臺灣博碩士論文加值系統

臺灣是全世界龍眼(longan)主要產區之一，龍眼在食品應用上，其中一部分可供鮮食，另一部分則是經去核加工製成桂圓。芒果(mango)主要生長於熱帶地區，亦為臺灣特有多產之水果。但無論龍眼或芒果，經由鮮食或食品加工後，將產生大量廢棄龍眼核(longan seed)與芒果皮(mango peel)。文獻指出：龍眼核與芒果皮，皆內含大量植物多酚類化合物，如鞣花酸(Ellagicacid, EA)、鞣花單寧(Ellagitinnins, ETS)及沒食子酸(Gallic acid, GA)。因此，若能自廢棄龍眼核與芒果皮，萃取回收鞣花酸，將符合廢棄物回收利用及經濟效益提升之雙重目的。
鞣花酸主要存在於植物中的多酚類化合物，在植物可自體生成鞣花酸並將其轉化為鞣花單寧、沒食子酸或其他衍生物。文獻指出：鞣花酸可應用於化妝品之美白及醫藥方面之抗氧化、抗癌、抗突變及消炎等。因此，本研究擬從廢棄龍眼核與芒果皮中直接萃取鞣花酸，並探討提升其萃取效率之相關萃取製程，若能自廢棄之龍眼核與芒果皮，大量萃取並回收鞣花酸，預期鞣花酸在化妝品及醫藥業應用上應具備良好的商業化開發潛力。
本研究首先探討不同萃取方式對龍眼核與芒果皮回收鞣花酸之影響，分別以萃取粉末1g萃取溶劑10ml固液比例1: 10進行研究探討，研究結果顯示，龍眼核最佳之萃取條件為：以55%(v/v)乙醇溶劑，在轉速200 rpm，萃取時間2小時，溫度30℃下可回收最高之鞣花酸達276 mg/L、鞣花單寧567 mg/L、沒食子酸434 mg/L。其次，在芒果皮回收鞣花酸之最佳之萃取條件則為：以95%(v/v)乙醇溶劑，在轉速200 rpm，萃取時間2小時，溫度30℃下可回收最高之鞣花酸達225 mg/L、沒食子酸138 mg/L。未來本研究將持續針對鞣花酸於化妝品及醫藥產業應用上，進行相關之分析評估。

Ellagic acid (EA) is a polyphenolic compounds (PPCs) found in fruits and berries, such as longan, mango, pomegranates, strawberries, raspberries, and blackberries and has been found to have antioxidant, anticarcinogenic, antifibrosis, and chemopreventive activities. From a chemical point of view, PPCs can react with one-electron oxidants, which prevent free radical formation in biological systems. Therefore, the aim of this study is to focus on the extraction of EA from longan seed and mango peel. It was found that using 1/10 (w/w) of solid-liquid ratio and 55% (v/v) of ethanol in 2 h at 30℃ and 200 rpm agitation could attain better EA concentration 276 mg/L for longan seed. Moreover, the optimal extraction conditions were determined for mango peel, giving an EA extraction yield of 361 mg/L and 95% (v/v) of ethanol in 2 h at 30℃ and 200 rpm agitation. The results obtained from this work could provide valuable information for future extraction process development toward the commercialization of the ellagic acid.

書名頁 I
推薦函 II
審定書 III
中文摘要 IV
英文摘要 VI
誌謝 VII
目錄 VIII
表目錄 XI
圖目錄 XII
第一章、緒論 1
1.1前言 1
1.2 研究動機與目的 2
第二章、文獻回顧 3
2.1單寧酸 3
2.2 鞣花酸 6
2.3 鞣花單寧 7
2.4 鞣花酸及鞣花單寧應用 8
2.5 龍眼 10
2.5.1 龍眼核的化學成分 13
2.6 芒果 14
2.7 多酚化合物 18
第三章、材料與方法 20
3.1 實驗材料 20
3.1.1 實驗設備儀器 20
3.1.2 實驗化學藥品 21
3.2 實驗方法 22
3.2.1 萃取樣品 23
3.2.2 萃取流程 23
3.2.3 萃取步驟 23
3.2.4 龍眼核與芒果皮粉末萃取 24
3.3 高效液相層析儀(HPLC)分析 30
第四章、結果與討論 31
4.1 標準曲線 31
4.2 萃取龍眼與芒果初步之影響 37
4.3 萃取條件之影響 38
4.4 萃取龍眼核之影響 41
4.5 萃取芒果皮之影響 45
4.6 不同方式萃取龍眼核之探討 47
4.7 不同方式萃取芒果皮之探討 50
第五章、結論與未來展望 53
參考文獻 55

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Workshop-seminar"Makingbetter use of local feed resources"
MEKARN-CTU, Cantho, 23-25 May, 2005
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