知酸性溶液亦會影響赤蘚紅之吸收光譜與光動力作用的能力。

Periodontal disease and tooth decay are two common oral diseases. These two diseases are closely related to inadequate oral hygiene that results in formation of bacterial biofilm and even tooth plague. In order to improve the public oral hygiene, an easy method to detect oral plague has been developed. Erythrosine (ER), a red color dye, is able to stain the bacterial biofilm in the oral cavity, and has been widely used as a plaque disclosing agent. ER has also been served as a photosensitizer used for photodynamic anti-bacterial treatment. However, erythrosine stains not only the plague but also the soft tissues such as gingiva and lips into red for hours. Therefore, quickly removal of the color after visualizing the plaque or conducting the photodynamic treatment may increase patients’ compliance of such diagnosis and dental treatment. In this study, we found that the persisting bright red color of ER is subjected to photobleaching, and may also be removed by exposing it to lower pH solution. Solution pH and light exposure time were found to affect the spectral and photodynamic properties of ER.

摘要 ............................................. i
Abstract ......................................... ii
目錄 ............................................. iii
圖索引............................................ vi
表索引............................................ viii
第一章 前言....................................... 1
1-1 光動力作用 ................................... 1
1-1.1 光動力原理 ................................. 2
1-1.2 光動力應用 ................................. 3
1-2 牙菌斑 ....................................... 6
1-2-1牙菌斑的去除 ................................ 7
1-2-2牙菌斑顯示劑 ................................ 8
第二章 研究目的與動機............................. 9
第三章 文獻探討................................... 11
3-1 赤蘚紅之介紹 ................................. 11
3-1.1赤蘚紅結構特性 .............................. 11
3-1.2赤蘚紅應用 .................................. 12
3-1.3赤蘚紅之光動力抗菌........................... 13
3-2 玫瑰紅 ....................................... 14
3-3 竹莢桃紅 ..................................... 15
3-4 光源 ......................................... 16
3-5 緩衝溶液 ..................................... 16
3-6 光反應速率 ................................... 17
3-7 單態氧檢測試劑 ............................... 17
3-8 自由基捕捉劑 ................................. 19
第四章 材料與方法 ................................ 20
4-1 材料 ......................................... 20
4-2 儀器 ......................................... 21
4-3 實驗方法 ..................................... 22
4-3.1溶液配製 .................................... 23
4-3.2赤蘚紅之濃度曲線 ............................ 23
4-3.3赤蘚紅之光反應速率 .......................... 23
4-3.4酸鹼值環境對光感色素之影響 .................. 24
4-3.5單態氧測試 .................................. 25
4-3.6自由基訊號強度分析 .......................... 27
4-3.7紅外線光譜結構分析 .......................... 27
第五章 實驗結果與討論............................. 28
5-1 赤蘚紅之最佳濃度 ............................. 28
5-2 赤蘚紅之光漂白現象 ........................... 28
5-3 赤蘚紅之光反應速率 ........................... 29
5-4 酸鹼值對光感色素之影響 ....................... 30
5-5 單態氧測試 ................................... 32
5-6 自由基之產生 ................................. 36
5-7 紅外線光譜之結構分析 ......................... 37
第六章 結論 ...................................... 38
第七章 附圖表 .................................... 39
第八章 文獻參考................................... 56

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