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研究生:陳迦陵
研究生(外文):Chia-Ling Chen
論文名稱:台灣農產品加值在化粧品上之應用
論文名稱(外文):Application of Taiwanese agricultural produce value-added on cosmetics
指導教授:陳榮秀陳榮秀引用關係
指導教授(外文):Zong-Shiow Chen
口試委員:林清宮黃昭菱
口試委員(外文):Ching-Gong LinJau-Ling Huang
口試日期:2015-07-30
學位類別:碩士
校院名稱:嘉南藥理大學
系所名稱:化粧品應用與管理系
學門:民生學門
學類:美容學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:135
中文關鍵詞:抑制酪胺酸酶質體DNA保護抗氧化美白複方萃取物
外文關鍵詞:in vitro inhibition of tyrosinaseplasmid DNA protectionantioxidantwhiteningcomposite extracts
相關次數:
  • 被引用被引用:1
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  • 下載下載:24
  • 收藏至我的研究室書目清單書目收藏:2
台灣農產品蓬勃發展,許多農產品經過加工製程所產生的廢棄物,通常都被當垃圾處理掉,本篇研究主要以環保回收再利用的綠色產品為概念,選取了六種萃取物,萃取植物為不同品種的米糠、粗糠,荔枝皮、荔枝種子,上述植物以水為溶劑,以加熱迴流方式萃取、冷凍乾燥,進行其抗氧化能力、美白、總酚含量、細胞毒性、DNA保護率、UV吸收光測定等一系列的活性評估試驗。
結果顯示DPPH˙自由基清除率最佳者為荔枝種子萃取物,其SC50為0.064 mg/mL,其在1 mg/mL的濃度時體外酪胺酸酶抑制率為21.34% ;荔枝皮萃取物 DNA保護率在2.5 mg/mL的濃度為95.34%;粗糠萃取物之ABTS˙+ 自由基清除率SC50為0.020 mg/mL,且具有抑制痤瘡桿菌及皮屑芽孢菌作用;米糠總酚含量為115.22 mg GAE/g。
將美白及抑菌活性較明顯之粗糠、荔枝皮、荔枝種子萃取物以 1 : 1 : 1配製成複方萃取物M並評估其活性。結果顯示其ABTS˙+ 自由基清除率具有提升作用,其體外酪胺酸酶抑制率明顯提升為33.60±1.72 %。最後進行配方設計,將複方萃取物 M添加至配方中,評估產品穩定性,功效性評估,結果顯示其具有美白、保濕、減少斑點、撫平細紋、降低肌膚敏感、及減少紫質生長等功效。此研究成果在環保意識抬頭的趨勢下,在未來能讓更多的農產品廢棄物得以利用,除了能降低成本外還能兼具環保,創造出農產品新的商業價值。

Vigorous development of Taiwan's agricultural , a lot of agricultural products are processed waste generated by the process, usually it just like a garbage treated discard , this study in an environmentally green products recycling concept, selected six extracts, plant extracts codenamed as chaff, rice bran of different varieties , litchi rind, litchi seeds .The plant with water as the solvent, dried and frozen after extraction was heated to reflux, and then assessment of extract activity. Including antioxidant capacity, whitening capacity, total phenols test, MTT assay, plasmid DNA protection test, UV-Vis spectrometer test ,activity test of series.
The results showed that water extract of litchi seeds radical DPPH˙ (SC50:0.064 mg / mL), in vitro inhibition of tyrosinase activity, at a concentration of 1 mg / mL was 21.34% ; extract litchi rind DNA protection rate of 2.5 mg / mL, was 95.34%;DNA protection test of 2.5 mg / mL of concentration of 95.34%; extract chaff ABTS˙ radical scavenging (SC50: 0.020 mg / mL), and inhibit acne bacteria and Bacillus dander role; extract rice bran total phenol test (115.22 mg GAE / g).
Select whitening and antibacterial activity is better of the litchi rind, litchi seeds extract proportion by 1: 1: 1 extract formulated as a compound M and evaluate it’s activity. Results showed that ABTS˙ + radical scavenging has elevated the role, its in vitro tyrosinase inhibition rate was promoted to 33.60 ± 1.72%. Finally, the formulation design, add compound extraction M to the formulation, assessment of product stability, efficacy assessment, the results show it has whitening, moisturizing, reducing spots, fine lines, reduce skin sensitivity, and reduce the growth of rhodopsin and other effects . This research results in the rise of environmental awareness trend in the future to make more use of agricultural waste, in addition to reducing the external costs of environmental protection can both create the new commercial value.

中文摘要 I
Abstract III
誌謝 IV
目錄 V
表目錄 VIII
圖目錄 XI
第一章 序論 1
1.1前言 1
1.2研究動機與目的 2
第二章 文獻回顧 3
2.1 植物介紹 3
2.1.1米糠、粗糠 3
2.1.2荔枝 5
2.2 自由基的介紹 7
2.2.1自由基定義 7
2.2.2自由基、活性氧所造成生物體之危害 8
2.3美白介紹 9
2.3.1 黑色素之生成 9
2.3.2 黑色素之相關酵素 9
第三章 材料與方法 11
3.1實驗架構 11
3.2實驗材料 12
3.2.1儀器與設備 12
3.2.2 實驗藥品 13
3.2.3 實驗菌種與細胞 14
3.2.4產品配方成分 15
3.3實驗材料製備 16
3.3.1 天然物萃取 16
3.3.2 天然物萃取方法及冷凍乾燥 16
3.3.3縮寫表 17
3.4活性試驗 18
3.4.1 DPPH˙自由基清除試驗 18
3.4.2 ABTS˙+ 陽自由基清除率試驗 20
3.4.3體外酪胺酸酶抑制試驗 22
3.4.4 DNA電泳評估OH˙所引發DNA損傷之保護作用 24
3.4.5總酚類含量測定 27
3.4.6 B16老鼠黑色素細胞瘤釋放黑色素測定 29
3.4.7 3T3細胞毒性試驗 31
3.4.8抗菌活性試驗 33
3.5 紫外線吸收光譜測定 34
3.6 天然物萃取液應用於化粧品 35
3.6.1 產品配方設計 35
3.6.2 產品外觀變化 37
3.6.3 產品黏度變化 37
3.6.4 產品酸鹼度變化 37
第四章 實驗結果 38
4.1天然萃取物之產率 38
4.2 DPPH˙自由基清除試驗結果 39
4.3 體外酪胺酸酶抑制試驗結果 41
4.4 ABTS˙+ 陽離子自由基清除試驗結果 44
4.5總酚類含量測定結果 46
4.6 抗菌活性試驗結果 49
4.7 UV吸收光譜測定 55
4.8 複方活性測試結果 58
4.8.1 複方DPPH ˙自由基清除率結果 58
4.8.2複方體外酪胺酸酶抑制率結果 59
4.8.3 複方ABTS˙+ 陽離子自由基清除率結果 60
4.8.4複方總酚類含量結果 61
4.8.5 pUC 119 DNA保護試驗結果 62
4.8.6 B16老鼠黑色素細胞瘤釋放黑色素測定 66
4.8.7 3T3細胞毒性試驗結果 68
4.8.8 複方抗菌活性試驗結果 70
4.9 產品安定性試驗 74
4.9.1產品外觀變化 74
4.9.2產品酸鹼值測定 85
4.9.3產品黏度變化 90
4.10產品功效性評估 95
4.10.1產品美白評估 95
4.10.2產品保濕評估 101
4.10.3全臉膚質功效評估 103
第五章 結論 123
第六章 參考文獻 130



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