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研究生:薛方眉
研究生(外文):SYUE,FANG-MEI
論文名稱:台灣溫泉於化妝品應用功效之初評
論文名稱(外文):Efficiency Evaluation of Taiwan Hot Spring on Cosmetic Applications
指導教授:甘其銓甘其銓引用關係
指導教授(外文):GAN,CI-CYUAN
口試委員:許澤宇楊惠玲林清宮萬孟瑋
口試委員(外文):SYU,ZE-YUYANG,HUEI-LINGLIN,CING-GONGWAN,MENG-WEI
口試日期:2014-07-25
學位類別:碩士
校院名稱:嘉南藥理大學
系所名稱:觀光事業管理系
學門:民生學門
學類:觀光休閒學類
論文種類:學術論文
論文出版年:2014
畢業學年度:103
語文別:中文
論文頁數:128
中文關鍵詞:溫泉人體有效性評估抑菌能力  嗜熱桿菌
外文關鍵詞:Hot Springsthermophilesfacial masksbody's assessment of the effectivenessthe antibacterial capability
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本實驗特別選用各具特色的五種溫泉水分別為酸性硫酸鹽氯化物青磺泉(青磺)、酸性硫酸鹽白磺泉(白磺)、氯化物碳酸氫鈉含鐵泉(瑞穗)、中性硫酸鹽氯化物泉(安通)、碳酸氫鈉泉(龜丹)等來做研究,評估其抗氧化能力、自由基清除率、人體有有效性評估(美白、粗糙度、保濕、彈性、紅色區、紫外線色斑、棕色斑、紫質、毛孔、斑點)、抑菌能力之評估等活性試驗。
在抗氧化能力最好,清除自由基效果最高的為中性硫酸鹽氯化物泉(安通),而在抑菌活性中效果最好的為在濃度0.1mg/0.5mL時,酸性硫酸鹽氯化物青磺泉(青磺)對於酵母菌的抑菌能力最好抑菌圈為30±0.21mm屬於高度抑菌活性,產品配方設計中將五種溫泉水放製面膜液配方中之後做人體有效性評估,其中美白效果最好的為碳酸氫鹽泉(龜丹),改善皮膚粗糙度最好的為中性硫酸鹽氯化物泉(安通),可以改善皮膚表面彈性的為中性硫酸鹽氯化物泉(安通),可以增加皮膚保濕度的為碳酸氫鹽泉(龜丹),可以改善毛孔的泉質又可分為全臉測試中抑制毛孔效果最好的為中性硫酸鹽氯化物泉(安通)及局部測試中具有抑制效果的為碳酸氫鹽泉(龜丹),抑制紫外線色斑效果最好的為中性硫酸鹽氯化物泉(安通),對於皮膚具有安撫鎮定效果的為酸性硫酸鹽氯化物青磺泉(青磺),能抑制斑點生成率的泉質為酸性硫酸鹽氯化物青磺泉(青磺),五種面膜測試下來最不液生成棕色斑的泉質為碳酸氫鹽泉(龜丹),能抑制紫質的泉質為中性硫酸鹽氯化物泉(安通)。
故可以根據以上五種溫泉水測試的結果,再依不同的膚質設計出不同的配方來應用於化妝品保養中。
This experiment selected five kinds of hot spring water, including acid sulfate chlorides green sulfonic springs (green sulfonic), acid sulfate white sulfonic springs (white sulfonic), chlorides sodium bicarbonate containing chalybeate (Rueisuei), neutral sulfate chlorides springs (An Tong), and sodium bicarbonate springs (Gui Dan). These hot spring waters are chosen to evaluate anti-oxidation capacity, free radical removal, casein decomposition capacity, human effectiveness assessment (whitening, roughness degrees, moisturizing, elasticity, red area, UV colored spots, brown spots, porphyrin, pores, and spots), and antibacterial ability.
In the anti-oxidation capac¬ity evaluation, neutral sulfate chlorides springs (An Tong) showed the best performance in in free radical removal. Green sulfonic 6th bacteria has the strongest casein decomposition capacity with concentration of 0.1 mg/ 0.5 mL. Acid sulfate chlorides green sulfonic springs (green sulfonic) has highest bacteriostatic ability for yeasts when inhibition bacteria circle is 30 ± 0.21mm, which is high bacteriostatic ability. Five kinds of hot spring water were used as the ingredients in facial masks to evaluate the human effectiveness assessment. Sodium bicarbonate springs (Gui Dan) has the best whitening and moisturizing effect. Neutral sulfate chlorides springs (An Tong) has the best effect in skin roughness, elasticity improvement and UV colored spot inhibition. Neutral sulfate chlorides springs (An Tong) has the best effect in pore improvement, and sodium bicarbonate springs (Gui Dan) also has effect in partial testing. Acid sulfate chlorides green sulfonic springs (green sulfonic) can inhibit spots generation and stabilize skin condition. Among the five facial masks, sodium bicarbonate springs (Gui Dan) could generate the least brown spots, and neutral sulfate chlorides springs (An Tong) can inhibit porphyrin accumulation.
According to the experimental result of five kinds of hot springs water, different formula could be designed for various skin in cosmetics.
中文摘要............I
Abstract.......III
目錄.......V
圖目錄.......VIII
表目錄 .......XI
第一章 緒論.......1
1-1研究動機.......1
1-2 研究目的.......2
第二章 文獻回顧.......3
2-1溫泉的形成與標準.......3
2-1-1 溫泉的形成.......3
2-1-2溫泉標準.......4
2-1-3 溫泉泉質檢測技術.......5
2-1-4 本實驗研究之泉質.......8
2-2 容易造成皮膚生理感染及化妝品製造中常見的菌種 .......9
2-2-1綠膿桿菌(Pseudomonas aeruginosa).......9
2-2-2大腸桿菌(Escherichia coil).......9
2-2-3金黃色葡萄球菌(staphylococcus aureus).......10
2-2-4痤瘡桿菌(Propionibacterium acnes).......11
2-2-5酵母菌(Yeasts).......11
2-3皮膚的構造.......13
2-3-1 皮膚表面.......13
2-3-2 皮膚內部構造.......14
2-4 嗜熱桿菌.......17
第三章 材料與方法.......18
3-1 實驗架構.......18
3-2 實驗儀器與設備.......19
3-2-1 實驗儀器.......19
3-2-2 實驗藥品.......20
3-2-3 實驗菌種.......21
3-2-4產品配方與成分....... 21
3-3實驗製備與方法.......22
3-3-1菌種培養.......22
3-3-1-1培養基之製備....... 22
3-3-1-2培養液之製備....... 23
3-3-1-3菌種活化與保存.......24
菌種.......25
培養基.......25
培養條件.......25
痤瘡桿菌.......25
金黃色葡萄球菌.......25
綠膿桿菌.......25
大腸桿菌.......25
酵母菌.......25
皮屑芽孢菌.......25
3-4-1DPPH .自由基清除試驗.......26
3-4-2 ABTS.+總抗氧化能力測定.......29
3-5 溫泉水抗菌評估試驗.......32
3-5-1 瓊脂紙錠擴散法(Agar Disc Diffusion Method).......32
3-6溫泉水(酵素)在產品之應用.......34
3-6-1 產品配方設計 .......34
3-6-1-1溫泉水面膜.......34
3-6-1-2溫泉酵素精華液.......36
3-7溫泉水(菌)於人體功效之有效性評估.......37
3-7-1全臉膚質診斷分析儀(VISIA).......37
3-7-2 全臉美白功效性評估儀.......38
3-8-3 ARMA皮膚診斷儀.......41
第四章 結果與討論.......42
4-1溫泉水於人體皮膚之功效性.......42
4-1-1全臉膚質診斷.......42
4-1-1-1 測試一週紫質之變化.......42
4-1-1-2 測試一週棕色斑之變化....... 48
4-1-1-3測試一週斑點之變化.......53
4-1-1-4測試一週紅色區之變化(安全性試驗).......58
4-1-1-5測試一週紫外線色斑之變化.......64
4-1-1-6測試一週毛孔之變化.......69
4-2全臉美白功效性評估儀.......74
4-3ARMA皮膚診斷儀.......81
4-4角質貼片之測試.......89
4-5溫泉水及溫泉菌之活性評估.......92
4-5-1冷凍乾燥之產率....... 92
4-5-2抗氧化能力清除之試驗結果....... 93
4-5-2-1 DPPH .自由基清除試驗結果.......93
4-5-2-2 ABTS.+總抗氧化能力測定試驗結果.......95
4-6溫泉抑菌能力試驗結果.......97
4-6-1以圓盤濾紙擴散法評估中濃縮溫泉0.01mg/0.25mL disc之抑菌圈大小(mm).......99
4-6-2以圓盤濾紙擴散法評估濃縮溫泉0.1mg/0.25mL disc之抑菌圈大小(mm).......102
第五章 結論.......104
第六章 參考文獻.......112

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